期刊论文

中文

中国农学通报

1000-6850

2012

28

31

146-152

10.3969/j.issn.1000-6850.2012.31.027

林业公益性行业科研专项“麻疯树基因工程与抗寒育种研究”（200904038）；

本校为第一机构

抗冻蛋白（antifreeze protein,AFP）是一类能控制冰晶生长和抑制冰晶之间发生重结晶的蛋白质,能在低温结冰条件下保护生物体不受伤害。抗冻蛋白具有2种明显不同的抗冻活性——热滞（thermalhysteresis,TH）活性和重结晶抑制（recrystallizationinhibition,RI）活性。鱼类和昆虫抗冻蛋白的特征是高TH低RI活性,而植物抗冻蛋白的特征是低TH高RI活性。为了获得高TH、高RI活性的抗冻蛋白（antifreeze protein,AFP）,克隆得到胡萝卜抗冻蛋白基因（DcAFP）和黄粉虫抗冻蛋白基因（TmAFP）cDNA全序列。通过重叠延伸PCR,将2个基因前后串联构建融合基因Dc-TmAFP和Tm-DcAFP,并构建了植物表达载体,通过农杆菌...

Antifreeze proteins（AFPs） are a family of proteins capable of protecting organisms from damage in freezing or sub-freezing conditions by controlling the growth of ice and inhibiting the recrystallization between ice granules,which are termed thermal hysteresis（TH） activity and recrystallization inhibition（RI） activity respectively.In comparison with the characteristic of high TH and low RI of fish AFPs and insect AFPs,antifreeze proteins of plants was low TH and high RI activity.In order to obtain antifreeze proteins with high TH and high...