通讯机构：
[Li, H ] C;Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.;Key Lab Natl Forestry Grassland Adm Control Artifi, Changsha, Peoples R China.;Hunan Prov Key Lab Control Forest Dis & Pests, Changsha, Peoples R China.;Minist Educ, Key Lab Nonwood Forest Cultivat & Conservat, Changsha, Peoples R China.
关键词：
Colletotrichum fructicola;carbendazim;mechanism of resistance;β-tubulin isotypes
摘要：
<jats:p> Colletotrichum fructicola is the major pathogen of anthracnose in tea-oil trees in China. Control of anthracnose in tea-oil trees mainly depends on the application of chemical fungicides such as carbendazim. However, the current sensitivity of C. fructicola isolates in tea-oil trees to carbendazim has not been reported. Here, we tested the sensitivity of 121 C. fructicola isolates collected from Guangdong, Guangxi, Guizhou, Hainan, Hunan, Jiangsu, and Jiangxi provinces in China to carbendazim. One hundred and ten isolates were sensitive to carbendazim, and 11 isolates were highly resistant to carbendazim. The growth rates, morphology, and pathogenicity of three resistant isolates were identical to those of three sensitive isolates, which indicates that these resistant isolates could form a resistant population under carbendazim application. These results suggest that carbendazim should not be the sole fungicide in control of anthracnose in tea-oil trees; other fungicides with different mechanisms of action or mixtures of fungicides could be considered. In addition, bioinformatics analysis identified two β-tubulin isotypes in C. fructicola: Cfβ<jats:sub>1</jats:sub>tub and Cfβ<jats:sub>2</jats:sub>tub. E198A mutation was discovered in the Cfβ<jats:sub>2</jats:sub>tub of three carbendazim-resistant isolates. We also investigated the functional roles of two β-tubulin isotypes. CfΔ β<jats:sub>1</jats:sub>tub exhibited slightly increased sensitivity to carbendazim and normal phenotypes. Surprisingly, CfΔ β<jats:sub>2</jats:sub>tub was highly resistant to carbendazim and showed a seriously decreased growth rate, conidial production, pathogenicity, and abnormal hyphae morphology. Promoter replacement mutant CfΔ β<jats:sub>2</jats:sub>-2 ×β<jats:sub>1</jats:sub> showed partly restored phenotypes, but it was still highly resistant to carbendazim, which suggests that Cfβ<jats:sub>1</jats:sub>tub and Cfβ<jats:sub>2</jats:sub>tub are functionally interchangeable to a certain degree. </jats:p>
作者机构：
[Zhang, Shengpei; Li, He; Gao, Yalan; Sheng, Song] Key Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha, China;[Zhang, Shengpei; Li, He; Gao, Yalan; Sheng, Song] Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha, China;[Li, He; Sheng, Song] Yuelushan Laboratory Non-wood Forests Variety Innovation Center, Changsha, China
摘要：
Anthracnose, caused by Colletotrichum species, induces significant economic damages to crop plants annually, especially for Camellia oleifera. During infection, the counter-defence mechanisms of plant pathogens against ROS-mediated resistance, however, remain poorly understood. By employing Weighted Gene Co-expression Network Analysis (WGCNA), we identified ACTIVATOR PROTEIN-1 (AP-1), a bZIP transcription factor, as significant to infection. And deletion of CfAP1 inhibited aerial hyphae formation and growth under oxidative stress. Furthermore, RNA-seq analysis post H(2)O(2) treatment revealed 33 significantly down-regulated genes in the AP-1 deficient strain, including A12032, a dual specificity phosphatase (DSP) homologous to MSG5 from Saccharomyces cerevisiae. This ΔCfmsg5 strain showed enhanced oxidative tolerance, reduced ROS scavenging, and negative regulation of the CWI MAPK cascade under oxygen stress, suggesting its involvement in oxidative signal transduction. Importantly, we provide evidence that CfMsg5 regulates growth, endoplasmic reticulum stress, and several unfolded protein response genes upregulated in ΔCfmsg5. Collectively, this study identified core components during C. fructicola infection and highlights a potential regulatory module involving CfAp1 and CfMsg5 in response to host ROS bursts. It provides new insights into fungal infection mechanisms and potential targets like CfAP1 and CfMSG5 for managing anthracnose diseases.
