Constituent diversity of ethanol extracts from pitaya
作者：
Ma, Jinghua;Mo, Wei;Zhang, Pangpan;Lai, Yong;Li, Ximei* ;...
期刊：
ASIA-PACIFIC JOURNAL OF CHEMICAL ENGINEERING ,2020年15(S1):e2478- ISSN：1932-2135
通讯作者：
Li, Ximei;Zhang, Dangquan
作者机构：
[Lai, Yong; Li, Ximei; Zhang, Dangquan; Zhang, Pangpan; Ma, Jinghua] Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Peoples R China.;[Mo, Wei] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.
通讯机构：
[Li, XM; Zhang, DQ] H;Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Peoples R China.
关键词：
bioctive constituents;ethanol extract;GC/MS;red pitaya;white pitaya
摘要：
Pitaya is rich in nutrition and unique in function and contains vegetable albumin, anthocyanins, rich vitamins, and water soluble dietary fiber, which are rare in fruits and vegetables. However, researches mostly focused on pitaya cultivation, biological characteristics, fresh-keeping, and lacked analyses about chemical composition of pitaya. Therefore, ethanol extraction and GC/MS were used to extract and identify the bioactive components in pulp and pericarp of red pitaya and white pitaya. The extracting results showed that percentages of ethanol extracts from pulp and pericarp of red pitaya were 14.5% and 12%, whereas those of white pitaya were 16% and 12%. The GC/MS results showed that different kinds of bioactive constituents were identified in fresh pericarp and pulp of red pitaya and white pitaya. In the four ethanol extracts, there are rich polysiloxane plant organosilicon with total contents of 10.09%, 24.21%, 25.77%, and 23.25%. Meanwhile, there are many heterocyclic substances, and the content of pericarp is much higher than that of pulp. The four ethanol extracts also contain some other bioactive ingredients, such as esters, hydrocarbons, alcohols, amides, halides, and fatty acids, which can be used as raw materials for biomedicine, advanced cosmetics, and skin care products. © 2020 Curtin University and John Wiley & Sons, Ltd.
语种：
英文
展开
Highly efficient regeneration and medicinal component determination of Phellodendron chinense Schneid
作者：
He, Hanjie;Qin, Jieming;Ma, Ziteng;Sun, Wenkai;Yan, Wende;...
期刊：
In Vitro Cellular & Developmental Biology - Plant ,2020年56(6):775-783 ISSN：1054-5476
通讯作者：
He, Gongxiu
作者机构：
[Sun, Wenkai; Ma, Ziteng; Qin, Jieming; He, Hanjie; Yan, Wende] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Natl Engn Lab Appl Technol Forestry & Ecol South, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.;[He, Gongxiu] Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Hunan, Peoples R China.;[Yang, Maoxun] Zhuhai Hopegenes Med & Pharmaceut Inst Co Ltd, Zhuhai Da Hengqin Sci & Technol Dev Co Ltd, Zhuhai 519000, Guangdong, Peoples R China.;[Zhang, Dangquan] Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Henan, Peoples R China.
通讯机构：
[He, Gongxiu] C;Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Hunan, Peoples R China.
关键词：
Phellodendron chinense Schneid;Regeneration system;Plant growth regulator;Medicinal plant
摘要：
Phellodendron chinense Schneid is an important Chinese herb with berberine and phellodendrine in stems and leaves, but with little information available on in vitro culture of this species. Disinfection of explants in 75% alcohol for 45 s, sterilization in 0.1% HgCl2 for 20 min, and submersion in 1.0 mol L-1 gibberellin3 (GA(3)) solution for 24 h was the optimal condition for seed germination. Murashige and Skoog's (MS) medium supplemented with 2.0 mg L-1 6-benzylaminopurine (6-BA) in combination with 1.5 mg L-1 1-naphthylacetic acid (NAA) was optimal for callus induction. MS medium supplemented with 2.0 mg L-1 6-BA was the appropriate medium for induction of adventitious shoots, and 1/2MS medium supplemented with 2.0 mg L-1 indole-3-butytric acid (IBA) and 0.5% active carbon was the optimal medium for root induction. The 15-d survival rate of regenerated plantlets after transplanting to basins containing perlite and peat moss (1:4) was greater than 80%, and the berberine and phellodendrine accumulation was lower in callus compared with regenerated plantlets. The establishment of highly efficient regeneration system provides technical support for genetic breeding of Phellodendron chinense Schneid.
