作者机构：
[Li He] Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Hunan, Peoples R China.;[Liu Junang; Zhou Guoying; Guo Liang] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.
通讯机构：
[Li He] C;Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Hunan, Peoples R China.
会议名称：
International Symposium on Manufacturing Systems Engineering (ISMSE 2011)
会议时间：
SEP 17-18, 2011
会议地点：
Hong Kong, PEOPLES R CHINA
会议主办单位：
[Li He] Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Peoples R China.^[Zhou Guoying;Guo Liang;Liu Junang] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.
会议论文集名称：
Advanced Materials Research
关键词：
L.deliciosus;GAPDH;Bioinformatics analysis
摘要：
Glyceraldehyde 3-phosphate dehydrogenase is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In the present study, some characters of the amino acid sequence of GAPDH of L.deliciosus were predicted and analyzed with the tools of bioinformatics. These results showed that the protein was composed of 20 kinds of amino acid; the theoretical pI of GAPDH was 7.08 and the theoretical molecular weight of GAPDH was 26165.9 Da; the total number of atoms was 3714. It was a stable protein. There were 7 glycosylation sites and it was a tetrameric NAD-binding enzyme involved in glycolysis and glyconeogenesis. N-terminal domain is a Rossmann NAD (P) binding fold. C-terminal domain is a mixed alpha/antiparallel beta fold.
摘要：
Plant extracts were efficient, safe bio-control stuff. In this paper we used two kinds of plant extracts, Eugenol and Baicalein, to against Colletotrichum gloeosporioides and Agaricodochium camellia by growth rate method. The results show that the inhibition effects of eugenol and baicalein were increasing as the rising concentration form 0 to 200ug/ml. The EC50 of eugenol against Colletotrichum gloeosporioides and Agaricodochium camellia were 30.37ug/ml and 51.55ug/ml respectively, the EC50 of baicalein against Colletotrichum gloeosporioides and Agaricodochium camellia were 61.26ug/ml and 49.83ug/ml respectively. The synergistic interaction values of the compounded agents with different volume ratio against Colletotrichum gloeosporioides and Agaricodochium camellia were between 0.5 and 1.5. When the volume ratio of eugenol and baicalein were 1:1 ,the prevention and treatment effects of compounded agent and carbendazim with were the same, up to 60%.
作者：
LI He;ZHOU Guo-ying*;XU Jian-ping;LIU Jun-ang;ZHANG Huai-yun;...
期刊：
Journal of Medicinal Plants Research,2012年6(2):171-176 ISSN：1996-0875
通讯作者：
ZHOU Guo-ying
作者机构：
1Hunan Forestry Biotechnology Key Laboratories, Central South University of Forestry and Technology, Changsha Hunan 410004, China. 2College of Forestry, Biotechnology Core Facilities, Central South University of Forestry and Technology, Changsha Hunan 410004, China.
摘要：
Ginkgo is a genus of highly unusual non-flowering plants with one extant species. The Ginkgoaceae is a family of gymnosperms which appeared during the Mesozoic Era, of which the only extant representative is Ginkgo biloba, which is for this reason sometimes regarded as a living fossil. Extracts of Ginkgo contain flavonoid glycosides and terpenoids (ginkgolides, bilobalides) and have been used pharmaceutically. Polysaccharides of G. biloba can reduce blood sugar, immune regulation, antioxidation, anti-inflammatory effects and play an important role in anti-tumor effects. At present, the method of water extraction and alcohol precipitation was generally used for the extraction of polysaccharides from G. biloba. The de-protein methods of polysaccharides were Savag’s and trichloroacetic acid method. The extracted polysaccharides were generally a mixture and usually use a combination of two or more methods to separate from proteins and other contaminants to yield pure polysaccharides. The commonly used methods for purification were the step-by-step precipitation, salting-out and column chromatography method. Polysaccharides of G. biloba were polymers of glucose, rhamnose, arabinose, mannose, galactose and xylose. In this review, we summarized previous and current researches on polysaccharides from G. biloba and provide new insights for future work on polysaccharide of this specie.
