期刊论文

MAPKK-encoding gene CfMKK1 in Colletotrichum fructicola is required for its growth and pathogenicity

中文

微生物学报

0001-6209

2021

61

1

141-151

10.13343/j.cnki.wsxb.20200124

2019JJ40531:湖南省自然科学基金 31570641:国家自然科学基金

本校为第一机构

【目的】由果生炭疽菌引起的炭疽病是油茶的主要病害,造成油茶产量下降。本文研究果生炭疽菌中丝裂原活化蛋白激酶CfMkk1的生物学功能,旨在为解析油茶炭疽病菌的致病机理提供依据。【方法】根据同源重组原理构建CfMKK1基因敲除载体片段,采用PEG介导法将载体导入原生质体中筛选获得突变体菌株DCfmkk1;PCR扩增果生炭疽菌含有启动子的CfMKK1基因回补片段,构建回补载体pYF11::CfMKK1;采用PEG介导法把回补载体转化至突变体的原生质体中,荧光筛选回补菌株??Cfmkk1-C。测定野生型菌株、突变体菌株DCfmkk1及基因回补菌株?Cfmkk1-C在营养生长、附着胞形成、胁迫应答和致病力等生物学表型。【结果】与野生...

[Objective] Colletotrichum fructicola is an important pathogen that results in yield loss of Camellia oleifera. We studied the functions of the mitogen-activated protein kinase gene CfMKK1 in C. fructicola for analyzing the pathogenic mechanism of oil-tea tree anthracnose. [Methods] The homologous recombination method was used to construct the CfMKK1 gene-deleted fragment, which was transformed into the protoplasts generated by using PEG-mediated method to obtain the mutant strain ΔCfmkk1. The PCR-amplified CfMKK1 gene-containing complement of...