期刊论文

Pathogen of Oil-Tea Trees Anthracnose Caused by Colletotrichum spp. in Hunan Province

Zhou, Guoying

中文

林业科学

1001-7488

2017

53

8

43-53

10.11707/j.1001-7488.20170806

国家自然科学基金项目（31570641） 教育部农作物生物灾害综合治理重点实验室/农业部华东作物有害生物综合治理重点实验室开放课题

本校为第一且通讯机构

【目的】研究湖南省油茶炭疽病病原,为该病害的防控提供依据。【方法】采集湖南省长沙、株洲、浏阳、永州、怀化、常德、常宁等油茶主产区9个样地的典型油茶炭疽病叶,分离纯化叶片病健交界处的真菌菌株,观察菌株在（PDA）培养基上的菌落、分生孢子及附着孢的形态特征,确定为炭疽属真菌。进一步对炭疽属菌株进行柯赫氏法则验证,确定油茶炭疽病病原菌菌株。对获得病原菌的r DNA-ITS、CAL和GAPDH 3个基因进行PCR扩增和测序,得到的基因序列与GenBank中已有模式炭疽属菌株序列按照3个基因一致的顺序拼接后采用PAUP和MrBayes软件构建系统发育树。【结果】从湖南省9个采样地共分离获得62株炭疽属菌株,致...

Objective: The filamentous fungi, Colletotrichum spp., are found in all five continents and are capable of causing severe diseases in the important plants such as Camellia oleifera (oil-tea trees). The pathogen causes defoliation, as well as necrotic spots on fruits. In recent years, the disease is rapidly spreading in the oil-tea trees producing areas of southern China. The objective of this study is to identify the pathogen causing stem and leaf anthracnose on oil-tea trees in Hunan, and to provide a basis for disease control and resistance b...