摘要：
This experiment isolated a series of endophytic bacteria strains form the healthy Camellia leaves. And the strain L32 had significant inhibitory effect to Camellia soft rot disease pathogen. The strain colonies in PDA medium was circular, surface drape, colony propagation rate was faster, ivory; in the early stage of growth the colony edge was neat; and later stage of growth it became corrugated, the centre of colony was uplift and opaque. Carry on the physiological and biochemical experiments and molecular identification, it was judged that the strain L32 had high relationship with the Bacillus subtilis.
关键词：
Control method;Pathogenic mechanism;Pathogeny;Pine;Pine wilt disease
摘要：
The pine is an important tree species in China, while the pine wilt disease is a devastating disease of pine treesPine wood nematode(Bursaphelechus xylophilus Steiner & Buhere, 1934 Nickle, 1981) is a
摘要：
Chinese fir (Cunninghamia Lanceolata) is a unique fast growing merchantable timber species in China with good materials, and is a major fast growing timber species in provinces of southern China. With the increase in Chinese fir plantation areas and the improvement in the degree of pure forests, the diseases of Chinese fir are increasing and their damages are getting worse, which have caused great losses and affected the sustainable, rapid and healthy development of forestry in China. This article gives a description of pathogenies, symptoms and occurrence regularities of four important diseases of Chinese fir, including Glomerella cingulata, Pestalotia Sp., Pseudomonas cunninghamiae and Chlorosis, and puts forward the corresponding control measures of these diseases, which provides an important basis for disease control of Chinese fir plantations.
期刊：
African Journal of Biotechnology,2011年10(30):5719-5724 ISSN：1684-5315
通讯作者：
Li Lin
作者机构：
[Liu Jun-Ang; Li Lin; Zhou Guo-Ying] Cent S Univ Forestry & Technol, Changsha 410004, Hunan, Peoples R China.;[Liu Jun-Ang; Li Lin; Zhou Guo-Ying; Li He] Cent S Univ Forestry & Technol, Coll Forestry, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Li Lin] C;Cent S Univ Forestry & Technol, Changsha 410004, Hunan, Peoples R China.
关键词：
Chinese fir;Mycorrhizal fungi;Mycorrhizal inocula;Vesicle-abuscular
摘要：
Chinese fir is one of the most important commercial timber species in south China. Mycorrhizal fungi has been applied in many trees and showed important effect on enhancing the productive forces and the soil activities, but there is little report about the mycorrhizal fungi on Chinese fir. So this paper investigated the mycorrhizal fungi resources of Chinese fir in Hunan province and analyzed the relation of community with the environment factors. The results showed that, abuscular mycorrhizal fungi (AMF) existed universally in the roots of Chinese fir. 75 specimen of AMF spores were isolated by wet-sieved method from this collection of the rhizosphere soils. They were identified by morphologic method to 4 genera and 9 species of AMF, which 5 species belong to Glomus, 1 of Gigaspora, 2 of Acaulospora and 1 of Scutellospora. In the isolated AMF, the importance value of Glomus mosseae, Glomus intraradices and Acaulospora laevis were more than 50% and the spore number of them was significantly higher than other species. These 3 species of AMF were considered as the dominate species in rhizosphere soil of Chinese fir in Hunan province. This research also indicated that, mycorrhiza forming was associated with the environment factors. The AMF spores density has the extremely remarkable correlational dependence with vegetation quantity in Chinese Fir forest (r2=0.943, p < 0.05). Mycorrhiza distribution also associated with seasons and forest age; the most active season of mycorrhiza was between spring and summer and the natural AMF infection rate was increased along with the increasing of forest age. The results will not only provide the theory basis for exploitation and using of mycorrhizal resource, but also enhance the survival rate of seedling and prevent the soil degradation and conserve of soil against erosion.Key words: Mycorrhizal fungi, vesicle-abuscular, Chinese fir, mycorrhizal inocula.
