摘要:
Native and acetylated broken-rice starches (nanocrystals) with different degrees of substitution (DS) and their corresponding films were individually prepared, and the drug release profiles, weight loss, solubility and dispersion and surface morphology were comparatively studied. Bovine serum albumin (BSA) was used as a model drug. Acetylated native starch (ANS) DS 2.58, acetylated starch nanocrystals (ASN) DS 0.98, ASN DS 1.86, and ASN DS 2.72 were observed to be very soluble in chloroform. BSA was released rapidly from the native rice starch (NS) and ANS DS 2.58 films. ASN with high DS significantly slowed down the release of BSA from films, the percentages of BSA released from film ASN DS 2.72 only reached to 13% after 3.5?weeks release, and the release data followed Korsmeyer-Peppas equation. Further studies reveal that the particle size of ASN DS 2.72 was smallest, and the weight loss of ASN DS 2.72 film was lowest. The results demonstrate that acetylation and nanometer particle form of rice starch film can effectively retard protein drug release, and the prepared films based on ASN with high DS from broken rice may be suitable for the controlled protein delivery. (C) 2019 Elsevier B.V. All rights reserved.
摘要:
Curcumin, a yellow pigment present in the spice turmeric (Curcuma longa), has been linked with various bioactivities, but its optimum potential is limited by its lack of dispersibility in aqueous solvents and poor oral bioavailability. Here, we employed a protein-based nanoparticle approach to improve bioactivity and bioavailability. Curcumin was encapsulated with 95.94% efficiency in biodegradable nanoparticulate formulation based on rice bran albumin (RBA). The mean particle diameter and zeta-potential of curcumin-loaded RBA nanoparticles (Cur-RBA-NPs) were 120 nm and -36.3 mV, respectively. The in vitro bioactivity and in vivo bioavailability of Cur-RBA-NPs were evaluated. The results indicated that the in vitro bioactivities (antioxidant activity, anti-inflammatory activity, and anti-proliferative activity on tumor cells) of Cur-RBA-NPs were superior to those of free curcumin, respectively. Moreover, Cur-RBA-NPs significantly enhanced the bioavailability of curcumin in rats as compared with free curcumin. Besides, the results clearly indicated the promise of RBA-based nanoparticles for oral delivery of poorly bioavailable molecules like curcumin. (C) 2017 Elsevier Ltd. All rights reserved.
摘要:
In this study, we developed a new nanoparticulate system for acetylated starch nanocrystals (ASN) using broken rice. ASN with different degrees of substitution (DS) of 0.04, 0.08 and 0.14 were prepared using acetic anhydride as acetylating agent through reaction with starch nanocrystals (SN). The resulting ASN were investigated for the capability to load and release doxorubicin hydrochloride (DOX), and the antitumor activities of DOX-loaded SN and DOX-loaded ASN were evaluated as potential drug delivery systems for cancer therapy. Cellular uptake and cytotoxicity of nanocrystals and the DOX-loaded nanocrystals were investigated using fluorescence microscopy and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. Compared with acetylated starches (AS) and native starches (NS), ASN with DS 0.14 loaded up to 6.07% of DOX with a higher loading efficiency of 91.1% and had steadier drug-release rates. Toxicity analysis using the rat hepatocytes model suggested that ASN was biocompatible and could be used for drug delivery. Furthermore, ASN were taken up by cancer cells in vitro and significantly enhanced the cytotoxicity of DOX against HeLa human cervical carcinoma cells. The IC50 value of DOX-loaded ASN-DS 0.14 was 3.8 mu g/mL for 24 h of treatment, which was significantly lower than that of free DOX (21 mu g/mL). These results indicate that the prepared ASN using broken rice is a promising vehicle for the controlled delivery of DOX for cancer therapy. (C) 2016 Elsevier B.V. All rights reserved.
期刊:
Advance Journal of Food Science and Technology,2013年5(1):46-53 ISSN:2042-4868
通讯作者:
Wu, W.
作者机构:
[Ying Liang; Yue Wu; Wei Wu; Qinlu Lin; Xiangjin Fu; Huaxi Xiao] Department of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China;[Yufei Hua] Department of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China;[Ying Liang; Yue Wu; Wei Wu; Qinlu Lin; Xiangjin Fu; Huaxi Xiao] National Engineering Laboratory for Rice and By-Product Deep Processing, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China
通讯机构:
[Wu, W.] D;Department of Food Science and Engineering, Central South University of Forestry and Technology, China
关键词:
Aggregation;Lipid peroxidation product;Mechanism;Protein oxidation;Soy protein
摘要:
Native rice starch lacks the versatility necessary to function adequately under rigorous industrial processing, so modified starches are needed to meet the functional properties required in food products. This work investigated the impact of enzymatic hydrolysis and cross-linking composite modification on the properties of rice starches. Rice starch was cross-linked with epichlorohydrin (EPI) with different concentrations (0.5%, 0.7%, 0.9% <em>w/w</em>, on a dry starch basis), affording cross-linked rice starches with the three different levels of cross-linking that were named R<sub>1</sub>, R<sub>2</sub>, and R<sub>3</sub>, respectively. The cross-linked rice starches were hydrolyzed by α-amylase and native, hydrolyzed, and hydrolyzed cross-linked rice starches were comparatively studied. It was found that hydrolyzed cross-linked rice starches showed a lower the degree of amylase hydrolysis compared with hydrolyzed rice starch. The higher the degree of cross-linking, the higher the capacity to resist enzyme hydrolysis. Hydrolyzed cross-linked rice starches further increased the adsorptive capacities of starches for liquids and decreased the trend of retrogradation, and it also strengthened the capacity to resist shear compared to native and hydrolyzed rice starches.