摘要：
Anthracnose is a common disease found in Camellia oleifera producing areas across China, whose primary pathogen is Colletotrichum fructicola. We previously revealed that autophagy is essential for the pathogenicity of C. fructicola. However, the function of ubiquitin-proteasome system (UPS), which is a parallel protein degradation pathway to autophagy, remains elusive. Here, we report that CfRad6, an E2 conjugating enzyme in UPS, interacts with three putative E3 ubiquitin ligases, namely CfRad18, CfUbr1, and CfBre1. Importantly, we presented evidence showing that CfRad6 negatively regulates autophagy, revealing the first link between UPS and autophagy in pathogenic fungi. Targeted gene deletion showed that CfRad6 is involved in growth and conidiation. We further found that the Delta Cfrad6 mutant is defective in appressoria formation and responses to environmental stresses. These combined effects, along with the abnormal autophagy level, lead to the pathogenicity defects of the Delta Cfrad6 mutant. Taken together, our study indicates the pleiotropic functions of CfRad6 in the development and pathogenicity of C. fructicola.
期刊：
International Journal of Pattern Recognition and Artificial Intelligence,2023年37(06):2350013 ISSN：0218-0014
通讯作者：
Hui Li<&wdkj&>Juan Yang
作者机构：
[Hou, Pengfei; Li, Hui; Yang, Zixuan; Chen, Yanyan] Jiangsu Ocean Univ, Dept Comp Sci, Lianyungang 222000, Jinagsu, Peoples R China.;[Zheng, Ying] Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China.;[Yang, Juan] Ningxia Med Univ, Dept Neurol, Gen Hosp, Yinchuan, Ningxia, Peoples R China.
通讯机构：
[Hui Li; Juan Yang] D;Department of Neurology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, P. R. China<&wdkj&>Department of Computer Science, Jiangsu Ocean University, Lianyungang 222000, Jinagsu, P. R. China
关键词：
Entropy;Knowledge based systems;Population dynamics;Semantics;Signal detection;Community detection;Community detection algorithms;Distribution entropies;Interest;Module distribution entropy;Ontology's;Overlapping communities;Overlapping community detections;Topic;Users' interests;Ontology
摘要：
Wood anatomy and plant hydraulics play a significant role in understanding species-specific responses and their ability to manage rapid environmental changes. This study used the dendro-anatomical approach to assess the anatomical characteristics and their relation to local climate variability in the boreal coniferous tree species Larix gmelinii (Dahurian larch) and Pinus sylvestris var. mongolica (Scots pine) at an altitude range of 660 m to 842 m. We measured the xylem anatomical traits (lumen area (LA), cell wall thickness (CWt), cell counts per ring (CN), ring width (RW), and cell sizes in rings) of both species at four different sites Mangui (MG), Wuerqihan (WEQH), Moredagha (MEDG) and Alihe (ALH) and investigated their relationship with temperature and precipitation of those sites along a latitude gradient. Results showed that all chronologies have strong summer temperature correlations. LA extremes were mostly associated with climatic variation than CWt and RWt. MEDG site species showed an inverse correlation in different growing seasons. The correlation coefficient with temperature indicated significant variations in the May-September months at MG, WEQH, and ALH sites. These results suggest that climatic seasonality changes in the selected sites positively affect hydraulic efficiency (increase in the diameter of the earlywood cells) and the width of the latewood produced in P. sylvestris. In contrast, L. gmelinii showed the opposite response to warm temperatures. It is concluded that xylem anatomical responses of L. gmelinii and P. sylvestris showed varied responses to different climatic factors at different sites. These differences between the two species responses to climate are due to the change of site condition on a large spatial and temporal scale.
作者机构：
[Li, He; Wen, Lixia] Cent South Univ Forestry & Technol, Key Lab Natl Forestry & Grassland Adm Control Dis, Changsha 410004, Peoples R China.;[Li, He; Wen, Lixia] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Peoples R China.