语种：
英文
展开
西伯利亚杏仁粕营养物质测定与其氨基酸组成评价
作者：
徐克芹;朱绪春;张党权;乌云塔娜;赵罕
期刊：
经济林研究 ,2019年37(01):133-138 ISSN：1003-8981
作者机构：
中南林业科技大学 林学院,湖南 长沙 410004;中南林业科技大学 经济林培育与保护省部共建教育部重点实验室,湖南 长沙 410004;中南林业科技大学 湖南省林业生物技术重点实验室,湖南 长沙 410004;国家林业局泡桐研究开发中心 经济林种质创新与利用国家林业局重点实验室,河南 郑州 450003;国家林业局泡桐研究开发中心 经济林种质创新与利用国家林业局重点实验室,河南 郑州,450003
关键词：
杏仁粕;营养成分;氨基酸;含量与组成;测定分析
摘要：
为了探究西伯利亚杏仁粕的营养成分及其特点,以西伯利亚杏仁粕为试材,分别采用溶剂法、灼烧法、凯氏定氮法等方法对杏仁粕营养成分的含量与组成进行了测定与分析;采用氨基酸自动分析仪测定了杏仁粕中氨基酸的组成,并应用氨基酸比值系数法,以WHO/FAO氨基酸参考模式为评价标准,对其必需氨基酸的组成进行了评价。测定结果表明:西伯利亚杏仁粕中蛋白质的含量为648.00mg·g^-1,粗脂肪含量为54.00mg·g^-1,粗纤维含量为3.20mg·g^-1,灰分含量为44.00mg·g^-1。西伯利亚杏仁粕中含有17种氨基酸,氨基酸总含量为65.86%;西伯利亚杏仁粕中8种人体必需氨基酸的总含量为20.59%,其中谷氨酸含量最高,为8.70%,其必需氨基酸与非必需氨基酸的含量比为0.45;其药用氨基酸含量高达41.79%。杏仁粕中还含有丰富的Cu、Zn、Fe、Mn、K、Ca、Mg、P等微量元素和多种维生素,其中维生素E和维生素B2的含量分别为1.39×10^-2与0.38×10^-3mg·g^-1。其必需氨基酸的构成比例基本符合食品法典委员会(WHO/FAO)的标准,其氨基酸的比值系数评分(SRC)为76.51。研究结果表明:西伯利亚杏仁粕营养丰富,所含人体必需氨基酸的种类齐全,且配比均衡,是有利于人体氨基酸营养平衡的优质食品原料。
语种：
中文
展开
木荷人工林生物量及生产力研究
作者：
韦昌幸;张党权;王凤琴;严理;秦武明
期刊：
广西林业科学 ,2019年48(4):509-513 ISSN：1006-1126
作者机构：
[韦昌幸; 王凤琴] 广西国有高峰林场;中南林业科技大学林业生物技术湖南省重点实验室;广西大学林学院;[严理; 秦武明] 广西大学;[张党权] 中南林业科技大学
关键词：
生物量;生产力;木荷;人工林
摘要：
以广西国有高峰林场48年生木荷(Schima superba)人工林为研究对象,探讨其生物量及生产力。结果表明,木荷人工林单株生物量为81.61~576.12 kg;总生物量为281.79 t/hm^2,其中乔木层、林下层分别为268.71和13.08 t/hm^2,占比分别为95.36%和4.64%;乔木层生产力为7.77 t·hm^-2·a^-1,占林分总生产力(7.89 t·hm^-2·a^-1)的98.48%。
语种：
中文
展开
Effects of exogenous 6-BA and NAA on growth and contents of medicinal ingredient of Phellodendron chinense seedlings
作者：
He, Hanjie;Qin, Jieming;Cheng, Xuexiang;Xu, Keqin;Teng, Linzuo;...
期刊：
Saudi Journal of Biological Sciences ,2018年25(6):1189-1195 ISSN：1319-562X
通讯作者：
Zhang, Dangquan
作者机构：
[Zhang, Dangquan] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zhang, Dangquan] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.
关键词：
6-Benzylaminopurine;Antioxidase;Medicinal ingredient;Phellodendron chinense;alpha-naphthyacetic acid
摘要：
Using Phellodendron chinense seedlings as material, and treated with different concentrations of exogenous 6-Benzylaminopurine (6-BA) and alpha-naphthyacetic acid (NAA), then observed the growth status. Furthermore, we detected the contents of chlorophyll and soluble sugar, the activities of antioxidases by spectrophotometry, and determined the contents of secondary metabolite by high performance liquid chromatograph. The results showed that different concentrations of exogenous 6-BA increases the fresh weights and plant heights of Phellodendron chinense seedlings, and enhances the contents of chlorophyll and soluble sugar. NAA promoted growth, but deduced the contents of soluble sugar. Compared with control, culturing for 40 d, proper concentrations 6-BA enhanced the activity levels of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), proper concentrations NAA increased the activity levels of SOD and CAT, but decreased the levels of POD compared with CK. Suitable concentrations 6-BA enhanced contents of berberine, phellodendrine and palmatine in stems, proper concentrations NAA increased contents of berberine and phellodendrine, but deduced contents of palmatine compared with CK. Based on these results, we concluded that the exogenous 6-BA and NAA had key regulation on the growth and contents of medicinal ingredient of Phellodendron chinense seedlings. (C) 2017 The Authors. Production and hosting by Elsevier B.V.
