作者机构：
[Zhou Guoying; Zhang Huaiyun; Li He] Cent S Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Hunan, Peoples R China.
通讯机构：
[Li He] C;Cent S Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Hunan, Peoples R China.
会议名称：
International Conference on Micro Nano Devices, Structure and Computing Systems
会议时间：
NOV 06-07, 2010
会议地点：
Int Sci & Engn Ctr, Singapore, SINGAPORE
会议主办单位：
Int Sci & Engn Ctr
会议论文集名称：
Advanced Materials Research
关键词：
Bamboo timber;Environmental protection type fiber material;Natural bamboo fiber;Research status
摘要：
The natural bamboo fiber is a green environmental protection type fiber material extracted from bamboo. Compared with other fibers, it has better permeability, unique resilience, transient water absorption, antibacterial activity, deodorant activity, and a strong lateral and longitudinal strength. This paper reviews the structure of natural bamboo fiber and its characteristics, physical morphology, chemical composition, hygroscopicity and moisture liberation, antibacterial activity, anti-ultraviolet function, spinning performance of the development and utilization status of bamboo fiber, which provides reference for the research and utilization of natural bamboo fiber.
摘要：
Aqueous extraction and alkaline (NaOH) hydrolysis method were comparatively studied in polysaccharides extraction from submerged-cultured Agaricus blazei mycelia. The results show that the polysaccharide content and the total extract rate from alkaline extraction method were obviously higher than those from the aqueous extraction method. Optimization results by the orthogonal matrix method on polysaccharide extraction from A. blazei show that the optimum alkaline concentration was 1.0 mol/L, hydrolysis temperature 60 degrees C, hydrolysis time 1 hour, and liquid: solid ratio 15. With the optimized condition, the polysaccharide extraction rate reached 11.93 +/- 0.68 %. The mycelial polysaccharide obtained with alkaline hydrolysis method was fractionated by the DEAE column chromatography. Five APS fractions were isolated in the polysaccharide sample with APS-1 as the main component.
作者机构：
[Zhou Guoying; Yang Lei; He Yuanhao; Li Lin; You Jia; Mei Jie] Biotechnology Core Facilities, College of Forestry, Central South University of Forestry and Technology, Changsha, 410004
会议名称：
International Conference on the Efforts in Response to Forest-Related Natural Disasters-the 1st Forest Science Frum(首届森林科学论坛——应对森林自然灾害国际学术会议)
会议时间：
2010-4-12
会议地点：
北京
会议主办单位：
中国林学会;中国气象学会;中国生态学学会;中国水土保持学会
会议论文集名称：
International Conference on the Efforts in Response to Forest-Related Natural Disasters-the 1st Forest Science Frum(首届森林科学论坛——应对森林自然灾害国际学术会议)论文集
关键词：
Camellia oleifera;Bacillus subtilis;colonization;biological control
摘要：
The complete coding sequence of Gibberella moniliformis phosphoenolpyruvate carboxykinase gene (PEPCK) was obtained by using Neurospora crassa PEPCK gene coding sequence (XM955062) as seeded sequence in silico cloning. Some characters of the PEPCK were analyzed and predicted by the tools of bioinformatics in the following aspects, including the composition of amino acid sequences, physical and chemical. These results showed that the full-length of PEPCK was 1948 bp and it contained a complete ORF (1671bp), encoded 556 amino acids, which are much conserved in ascomycetes. The calculated molecular weight of PEPCK was 61893.1 Da, theoretical p1 of 5.77 and was composed of 20 kinds of amino acid.
会议名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)
会议时间：
2010-06-18
会议地点：
成都
会议主办单位：
[Liu Jun-ang;Zhou Jian-hong;Zhou Guo-ying] Cent South Univ Forestry & Technol, Biotechnol Cores Facil, Changsha, Hunan, Peoples R China.