通讯机构：
[He Li] K;Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Central South University of Forestry and Technology, Changsha 410004, China<&wdkj&>Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha 410004, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
<jats:p> Colletotrichum fungi could cause anthracnose, a destructive disease in tea-oil trees. The sterol demethylation inhibitor (DMI) tebuconazole has been widely used in controlling plant diseases for many years. However, the baseline sensitivity of Colletotrichum isolates on tea-oil trees to tebuconazole has not been determined. In this study, the sensitivity to tebuconazole of 117 Colletotrichum isolates from tea-oil trees of seven provinces in southern China was tested. The mean effective concentration resulted in 50% mycelial growth inhibition (EC<jats:sub>50</jats:sub>), 0.7625 μg/ml. The EC<jats:sub>50</jats:sub> values of 100 isolates (83%) were lower than 1 μg/ml, and those of 20 isolates (17%) were higher than 1 μg/ml, which implied that resistance has already occurred in Colletotrichum isolates on tea-oil trees. The EC<jats:sub>50</jats:sub> values of the most resistant and sensitive isolates (named Ca-R and Cc-S1, respectively) were 1.8848 and 0.1561 μg/ml, respectively. The resistance mechanism was also investigated in this study. A gene replacement experiment indicated that the CYP51A/B gene of resistant isolates Ca-R and Cf-R1 cannot confer Cc-S1 full resistance to DMI fungicides, although three single point mutants, Cc-S1 CYP51A-T306A and Cc-S1CYP51A-R478K, exhibited decreased sensitivity to DMI fungicides. This result suggested that resistance of Colletotrichum isolates was partly caused by mutations in CYP51A. Moreover, the expression level of CYP51A/B was almost identical among Ca-R, Cf-R1, Cc-S1, and Cc-S1 CYP51A point mutants, which indicated that the resistance was irrelevant to the expression level of CYP51A, and other nontarget-based resistance mechanisms may exist. Our results could help to guide the application of DMI fungicides and be useful for investigating the mechanism of resistance. </jats:p>
作者机构：
[李玲玲; 朱原野; 张盛培; 李河] Key Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Key Laboratory of Forest Bio-resources and Integrated Pest Management for Higher Education in Hunan Province, Central South University of Forestry and Technology, Changsha, 410004, China
期刊：
Surfaces and Interfaces,2023年39:102913 ISSN：2468-0230
通讯作者：
Wan, YW;Li, H
作者机构：
[Wan, Yiwen; Li, Xiaoling; Lei, Qin; Hong, Bo] Hunan Fisheries Sci Inst, Changsha 410153, Peoples R China.;[Li, He; Li, H; Li, Jiaxin] South China Normal Univ, Sch Chem, Guangzhou Key Lab Analyt Chem Biomed, Guangzhou 510006, Peoples R China.;[Lei, Junjie] Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410006, Peoples R China.
通讯机构：
[Li, H ] S;[Wan, YW ] H;Hunan Fisheries Sci Inst, Changsha 410153, Peoples R China.;South China Normal Univ, Sch Chem, Guangzhou Key Lab Analyt Chem Biomed, Guangzhou 510006, Peoples R China.
关键词：
Photoelectrochemical sensor;chlorpyrifos
摘要：
In this study, a simple wet chemical method was used to prepare p-n heterojunction photoelectric composites (Bi2S3@g-C3N4). The p-n junctions were formed to facilitate charge transfer as well as strongly suppress electron -hole pair recombination, thus enhancing the photoelectrochemical (PEC) characteristics. Particularly, according to the transient surface photocurrent response, the photocurrent intensity of the prepared composites was 2.98 times higher relative to the pristine g-C3N4 material. The photocurrent signal revealed the prepared novel PEC sensor composed of Bi2S3@g-C3N4 composite material, which can be used for sensitively and selectively detecting chlorpyrifos. This method presented a linear change from 50 ng/mL to 0.1 mg/mL of chlorpyrifos under the optimal conditions. The linear regression equation was I(mu A)=0.8546-0.1558lgC(mg/mL) (R2=0.9758) and the detection limit was 0.03 ng/mL (S/N=3). In addition, the novel sensing platform with high selectivity could assist in rapidly and effectively detecting chlorpyrifos in environmental analysis.
