会议论文

Cloning and sequence analysis of full-length cDNA encoding beta-ketoacyl-ACP synthaseⅢ from Vernicia fordii

中文

2012

1-9

中国林学会经济林分会2012年学术年会

中国林学会经济林分会2012年学术年会论文集

2012-11-01

浙江临安

中国林学会

本校为第一机构

　　β-酮脂酰-ACP合酶－KAS是脂肪酸合成中的一种缩合酶.本研究以近成熟的油桐种子为材料,利用改良CTAB法提取总RNA,并根据GenBank 中蓖麻等已知的β-酮脂酰-ACP合酶基因KASⅢ 的序列设计引物,利用简并PCR、RACE 等技术克隆得到油桐种子中KASⅢ基因全长cDNA.该基因序列全长1901bp,开放阅读框为1209bp,编码403个氨基酸,通过与其他物种的KASⅢ氨基酸序列比对,油桐KASⅢ基因与与麻疯树的同源性最高,达90％.软件预测表明,KASⅢ蛋白等电点为5.78,存在跨膜结构域,整个蛋白质基本上是表现出亲水性的.

　　beta-ketoacyl-ACP synthase is fatty acid synthesis on condensation effect of enzyme, Total RNA were successfully extracted by developing Vernicia fordii seeds with improved CTAB method . Primer was Designed according to the KASⅢ sequences recorded in Genbank.Full-Length cDNA of KASⅢ was obtained By cloning it with PCR 、RACE method.It was 1901 bp in length and contained a 1209 bp ORF (open reading frame) encoding a peptide of 403 amino acids,Amino Acid sequence aligenment with other species showed that the KASⅢof V.fordii displayed the h...