The complete coding sequence of Gibberella moniliformis phosphoenolpyruvate carboxykinase gene (PEPCK) was obtained by using Neurospora crassa PEPCK gene coding sequence (XM955062) as seeded sequence in silico cloning. Some characters of the PEPCK were analyzed and predicted by the tools of bioinformatics in the following aspects, including the composition of amino acid sequences, physical and chemical. These results showed that the full-length of PEPCK was 1948 bp and it contained a complete ORF (1671bp), encoded 556 amino acids, which are much conserved in ascomycetes. The calculated molecul...