摘要：
Several Eucalyptus cultivars has been introduced and cultivated for millions of hectares afforestation in subtropical China, whose woods were mainly used in papermaking industry and whose water essential oils were used mainly in aroma and medical industry. However, the potential biomedical components of organic essential oils were still uncovered. In order to reveal constituent characteristic and biomedical potentials of organic essential oils from leaves of four Eucalyptus cultivars (E. camaldulensis, E. tereticornis, E. citriodora and E. dunnii) in China, their components were efficiently extracted by improved solvents extracting condition, which were identified by optimal gas chromatography / mass spectrometry (GC/MS). The functional analytical results suggested that these four Eucalyptus cultivars possess large numbers of common components, especially including Eucalyptol, 1-Eicosanol, (+)-Longifolene, (+)-Aromadendrene, Phytol and (-)-Alloaromadendrene, which are good materials for aroma and biomedical industry. The contents of common components of four Eucalyptus cultivars are as high as 68.97%, 81.81%, 60.64% and 64.29%, respectively. Moreover, each Eucalyptus organic essential oil also has specific components with potentials in aroma industry or biomedical industry.
作者机构：
[李河; 周国英; 章怀云; 刘君昂; 彭宽] Central South University of Forestry and Technology
关键词：
油茶苗圃;炭痕病菌;抗药性;研究
摘要：
The fungicide resistance of anthracnose in Camellia oleifera nurseries was investigated in Liuyang,Changning,and other regions in Hunan Province.The Colletotrichum gloeosporioides strains from the former two regions were highly resistant to carbendazim.After subcultured for 10 generations on fungicide-free medium,the resistant strains grew well on the medium containing carbendazim 450 μg/mL and suggesting that its resistance was stable.The β-tubulin genes from the resistant and susceptible strains were cloned and sequenced.The coding region was 1 344 bp nucleotides and predicted to encode a protein with 447 amino acids.Comparison of the β-tubulin amino acid sequences between resistant and susceptible strains of Colletotrichum gloeosporioides revealed that a mutation leading to an amino acid substitution at the position 198 from glutamic acid in the susceptible strain to alanine in the resistant strain.Finally,the main morphological characteristics of C.gloeosporioides were descripted,but it could not be used to determine the resistance to carbendazim.
摘要：
Tung oil is a special biomass resource in China. Tung Oil has the characteristic to be transformed into serial derivatives for its active Conjugated double bond. Especially, tung oil anhydride-ester polyol (TOAEP) can be used as substitute of polyester polyol and/or polyether polyol in the synthesis and modification of polyurethane materials. Through esterification and transesterification, methyl-α-eleostearate-maleic anhydride adduct (MEMAA), a derivative of tung oil, was reacted with ethylene glycol and tung oil anhydride-ester polyol (TOAEP) was obtained. The optimal preparation condition are as follows: p-toluene sufonic acid is used as the catalyst, mole ratio of ethylene glycol to MEMAA is 4:1, reaction temperature is 120°C ~130°C, reaction duration is 8 h. Under the optimal preparation conditions, the yield of TOAEP is up to 88.8% and the hydroxyl value is up to 318.0 mg KOH/g. The structure of TOAEP was confirmed by Infrared spectroscopy (IR).
关键词：
Control method;Pathogenic mechanism;Pathogeny;Pine;Pine wilt disease
摘要：
<jats:p>The pine is an important tree species in China, while the pine wilt disease is a devastating disease of pine trees. Pine wood nematode (<jats:italic>Bursaphelechus xylophilus </jats:italic>Steiner & Buhere, 1934 Nickle, 1981) is a world recognized major alien species in the world. It’s from the United States, but pine wood nematode does not seriously endanger the pine in China; the most endangered place is Japan, after several decades of research and control, it has been basically able to control the occurrence of pine wilt disease. The spread trend of pine wilt disease in China grows more and more obvious, which has become an important intrusion pathogenic organism. It not only causes a devastating threats the million hectares pine forest in southern China, but also affects China’s economy and social sustainable development, what’s more, it will damage some of famous scenic spots and cultural heritage sites in China. Meanwhile, the pine wood nematode is a technical barrier, which seriously impacts on China’s import and export trade. The pathogen, pathogenesis, modes of transmission and means of distribution patterns, rapid detection and control methods of pine wilt disease are reviewed in this paper, and we hope that it can provide references for effective control of pine wilt disease in China.</jats:p>
作者机构：
[Zhou Guoying; Zhang Huaiyun; Li He] Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha 410004, Hunan, Peoples R China.;[Liu Yadi; Liu Wenjin] Cent South Univ Forestry & Technol, Coll Furniture & Art Design, Changsha, Peoples R China.
通讯机构：
[Li He] C;Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha 410004, Hunan, Peoples R China.
