作者机构：
[Dang-Quan ZHANG; Qing-Zhi MA] School of Resources and Environments, Central South University of Forestry and Technology, Changsha, China;[Qi-Mei LIU; Yin-She LUO; Hong WANG; Wan-Xi PENG; Yi-Qiang WU; Shui-Ping YIN] Institute of Rheological Mechanics and Material Engineering, Central South University of Forestry and Technology, Changsha, China
会议名称：
iCBBE 2008
会议时间：
2008
会议地点：
Shanghai, China
会议论文集名称：
The 2nd International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2008): Pages 3086-3603, May 16-18, 2008 Shanghai, China, [v.7]
摘要：
In order to better utilize and recover the productive wastewater from the Eucalyptus oil and hence decrease its pollution to water environment, we used GC/MS to analyze the possible top value-added components of benzene/ethanol extractives of leaves of two Eucalyptus cultivars, i.e. E. camaldulensis and E. citriodora. Relative content of each component was determined by area normalization. 22 compounds representing 92.12% of the benzene/ethanol extractive of E. camaldulensis leaves were identified, and the main constituents were 5-(acetylaminomethyl)-4-amino-2-methyl-(15.40%), hexadecanoic acid (10.25%), benzaldehyde, 4-hydroxy-(8.64%), xylitol (7.57%), cyclopentanol (7.10%), 2-propenoic acid, 3-(4-hydroxyphenyl)- (4.41%), eucalyptol (1.38%), etc. 28 compounds representing 98.41% of the benzene/ethanol extractive of E. citriodora leaves were also identified, and the main constituents were ethanol, 2-butoxy- (21.70%), benzene, methyl (1-methylethyl)- (15.61%), 17-octadecenal (15.25%), 1-hexacosanal (9.45%), 1-eicosanol (6.92%), cyclotetracosane (6.67%), alpha.-phellandrene (3.75%), etc. The results of function analyses showed that the benzene/ethanol extractives of leaves of both E. camaldulensis and E. citriodora are abundant in materials of noble natural medicines, and also contains constituents which can be developed into top value-added materials of cosmetic, biofuel and industrial solvent.
摘要：
<jats:p>The chemical components of helium volatiles from the fresh branches of <jats:italic>Cinnamomum camphora</jats:italic> were studied by TD-GC/MS. The analytical result by 60°С-based TD-GC/MS showed that 55 peaks were obtained from the helium volatiles from the fresh branches of <jats:italic>Cinnamomum camphora</jats:italic> and 53 chemical compounds were identified. The results showed that the main components were as: Bicyclo[2.2.1]heptan-2-one, 1,7,7-trimethyl-, (1R)- (15.4328%), 1,3-Benzodioxole, 5-(2-propenyl)- (14.881%), Tricyclo[2.2.1.0(2,6)]heptane, 1,7-dimethyl-7-(4-methyl-3-pentenyl)-, (-)- (12.694%), p-menth-1-en-8-ol (9.832%), Bicyclo[2.2.1]heptane, 2-methyl-3-methylene-2-(4-methyl-3- pentenyl)-, (1S-exo)- (6.143%), 1,6,10-Dodecatrien-3-ol, 3,7,11-trimethyl- (5.365%), Bicyclo[3.1.1] hept-2-ene, 2,6-dimethyl-6-(4-methyl-3-pentenyl)- (4.527%), Naphthalene, 1,2,3,5,6,8a- hexahydro-4,7-dimethyl-1-(1-methylethyl)-, (1S-cis)- (4.129%), 3-Cyclohexen-1-ol, 4-methyl-1- (1-methylethyl)- (2.965%), Borneol (2.627%), Bicyclo[2.2.1]heptan-2-ol, 1,7,7-trimethyl-, acetate, (1S-endo)- (2.586%), Copaene (2.534%), 1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene-, (Z)- (1.612%), (-)-Isosativene (1.121%), etc. The analytical result suggested that the helium volatiles from the fresh branches of <jats:italic>Cinnamomum camphora</jats:italic> could be used as industrial materials of biomedicines and spicery.</jats:p>