语种：
英文
展开
Systematic characterization of volatile organic components and pyrolyzates from Camellia oleifera seed cake for developing high value-added products
作者：
Liu, Li;Cheng, Xuexiang;Zhao, Weiwei;Wang, Yunhao;Dong, Xiang;...
期刊：
Arabian Journal of Chemistry ,2018年11(6):802-814 ISSN：1878-5352
通讯作者：
Zhang, Dangquan;Peng, Wanxi
作者机构：
[Liu, Li; Peng, Wanxi; Zhang, Dangquan; Peng, WX; Cheng, Xuexiang; Zhao, Weiwei] Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Henan, Peoples R China.;[Liu, Li; Wang, Yunhao; Cheng, Xuexiang; Chen, Lili; Zhang, Dangquan; Dong, Xiang] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410117, Hunan, Peoples R China.
通讯机构：
[Zhang, DQ; Peng, WX] H;Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Henan, Peoples R China.
关键词：
Camellia oleifera seed cake (COSC);Volatile organic component (VOC);Pyrolyzates;Organic solvent extractive;Component characteristic
摘要：
Camellia oleifera seed cake (COSC), a byproduct during oil production of C. oleifera seeds, has been extremely abundant. However, due to the lack of systematic and in-depth analysis about the chemical composition of COSC, it is difficult to develop high value-added products, resulting in low processing efficiency or even directly abandoned. In this paper, the VOCs (Volatile organic components) characteristics of COSC and the variation rule of COSC groups before/after extraction were revealed, and the thermal loss law of COSC and the pyrolyzates characteristics at different temperatures were also explained. The main VOCs of ethanol extractive of COSC are alcohols, those of petroleum ether extractive are alkanes and organic acids, and those of benzene/ethanol extractive are esters. It is first reported here that rich 1,6,10-Dodecatrien-3-ol,3,7,11-trimethyl-, (E)-, namely nerolidol with wide use in cosmetics and biomedicine, exists in COSC extractives. In addition, bioactive VOCs such as β-caryophyllene, humulene and (E)-Atlantone were observed in three COSC extractives. The total content trend from high to low is petroleum ether extractive, ethanol extractive, benzene/ethanol extractive, indicating that petroleum ether extractive has the best developing prospects. The analytic results of FTIR further confirm that: (1) the COSC contains components including Si compounds, ethers, organic acids, esters and alcohols, (2) the four kinds of organic silicon detected are naturally occurring components in COSC, and (3) organic solvent extraction does not make compound groups of COSC significantly changed. There are four obvious stages in thermogravimetry treatment of COSC: the first (30–100 °C), the second (180–240 °C), the third (240–400 °C), and the fourth (400–567 °C), and the order of the mass loss is the third, the second, the fourth and the first. During thermogravimetry treatment, three critical turning points of temperature (240 °C, 400 °C and 567 °C) were observed, accompanied by significantly chemical changes such as macromolecule pyrolyzed into small volatile molecules. The four COSC extractive pyrolyzates at 300 °C, 450 °C, 600 °C and 750 °C have different content variation in the components, such as heterocyclic type with a high-low-high-low change, hydrocarbons and acids shown by the high-low-low-high process, and ketone and the alcohol with a low-high-low-high change. The 450 °C pyrolyzates of COSC residue after extraction are heterocyclic, ketone, phenolic more than esters, hydrocarbons, phosphide, acids, aldehydes, alcohols and acyl; the 600 °C pyrolyzates are heterocyclic, ketone, phenolic more than esters, ammonium, phosphide, acid, aldehydes and alcohols. Here, a large number of new components are produced in the pyrolysis treatment for COSC extractive and residue, providing a new approach for the high-grade application of COSC. © 2018
语种：
英文
展开
New method for effective identification of adulterated Camellia oil basing on Camellia oleifera-specific DNA
作者：
Cheng, Xuexiang;Yang, Tao;Wang, Yunhao;Zhou, Bingqian;Yan, Li;...
