期刊论文、会议论文

Zhang, Dang-Quan

英文

2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11

2151-7614

2009

712-715

iCBBE 2009

The 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2009). [v.2]

2009-01-01

Beijing, China

IEEE

978-1-4244-2901-1

10.1109/ICBBE.2009.5162384

Natural Science Foundation of China [30700643]; Hunan Natural Science Foundation of China [05JJ40125]

本校为第一且通讯机构

The E. coli expressed carrot antifreeze protein (DcAFP) was purified by preparative HPLC, refolded by dialysis, and checked by analytical HPLC and SDS-PAGE. The gradient-concentrations of DcAFP in 3% glycerol were used to cryopreserve rice single cell by vitrification at -25degC, -75degC and -180degC. The result showed that DcAFP, at optimal concentration of 0.1 mg/ml, can visibly inhibit the liquid recrystallization of thawed rice cells. DcAFP treated rice cells had higher viability even than positive control and facilitated the efficient daughter cell division. The average diameter of in...