会议论文集名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)论文集
关键词：
Camellia oleifera anthracnose;pathogen;gene cloning;sequence analysis;entophytic bacteria;Biological control
摘要：
Filamentous fungal strains were isolated from the leaves and fruits of Camellia oleifera with the anthracnose disease. The pathogenecity was tested following Koch ' s postulates. Our analyses identified that all 18 isolated strains were pathogenic to Camellia oleifera. The molecular analysis of the internal transcribed spacer regions of the most virulent isolate confirmed that it belonged to Colletotrichum gloeosporioides. 165 strains were isolated from the leaves of Camellia oleifera, which were collected from Henyang, Liuyang, Changde etc in Hunan province. There were three entophytic bacteria strains Y13, S2, H18 against Colletotrichum gloeosporioides. And more, Strain Y13 was antagonistic against different Pathogenic fungi, Fusarium oxysporium, Thanatephorus cucumeris, Alternaria solan, PestalotiopsisStey, and its inhibition rates were 84.3%. 81.8%. 80.0% and 80.1%. The result showed that Strain Y13 had a wider antibacterial spectrum and the strong antagonistic activity. Our analyses and Strain Y13 study should help future identification, prevention and treatment of the Camellia oleifera anthracnose disease.
摘要：
Chitin deacetylase (CDA) catalyzes the conversion of chitin to chitosan by the deacetylation of N-acetyl-D-glucosamine residues. In this study, 9 strains of bacteria with chitin deacetylase activity were isolated from canal mud samples from the Xiangjiang River in Changsha, Hunan Province. Of these 9 strains, the Z7 strain had the highest chitin deacetylase activity, as determined by enzyme assay screening. Based on its culture, morphological, physiological characteristics and molecular identification, strain Z7 was identified as Bacillus amyloliquefaciens. Yeast extract at a concentration of 1.0% was an ideal nitrogen source for production of CDA (16.78 Eu/ml). Starch at 2% functioned best as the major carbon source for optimum production of CDA (17.84 Eu/ml). Magnesium sulfate at a concentration of 0.04% was an ideal inorganic salt for CDA production (17.45 Eu/ml). Ideal pH and temperature for optimum production of CDA by this strain were pH 6 and 37 degrees C.
摘要：
Pycnoporus cinnabarinus is a plant pathogen. It is common in many areas and is widely distributed throughout the world. Laccases of are some of the few oxidoreductases commercialized as industrial catalysts. In the present study, some characters of the amino acid sequence of P.cinnabarinus laccase (Lac1) were predicted and analyzed with the tools of bioinformatics. These results showed that the protein was composed of 20 kinds of amino acid; the theoretical pI of manganese peroxidase was 4.81 and the theoretical molecular weight of manganese peroxidase was 56292.0 Da; total number of atoms was 7806; the extinction coefficient was 58120 (280 nm). The N-terminal of the sequence considered was M (Met) and the estimated half-life was 30 hours (mammalian reticulocytes, in vitro). The instability index (II) was computed to be 34.50; this classifies the protein as stable. Aliphatic index was 82.64. Grand average of hydropathicity (GRAVY) was -0.063. There were 8 glycosylation sites, a signal peptide and conserved domains.
摘要：
Chitin deacetylase (CDA) is an enzyme that catalyzes the hydrolysis of acetamine groups of N-acetyl-D-glucosamine in chitin. Chitin deacetylase converting chitin to chitosan in fungal cell walls. A total of 208 strains was isolated from soil samples in the riverbank of Xiangjiang River and an island of Zhanjiang. 12 CDA producing strains was founded by color reaction that including three fungus, two bacterias and four actinomycetes. The highest CDA producing strain Z7 had been screened by enzyme activity assays. 16S gene of the strain was amplified and sequenced. The 16S gene sequence data were deposited in the GenBank nucleotide sequence database. The 16S gene sequences were aligned with the multiple - sequence alignment software Clustal S. Similarity values were calculated using the software MegAlign. Phylogenetic and molecular evolutionary analyses were conducted using MEGA by the neighbor - joining algorithms. Aphylogenetic tree was constructed by comparing with the published 16S gene regions of the related bacteria species. In the phylogenetic tree the overall similarity value between strain Z7 and Bacillus amyloliquefaciens are 99%. The wild strain was classfy as Bacillus amyloliquefaciens.
作者：
Li He;Zhou Guoying*;Guo Liang;Liu Yadi;Liu Junang
作者机构：
[Liu Junang; Zhou Guoying; Liu Yadi; Li He] Cent S Univ Forestry & Technol, Biotechnol Core Facil, Changsha 410004, Hunan, Peoples R China.