期刊：
Arabian Journal of Chemistry ,2018年11(6):815-826 ISSN：1878-5352
通讯作者：
Zhang, Dangquan
作者机构：
[Zhou, Bingqian; Cheng, Xuexiang; Zhang, Dangquan] Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Henan, Peoples R China.;[Teng, Linzuo; Wang, Yunhao; Cheng, Xuexiang; Chen, Lili; Zhang, Dangquan] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 412006, Hunan, Peoples R China.;[He, Yan; Yang, Tao; Yan, Li; Wang, Fangbin; Guo, Kunpeng] Hunan Inst Food Qual Supervis & Res, Changsha 410117, Hunan, Peoples R China.
通讯机构：
[Zhang, Dangquan] H;Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Henan, Peoples R China.
关键词：
Camellia oil;Specific DNA;Adulteration identification;Camellia oleifera;Fatty acids
摘要：
Camellia oil obtained from Camellia oleifera seeds is rich in unsaturated fatty acids and unique flavors, and has become a rising high-quality edible vegetable oil in the world. However, honored as the "Oriental olive oil", Camellia oil was widely adulterated for the situation of high price and short supply. At present, the identification of adulterated plant edible oil is mainly based on the composition and content of fatty acids. Here, the fatty acid composition and content of the main vegetable edible oils were determined. It is found that the fatty acid composition and content are susceptible to the change of the origin, variety and climate of the raw materials, and adulterated oils could even be made extremely similar to Camellia oil by the target combination of fatty acid content, therefore it is difficult to accurately identify the adulteration of Camellia oil through the composition and content determination of fatty acids. Camellia oleifera DNA was used as the breakthrough point for adulteration identification. Basing on the EST library and transcriptome data of Camellia oleifera, 116 candidate specific DNAs were screened out by bioinformatics, then the optimized methods of trace DNA extraction in Camellia oil were established. Further, three specific Camellia oleifera DNAs that could only be PCR amplified using Camellia oil-extracted DNA as template were finally screened out, which were confirmed by exclusive PCR amplifications using DNAs of other edible oils as templates. One of the specific DNAs was used to make the concentration regression curves of trace DNA by qPCR (Quantitative real-time PCR). The computational model was successively established between the adulteration ratio and the Ct value of the qPCR by adulteration imitation of different proportions of Camellia oil. Finally, a complete identification system of Camellia oil adulteration was firstly established basing on the specific DNA of Camellia oleifera, and it may provide a new idea and method for identification of adulterated Camellia oil. (C) 2017 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.
语种：
英文
展开
High-efficient extraction of principal medicinal components from fresh Phellodendron bark (cortex phellodendri)
作者：
Xu, Keqin;He, Gongxiu;Qin, Jieming;Cheng, Xuexiang;He, Hanjie;...
期刊：
Saudi Journal of Biological Sciences ,2018年25(4):811-815 ISSN：1319-562X
通讯作者：
Zhang, Dangquan;Peng, Wanxi
作者机构：
[Peng, Wanxi; Zhang, Dangquan; Peng, WX] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zhang, DQ; Peng, WX] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.
关键词：
Phellodendron;Cortex phellodendri;Extraction methods;Medicinal components
摘要：
There are three key medicinal components (phellodendrine, berberine and palmatine) in the extracts of Phellodendron bark, as one of the fundamental herbs of traditional Chinese medicine. Different extraction methods and solvent combinations were investigated to obtain the optimal technologies for high-efficient extraction of these medicinal components. Results: The results showed that combined solvents have higher extracting effect of phellodendrine, berberine and palmatine than single solvent, and the effect of ultrasonic extraction is distinctly better than those of distillation and soxhlet extraction. Conclusion: The hydrochloric acid/methanol-ultrasonic extraction has the best effect for three medicinal components of fresh Phellodendron bark, providing an extraction yield of 103.12 mg/g berberine, 24.41 mg/g phellodendrine, 1.25 mg/g palmatine. (C) 2016 Production and hosting by Elsevier B.V. on behalf of King Saud University.
语种：
英文
展开
39种桉树微卫星序列的遗传多样性分析
作者：
刘果;张党权;谢耀坚;陈鸿鹏;罗建中;...
