摘要：
Liriodendron chinense (Hemsl.) Sarg (Chinese tulip tree) has a long history of utilization and plantation, but the chemical components of benzene/ethanol extractives of Liriodendron chinense (Hemsl.) Sarg leaves were unrevealed. The analytical result by method of GC/MS showed that the chemical components of benzene/ethanol extractives of freeze-dried Liriodendron chinense were identified as 55 constituent, and the main components are as: 2-Propenenitrile, 2-chloro- (13.75%), 1-Mercapto-2-heptadecanon (13.10 %), 1-Mercapto-2-heptadecanon (12.77%), Ethanol, 2-butoxy- (12.03%), 1-Docosanol (10.74%), Guanidine, (4-aminobutyl)- (5.05%), 5,10-Pentadecadiyn-1-ol, acetate (4.82%), 1,2,4-Butanetriol (3.13%), Thiophene, 2-ethyltetrahydro- (2.09%), 1-Eicosanol (2.00%), 1-Butanol, 3-methyl-, formate (1.60%), Butanoic acid (1.60%), .alpha.-D-Xylofuranoside, methyl 3,5-di-O-methyl- (1.30%), Phenol, 2,6-dimethoxy-4-(2-propenyl)- (1.29%),5.alpha.-Pregnane-12,20-dione (1.12%), Cyclopentanol (0.82%), etc. As the first report here, our result by GC/MS showed that the benzene-methanol extractive of freeze-dried leaves from Liriodendron chinense (Hemsl.) Sarg can be developed into top value-added materials of medicines and spicery.
摘要：
As a high stress resistance psammophyte with the characteristic of sand fixation and winterfat, Ceratoides lanata had been successfully introduced into many countries in recent years. The leaves and twigs of Ceratoides lanata in room temperature were used as the Driver and those treated by cold stress were used as Tester. Tester and Driver ds cDNA were prepared from their high quality mRNA which were purified from their total RNA. Tester and Driver cDNA were separately digested to obtain shorter, blunt-ended molecules by RasI. Then two Tester populations are created with different adaptors, while Driver cDNA has no adaptors. Differentially expressed genes were equalized and enriched by two round subtractive hybridizations using excess Driver population as compared with Tester population. The differentially expressed cDNAs were exponentially amplified by first round suppression PCR using the diluted hybridization product as template, and then the secondary PCR was performed using the first PCR product as template by nested primers to finally enrich the differentially expressed cDNAs, which consists of the SSH library of Ceratoides lanata. These cDNAs were inserted into vectors and 362 cDNA clones were obtained. The sequencing results showed that some cDNAs of cold-stressed Ceratoides lanata SSH library have relatively high homology with known stress resistance-related genes or proteins, and other cDNAs are new genes which are firstly reported here. Our result lays a foundation for the cDNA cloning of valuable genes including antifreeze genes, heat-resistant genes, drought-tolerant genes, alkali-salt-tolerant genes from Ceratoides lanata, and their transgenic application.
摘要：
The transformation of cDNA into plant is still not carried out directly by a universal transgenic vector, hence it is not convenient for the transgenic application of those functionimportant cDNAs. Herein we introduced a rapid, cost-effective PCR-based DNA synthesis method to construct a new universal plant overexpression vector for the direct inserting of target cDNA during transgenic application. The new engineered transgenic vector was based on the pCAMBIA vector backbone, which was inserted by five motifs, including a promoter, an enhancer, a Linker DNA fragment, a endoplasmic reticulum retention signal (KDEL), and a NOS-T DNA. The target cDNA can be directly inserted into the new universal vector to construct the workable transgenic plasmid according to the interesting need of transgenic users. The result reported here will promote the plant transgenic application of cDNAs.
摘要：
China has the rich bamboo resource, moreover, the species and yield of bamboo both are the first in the world. The extract of bamboo root was often used to cure some intractable diseases in the countryside of China, but the components of bamboo root extract is still unresolved. In order to expedite medicinal application of bamboo root, and to provide further scientific foundation for its top value-added development, pyrolysis- GC/MS technology was used to identify and analyze the top value-added biomedical and bioactive components of benzene/ethanol extract of Moso bamboo root. The extract was pyrolyzed at 750 °C in helium atmosphere, and then the pyrolyzate was analyzed by online-linked GC/MS. Relative content of each component was determined by area normalization. 87 compounds representing 98.31% were identified from 105 peaks. The result showed that the pyrolyzate of benzene/ethanol extract of Moso bamboo root is rich in biomedical components, and also contains some bioactive components which can be used as top value-added materials of high-grade spice, cosmetic, food, and chemical.
通讯机构：
[Zhang, Dang-Quan] C;Cent S Univ Forestry & Technol, State Forestry Adm, Key Lab Nonwood Forest Prod, Biotechnol Core Facil, Changsha 410004, Hunan, Peoples R China.
会议名称：
The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)
会议时间：
2009-06-11
会议地点：
北京
会议论文集名称：
The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)论文集
关键词：
Eucalyptus camaldulensis;pyrolysis- GC/MS (pyrolysis- gas chromatograph/ mass spectrometer);cold water eztractive;wastewater recovery;wood chips of papermaking;top value added
摘要：
Wood extractives could be suited directly for biomedicine. Eucalyptus granlla has become one of the most important plantation resources in fresh Eucalyptus granlla shavings direct current electric field, south China. Therefore, the were extracteded under the alternating electric field, electromagnetic field and soxhlet extracting, and the extractives were analyzed by ZETA and SIZER to find out the leaching rule of nano particles from Eucalyptus granlla wood. The results were following as: (1) The external electric and electromagnetic field-extracting approach could decrease the grouping number of leached particles from Eucalyptus granlla wood. And the most significant apoptosis effect on leached particles was induced under the alternating electric field. (2) The extraction mass arrived at the maximum value when extracting time is about 5h in the soxhletic-extracting approach. it is the best effect of electromagnetic field among the four extracting approaches.