摘要：
To screen stimulators for cell growth and polysaccharides production of caterpillar-shaped Chinese medicinal mushroom, Cordyceps sinensis, the fungus was inoculated into the submerged culture media with and without supplementation of several fatty acids and ether extracts of Chinese medicinal insects. The results showed that palmitic acid at 1.0 g/l lead to significant increases in both biomass and extracellular polysaccharides (EPS) concentration (C) from 8.26 ± 0.34 to 11.10 ± 0.39 g/l and 353.1 ± 11.5 to 431.2 ± 13.8 mg/l, respectively. In addition, ether extract of medicinal insect, Eupolyphaga sinensis at 1.5 g/l was also of advantage to biomass and EPS production. There were no new components in the EPS obtained by the addition of palmitic acid.
关键词：
Ectomycorrhizal fungi;Pinus massoniana and Chinese Fir Plantation;Screening;Superior strains
摘要：
The ectomycorrhizal fungi resource of Pinus massoniana and Chinese Fir forest had been investigated in the places of Hunan and YunNan Province. There were 17 species belong to 6 families and 9 genera. All the collected ectomycorrhizal fungi were separated by tissue isolation method, and 12 strains were obtained, which belong to 7 genera of 4 families. The preliminary selection results showed that there were 6 strains presented the better culture characteristics, which were Seleroderma polyrhizum Pers., Scleroderma cepa Pers., Calvatia craniiformis(Schw.)Fr., Lactarius hatsudake Tanaka, Lactarius deliciosus(Fr)Gray., Russula puellaris Fr.. Then these 6 strains were cultivated between every two among them, the results showed that the group of LS3/RHG1 was the optimal combination, the next were the groups of LGD-2/La-HZ3 and LS3/La-HZ3. The groups of A3/ La8 showed antagonism to each other, so it was not suitable to be combined for inoculation. These research results will provide the important technical support for expanding propagation of ECM fungi and mycorrhizal technology application in Pinus massoniana and Chinese Fir plantation.
作者机构：
[Zhou Guoying; Zhang Huaiyun; Li He] Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha 410004, Hunan, Peoples R China.;[Liu Yadi; Liu Wenjin] Cent South Univ Forestry & Technol, Coll Furniture & Art Design, Changsha, Peoples R China.
通讯机构：
[Li He] C;Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha 410004, Hunan, Peoples R China.
会议名称：
International Conference on Micro Nano Devices, Structure and Computing Systems
会议时间：
NOV 06-07, 2010
会议地点：
Int Sci & Engn Ctr, Singapore, SINGAPORE
会议主办单位：
Int Sci & Engn Ctr
会议论文集名称：
Advanced Materials Research
关键词：
Bamboo fiber;Bleach;Crude fiber;Pretreatment
摘要：
Bamboo fiber is a new natural green environmental protection fiber material with good characteristics of good permeability, unique elasticity, transient absorption, antibacterial, odor resistance and stronger vertical and horizontal intensity. Bamboo pretreatment can improve the biological degumming efficiency of bamboo lignin, reduce microbial degumming time, and reduce production costs. The results show that the removal efficiency of pectin, hemicelluloses and lignin are better after the pretreatment of bamboo mercerization. Through orthogonal experiments, it can be got the optimum combination of bamboo fiber alkali cooking process is A2-B2-C2-D1, namely: concentration of NaOH is 6.0g / L, its time is 60min, temperature 100 °C, and bath ratio 1:20. In these four factors, the greatest impact on the removal of lignin is temperature, followed by is degumming time, then the concentration of alkali, and the bath ratio is the smallest. The best conditions of bamboo fiber bleaching is: hydrogen peroxide 25 ~ 35g / L, temperature 30 ~ 40 °C, treatment 45 ~ 60min. The process of the pretreatment of bamboo is: clip → Roll → high-pressure steaming → roll→ immersion separation → alkali leaching treatment→ scouring → rolling combing →washing→ drying →crude fiber → bleaching.