摘要：
<jats:p>The extractives of <jats:italic>C. camphora</jats:italic> old bark were used to cure some special diseases; however, the biomedical constituents of extractives of <jats:italic>C. camphora</jats:italic> old bark are still not completely explained. Therefore, 350 °C-based pyrolysis- GC/MS technology was used to identify the top value-added biomedical constituents of old bark-based benzene/ethanol extractives from <jats:italic>C. camphora</jats:italic> trunk. 74 chemical constituents representing 99.17% were identified from 86 peaks. The main components are as: Dodecanoic acid, 1-(hydroxymethyl)-1,2-ethanediyl ester (15.717%), Octacosanoic acid, methyl ester (10.357%), Abietic acid (8.483%), Heptacosane (5.678%), Ethyl alcohol (5.282%), Hexadecanoic acid, 2-hydroxy-, methyl ester (3.365%), Hexacosanoic acid, methyl ester (2.936%), 1,2-Benzenedicarboxylic acid, butyl 2-methylpropyl ester (2.928%), 2-Methoxy-4-vinylphenol (2.692%), Hexadecanoic acid, ethyl ester (2.312%), Hexacosane (2.076%), Tetracosanoic acid, methyl ester (2.013%), etc. The analytical result showed that the 350 °C pyrolyzate of benzene/ethanol extractives from <jats:italic>C. camphora</jats:italic> old bark is abundant in biomedical constituents, and also contains other bioactive components, which can be used as top value-added materials of high-grade cosmetic, food, spice and chemical solvents.</jats:p>
摘要：
The extractives of bamboo root was often used to cure some intractable diseases in the countryside of China, but the components of bamboo root extract are still unresolved. Therefore, 300 °C-based pyrolysis- GC/MS technology was used to identify and analyze the top value-added biomedical and bioactive components of benzene/ethanol extractives of Moso bamboo root. 45 compounds representing 97.316 % of the total areas were identified (Table 1) from 55 peaks. The main chemical components of benzene/methanol extractives of Moso bamboo root by 300°C-based Pyrolysis- GC/MS are as: 1,3-BENZODIOXOLE, 5-(1-PROPENYL)- (15.263%), NAPHTHALENE (12.316%), 1,2-BENZENEDICARBOXYLICACID,BIS(2-METHYLPROPYL) ESTER (9.385%), BENZENE, 1,2-DIMETHOXY-4-(2-PROPENYL)- (7.624%), 2-PROPENOIC ACID, 3-(2-HYDROXYPHENYL)- (E)- (6.012%), 4-HYDROXY-2-METHYLACETOPHENONE (5.226%), 1,2-BENZENEDICARBOXYLIC ACID, BUTYL2-METHYLPROPYL ESTER (4.328%), etc. The result showed that the 300 °C pyrolyzate of benzene/ethanol extractives of Moso bamboo root is rich in biomedical components, and also contains some bioactive components which can be used as top value-added materials of high-grade spice, cosmetic and food.