会议名称：
The Third International Conference on Modelling and Simulation(第三届国际建模、计算、仿真、优化及其应用学术会议 ICMS 2010)
会议时间：
2010-06-04
会议地点：
无锡
会议主办单位：
Jiangnan Univ
会议论文集名称：
The Third International Conference on Modelling and Simulation(第三届国际建模、计算、仿真、优化及其应用学术会议 ICMS 2010)论文集
关键词：
B. bassiana;chitinases;amino acid sequence;bioinformatics analysis
摘要：
Beauveria bassiana is one of the entomopathogenic fungi and has been successfully applied to biocontrol insect pest. B. bassiana mainly depends on cuticle degrading enzymes including proteases, chitinases and lipases to penetrate host's cuticle. In the present study, some characters of the amino acid sequence of B. bassiana chitinase were predicted and analyzed with the tools of bioinformatics. These results showed that the protein was composed of 20 kinds of amino acid, molecular weight was 36736.9 Da, theoretical p1 was 6.20 and total number of atoms was 5141. It was a hydrolyze and stable protein and contain a signal peptide. There were 7 glycosylation sites, 10 α-helix, 17 sheets and some irregular coiled peptides. It was glycosyl hydrolase, a member of family 18 type Ⅱ chitinases hydrolyze chitin.
摘要：
Camellia oleifera Abel (Theaceae) is a woody plant that is native to China. It is an important source of edible oil and is used in traditional medicine. It contains a triterpenoid saponin with high medical value that can be obtained from the seed cake residue (marc) after oil extraction. This saponin has prospects for wide applications. In this review, we summarize previous and current information regarding the chemical structure, physicochemical properties and biological activities of the saponin from the seed cake of C. oleifera and provide new insights for future research.
摘要：
Cordyceps militaris, a kind of caterpillar fungus, has therapeutic effects including immunoregulative, anticancer, antibacterial and antifungal effects. Aqueous extraction and alkaline (NaOH) hydrolysis method were comparatively studied in polysaccharides extraction from submerged-cultured C. militaris mycelia. The results indicate that the polysaccharide content and the total extract rate from alkaline extraction method were obviously higher than those from the aqueous extraction method. The extraction process parameters of alkaline extraction were further optimized using response surface methodology (RSM), and a mathematical model was then developed to show the effect of each extraction condition and their interactions on the polysaccharide extraction. The model predicted the optimum alkaline concentration was 1.6 mol/L, extraction temperature 56.7 square, extraction time 1.94, and the extraction rate was 11.57%. Furthermore, verification of the calculated optimum of the model was done with a condition representing this maximum point and obtaining a extraction rate of 11.12 %. The good correlation between predicted and measured values of these experiments justifies the validity of the response model and the existence of an optimum point.
摘要：
Gibberella moniliformis is a common disease in C. oleifera, and always results in significant economic losses. Phosphoenolpyruvate carboxykinase is a critical gluconeogenic enzyme and catalyzes the first committed step in the diversion of tricarboxylic acid cycle intermediates toward gluconeogenesis. According to the relative conservation of homologous gene, a bioinformatics strategy was applied to clone phosphoenolpyruvate carboxykinase gene of Gibberella moniliformis by blasting search of EST database with homologous gene cDNA of Neurospora crassa and identified. Some characters of the PEPCK that can encode amino acid were analyzed and predicted by the tools of bioinformatics in the following aspects, including the composition of amino acid sequences, physical and chemical, O-glycosylation site and tertiary structure of protein and function. These results showed that the full-length of PEPCK was 1962 bp and it contained a complete ORF (1671bp), encoded 556 amino acids, which are much conserved in ascomycetes. The calculated molecular weight of PEPCK was 61893.1 Da, theoretical pI of 5.77, 20 a-helix, 37 sheets, 7 glycosylation sites; it was a stable protein with active site ATP -binding site, metal-binding site and substrate-binding site.
摘要：
The study on molecular structure of Fusarium proliferatum clinically isolated can provide the basis for accurate diagnosis and treatment of F. proliferatum infection. The rDNA ITS gene of 6 strains that had been identified as Fusarium proliferatum based on morphological were cloned and sequenced. The molecule phylogenetic tree was constructed based on rDNA ITS sequences, and the 6 genus were divided into two groups. GroupI was F.proliferatum, including strains of CSUFT201, CSUFT204, CSUFT205. Group II was F. moniliforme, including CSUFT202,CSUFT203,CSUFT206. The results of this study showed that 3 strains of F. moniliforme were confirmed out of the 6 strains with the molecular methods.