摘要：
Camellia anthrax disease is the main disease in producing areas of China. It occurred widespread, caused fruit and flowers dropped seriously, resulting in huge economic losses. In this paper, we separated pathogen with organizational separation method from the diseased leaves and fruit which were infected by Camellia anthrax. Then we proved, by Koch rule, it was pathogen which caused Camellia anthrax disease. According to their morphological characteristics, we identified that it was Colletotrichum gloeosporioides. From the Camellia healthy leaves, we isolated totally 125 strains endophytic fungi. Through the primary screening, 13 strains were detected had inhibited on Colletotrichum gloeosporioides. After the secondary screening, we obtained three strong antagonistic fungi, numbered Cd7, Sy3, Hy19, the inhibition rates separately were 60.3%, 59.7%, 57.8% on the PAD flat.
摘要：
The basidiomycete Craterellus tubaeformis (Fries) Quélet is an important widespread ectomycorrhizal basidio-mycete found in the Northern Hemisphere. In this study, 12 samples of C. tubaeformis from North America and Europe were analyzed using internal transcribed spacer (ITS) sequences to reveal the correlation between ITS genotypes and geographic locations and to provide molecular evidence for the identification of C. tubaeformis from different habitats in North America and Europe. The analyses identified abundant sequence variations within C. tubaeformis. The length of the ITS region varied from 571 to 640 bp. The proportion of variable sites was 17.6%, and the proportion of parsimony information sites was 16.7%. Phylogenetic analysis showed some correlations between the ITS genotypes and geographic locations of C. tubaeformis; however, some discrepancies between geographical location and affinity were also found. The results indicated that C. tubaeformis from different habitats in North America and Europe underwent genetic drifting and evolved into 2 different species. nrDNA ITS could be a good markers for distinguishing among C. tubaeformis from different habitats, but rational affinity should be determined by associating the available ITS data with other information sources.
摘要：
Lactarius hatsudake is a type of ectomycorrhizal fungus that significantly influences the growth of pine trees. It is widely prevalent in Asian countries and has a high economic value. Artificial cultivation of this fungus has not been achieved as yet; therefore, excessive manual harvesting may cause serious damages to the site of its production. In this study, we analyzed 41 samples of L. hatsudake from south China using internal transcribed spacer (ITS) sequences. By comparing the differences among ITS sequences to identify the haplotype diversity within each population, the relationships among local populations, the relationship between the level of genetic differentiation and geographical separation, and the contributions of local and regional geographical separations to the overall ITS haplotype variation were analyzed. Genetic analysis indicates that ITS sequences obtained from these 41 L. hatsudake samples could be identified as 18 haplotypes, of which 13 haplotypes were contained in only a single sample, whereas the remaining sequence types all were contained in two or more samples. The most common sequence type, haplotype 6, was found in 16 samples and was distributed across nearly every region. The Mantel test demonstrated that there is no significant linear relationship between geographical distance and the FST value of genetic difference. Results of this research illustrates that there exists a certain degree of genetic intermixing among natural populations of L. hatsudake. From the group genetic analysis, it appears that there exists genetic differentiation of lower frequencies in natural populations of L. hatsudake; however, the linear relationship between the degree of genetic differentiation and geographical distance is not distinctly apparent.
摘要：
Tea oil comes from unique woody oil trees in South China and is one of the four woody edible oils in the world. Tea oil is the edible oil obtained by squeezing mature seeds of oil tea. The unsaturated fatty acid content in tea oil can reach as much as 90%, which is the highest amount so far for unsaturated fatty acid in edible oil. The content of catechin in oil tea is about 80%, which have good whitening and anti-oxidation effects. Tea polyphenol is a natural antioxidant with significant antioxidation, which can eliminate free radicals, protect cell membrane structures, etc. Camellia saponin has many functions: Anti-permeability, anti-inflammatory, analgesic, anticancer, etc. Squalene is a kind of polyphenolic active composition with good oxygen-enriched capacity in oil tea. This paper summarizes the fatty acid composition and some functional components of tea oil, and its functions of anti-tumor, lipid-lowering, liver and heart protection, antisepsis and anti-inflammation, coronary heart disease prevention, delay of atherosclerosis, anti-oxidation, and regulation of the immune function. This paper aims to lay the foundation for further study and use of the medicine and health functions of tea oil.