摘要：
As the most important and special edible oil tree in China, tea-oil tree (Camellia oleifera) has the oil-yielded rate of about 55% from its kernel. The recent researches suggested that the oiliness of tea-oil should be one of the best plant oils, for tea-oil consists of ∼90% unsaturated fatty acids (∼80% oleic acid, ∼10% linoleic acid and linolenic acid). As the enzyme controlling the first-step desaturation during the biosynthesis of plant unsaturated fatty acids, plant SAD (Stearoyl-ACP desaturase) directly regulates the proportion of saturated fatty acids and unsaturated fatty acids by dehydrogenating saturated fatty acids bonded to ACP (Acyl carrier protein) to form oleic acids. However, recent researches on Camellia oleifera were mostly focused on the conventional planting and breeding and characteristic analysis of its products, and lacked those to find out the molecular mechanism of controlling the forming of high content of oleic acids. Therefore, we attempted to clone the full-length cDNA of Camellia oleifera SAD (CoSAD) gene. We successfully constructed a full-length cDNA library using the nearly matured seeds of elite cultivar Xianglin-4 planted widely in China, and then randomly selected hundreds of clones for sequencing from the 5′-end to construct a good representational EST library. Basing on the homological analyses of this EST library, we obtained 3 ESTs of CoSAD, and then obtained the full-length cDNA of CoSAD from the full-length cDNA library by PCR. The result of bioinformatic analyses including three-dimensional homological modeling on the CoSAD cDNA suggested that CoSAD displays high similarity to SADs of different plants, and is more advantageous than other plant SADs to catalyzing saturated fatty acids into oleic acids. This result could explain the higher content of oleic acids than those of other oil plants, presenting theoretical element for the application of CoSAD genes and genetic improvement on other oil plants.
摘要：
The rheological analysis on dynamic shear rate-viscosity relationship of tea-oil extracted from tea-oil tree seeds by supercritical extraction method was carried out at gradient temperatures and constant shear rate, respectively. The results show that at 20, 40, 60 and 80°C, once the shear rate increases gradually, the torque enlarges correspondingly, while the viscosity shows little difference. However, at the constant shear rate, the rising temperature results in a steady downtrend on tea-oil viscosity. This results reveal that tea-oil viscosity is not closely correlated with shear rate at constant temperature, yet negatively correlated with temperature at constant shear rate.
作者机构：
[彭万喜; 武书彬] State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China;[Zhang, Ning-Nan; 徐大平] Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China;[张宁南] The Key Laboratory of Non-Wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha 410004, China
通讯机构：
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, China
摘要：
The rapeseed oil extracted from the mature seeds was purified by refining processing, and the rheological characteristic analysis of the viscosity and dynamic shear rate at gradient temperatures was made. The result shows that at 20, 40, 60 and 80 °C respectively, when the shear rate gradually rises, the torque increases accordingly but its viscosity does not vary distinctly. The result suggests that when rapeseed oil is used as the raw of edible oils and industries, the working procedures at high temperature will not influence its rheological characteristic distinctly.
期刊：
PROCEEDINGS OF THE 2007 INTERNATIONAL CONFERENCE ON AGRICULTURE ENGINEERING,2007年:617-620
作者机构：
[Ma Qing-Zhi; Zhang Dang-Quan] Cent S Univ Forestry & Technol, Key Lab Non Wood Forest Prod State Forestry Adm, Changsha 410004, Peoples R China.
会议名称：
2007 International Conference on Agriculture Engineering(2007年农业工程国际会议)
会议时间：
2007-10-20
会议地点：
河北保定
会议论文集名称：
2007 International Conference on Agriculture Engineering(2007年农业工程国际会议)论文集
关键词：
Phyllostachys heterocycla var. pubescen;benzene/ethanol extractives;GC/MS
摘要：
The chemical components from benzene/ethanol extractives of fresh Phyllostachys heterocycla var. pubescen were separated and identified by GUMS after enriched. Relative content of each component was determined by area normalization. 42 compounds representing 98.29% of the extractives were identified. The main and abundant constituents were as: tetradecane,2,6,10-trimethyl-(7.81%), dodecane (7.39%), indole (7.02%), nonadecane (5.77%), pregn-4-ene-3,20-dione,11,21-dihydroxy-,(11a)- (5.46%), tridecane (5.46%), heptacosane (4.56%), 1,2-benzenedicarboxylic acid, diisooctyl ester (4.68%), quercetin 7.'3',4'-trimethoxy (3.69'%,), octadecane,6-methyl-(3.50%), tetradecane (3.39%), silicate anion tetramer (3.33%), tributyl phosphate (2.88%), dibutyl phthalate (5.22%), 1,2-benzenedicarboxylic acid,bis (2-methylpropyl) ester (2.86%), hexadecane (2.77%), ethyl linoleate (2.71%), pentadecane (2.64%), silicate anion tetramer (2.54%), heptacosane (2.10%), and so on. Most components of benzene/ethanol extractives of fresh Phyllostachys heterocycla var. pubescen are not only abundant and healthy to human, but used as bioenergy.
