期刊论文

中文

江西农业大学学报

1000-2286

2013

35

5

962-969

10.3969/j.issn.1000-2286.2013.05.012

国家林业公益性行业科研专题（200904023）； 国家林业公益性行业科研专项重点项目（201204403）；

本校为第一机构

以葡萄桐近成熟种子为材料,根据油桐转录组测序分析结果设计引物,利用RT-PCR技术克隆获得了2条KAR基因的全长cDNA,分别命名为Vf_KAR1和Vf _KAR2(GenBank登录号:KF025646,KF025647).生物信息学分析结果表明,Vf_KAR1和Vf_KAR2基因的CDS长度分别为993 bp和900 bp,分别编码331个和300个氨基酸;与其它物种的KAR氨基酸序列具有较高的相似性,Vf_KAR1与蓖麻的相似度最高,达82％;Vf_KAR2基因与花生、辣椒的相似度高达76％;2条KAR基因的多肽都含叶绿体转运肽,但是都没有信号肽;Vf_KAR1有1个明显的跨膜螺旋拓扑结构和1个疑似跨膜螺旋拓扑结构,Vf_KAR2有2个疑似的跨膜螺旋拓扑结构;肽链都表现为疏水性,都有典...

Primers were designed according to the results of the transcriptome sequencing analysis of Vernicia fordii,two full-lengths KAR cDNA were cloned by RT-PCR technique from nearly ripe seed in Vernicia fordii,which were respectively named Vf_KAR1 and Vf_KAR2 （GenBank accession number：KF025646,KF025647）.The bioinformatics analysis showed that the CDS lengths of Vf_KAR1 and Vf_KAR2 genes were 993 bp and 900 bp respectively,coding 331 and 300 amino acids;the two sequences had high similarity with other species,Vf_KAR1 had the highest similarity wi...