近年来茶油掺假问题日益突出,传统理化方法难以进行有效鉴定,开发基于特征DNA的茶油掺假的高效鉴定技术十分必要。本研究探索不同方法提取茶油总DNA的提取效果,通过优化提取过程,确立了茶油总DNA最优的改良SDS提取方法。该法可从40 m L茶油中提取出足量用于PCR实验的茶油总DNA。进一步将获得的茶油总DNA作为模板进行特征DNA的PCR扩增验证,经DNA测序分析表明其PCR扩增适用性较好。
摘要(英文)：
In recent years, adulteration issues of Camellia oil have become increasingly prominent, while it＇ s too difficult to identify them effectively by traditional physical and chemical methods. It＇ s nec- essary to develop an efficient identification technology for Camellia oil adulteration based on its specific DNA. By optimizing the extraction process, the extraction effects of total DNA from Camellia oil by different extraction methods were analyzed. The optimal modified SDS extraction method for the total DNA of Camellia oil was established. ...
