Highly efficient regeneration and medicinal component determination of Phellodendron chinense Schneid

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期刊论文

作者：

He, Hanjie;Qin, Jieming;Ma, Ziteng;Sun, Wenkai;Yan, Wende;...

通讯作者：

He, Gongxiu

作者机构：

[Sun, Wenkai; Ma, Ziteng; Qin, Jieming; He, Hanjie; Yan, Wende] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Natl Engn Lab Appl Technol Forestry & Ecol South, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.

[He, Gongxiu] Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Hunan, Peoples R China.

[Yang, Maoxun] Zhuhai Hopegenes Med & Pharmaceut Inst Co Ltd, Zhuhai Da Hengqin Sci & Technol Dev Co Ltd, Zhuhai 519000, Guangdong, Peoples R China.

[Zhang, Dangquan] Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Henan, Peoples R China.

通讯机构：

[He, Gongxiu] C

Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Hunan, Peoples R China.

语种：

英文

关键词：

Phellodendron chinense Schneid;Regeneration system;Plant growth regulator;Medicinal plant

期刊：

In Vitro Cellular & Developmental Biology - Plant

ISSN：

1054-5476

年：

2020

卷：

期：

页码：

775-783

DOI：

10.1007/s11627-020-10080-1

基金类别：

This research was financially supported by the Key Research and Development Project of Hunan Province (No. 2018NK2041), Key Project of Hunan Education Department (No. 18A158; 19B590), and Changsha Science and Technology Project (No. kq1901144). These funding bodies had no influence on the design of the study, collection, analysis, interpretation of data, or the manuscript writing.

机构署名：

本校为第一且通讯机构

院系归属：

林学院

生命科学与技术学院

摘要：

Phellodendron chinense Schneid is an important Chinese herb with berberine and phellodendrine in stems and leaves, but with little information available on in vitro culture of this species. Disinfection of explants in 75% alcohol for 45 s, sterilization in 0.1% HgCl2 for 20 min, and submersion in 1.0 mol L-1 gibberellin3 (GA(3)) solution for 24 h was the optimal condition for seed germination. Murashige and Skoog's (MS) medium supplemented with 2.0 mg L-1 6-benzylaminopurine (6-BA) in combination with 1.5 mg L-1 1-naphthylacetic acid (NAA) was optimal for callus induction. MS medium supplement...

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Highly efficient regeneration and medicinal component determination of Phellodendron chinense Schneid

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