期刊论文

cDNA cloning and sequence analysis of LEC1 gene in Vernicia fordii

中文

经济林研究

1003-8981

2016

34

1

1-5

10.14067/j.cnki.1003-8981.2016.01.001

国家自然科学基金项目（31170640）；

本校为第一机构

为了解LEC1（Leafy Cotyledon 1）基因对油桐油脂合成过程的调控机理,以油桐种仁转录组数据库中基因的部分c DNA序列为基础,采用RT-PCR技术从油桐种子中克隆LEC1基因的全长c DNA,并对其进行序列分析。油桐LEC1基因全长c DNA为1 152 bp,编码区为729 bp（142~870）,编码243个氨基酸,5′端非编码区和3′端非编码区分别为141 bp和263 bp。该基因编码蛋白质的相对分子质量为27 025.4 Da,理论等电点为6.97;蛋白二级结构以不规则卷曲和α螺旋为主,是不具有跨膜结构的膜外蛋白。经对比发现,油桐LEC1具有典型的HAP3结构特点,在不保守的N端和C端中间有1个十分保守的结构域,与拟南芥、玉米、麻风树、黄连木...

In order to learn regulation mechanism of LEC1 gene（Leafy Cotyledon 1） to tung oil synthesis, the full-length c DNA of LEC1 gene was cloned from Vernicia fordii seeds by RT-PCR method on the basis of a part of c DNA sequences of the gene in transcription database of V. fordii kernel, and the sequences were analyzed. The full-length c DNA of LEC1 gene in V. fordii was 1 152 bp, ORF consisting of 729 bp（142-870） coded 243 amino acid, and 5′ UTR and 3′ UTR were 141 bp and 263 bp respectively. The relative molecular mass of coded protein by L...