会议名称：
International Conference on Micro Nano Devices, Structure and Computing Systems
会议时间：
NOV 06-07, 2010
会议地点：
Int Sci & Engn Ctr, Singapore, SINGAPORE
会议主办单位：
Int Sci & Engn Ctr
会议论文集名称：
Advanced Materials Research
关键词：
Bamboo fiber;Bleach;Crude fiber;Pretreatment
摘要：
Bamboo fiber is a new natural green environmental protection fiber material with good characteristics of good permeability, unique elasticity, transient absorption, antibacterial, odor resistance and stronger vertical and horizontal intensity. Bamboo pretreatment can improve the biological degumming efficiency of bamboo lignin, reduce microbial degumming time, and reduce production costs. The results show that the removal efficiency of pectin, hemicelluloses and lignin are better after the pretreatment of bamboo mercerization. Through orthogonal experiments, it can be got the optimum combination of bamboo fiber alkali cooking process is A2-B2-C2-D1, namely: concentration of NaOH is 6.0g / L, its time is 60min, temperature 100 °C, and bath ratio 1:20. In these four factors, the greatest impact on the removal of lignin is temperature, followed by is degumming time, then the concentration of alkali, and the bath ratio is the smallest. The best conditions of bamboo fiber bleaching is: hydrogen peroxide 25 ~ 35g / L, temperature 30 ~ 40 °C, treatment 45 ~ 60min. The process of the pretreatment of bamboo is: clip → Roll → high-pressure steaming → roll→ immersion separation → alkali leaching treatment→ scouring → rolling combing →washing→ drying →crude fiber → bleaching.
摘要：
Fluorescent quantum dots (QDs) have shown great promise for use as biolabels in cell and animal biology and more recently in plant sciences. An important use of QDs is for monitoring the dynamics, intracellular trafficking, and fate of carrier-DNA nanocomplexes in cell transfection and potentially in plant transformation. In this study, a low cost aqueous procedure has been developed to efficiently prepare biocompatible QDs for monitoring nanoparticle-mediated gene transfer in conjunction with molecular breeding of Jatropha curcas. Water-soluble CdSe nanoparticles were synthesized by self-assembly using L-Cysteine as stabilizer and optimal synthesis scheme established by fluorescence spectroscopy. The QDs were used to label chitosan-DNA nanoparticles via electrostatic interaction and the resultant QD-labeled chitosan-DNA complexes were shown to have superior fluorescence properties with red shift of emission and absorption spectra relative to the CdSe QDs alone. This system is being explored as a superior alternative to Agrobacterium-mediated genetic transformation of Jatropha curcas cells. PCR amplification of the full length of the carried reporter gene (GFP) suggests that the DNA was not digested in Jatropha curcas cells transfected with CdSe/CS-DNA complexes. Furthermore, GFP gene expression in the transfected callus cells, as evidenced by fluorescence detection, suggests that the target DNA was integrated into the plant genome.
摘要：
The extractives of bamboo root was often used to cure some intractable diseases in the countryside of China, but the components of bamboo root extract are still unresolved. Therefore, 300 °C-based pyrolysis- GC/MS technology was used to identify and analyze the top value-added biomedical and bioactive components of benzene/ethanol extractives of Moso bamboo root. 45 compounds representing 97.316 % of the total areas were identified (Table 1) from 55 peaks. The main chemical components of benzene/methanol extractives of Moso bamboo root by 300°C-based Pyrolysis- GC/MS are as: 1,3-BENZODIOXOLE, 5-(1-PROPENYL)- (15.263%), NAPHTHALENE (12.316%), 1,2-BENZENEDICARBOXYLICACID,BIS(2-METHYLPROPYL) ESTER (9.385%), BENZENE, 1,2-DIMETHOXY-4-(2-PROPENYL)- (7.624%), 2-PROPENOIC ACID, 3-(2-HYDROXYPHENYL)- (E)- (6.012%), 4-HYDROXY-2-METHYLACETOPHENONE (5.226%), 1,2-BENZENEDICARBOXYLIC ACID, BUTYL2-METHYLPROPYL ESTER (4.328%), etc. The result showed that the 300 °C pyrolyzate of benzene/ethanol extractives of Moso bamboo root is rich in biomedical components, and also contains some bioactive components which can be used as top value-added materials of high-grade spice, cosmetic and food.
摘要：
<jats:p>Py-GC/MS technology was used to analyze the high-grade resource recovering approaches of C. camphora root wood at 350°C. The result showed that the chemical components of acetone extractives from <jats:italic>C. camphora</jats:italic> root wood are as: Benzene, 1,2-dimethoxy-4-(2-propenyl)- (10.255%), Bicyclo[2.2.1]heptan-2-one, 1,7,7-trimethyl-, (1R)- (7.751%), p-menth-1-en-8-ol (5.765%), Phenol, 2,6-dimethoxy-4-(2-propenyl)- (3.833%), 1,6-Cyclodecadiene, 1-methyl-5-methylene-8- (1-methylethyl)-, [s-(E,E)]- (3.669%), D-Allose (3.265%), Acetic acid (2.784%), 1(2H)-Naphthalenone, octahydro-8a-hydroxy- (2.719%), 1,3-Benzodioxole, 4-methoxy-6- (2-propenyl)- (2.674%), .beta.(3,4-Methylenedioxyphenyl)propionic acid (2.216%), Phenol, 4-methyl- (2.200%), Phenol, 2,6-dimethoxy- (2.138%), 3,4-Methylenedioxyphenyl acetone (2.125%), 5-Hepten-2-one, 6-methyl- (2.075%), 2-Oxabicyclo[2.2.2]octan-6-ol, 1,3,3-trimethyl- (1.808%), Phenol, 2-methoxy-3-(2-propenyl)- (1.688%), etc. The analytical result by 350°C-based Py-GC/MS showed that the 350°C pyrolyzate of acetone extractives from <jats:italic>C. camphora</jats:italic> root wood can be used as top value-added materials of biomedicine, but also used as the bioactive materials of bioenergy, cosmetic, food, and other industrial solvents.</jats:p>