摘要：
<jats:p>In order to better utilize and recover the productive waste leaves from the Cinnamomum camphora, pyrolysis- GC/MS technology was used to analyze the possible top value-added components of benzene/ethanol extractives of <jats:italic>Cinnamomum camphora</jats:italic> leaves. Relative content of each component was determined by area normalization. 95 compounds from 102 peaks representing 99.216% of the benzene/ethanol extractives of <jats:italic>Cinnamomum camphora</jats:italic> leaves were identified, and the main constituents were Hexadecanoic acid, 4-nitrophenyl ester (17.336%), Vitamin E (4.804%), Oxirane, hexadecyl- (4.639%), 2,6-Bis(3,4-methylenedioxyphenyl) -3,7-dioxabicyclo(3.3.0)octane (4.333%), 1-Hexacosene (4.148%), Hexadecanoic acid, 2-methoxy-, methyl ester (3.783%), 1,19-Eicosadiene (3.607%), 3-Cyclohexene-1- methanol, .alpha.,.alpha.4-trimethyl- (3.542%), .gamma.-Sitosterol (3.495%), Nonacosane (3.217%), .gamma.-Tocopherol (3.119%), Eucalyptol (2.23%), vanillin (2.742%), Heptacosane (2.182%), Pentatriacontane (2.039%), 1-Nonadecene (2.011%), etc. The results of function analyses showed that the 350 °C pyrolyzate from benzene/ethanol extractives of waste leaves of <jats:italic>Cinnamomum camphora </jats:italic>are abundant in materials of noble natural medicines and cosmetic.</jats:p>
摘要：
<jats:p>
<jats:italic>Pinus massoniana</jats:italic> Lamb has been used to heal some specific diseases in the Chinese Folk for a long time. In order to explore the wide utilization in biomedicine and spicery, the chemical components of helium extractives from the fresh wood of <jats:italic>Pinus massoniana</jats:italic> Lamb was studied by TD-GC/MS. The results showed that the main components were 1,4-Methanoazulene, decahydro-4,8, 8-trimethyl-9-methylene- (29.98%), 1,4-Methanoazulen-9-ol, decahydro-1,5,5,8a-tetramethyl- (10.32%), 1R-.alpha.-Pinene (8.33%), 1,2,4-Methenoazulene, decahydro-1, 5,5,8a-tetramethyl- (4.72%), Borneol (2.99%), Thujopsene (2.80%), 1-Phenanthrenecarboxaldehyde, 7-ethenyl-1,2,3,4,4a,4b, 5,6,7,9,10,10a- dodecahydro-1,4a,7-trimethyl- (2.53%), Bicyclo[2.2.1]heptan-2-one, 1,7,7- trimethyl-, (1R)- (2.52%), Caryophyllene oxide (2.40%), Tricyclo[5.4.0.0(2,8)]undec-9-ene, 2,6,6,9-tetramethyl- (2.32%), 1,3-Benzodioxole, 5-(2-propenyl)- (2.274%), Acetic acid (2.07%), Eucalyptol (1.70%), Eucalyptol (1.70%), Benzaldehyde, 4-hydroxy- (1.61%), etc. The analytical result suggested that the helium extractives from the fresh wood of <jats:italic>Pinus massoniana</jats:italic> Lamb could be used as industrial materials of biomedicines and spicery.</jats:p>
作者机构：
[Qi-Mei Liu; Wan-Xi Peng] Institute of Rheological Mechanics and Material Engineering, Central South University of Forestry and Technology, Changsha, China;[Dang-Quan Zhang; Lin-Lin Guo] Key Laboratory of Non-wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha, China
会议名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)
会议时间：
2010-06-18
会议地点：
成都
会议论文集名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)论文集
摘要：
The oil-tea cake as the byproduct of tea-oil from oil-tea seed is still not top-valued used. In order to improve the processing status of oil-tea cake from low-grade to high-grade, we used 300°C pyrolysis- GC/MS technology to analyze the top value-added biomedical and bioactive constituents of two extracts (benzene/ethanol and acetone) of oil-tea cake. Relative content of each component was determined by area normalization. 13 compounds representing 99.18% of the benzene/ethanol extract of oil-tea cake were identified, and 28 compounds representing 98.62% of the acetone extract of oil-tea cake were also identified, The results of function analyses showed that the benzene/ethanol extract and acetone extract of oil-tea cake contain abundant components of rare natural medicinal materials, and also contain many components which can be developed into two value-added materials of high-grade spice, cosmetic, food, and industrial chemical and solvent, This result will benefit the status change of processing and utilization of oil-tea cake in China from low-grade to high-grade, and hence is helpful to the sustainable development of oil-tea industry of China.