作者机构：
[姚权; 李司政; 王成玉; 李河] Key Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Key Laboratory of Forest Bio-resources and Integrated Pest Management for Higher Education in Hunan Province, Central South University of Forestry and Technology, Hunan, Changsha, 410004, China
摘要：
<jats:p><jats:italic>Quercus gilva</jats:italic> is an ecologically and economically important species of <jats:italic>Quercus</jats:italic> section <jats:italic>Cyclobalanopsis</jats:italic> and is a dominant species in evergreen broad-leaved forests in subtropical regions of East Asia. In the present study, we reported a high-quality chromosome-scale genome assembly of <jats:italic>Q. gilva</jats:italic>, the first reference genome for section <jats:italic>Cyclobalanopsis</jats:italic>, using the combination of Illumina and PacBio sequencing with Hi-C technologies. The assembled genome size of <jats:italic>Q. gilva</jats:italic> was 889.71 Mb, with a contig number of 773 and a contig N50 of 28.32 Mb. Hi-C scaffolding anchored 859.07 Mb contigs (96.54% of the assembled genome) onto 12 pseudochromosomes, with a scaffold N50 of 70.35 Mb. A combination of <jats:italic>de novo</jats:italic>, homology-based, and transcript-based predictions predicted a final set of 36,442 protein-coding genes distributed on 12 pseudochromosomes, and 97.73% of them were functionally annotated. A total of 535.64 Mb (60.20%) of repetitive sequences were identified. Genome evolution analysis revealed that <jats:italic>Q. gilva</jats:italic> was most closely related to <jats:italic>Q. suber</jats:italic> and they diverged at 40.35 Ma, and <jats:italic>Q. gilva</jats:italic> did not experience species-specific whole-genome duplication in addition to the ancient <jats:italic>gamma</jats:italic> (γ) whole-genome triplication event shared by core eudicot plants. <jats:italic>Q. gilva</jats:italic> underwent considerable gene family expansion and contraction, with 598 expanded and 6,509 contracted gene families detected. The first chromosome-scale genome of <jats:italic>Q. gilva</jats:italic> will promote its germplasm conservation and genetic improvement and provide essential resources for better studying the evolution of <jats:italic>Quercus</jats:italic> section <jats:italic>Cyclobalanopsis</jats:italic>.</jats:p>
作者机构：
[Li, Xi-Ya; Zhangl, Sheng-Pei; He, Li] Cent South Univ Forestry & Technol, Key Lab Natl Forestry & Grassland Adm Control Dis, Changsha 410004, Peoples R China.;[Li, Xi-Ya; He, Li] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Cultivat & Conservat, Minist Educ, Changsha, Peoples R China.;[Zhangl, Sheng-Pei; He, Li] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha, Peoples R China.
通讯机构：
[Li He] K;Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Central South University of Forestry and Technology, Changsha, China<&wdkj&>Key Laboratory for Non-wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, Changsha, China<&wdkj&>Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha, China
摘要：
Tea oil is widely used as edible oil in China, which extracted from the seeds of Camellia oleifera. In China, the national oil-tea camellia planting area reached 4.533 million hectares, the output of oil-tea camellia seed oil was 627 000 tons, and the total output value reached 18.3 billion dollars. Anthracnose is the common disease of Ca. oleifera, which affected the production and brought huge economic losses. Colletotrichum fructicola is the dominant pathogen causing anthracnose in Ca. oleifera. The retromer complex participates in the intracellular retrograde transport of cargos from the endosome to the trans-Golgi network in eukaryotes. Vacuolar protein sorting 35 is a core part of the retromer complex. This study aimed to investigate the role of CfVps35 in C. fructicola. The CfVPS35 gene was deleted, resulting in reduced mycelial growth, conidiation, and response to cell wall stresses. Further analysis revealed that CfVps35 was required for C. fructicola virulence on tea oil leaves. In addition, the ΔCfvps35 mutant was defective in glycogen metabolism and turgor during appressorium development. This study illustrated that the crucial functions of CfVps35 in growth, development, and pathogenicity.
期刊：
Journal of Fungi,2022年8(8):835- ISSN：2309-608X
通讯作者：
He Li
作者机构：
[Li, He; Li, Xiya; Li, Sizheng] Cent South Univ Forestry & Technol, Grassland Adm Control Artificial Forest Dis & Pes, Key Lab Natl Forestry, Changsha 410004, Peoples R China.;[Li, He] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Peoples R China.
通讯机构：
[He Li] K;Key Laboratory of National Forestry, Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Central South University of Forestry and Technology, Changsha 410004, China<&wdkj&>Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha 410004, China<&wdkj&>Author to whom correspondence should be addressed.
