期刊论文

中文

菌物学报

1672-6472

2020

39

10

1886-1896

10.13346/j.mycosystema.200081

Supported by Natural Science Foundation of Hunan Province (2019JJ40531), the National Natural Science Foundation of China (31570641), and Hunan Provincial Innovation Foundation for Postgraduate (CX20190623). Corresponding author. E-mail: csuftlihe@163.com Received: 2020-03-17, accepted: 2020-05-04

本校为第一机构

果生刺盘孢Colletotrichum fructicola是油茶炭疽病优势病原菌。前期研究发现bZIP转录因子CfHac1参与调控该菌的生长发育和致病性。为了揭示转录因子CfHac1调控果生刺盘孢响应内质网压力和致病机理,本研究测定了ΔCfhac1突变体对内质网压力胁迫剂的敏感性,发现突变体对二硫苏糖醇（dithiothreitol,DTT）的耐受性下降,说明CfHAC1基因可能参与调控果生刺盘孢响应内质网压力胁迫过程。进一步利用高通量RNA-seq技术对该病菌野生型菌株和CfHAC1敲除突变体菌株在DTT胁迫下的转录组进行了比较分析,结果表明差异表达基因共有2 680个,其中上调表达基因有1 181个,下调表达基因有1 499个。Gene Ontology功能...

Colletotrichum fructicola is a major pathogen causing anthracnose on Camellia oleifera. In previous study, we characterized that the bZIP transcription factor CfHac1 controlled the development and pathogenicity of C. fructicola. To identify the role of CfHac1 in the endoplasmic reticulum (ER) stress in C. fructicola, the growth of a ΔCfhac1 mutant strain of C. fructicola was determined on the PDA medium containing dithiothreitol, an ER stress factor. The results revealed that the ΔCfhac1 strain was sensitive to dithiothreitol. To uncover the ...