期刊论文

Tan, X.-F.

中文

植物生理与分子生物学学报

1671-3877

2013

49

11

1245-1249

10.13592/j.cnki.ppj.2013.11.012

国家林业公益性行业科研专项

本校为第一且通讯机构

以油桐无菌苗叶片为试材, 研究不同种类及浓度的植物生长调节剂对愈伤组织诱导、分化、增殖及生根的影响。结果表明: 叶片愈伤组织的最佳诱导培养基为1/2MS+2.0 mg·L~(-1) 6-BA+1.0 mg·L~(-1) 2,4-D, 诱导率达100%; 最佳分化培养基为1/2MS+3.0 mg·L~(-1) 6-BA+0.1 mg·L~(-1) IBA+0.05 mg·L~(-1) IAA, 分化率为86.36%; 最佳继代增殖培养基为MS+3.0 mg·L~(-1) 6-BA+0.05mg·L~(-1) IBA; 最佳生根培养基为1/2MS+0.1 mg·L~(-1) IBA, 生根率93.83%。炼苗后移栽到泥炭土:珍珠岩:蛭石=2:1:1的基质中, 成活率达92%以上。

The effects of different concentrations and combinations of plant growth regulators on callus induction, shoot differentiation, proliferation and root formation were studied using germ-free leaves of tung tree (Vernicia fordii) as plant materials. The results showed that the fittest medium for callus induction from leaves were 1/2MS+2.0 mg·L-1 6-BA+1.0 mg·L-1 2, 4-D, with a 100% callus induction rate; the fittest medium for shoot differentiation were 1/2MS+3.0 mg·L-1 6-BA+0.1 mg·L-1 IBA+0.05 mg·L-1 IAA, with a 86.36% differentiation rate; ...