作者机构：
[Li, He; Li, Lingling] Cent South Univ Forestry & Technol, Key Lab Natl Forestry & Grassland Adm Control Dis, Changsha, Peoples R China.;[Li, He; Li, Lingling] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha, Peoples R China.;[Li, He; Li, Lingling] Cent South Univ Forestry & Technol, Key Lab Non Wood Forest Cultivat & Conservat, Minist Educ, Changsha, Peoples R China.
通讯机构：
[He Li] K;Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Central South University of Forestry and Technology, Changsha, China<&wdkj&>Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha, China<&wdkj&>Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, Changsha, China
关键词：
Colletotrichum aeschynomenes;identification;inoculation trial;tea-oil tree
摘要：
Anthracnose is a major disease of cultivated Camellia oleifera (tea-oil tree) in China, with serious negative impacts on the yield and quality of cooking oil. In 2020, symptoms of anthracnose were observed on the leaves of tea-oil trees in Hunan province, and Colletotrichum isolates were obtained from symptomatic leaf tissues. Morphological characterization and sequencing of ApMat gene region indicated that these isolates represented Colletotrichum aeschynomenes. Inoculation trial confirmed that C. aeschynomenes causes anthracnose on Ca. oleifera in China.
作者机构：
[陈振宏; 李河; 陈嘉怡; 陈晓迪; 罗晶; 张盛培] Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, Hunan, Changsha, 410004, China
作者机构：
[Zhang, Shengpei; Li, He; Li, Sizheng] Cent South Univ Forestry & Technol, Key Lab Natl Forestry, Changsha, Peoples R China.;[Zhang, Shengpei; Li, He; Li, Sizheng] Cent South Univ Forestry & Technol, Grassland Adm Control Dis & Pests South Plantat, Changsha, Peoples R China.;[Zhang, Shengpei; Li, He; Li, Sizheng] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha, Peoples R China.;[Li, He; Li, Sizheng] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Nonwood Forest Cultivat & Conservat, Changsha, Peoples R China.
通讯机构：
[He Li] K;Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Central South University of Forestry and Technology, Changsha, China<&wdkj&>Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Central South University of Forestry and Technology, Changsha, China<&wdkj&>Key Laboratory for Non-wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, Changsha, China
关键词：
Camellia oleifera;Colletotrichum fructicola;pathogenicity;vacuolar protein sorting 39
摘要：
<jats:title>Abstract</jats:title><jats:p>The tea‐oil tree <jats:italic>Camellia oleifera</jats:italic> is native to China and is cultivated in many parts of southern China. Anthracnose on <jats:italic>Ca</jats:italic>.<jats:italic>oleifera</jats:italic> is one of the most serious diseases in China. The major causal pathogen of tea‐oil anthracnose is <jats:italic>Colletotrichum fructicola</jats:italic>, but the detailed molecular mechanisms of its pathogenicity are still largely unclear. Vacuolar protein sorting 39 (Vps39) is a component of the homotypic fusion and vacuole protein sorting (HOPS) complex. To investigate the role of HOPS proteins in the fungal plant anthracnose pathogen <jats:italic>C</jats:italic>.<jats:italic>fructicola</jats:italic>, the <jats:italic>CfVPS39</jats:italic> gene was deleted. This resulted in reduced mycelial growth, conidial production and complete loss of pathogenicity on tea‐oil leaves and fruits. In addition, ∆<jats:italic>Cfvps39</jats:italic> showed increased sensitivity to cell wall stress, osmotic stress and endoplasmic reticulum stress. Further analysis revealed that CfVps39 is required for appressorium formation and homotypic vacuole fusion, both important for fungal pathogen invasion. Our study provides evidence that CfVps39 has a role in conidiation, stress response, appressorium formation, homotypic vacuole fusion and virulence against tea‐oil. This research not only provides the theories for revealing the pathogenic molecular mechanism of <jats:italic>C</jats:italic>.<jats:italic>fructicola</jats:italic>, but also has important guiding significance to reveal new fungicide targets to control this devastating disease.</jats:p>
作者机构：
[李玲玲; 张盛培; 李河] Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, Hunan, Changsha, 410004, China
