摘要：
Tea oil comes from unique woody oil trees in South China and is one of the four woody edible oils in the world. Tea oil is the edible oil obtained by squeezing mature seeds of oil tea. The unsaturated fatty acid content in tea oil can reach as much as 90%, which is the highest amount so far for unsaturated fatty acid in edible oil. The content of catechin in oil tea is about 80%, which have good whitening and anti-oxidation effects. Tea polyphenol is a natural antioxidant with significant antioxidation, which can eliminate free radicals, protect cell membrane structures, etc. Camellia saponin has many functions: Anti-permeability, anti-inflammatory, analgesic, anticancer, etc. Squalene is a kind of polyphenolic active composition with good oxygen-enriched capacity in oil tea. This paper summarizes the fatty acid composition and some functional components of tea oil, and its functions of anti-tumor, lipid-lowering, liver and heart protection, antisepsis and anti-inflammation, coronary heart disease prevention, delay of atherosclerosis, anti-oxidation, and regulation of the immune function. This paper aims to lay the foundation for further study and use of the medicine and health functions of tea oil.
作者机构：
[Zhou Guoying; Zhang Huaiyun; Li He] Cent S Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Hunan, Peoples R China.
通讯机构：
[Li He] C;Cent S Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Hunan, Peoples R China.
会议名称：
International Conference on Micro Nano Devices, Structure and Computing Systems
会议时间：
NOV 06-07, 2010
会议地点：
Int Sci & Engn Ctr, Singapore, SINGAPORE
会议主办单位：
Int Sci & Engn Ctr
会议论文集名称：
Advanced Materials Research
关键词：
Bamboo timber;Environmental protection type fiber material;Natural bamboo fiber;Research status
摘要：
The natural bamboo fiber is a green environmental protection type fiber material extracted from bamboo. Compared with other fibers, it has better permeability, unique resilience, transient water absorption, antibacterial activity, deodorant activity, and a strong lateral and longitudinal strength. This paper reviews the structure of natural bamboo fiber and its characteristics, physical morphology, chemical composition, hygroscopicity and moisture liberation, antibacterial activity, anti-ultraviolet function, spinning performance of the development and utilization status of bamboo fiber, which provides reference for the research and utilization of natural bamboo fiber.
摘要：
Chitin deacetylase (CDA) catalyzes the conversion of chitin to chitosan by the deacetylation of N-acetyl-D-glucosamine residues. In this study, 9 strains of bacteria with chitin deacetylase activity were isolated from canal mud samples from the Xiangjiang River in Changsha, Hunan Province. Of these 9 strains, the Z7 strain had the highest chitin deacetylase activity, as determined by enzyme assay screening. Based on its culture, morphological, physiological characteristics and molecular identification, strain Z7 was identified as Bacillus amyloliquefaciens. Yeast extract at a concentration of 1.0% was an ideal nitrogen source for production of CDA (16.78 Eu/ml). Starch at 2% functioned best as the major carbon source for optimum production of CDA (17.84 Eu/ml). Magnesium sulfate at a concentration of 0.04% was an ideal inorganic salt for CDA production (17.45 Eu/ml). Ideal pH and temperature for optimum production of CDA by this strain were pH 6 and 37 degrees C.
摘要：
Pycnoporus cinnabarinus is a plant pathogen. It is common in many areas and is widely distributed throughout the world. Laccases of are some of the few oxidoreductases commercialized as industrial catalysts. In the present study, some characters of the amino acid sequence of P.cinnabarinus laccase (Lac1) were predicted and analyzed with the tools of bioinformatics. These results showed that the protein was composed of 20 kinds of amino acid; the theoretical pI of manganese peroxidase was 4.81 and the theoretical molecular weight of manganese peroxidase was 56292.0 Da; total number of atoms was 7806; the extinction coefficient was 58120 (280 nm). The N-terminal of the sequence considered was M (Met) and the estimated half-life was 30 hours (mammalian reticulocytes, in vitro). The instability index (II) was computed to be 34.50; this classifies the protein as stable. Aliphatic index was 82.64. Grand average of hydropathicity (GRAVY) was -0.063. There were 8 glycosylation sites, a signal peptide and conserved domains.