摘要：
The oil-tea cake as the byproduct of tea-oil from oil-tea seed is still not top-valued used. In order to improve the processing status of oil-tea cake from low-grade to high-grade, we used 300 degrees C pyrolysis- GC/MS technology to analyze the top value-added biomedical and bioactive constituents of two extracts (benzene/ethanol and acetone) of oil-tea cake. Relative content of each component was determined by area normalization. 13 compounds representing 99.18% of the benzene/ethanol extract of oil-tea cake were identified, and 28 compounds representing 98.62% of the acetone extract of oil-tea cake were also identified, The results of function analyses showed that the benzene/ethanol extract and acetone extract of oil-tea cake contain abundant components of rare natural medicinal materials, and also contain many components which can be developed into two value-added materials of high-grade spice, cosmetic, food, and industrial chemical and solvent, This result will benefit the status change of processing and utilization of oil-tea cake in China from low-grade to high-grade, and hence is helpful to the sustainable development of oil-tea industry of China.
摘要：
China has the rich bamboo resource, moreover, the species and yield of bamboo both are the first in the world. The extract of bamboo root was often used to cure some intractable diseases in the countryside of China, but the components of bamboo root extract is still unresolved. In order to expedite medicinal application of bamboo root, and to provide further scientific foundation for its top value-added development, pyrolysis- GC/MS technology was used to identify and analyze the top value-added biomedical and bioactive components of benzene/ethanol extract of Moso bamboo root. The extract was pyrolyzed at 750 °C in helium atmosphere, and then the pyrolyzate was analyzed by online-linked GC/MS. Relative content of each component was determined by area normalization. 87 compounds representing 98.31% were identified from 105 peaks. The result showed that the pyrolyzate of benzene/ethanol extract of Moso bamboo root is rich in biomedical components, and also contains some bioactive components which can be used as top value-added materials of high-grade spice, cosmetic, food, and chemical.
期刊：
2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11,2009年:712-715 ISSN：2151-7614
通讯作者：
Zhang, Dang-Quan
作者机构：
[Zhang, Dang-Quan; Huang, Qing-Yun; Deng, Shun-Yang; Gu, Zheng-Jun; Fan, Shao-Gang] Cent South Univ Forestry & Technol, State Forestry Adm, Key Lab Nonwood Forest Prod, Biotechnol Core Facil, Changsha 410004, Hunan, Peoples R China.;[Ma, Qing-Zhi; Liu, Qi-Mei] Cent South Univ Forestry & Technol, Inst Rheolog Mech & Mat Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zhang, Dang-Quan] C;Cent South Univ Forestry & Technol, State Forestry Adm, Key Lab Nonwood Forest Prod, Biotechnol Core Facil, Changsha 410004, Hunan, Peoples R China.
会议名称：
iCBBE 2009
会议时间：
2009-01-01
会议地点：
Beijing, China
会议论文集名称：
The 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2009). [v.2]
关键词：
carrot antifreeze protein;cryopreservation;ice surface;surface complementarily;engineered protein
摘要：
The
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expressed carrot antifreeze protein (DcAFP) was purified by preparative HPLC, refolded by dialysis, and checked by analytical HPLC and SDS-PAGE. The gradient-concentrations of DcAFP in 3% glycerol were used to cryopreserve rice single cell by vitrification at -25degC, -75degC and -180degC. The result showed that DcAFP, at optimal concentration of 0.1 mg/ml, can visibly inhibit the liquid recrystallization of thawed rice cells. DcAFP treated rice cells had higher viability even than positive control and facilitated the efficient daughter cell division. The average diameter of inhibited ice crystals was about 1.0 mum that was far less than that of rice cell, and was the reason for the decrease of the rice cell damage. The result of molecular dynamics simulations suggested that DcAFP can adsorb into 1010 plane of ice crystal to form an imperfect surface complementarity, hence to inhibit the growth and recrystallization of ice granules. We modeled the interactions among rice cell, DcAFP and ice, and suggested that DcAFP can stack around the cell membrane by interaction. Therefore, the adsorbing of DcAFP to the ice surfaces can protect the rice cells from the damage of the ice crystals.
