摘要:
采用十字交叉法测定CF17发酵液的抑菌谱,并通过离心、旋转蒸发浓缩、丙酮沉淀、固相萃取以及半制备高效液相色谱法对抑菌物质进行分离纯化和鉴定.结果表明,CF17发酵液对油茶炭疽病、油茶叶枯病、油茶软腐病、杉木炭疽病、核桃壳梭孢、降香黄檀炭疽病、檀香炭疽病等的病原菌均有抑菌效果.50%丙酮分离纯化效果优于乙醇沉淀法与乙酸乙酯沉淀法;在固相萃取中,活性物质主要集中在60%乙腈梯度洗柱样品中,最后经HPLC分离收集到一个有明显抑菌圈的样品.经质谱鉴定抑菌成分为Tetramycin A.
摘要:
Triterpene acids (TAs) are the major bioactive constituents in the medicinal fungus Ganoderma lingzhi. However, fermentative production of TAs has not been optimized for commercial use, and whether the TAs isolated from G. lingzhi submerged culture mycelia possess antitumor activity needs to be further proven. In this study, enhanced TA yield and productivity were attained with G. lingzhi using response surface methodology. The interactions of three variables were studied using a Box-Benhnken design, namely initial pH, dissolved oxygen (DO) and fermentation temperature. The optimum conditions were an initial pH of 5.9, 20.0% DO and 28.6 °C. These conditions resulted in a TA yield of 308.1 mg/L in a 5-L stirred bioreactor. Furthermore, the optimized conditions were then successfully scaled up to a production scale of 200 L, and maximum TA production and productivity of 295.3 mg/L and 49.2 mg/L/day were achieved, which represented 80.9% and 111.5% increases, respectively, compared with the non-optimized conditions. Additionally, the triterpene acid extract (TAE) from G. lingzhi mycelia was found to be cytotoxic to the SMMC-7721 and SW620 cell lines in vitro, and the TAE exhibited dose-dependent antitumor activity against the solid tumor sarcoma 180 in vivo. Chemical analysis revealed that the key active triterpene compounds, ganoderic acid T and ganoderic acid Me, predominated in the extract.
作者机构:
[刘高强; 王晓玲; Zhu, Chaoyang] College of Life Science and Technology, Central South University of Forestry &, Technology, Changsha, China;[王晓玲; 刘高强; Zhu, Chaoyang; 周国英] Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, China
通讯机构:
College of Life Science and Technology, Central South University of Forestry & Technology, Changsha, China
会议论文集名称:
International Workshop on Earth Observation and Remote Sensing Applications
关键词:
Chinese fir;disease stress;estimation;Hyperspectral;pigment content
摘要:
Using hyperspectral technology to test canopy of the fir which was damaged by the anthracnose, exploring and built the hyper-spectral estimation models of the pigment content in canopy of Chinese fir under the disease stress, the results will promote the application of the hyper-spectral remote sensing-technology in the forest pest and disease monitoring. From May to July in 2012, You Xian, Hunan Province, we investigated the anthracnose of Chinese fir, and measured the canopy pigment content and the spectral reflectance of the Chinese fir which was damaged by the anthracnose. The correlation between pigment contents of fir leaves with spectra reflectance, the first derivative of reflectance and spectral characteristic parameters were analyzed respectively. The result showed that the visible light and near-infrared region were the sensitive region which were reflected and absorbed by the pigment in disease of Chinese fir; pigment content had the highest correlation with the first order differential spectra in red edge (695-754 nm), and the correlation coefficient of single band first order differential spectrum in 741 nm was the largest. And it also showed that the accuracy of Chla+b, Chla and Chlb contents was the highest estimated by power function model which used the difference vegetation index DVI [FD587, FD741] as the variable, the relative errors were less than 15%, the RMS error was in the range of 0.093 to 0.241.
作者机构:
[李河; 周国英; 章怀云; 刘君昂; 彭宽] Central South University of Forestry and Technology
关键词:
油茶苗圃;炭痕病菌;抗药性;研究
摘要:
The fungicide resistance of anthracnose in Camellia oleifera nurseries was investigated in Liuyang,Changning,and other regions in Hunan Province.The Colletotrichum gloeosporioides strains from the former two regions were highly resistant to carbendazim.After subcultured for 10 generations on fungicide-free medium,the resistant strains grew well on the medium containing carbendazim 450 μg/mL and suggesting that its resistance was stable.The β-tubulin genes from the resistant and susceptible strains were cloned and sequenced.The coding region was 1 344 bp nucleotides and predicted to encode a protein with 447 amino acids.Comparison of the β-tubulin amino acid sequences between resistant and susceptible strains of Colletotrichum gloeosporioides revealed that a mutation leading to an amino acid substitution at the position 198 from glutamic acid in the susceptible strain to alanine in the resistant strain.Finally,the main morphological characteristics of C.gloeosporioides were descripted,but it could not be used to determine the resistance to carbendazim.
作者机构:
[Li He] Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Hunan, Peoples R China.;[Liu Junang; Zhou Guoying; Guo Liang] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.
通讯机构:
[Li He] C;Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Hunan, Peoples R China.
会议名称:
International Symposium on Manufacturing Systems Engineering (ISMSE 2011)
会议时间:
SEP 17-18, 2011
会议地点:
Hong Kong, PEOPLES R CHINA
会议主办单位:
[Li He] Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Peoples R China.^[Zhou Guoying;Guo Liang;Liu Junang] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.
会议论文集名称:
Advanced Materials Research
关键词:
L.deliciosus;GAPDH;Bioinformatics analysis
摘要:
Glyceraldehyde 3-phosphate dehydrogenase is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In the present study, some characters of the amino acid sequence of GAPDH of L.deliciosus were predicted and analyzed with the tools of bioinformatics. These results showed that the protein was composed of 20 kinds of amino acid; the theoretical pI of GAPDH was 7.08 and the theoretical molecular weight of GAPDH was 26165.9 Da; the total number of atoms was 3714. It was a stable protein. There were 7 glycosylation sites and it was a tetrameric NAD-binding enzyme involved in glycolysis and glyconeogenesis. N-terminal domain is a Rossmann NAD (P) binding fold. C-terminal domain is a mixed alpha/antiparallel beta fold.
摘要:
Plant extracts were efficient, safe bio-control stuff. In this paper we used two kinds of plant extracts, Eugenol and Baicalein, to against Colletotrichum gloeosporioides and Agaricodochium camellia by growth rate method. The results show that the inhibition effects of eugenol and baicalein were increasing as the rising concentration form 0 to 200ug/ml. The EC50 of eugenol against Colletotrichum gloeosporioides and Agaricodochium camellia were 30.37ug/ml and 51.55ug/ml respectively, the EC50 of baicalein against Colletotrichum gloeosporioides and Agaricodochium camellia were 61.26ug/ml and 49.83ug/ml respectively. The synergistic interaction values of the compounded agents with different volume ratio against Colletotrichum gloeosporioides and Agaricodochium camellia were between 0.5 and 1.5. When the volume ratio of eugenol and baicalein were 1:1 ,the prevention and treatment effects of compounded agent and carbendazim with were the same, up to 60%.
关键词:
Control method;Pathogenic mechanism;Pathogeny;Pine;Pine wilt disease
摘要:
The pine is an important tree species in China, while the pine wilt disease is a devastating disease of pine treesPine wood nematode(Bursaphelechus xylophilus Steiner & Buhere, 1934 Nickle, 1981) is a