通讯机构：
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Key Lab of Non-Wood Forest Product of State Forestry Administration, Central South University of Forestry and Technology, Changsha, China
通讯机构：
The Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China
作者：
Cao, Heping*;Zhang, Lin;Tan, Xiaofeng;Long, Hongxu;Shockey, Jay M.
期刊：
PLOS ONE,2014年9(2):e88409 ISSN：1932-6203
通讯作者：
Cao, Heping
作者机构：
[Shockey, Jay M.; Cao, Heping] ARS, USDA, So Reg Res Ctr, Commod Utilizat Res Unit, New Orleans, LA 70130 USA.;[Long, Hongxu; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha, Hunan, Peoples R China.
通讯机构：
[Cao, Heping] A;ARS, USDA, So Reg Res Ctr, Commod Utilizat Res Unit, New Orleans, LA 70130 USA.
摘要：
Triacylglycerols (TAG) are the major molecules of energy storage in eukaryotes. TAG are packed in subcellular structures called oil bodies or lipid droplets. Oleosins (OLE) are the major proteins in plant oil bodies. Multiple isoforms of OLE are present in plants such as tung tree (Vernicia fordii), whose seeds are rich in novel TAG with a wide range of industrial applications. The objectives of this study were to identify OLE genes, classify OLE proteins and analyze OLE gene expression in tung trees. We identified five tung tree OLE genes coding for small hydrophobic proteins. Genome-wide phylogenetic analysis and multiple sequence alignment demonstrated that the five tung OLE genes represented the five OLE subfamilies and all contained the "proline knot" motif (PX5SPX3P) shared among 65 OLE from 19 tree species, including the sequenced genomes of Prunus persica (peach), Populus trichocarpa (poplar), Ricinus communis (castor bean), Theobroma cacao (cacao) and Vitis vinifera (grapevine). Tung OLE1, OLE2 and OLE3 belong to the S type and OLE4 and OLE5 belong to the SM type of Arabidopsis OLE. TaqMan and SYBR Green qPCR methods were used to study the differential expression of OLE genes in tung tree tissues. Expression results demonstrated that 1) All five OLE genes were expressed in developing tung seeds, leaves and flowers; 2) OLE mRNA levels were much higher in seeds than leaves or flowers; 3) OLE1, OLE2 and OLE3 genes were expressed in tung seeds at much higher levels than OLE4 and OLE5 genes; 4) OLE mRNA levels rapidly increased during seed development; and 5) OLE gene expression was well-coordinated with tung oil accumulation in the seeds. These results suggest that tung OLE genes 1-3 probably play major roles in tung oil accumulation and/or oil body development. Therefore, they might be preferred targets for tung oil engineering in transgenic plants.
作者：
Huang, Liyuan;Tan, Xiaofeng;Long, Hongxu;Jiang, Nan
期刊：
Journal of Chemical and Pharmaceutical Research,2013年5(12):1252-1257 ISSN：0975-7384
通讯作者：
Tan, X.
作者机构：
[Long, Hongxu; Huang, Liyuan; Tan, Xiaofeng; Jiang, Nan] Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Central South University of Forestry and Technology, Ministry of Education, Changsha, China;[Long, Hongxu; Huang, Liyuan; Tan, Xiaofeng; Jiang, Nan] The Key Lab of Non-wood Forest Product of Forestry Ministry, Central South University of Forestry and Technology, Changsha, China
摘要：
Taking fruit expanding period and oil synthetic peak period of Oiltea camellia (Camellia oleifera Abel.) seeds as materials to build transcriptome and expression profile database, it is indicated that there are totally 104 non-redundant gene sequences involved in fatty acid biosynthesis of Oiltea camellia and 14 main enzyme genes involved after a comprehensive analysis on transcriptome and expression profile database. Taking KEGG database as a reference, we analyzed the biosynthetic routes of fatty acid of Oiltea camellia and drawn its route graph, which revealed the regulation of key enzyme genes and the basic catalytic rule of fatty acid biosynthesis in Oiltea camellia, providing certain scientific basis for oil content increasing and fatty acid components by ways of genetic engineering.
通讯机构：
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, China