期刊：
分子植物育种 ,2017年15(9):3767-3779 ISSN：1672-416X
作者机构：
国家林业局桉树研究开发中心, 湛江, 524022;中南林业科技大学, 林业生物技术湖南省重点实验室;[张党权] 经济林育种与栽培国家林业局重点实验室;[张党权] 经济林培育与保护湖南省省部共建教育部重点实验室, 长沙, 410004;[刘果; 谢耀坚; 陈鸿鹏; 罗建中; 吴志华] 国家林业局桉树研究开发中心, 湛江, 524022
关键词：
桉树;SSR分子标记;微卫星多态性;遗传多样性;亲缘关系
摘要：
为进一步研究桉树微卫星跨种的相似性及长度多态性,从基因组DNA遗传变异的角度探讨各个桉树种间的遗传多态性程度,本研究选用2个扩增结果稳定,微卫星完整的SSR位点进行PCR产物直接测序,从DNA序列的层次上对39种桉树进行遗传多样性分析和微卫星多态性分析。从SSR位点的层次上进行遗传多样性分析发现,2个SSR位点Nei's遗传多样性指数H_s平均为0.576,期望杂合度H_e平均为0.736,多态信息含量PIC平均为0.694,位点eSSR-GR124遗传多样性较高,位点gSSR-CA013序列相对更为保守。基于微卫星序列和侧翼保守序列的比对分析,微卫星重复次数的变化可能与属内各亚属间的分类相关性较大,微卫星序列的碱基替换可能与属间分类的相关性更大。由2个位点的综合序列进行相似性比较和系统发育树分析发现,位点eSSR-GR124和位点gSSR-CA013能够作为伞房属和桉属属间分类鉴定的依据。
语种：
中文
展开
600℃下油茶饼粕乙醇提取物与提取残渣热裂解产物的组分分析
作者：
张晓蕾;章怀云;张党权;刘欢;李碧霞;...
期刊：
经济林研究 ,2016年34(2):86-91 ISSN：1003-8981
作者机构：
中南林业科技大学经济林培育与保护省部共建教育部重点实验室;中南林业科技大学林业生物技术湖南省重点实验室;[李碧霞] 江西春源绿色食品有限公司;[章怀云; 刘欢; 张晓蕾; 莫薇; 张党权] 中南林业科技大学
会议名称：
中国林学会经济林分会2015年学术年会
会议时间：
2015-11-21
会议地点：
南京
会议论文集名称：
中国林学会经济林分会2015年学术年会论文集
关键词：
油茶饼粕;提取物;提取残渣;热裂解-气质联用
摘要：
油茶饼粕是油茶种子制备茶油后的副产物，含有多种生物活性成分。为给油茶饼粕资源的高效利用提供理论依据，采用索氏抽提方法，以乙醇为浸提溶剂，对冷冻干燥除水后的油茶饼粕进行浸提，对其提取物和提取残渣进行真空浓缩干燥处理，并在600℃的条件下分别进行热裂解，然后对热裂解产物的组分进行热裂解-气质联用（Py-GC/MS）分析。结果表明：油茶饼粕乙醇提取物的热裂解组分中含有杂环类、酮、酯、酚、烃类、醛等成分，而其提取残渣的热裂解组分中含有杂环类、酮、酸、酯、醚、酚、糖类、氨基酸、腈等成分；油茶饼粕乙醇提取物与提取残渣的热裂解产物中的部分组分可用作生物医药、香料、化妆品的生产原料。
语种：
中文
展开
6-BA和GA3对盐胁迫下红杆铁皮石斛幼苗生理生化影响
作者：
覃杰明;何含杰;张党权;陈丽莉;刘欢
期刊：
亚热带植物科学 ,2016年45(1):27-31 ISSN：1009-7791
作者机构：
中南林业科技大学,经济林培育与保护省部共建教育部重点实验室,林业生物技术湖南省重点实验室,湖南 长沙410018;[陈丽莉; 刘欢; 何含杰; 张党权; 覃杰明] 经济林培育与保护省部共建教育部重点实验室
关键词：
红杆铁皮石斛;生长调节剂;盐胁迫
摘要：
以红杆铁皮石斛Dendrobium officinale为材料,研究植物生长调节剂6-苄氨基腺嘌呤（6-BA）和赤霉素（GA3）对其幼苗在Na Cl胁迫下的生理生化影响。结果表明,盐胁迫下,低浓度6-BA和GA3处理均可不同程度地提高红杆铁皮石斛幼苗的茎生长率,抑制根的伸长,且GA3抑制效果大于6-BA;随着6-BA浓度增加,盐胁迫下红杆铁皮石斛叶片叶绿素含量降低,类胡萝卜素含量则呈先升后降的趋势,而较高浓度GA3（4.0 mg·L-1）处理同时提高叶绿素和类胡萝卜素含量;低浓度6-BA（2.0 mg·L-1）处理能提高红杆铁皮石斛叶片可溶性糖含量,相同浓度GA3处理则降低可溶性糖含量,但高浓度GA3处理的可溶性糖含量与对照无显著差异。因此,添加适当浓度的植物生长调节剂能提高光合色素及可溶性糖的合成与积累,从而缓解盐胁迫对红杆铁皮石斛植株的伤害。
语种：
中文
展开
茶油总DNA提取技术及扩增适用性
作者：
杨滔;程雪翔;陈丽莉;严礼;王芳斌;...
