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Identification of a chitin deacetylase producing bacteria isolated from soil and its fermentation optimization

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成果类型:
期刊论文
作者:
Zhou, Guoying*;Zhang, Huaiyun;He, Yuanhao;He, Li
通讯作者:
Zhou, Guoying
作者机构:
[Zhang, Huaiyun; Zhou, Guoying; He, Yuanhao; He, Li] Cent S Univ Forestry & Technol, Biotechnol Core Facil, Changsha 410004, Hunan, Peoples R China.
[Zhang, Huaiyun; Zhou, Guoying; He, Yuanhao; He, Li] Cent S Univ Forestry & Technol, Coll Forestry, Changsha 410004, Hunan, Peoples R China.
通讯机构:
[Zhou, Guoying] C
Cent S Univ Forestry & Technol, Biotechnol Core Facil, Changsha 410004, Hunan, Peoples R China.
语种:
英文
关键词:
Chitin deacetylase;Chitosan;Bacillus amyloliquefaciens
期刊:
African Journal of Microbiology Research
ISSN:
1996-0808
年:
2010
卷:
4
期:
23
页码:
2597-2603
基金类别:
International Advanced Forestry Science and Technology Project Imported by State Forestry Administration [2006-4-118]
机构署名:
本校为第一且通讯机构
院系归属:
林学院
生命科学与技术学院
摘要:
Chitin deacetylase (CDA) catalyzes the conversion of chitin to chitosan by the deacetylation of N-acetyl-D-glucosamine residues. In this study, 9 strains of bacteria with chitin deacetylase activity were isolated from canal mud samples from the Xiangjiang River in Changsha, Hunan Province. Of these 9 strains, the Z7 strain had the highest chitin deacetylase activity, as determined by enzyme assay screening. Based on its culture, morphological, physiological characteristics and molecular identification, strain Z7 was identified as Bacillus amyloliquefaciens. Yeast extract at a concentration of ...

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