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Screening and 16S rRNA Analysis of the Bacteria of Producing Chitin Deacetylase

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成果类型:
期刊论文、会议论文
作者:
Zhou Guo-ying*;He Yuan-hao;Zhang Huai-yun
通讯作者:
Zhou Guo-ying
作者机构:
[He Yuan-hao; Zhang Huai-yun; Zhou Guo-ying] Cent South Univ Forestry & Technol, Biotechnol Core Facil, Changsha, Hunan, Peoples R China.
通讯机构:
[Zhou Guo-ying] C
Cent South Univ Forestry & Technol, Biotechnol Core Facil, Changsha, Hunan, Peoples R China.
语种:
英文
关键词:
Chitin deacetylase;Screening;Sequences analysis;Bacillus amyloliquefaciens
期刊:
2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010)
ISSN:
2151-7614
年:
2010
页码:
1-4
会议名称:
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)
会议论文集名称:
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)论文集
会议时间:
2010-06-18
会议地点:
成都
会议主办单位:
[Zhou Guo-ying;He Yuan-hao;Zhang Huai-yun] Cent South Univ Forestry & Technol, Biotechnol Core Facil, Changsha, Hunan, Peoples R China.
会议赞助商:
IEEE生物医学工程协会<&wdkj&>武汉大学
出版地:
345 E 47TH ST, NEW YORK, NY 10017 USA
出版者:
IEEE
ISBN:
978-1-4244-4713-8
基金类别:
State Ministry of Forestry Key Project of the People's Republic of China. [2006-4-118]
机构署名:
本校为第一且通讯机构
院系归属:
生命科学与技术学院
摘要:
Chitin deacetylase (CDA) is an enzyme that catalyzes the hydrolysis of acetamine groups of N-acetyl-D-glucosamine in chitin. Chitin deacetylase converting chitin to chitosan in fungal cell walls. A total of 208 strains was isolated from soil samples in the riverbank of Xiangjiang River and an island of Zhanjiang. 12 CDA producing strains was founded by color reaction that including three fungus, two bacterias and four actinomycetes. The highest CDA producing strain Z7 had been screened by enzyme activity assays. 16S gene of the strain was amplified and sequenced. The 16S gene sequence data wer...

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