期刊：
广西林业科学 ,2016年45(4):381-385,396 ISSN：1006-1126
作者机构：
[杨滔; 郭焜鹏; 贺燕; 严礼; 王芳斌] 湖南省食品质量监督检验研究院;中南林业科技大学经济林培育与保护省部共建教育部重点实验室 林业生物技术湖南省重点实验室;[陈丽莉; 程雪翔; 张党权; 覃杰明] 中南林业科技大学
关键词：
茶油;DNA提取
摘要：
近年来茶油掺假问题日益突出,传统理化方法难以进行有效鉴定,开发基于特征DNA的茶油掺假的高效鉴定技术十分必要。本研究探索不同方法提取茶油总DNA的提取效果,通过优化提取过程,确立了茶油总DNA最优的改良SDS提取方法。该法可从40 m L茶油中提取出足量用于PCR实验的茶油总DNA。进一步将获得的茶油总DNA作为模板进行特征DNA的PCR扩增验证,经DNA测序分析表明其PCR扩增适用性较好。
语种：
中文
展开
Differential Gene Regulation of Lipid Synthesis in the Developing Seeds of Two Biodiesel Tree Species, Jatropha and Vernicia
作者：
Yang, Dan;Zhang, Huaiyun;Peng, Kuan;Chen, Lili;He, Hanjie;...
期刊：
International Journal of Agriculture and Biology ,2016年18(6):1143-1152 ISSN：1560-8530
通讯作者：
Zhang, Dangquan
作者机构：
[Zhang, Dangquan] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zhang, Dangquan] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.
关键词：
Biodiesel;Jatropha;Lipid synthesis;Transcriptomic analysis;Vernicia
摘要：
The fatty acid compositions of Jatropha oil and Vernicia oil are strikingly different, which leads to a great difference in combustion performance, low temperature performance and oxidation stability. A comparative transcriptomic study was made in Vernicia and Jatropha, with a focus on the gene regulation of differential oil accumulation process. Transcriptome sequencing was conducted with seeds at the initial- and fast- stage of oil accumulation from both. More than 24 billion bases of cDNA sequence were obtained, with 49,583 and 45,414 high-quality unigenes identified for Vernicia and Jatropha seeds, respectively. Multiple comparative transcriptome approaches revealed a number of species-specific fatty acid desaturases (FAD2, FADX, FAH12 etc.) contributing to their differentiated fatty acid compositions in seeds of Vernicia and Jatropha. Meanwhile, the results suggested that DGAT majorly regulates TAG synthesis than PDAT in Vernicia seed, and PDAT may have more important role regulating TAG synthesis in Jatropha seed than in Vernicia seed. It was also implied that specific oleosins involving in oil bodies may have member bias and may affect lipid contents in seeds of Vernicia and Jatropha, as some of which were 30-50 fold up-regulated (with their RPKM values over 10,000 at fast-stage). Some important factors were identified and can differentially regulate lipid pathways in seeds of Vernicia and Jatropha. © 2016 Friends Science Publishers.
语种：
英文
展开
不同磷源对油茶幼苗生长的影响
作者：
王金路;陈永忠;张党权;陈隆升;罗健
作者机构：
中南林业科技大学 经济林培育与保护教育部重点实验室,湖南 长沙 410004;湖南省林业科学院,湖南 长沙 410004
会议名称：
第三届中国林业学术大会
会议时间：
201309
会议地点：
福州
会议论文集名称：
第三届中国林业学术大会论文集
关键词：
油茶;磷肥;幼苗生长;磷含量
摘要：
研究采用盆栽试验,对油茶幼苗施用五种处理,分别是不施磷源的对照和施用K-P(KH2PO4)、AL-P(AlPO4)、Ca-P(Ca3(PO4)2)、Fe-P(FePO424H2O)等四种磷源.研究了不同磷源对油茶幼苗苗高、地径、主根长、侧根数、生物量、根冠比和根茎叶中氮磷钾含量的影响,结果表明:(1)K-P(KH2PO4)、AL-P(AlPO4)和Ca-P(Ca3(PO4)2)可以明显增加油茶幼苗的苗高、地径、生物量和主根长,减小侧根数和根冠比,而Fe-P(FePO424H2O作用不显著.(2)不同磷源处理都明显增加了油茶幼苗磷含量,K-P、Ca-P、AL-P和Fe-P处理的增加量分别为99.00％、90.90％、84.16％和48.51％.
