摘要：
Two new taxa are described from subtropical areas of China: Mycena chlorocyanea sp. nov. in sect. Viscipelles, and Mycena flosoides sp. nov. in sect. Polyadelphia. Photographs of basidiomata and morphological features, and relevant illustrations of these species are presented in this paper. Morphological characters were compared with related Mycena species, and phylogenetic analyses were conducted using internal transcribed spacer and the large subunit regions of nuclear ribosomal RNA gene sequences with Bayesian Inference and Maximum Likelihood. The phylogenetic trees showed that the two new taxa can be distinguished from phenotypically similar and phylogenetically related species.
作者机构：
[Shen, Ai-rong; Liu, Li-na; Tan, Zhu-ming; Shen, Bao-ming; Tan, Yun] Hunan Acad Forestry, Changsha, Peoples R China.;[Liu, Li-na; Zhou, Guo-ying] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha, Peoples R China.
通讯机构：
[Guo-ying Zhou] F;[Zhu-ming Tan] H;Faculty of Life Science and Technology, Central South University of Forestry and Technology, Changsha, PR China<&wdkj&>Hunan Academy of Forestry, Changsha, PR China
摘要：
Mycena subpiligera, a new taxon in sect. Fragilipedes that can strongly enhance the germination efficiency of Gastrodia elata seeds, was discovered in subtropical areas of China. As revealed by a morphological comparison with related Mycena species as well as maximum likelihood (ML) and Bayesian phylogenetic analyses based on sequences of the internal transcribed spacer (ITS) and the large subunit (LSU) regions of nuclear ribosomal RNA, the new taxon can be distinguished from phenotypically similar and phylogenetically related species. Optimal cultural conditions for M. subpiligera basidiomata are reported, and the germination rate of the new species is compared with that of M. citrinomarginata.
摘要：
采用十字交叉法测定CF17发酵液的抑菌谱,并通过离心、旋转蒸发浓缩、丙酮沉淀、固相萃取以及半制备高效液相色谱法对抑菌物质进行分离纯化和鉴定.结果表明,CF17发酵液对油茶炭疽病、油茶叶枯病、油茶软腐病、杉木炭疽病、核桃壳梭孢、降香黄檀炭疽病、檀香炭疽病等的病原菌均有抑菌效果.50%丙酮分离纯化效果优于乙醇沉淀法与乙酸乙酯沉淀法;在固相萃取中,活性物质主要集中在60%乙腈梯度洗柱样品中,最后经HPLC分离收集到一个有明显抑菌圈的样品.经质谱鉴定抑菌成分为Tetramycin A.
摘要：
Triterpene acids (TAs) are the major bioactive constituents in the medicinal fungus Ganoderma lingzhi. However, fermentative production of TAs has not been optimized for commercial use, and whether the TAs isolated from G. lingzhi submerged culture mycelia possess antitumor activity needs to be further proven. In this study, enhanced TA yield and productivity were attained with G. lingzhi using response surface methodology. The interactions of three variables were studied using a Box-Benhnken design, namely initial pH, dissolved oxygen (DO) and fermentation temperature. The optimum conditions were an initial pH of 5.9, 20.0% DO and 28.6 °C. These conditions resulted in a TA yield of 308.1 mg/L in a 5-L stirred bioreactor. Furthermore, the optimized conditions were then successfully scaled up to a production scale of 200 L, and maximum TA production and productivity of 295.3 mg/L and 49.2 mg/L/day were achieved, which represented 80.9% and 111.5% increases, respectively, compared with the non-optimized conditions. Additionally, the triterpene acid extract (TAE) from G. lingzhi mycelia was found to be cytotoxic to the SMMC-7721 and SW620 cell lines in vitro, and the TAE exhibited dose-dependent antitumor activity against the solid tumor sarcoma 180 in vivo. Chemical analysis revealed that the key active triterpene compounds, ganoderic acid T and ganoderic acid Me, predominated in the extract.
作者机构：
[刘高强; 王晓玲; Zhu, Chaoyang] College of Life Science and Technology, Central South University of Forestry &, Technology, Changsha, China;[王晓玲; 刘高强; Zhu, Chaoyang; 周国英] Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, China
通讯机构：
College of Life Science and Technology, Central South University of Forestry & Technology, Changsha, China
会议论文集名称：
International Workshop on Earth Observation and Remote Sensing Applications
关键词：
Chinese fir;disease stress;estimation;Hyperspectral;pigment content
摘要：
Using hyperspectral technology to test canopy of the fir which was damaged by the anthracnose, exploring and built the hyper-spectral estimation models of the pigment content in canopy of Chinese fir under the disease stress, the results will promote the application of the hyper-spectral remote sensing-technology in the forest pest and disease monitoring. From May to July in 2012, You Xian, Hunan Province, we investigated the anthracnose of Chinese fir, and measured the canopy pigment content and the spectral reflectance of the Chinese fir which was damaged by the anthracnose. The correlation between pigment contents of fir leaves with spectra reflectance, the first derivative of reflectance and spectral characteristic parameters were analyzed respectively. The result showed that the visible light and near-infrared region were the sensitive region which were reflected and absorbed by the pigment in disease of Chinese fir; pigment content had the highest correlation with the first order differential spectra in red edge (695-754 nm), and the correlation coefficient of single band first order differential spectrum in 741 nm was the largest. And it also showed that the accuracy of Chla+b, Chla and Chlb contents was the highest estimated by power function model which used the difference vegetation index DVI [FD587, FD741] as the variable, the relative errors were less than 15%, the RMS error was in the range of 0.093 to 0.241.
作者机构：
[李河; 周国英; 章怀云; 刘君昂; 彭宽] Central South University of Forestry and Technology
关键词：
油茶苗圃;炭痕病菌;抗药性;研究
摘要：
The fungicide resistance of anthracnose in Camellia oleifera nurseries was investigated in Liuyang,Changning,and other regions in Hunan Province.The Colletotrichum gloeosporioides strains from the former two regions were highly resistant to carbendazim.After subcultured for 10 generations on fungicide-free medium,the resistant strains grew well on the medium containing carbendazim 450 μg/mL and suggesting that its resistance was stable.The β-tubulin genes from the resistant and susceptible strains were cloned and sequenced.The coding region was 1 344 bp nucleotides and predicted to encode a protein with 447 amino acids.Comparison of the β-tubulin amino acid sequences between resistant and susceptible strains of Colletotrichum gloeosporioides revealed that a mutation leading to an amino acid substitution at the position 198 from glutamic acid in the susceptible strain to alanine in the resistant strain.Finally,the main morphological characteristics of C.gloeosporioides were descripted,but it could not be used to determine the resistance to carbendazim.