摘要：
Gibberella moniliformis is a common disease in C. oleifera, and always results in significant economic losses. Phosphoenolpyruvate carboxykinase is a critical gluconeogenic enzyme and catalyzes the first committed step in the diversion of tricarboxylic acid cycle intermediates toward gluconeogenesis. According to the relative conservation of homologous gene, a bioinformatics strategy was applied to clone phosphoenolpyruvate carboxykinase gene of Gibberella moniliformis by blasting search of EST database with homologous gene cDNA of Neurospora crassa and identified. Some characters of the PEPCK that can encode amino acid were analyzed and predicted by the tools of bioinformatics in the following aspects, including the composition of amino acid sequences, physical and chemical, O-glycosylation site and tertiary structure of protein and function. These results showed that the full-length of PEPCK was 1962 bp and it contained a complete ORF (1671bp), encoded 556 amino acids, which are much conserved in ascomycetes. The calculated molecular weight of PEPCK was 61893.1 Da, theoretical pI of 5.77, 20 a-helix, 37 sheets, 7 glycosylation sites; it was a stable protein with active site ATP -binding site, metal-binding site and substrate-binding site.
期刊：
2009 INTERNATIONAL CONFERENCE ON ENVIRONMENTAL SCIENCE AND INFORMATION APPLICATION TECHNOLOGY, VOL III, PROCEEDINGS,,2009年:273-276
通讯作者：
Zhou Guoying
作者机构：
[He Yuanhao; Zhou Guoying; Li Lin; Li He] Cent S Univ Forestry & Technol, Coll Forestry, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhou Guoying] C;Cent S Univ Forestry & Technol, Coll Forestry, Changsha, Hunan, Peoples R China.
关键词：
Heavy metal;soil;microorganism-plant;bioremediation
摘要：
Heavy metal contaminated soil problem has already become the global focus. With the increasing attention to the environment protection, scientist began to search new methods for remediation of contaminated soil. This paper mainly studied the effect of microorganism-plant combined bioremediation on contaminated soil in the industrial district, hoping to provide an effective bioremediation technique for heavy metal contaminated soil. In this research, 5 strains which were screened from rhizosphere resistance microorganism combined with Ligustrum quihoui Carr. were used for pot experiments, the results showed that 5 strains all had promotion effect on seedling growth in heavy metal contaminated soil, and high, root, and fresh weight were all higher than that of the control seedling groups. The microorganism-plant combined bioremediation system improved the plant remediation effect to heavy metal Zn, Cd, especially when 5 strains were coexisted and co-actived showed the obvious remediation effect. For Zn, its absorption coefficient was 0.51 and transmission coefficient was 1.55 and for Cd its absorption coefficient was 0.41 and its transmission coefficient was 0.48 when it was under the Zn, Cd combined pollution soil. So it has a good effect of microorganism-plant combined remediation.
摘要：
The study on molecular structure of Fusarium proliferatum clinically isolated can provide the basis for accurate diagnosis and treatment of F. proliferatum infection. The rDNA ITS gene of 6 strains that had been identified as Fusarium proliferatum based on morphological were cloned and sequenced. The molecule phylogenetic tree was constructed based on rDNA ITS sequences, and the 6 genus were divided into two groups. GroupI was F.proliferatum, including strains of CSUFT201, CSUFT204, CSUFT205. Group II was F. moniliforme, including CSUFT202,CSUFT203,CSUFT206. The results of this study showed that 3 strains of F. moniliforme were confirmed out of the 6 strains with the molecular methods.
摘要：
Camellia oleifera is an economical important plant in southern China for edible oil production. Anthracnose is a serious disease that limited its development. The internal transcribed spacer (ITS) regions and the 5.8S rRNA gene of strain C1 of the pathogenic fungus Colletetrichum gloeosporioides were sequenced in order to design specific PCR primers for pathogen detection. Alignment of the sequence data of strain C1 and the other Colletetrichum species obtained from the Genbank were made using CLUSTAL W. Based on the aligned ITS sequences, specific primers for C. gloeosporioides were developed (YT1 and YT2). The infecting pathogens were successfully detected with our specific primer set and showed high specificity. The result showed that the nested-PCR reaction was at least 10,000-fold more specific than that of the simple PCR method. This new method provides a useful technique to further study disease cycle and for early prediction of anthracnose of Camellia oleifera.
通讯机构：
[Zhou, Guoying] C;Cent S Univ Forestry & Technol, Biotechnol Core Facil, Changsha 410004, Hunan, Peoples R China.
关键词：
Camellia oleifera;anthracnose;antagonistic bacteria;biological control
摘要：
Anthracnose is a common Camellia oleifera disease caused by Colletetrichum gloeosporioide. This study was to isolate antagonistic bacteria from Camellia oleifera tissues to control the anthracnose. Antagonistic bacteria was screened from 175 endophytic bacteria with confront culture method. The results of the study showed that the antagonistic effect of YB128 strain was the best with 86.1% inhibitory rate. According to the results of the researching on morphology, cultural characteristics, physiological and biochemical propertices, 16S gene sequence and molecular phylogenetic tree, YB128 was identified as Bacillus licheniformis.