语种：
中文
展开
6-BA和GA3对NaCl胁迫下铁皮石斛幼苗生长及可溶性糖含量的影响
作者：
覃杰明;张党权;何含杰
期刊：
北方园艺 ,2016年(10):144-147 ISSN：1001-0009
作者机构：
中南林业科技大学经济林培育与保护省部共建教育部重点实验室林业生物技术湖南省重点实验室
关键词：
铁皮石斛;盐胁迫;可溶性糖;色素
摘要：
以铁皮石斛为试材,在NaCl胁迫下,研究了6-苄氨基腺嘌呤（6-BA）和赤霉素（GA3）对铁皮石斛幼苗生长、色素及可溶性糖含量的影响。结果表明：2.0mg·L^-1 6-BA可以显著提高NaCl胁迫下铁皮石斛幼苗根的生长率,而4.0mg·L^-1 GA3则显著的提高其根的生长率,但二者均降低铁皮石斛茎的生长率;2.0mg·L^-1 6-BA则可以降低叶绿素b含量,而4.0mg·L^-16-BA则可以提高叶绿素的含量,且不同浓度6-BA对叶绿素a和类胡萝卜素的含量无显著性影响;2.0mg·L^-1 GA3均可以提高叶绿素a、叶绿素b和类胡萝卜素的含量,而6.0mg·L^-1 GA3则降低叶绿素b含量;4.0mg·L^-1 6-BA和6.0mg·L^-1 GA3均可以提高可溶性糖含量。因此,添加适当浓度的外源植物激素可以促进铁皮石斛中可溶性多糖的合成与积累。
语种：
中文
展开
油桐LEC1基因的cDNA克隆与序列分析
作者：
杨丹;章怀云;张党权;陈丽莉;何含杰;...
期刊：
经济林研究 ,2016年34(1):1-5 ISSN：1003-8981
作者机构：
中南林业科技大学经济林培育与保护省部共建教育部重点实验室,湖南长沙410004;中南林业科技大学林业生物技术湖南省重点实验室,湖南长沙410004;中南林业科技大学经济林培育与利用湖南省2011协同创新中心,湖南长沙410004;[陈丽莉; 章怀云; 杨丹; 何含杰; 陈容; 彭宽; 张党权] 中南林业科技大学
关键词：
油桐;cDNA克隆;序列分析
摘要：
为了解LEC1（Leafy Cotyledon 1）基因对油桐油脂合成过程的调控机理,以油桐种仁转录组数据库中基因的部分c DNA序列为基础,采用RT-PCR技术从油桐种子中克隆LEC1基因的全长c DNA,并对其进行序列分析。油桐LEC1基因全长c DNA为1 152 bp,编码区为729 bp（142~870）,编码243个氨基酸,5′端非编码区和3′端非编码区分别为141 bp和263 bp。该基因编码蛋白质的相对分子质量为27 025.4 Da,理论等电点为6.97;蛋白二级结构以不规则卷曲和α螺旋为主,是不具有跨膜结构的膜外蛋白。经对比发现,油桐LEC1具有典型的HAP3结构特点,在不保守的N端和C端中间有1个十分保守的结构域,与拟南芥、玉米、麻风树、黄连木等的LEC1氨基酸序列高度同源。
语种：
中文
展开
锥栗芽苗砧嫁接技术
作者：
谭露曼;袁德义;张党权;熊欢;刘冬明;...
