摘要：
Phellodendron chinense Schneid is an important Chinese herb with berberine and phellodendrine in stems and leaves, but with little information available on in vitro culture of this species. Disinfection of explants in 75% alcohol for 45 s, sterilization in 0.1% HgCl2 for 20 min, and submersion in 1.0 mol L-1 gibberellin3 (GA(3)) solution for 24 h was the optimal condition for seed germination. Murashige and Skoog's (MS) medium supplemented with 2.0 mg L-1 6-benzylaminopurine (6-BA) in combination with 1.5 mg L-1 1-naphthylacetic acid (NAA) was optimal for callus induction. MS medium supplemented with 2.0 mg L-1 6-BA was the appropriate medium for induction of adventitious shoots, and 1/2MS medium supplemented with 2.0 mg L-1 indole-3-butytric acid (IBA) and 0.5% active carbon was the optimal medium for root induction. The 15-d survival rate of regenerated plantlets after transplanting to basins containing perlite and peat moss (1:4) was greater than 80%, and the berberine and phellodendrine accumulation was lower in callus compared with regenerated plantlets. The establishment of highly efficient regeneration system provides technical support for genetic breeding of Phellodendron chinense Schneid.
摘要：
Using Phellodendron chinense seedlings as material, and treated with different concentrations of exogenous 6-Benzylaminopurine (6-BA) and alpha-naphthyacetic acid (NAA), then observed the growth status. Furthermore, we detected the contents of chlorophyll and soluble sugar, the activities of antioxidases by spectrophotometry, and determined the contents of secondary metabolite by high performance liquid chromatograph. The results showed that different concentrations of exogenous 6-BA increases the fresh weights and plant heights of Phellodendron chinense seedlings, and enhances the contents of chlorophyll and soluble sugar. NAA promoted growth, but deduced the contents of soluble sugar. Compared with control, culturing for 40 d, proper concentrations 6-BA enhanced the activity levels of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), proper concentrations NAA increased the activity levels of SOD and CAT, but decreased the levels of POD compared with CK. Suitable concentrations 6-BA enhanced contents of berberine, phellodendrine and palmatine in stems, proper concentrations NAA increased contents of berberine and phellodendrine, but deduced contents of palmatine compared with CK. Based on these results, we concluded that the exogenous 6-BA and NAA had key regulation on the growth and contents of medicinal ingredient of Phellodendron chinense seedlings. (C) 2017 The Authors. Production and hosting by Elsevier B.V.
摘要：
Camellia oil obtained from Camellia oleifera seeds is rich in unsaturated fatty acids and unique flavors, and has become a rising high-quality edible vegetable oil in the world. However, honored as the "Oriental olive oil", Camellia oil was widely adulterated for the situation of high price and short supply. At present, the identification of adulterated plant edible oil is mainly based on the composition and content of fatty acids. Here, the fatty acid composition and content of the main vegetable edible oils were determined. It is found that the fatty acid composition and content are susceptible to the change of the origin, variety and climate of the raw materials, and adulterated oils could even be made extremely similar to Camellia oil by the target combination of fatty acid content, therefore it is difficult to accurately identify the adulteration of Camellia oil through the composition and content determination of fatty acids. Camellia oleifera DNA was used as the breakthrough point for adulteration identification. Basing on the EST library and transcriptome data of Camellia oleifera, 116 candidate specific DNAs were screened out by bioinformatics, then the optimized methods of trace DNA extraction in Camellia oil were established. Further, three specific Camellia oleifera DNAs that could only be PCR amplified using Camellia oil-extracted DNA as template were finally screened out, which were confirmed by exclusive PCR amplifications using DNAs of other edible oils as templates. One of the specific DNAs was used to make the concentration regression curves of trace DNA by qPCR (Quantitative real-time PCR). The computational model was successively established between the adulteration ratio and the Ct value of the qPCR by adulteration imitation of different proportions of Camellia oil. Finally, a complete identification system of Camellia oil adulteration was firstly established basing on the specific DNA of Camellia oleifera, and it may provide a new idea and method for identification of adulterated Camellia oil. (C) 2017 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.
摘要：
There are three key medicinal components (phellodendrine, berberine and palmatine) in the extracts of Phellodendron bark, as one of the fundamental herbs of traditional Chinese medicine. Different extraction methods and solvent combinations were investigated to obtain the optimal technologies for high-efficient extraction of these medicinal components. Results: The results showed that combined solvents have higher extracting effect of phellodendrine, berberine and palmatine than single solvent, and the effect of ultrasonic extraction is distinctly better than those of distillation and soxhlet extraction. Conclusion: The hydrochloric acid/methanol-ultrasonic extraction has the best effect for three medicinal components of fresh Phellodendron bark, providing an extraction yield of 103.12 mg/g berberine, 24.41 mg/g phellodendrine, 1.25 mg/g palmatine. (C) 2016 Production and hosting by Elsevier B.V. on behalf of King Saud University.
摘要：
近年来茶油掺假问题日益突出,传统理化方法难以进行有效鉴定,开发基于特征DNA的茶油掺假的高效鉴定技术十分必要。本研究探索不同方法提取茶油总DNA的提取效果,通过优化提取过程,确立了茶油总DNA最优的改良SDS提取方法。该法可从40 m L茶油中提取出足量用于PCR实验的茶油总DNA。进一步将获得的茶油总DNA作为模板进行特征DNA的PCR扩增验证,经DNA测序分析表明其PCR扩增适用性较好。
作者机构：
[贺舍予; Zeng, Yanling; Zeng, Xiaofeng; 杨亚; 谭晓风; 张党权] Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees (Central South University of Forestry and Technology), Ministry of Education, Changsha, China;[贺舍予] College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, China;[张党权; Zeng, Yanling; 谭晓风] Hunan Provincial Key Laboratory of Forestry Biotechnology, Changsha, China
通讯机构：
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees (Central South University of Forestry and Technology), Ministry of Education, Changsha, China