期刊：
经济林研究 ,2016年34(3):153-157 ISSN：1003-8981
作者机构：
中南林业科技大学林学院;中南林业科技大学经济林育种与栽培国家林业局重点实验室;中南林业科技大学经济林培育与保护省部共建教育部重点实验室;中南林业科技大学经济林培育与利用湖南省2011协同创新中心;[朱周俊; 袁德义; 谭露曼; 熊欢; 张旭辉; 刘冬明; 张党权] 中南林业科技大学
关键词：
锥栗;芽苗砧嫁接;嫁接时期;砧木年龄;封口材料与封口方式
摘要：
为了提高锥栗芽苗砧嫁接成活率，以野生锥栗种子作为砧木，以‘华栗3号’当年生营养枝作为接穗，采用嫩枝劈接法，研究4个嫁接时期（4月20日、5月10日、6月1日、7月10日）、5种日龄砧木（20、30、45、60、90 d）和封口材料及封口方式（铝箔、嫁接膜封顶、嫁接膜不封顶）对锥栗芽苗砧嫁接成活率的影响。结果表明：封口材料与封口方式对嫁接成活率存在较大影响，不同的封口材料与封口方式处理的嫁接苗成活率从高到低依次为：嫁接膜封顶、铝箔、嫁接膜不封顶；6月1日为最佳嫁接时期，嫁接膜封顶处理的嫁接成活率达64.00%；砧木年龄过小（20 d）或过大（90 d）均对嫁接成活不利，当砧木日龄为45 d 时，嫁接成活率最高，为69.33%。
语种：
中文
展开
细胞分裂素6-BA对三裂叶野葛毛状根生长和抗氧化酶活性的影响
作者：
何含杰;黄小西;张党权;邓华凤
期刊：
北方园艺 ,2016年(14):156-159 ISSN：1001-0009
作者机构：
中南林业科技大学林业生物技术湖南省重点实验室/生物发育工程及新产品研发协同创新中心,湖南长沙,410018;杂交水稻国家重点实验室湖南杂交水稻研究中心,湖南长沙,410125;[何含杰; 黄小西; 张党权] 中南林业科技大学;[邓华凤] 湖南杂交水稻研究中心
关键词：
三裂叶野葛;毛状根;异黄酮化合物;可溶性蛋白质;抗氧化酶
摘要：
以三裂叶野葛毛状根为试材,研究了不同浓度6-BA对三裂叶野葛毛状根生长、异黄酮化合物及可溶性蛋白质含量和抗氧化酶活性的影响。结果表明:6-BA抑制三裂叶野葛毛状根的生长,降低其鲜样质量,且抑制作用与6-BA浓度成正比例关系,同时能提高毛状根培养物中异黄酮化合物的含量;但降低其毛状根培养物中可溶性蛋白质含量和超氧化物歧化酶（superoxide dismutase,SOD）活性水平,提高过氧化物酶（peroxidase,POD）活性水平,这表明6-BA可能通过调节三裂叶野葛毛状根的抗氧化酶活性进而调控其生长和异黄酮化合物的积累。
语种：
中文
展开
半定量RT-PCR方法检测FADX基因在油桐种子发育过程中的表达
作者：
杨丹;陈丽莉;张党权;彭宽;章怀云
期刊：
分子植物育种 ,2016年14(2):450-455 ISSN：1672-416X
作者机构：
[杨丹; 陈丽莉; 张党权; 彭宽; 章怀云] 中南林业科技大学, 林业生物技术湖南省重点实验室, 长沙, 410004
关键词：
油桐;半定量RT-PCR;表达分析
摘要：
桐酸合成酶基因FADX是油桐(Vernicia fordii)桐酸合成的关键基因,专一地表达于油桐正在发育的种子中。为研究其在油桐种子发育过程中的表达特点,筛选出甘油醛-3-磷酸脱氢酶基因GAPDH为内参基因,并以此检测FADX在油桐种仁中的表达。结果表明FADX在油桐果实发育早期处于低表达状态,至果实发育中期开始大量表达,这与油桐桐酸含量在果实发育过程中的变化规律是一致的,从一个方面证明FADX与油桐的油脂合成紧密相关,有助于油桐油脂合成与调控的研究。
语种：
中文
展开
红檵木腋芽高效快繁研究
作者：
陈容;张党权;郑玉娟;龚建平;陈丽莉;...
期刊：
经济林研究 ,2015年(4):96-101 ISSN：1003-8981
作者机构：
中南林业科技大学 经济林培育与保护省部共建教育部重点实验室，湖南长沙 410004;中南林业科技大学 林业生物技术湖南省重点实验室，湖南长沙 410004;中南林业科技大学 经济林培育与利用湖南省2011协同创新中心，湖南长沙 410004;湖南省澧县树大园林有限公司,湖南澧县,415513;[陈丽莉; 何含杰; 陈容; 周文化; 张党权; 郑玉娟; 覃杰明] 中南林业科技大学
关键词：
红檵木;腋芽;丛生芽;高效快繁
摘要：
为给红檵木组织培养与工厂化育苗提供技术参考，分别以红檵木2年生茎段和当年生茎段为外植体，通过优化红檵木腋芽的丛生芽诱导与增殖、生根与移栽方案，最终建立了以腋芽为基础的红檵木高效快繁体系。结果表明：红檵木茎段腋芽丛生芽诱导与增殖的最佳培养基配方分别为MS＋6-BA 1.3 mg/L＋NAA 0.2 mg/L和MS＋6-BA 1.2 mg/L＋IBA 0.07 mg/L＋Vc 5.0 mg/L；丛生芽生根的最佳培养方案为：选取高度在1.0～3.0 cm之间的植株，将其接种到3/4MS＋IBA 4 mg/L的培养基当中；生根组培苗的最佳移栽方案为：炼苗1周后，将其移栽到珍珠岩∶蛭石∶腐殖土＝1∶1∶3的基质中。
语种：
中文
展开
