期刊论文

中文

林业科学

1001-7488

2013

49

2

127-133

10.11707/j.1001-7488.20130219

201104003:林业公益性行业科研专项 2011AA100202:国家高技术研究发展计划（863计划） 2010NK2008,2011NK2019:湖南省科技厅重点项目 2011KJCX019:广东省林业创新专项

本校为第一机构

桉树是桃金娘科(Myrtaceae)桉属(Eucalyptus) 、杯果木属(Angophora)和伞房属(Corymbia)3个属的树种总称,约有945种。桉树具有生长迅速、轮伐期短、耐干旱、耐贫瘠、适应性广等优良特性,且用途广、经济效益高,因此,桉树现已被世界近百个国家与地区引种,遍布于地中海地区、东南亚、美洲和非洲,目前已经成为世界第二大类造林树种(陈少雄等,2008; 欧阳乐军等,2008; 祈述雄,2006) 。我国自1890年引种桉树以来,已有120多年的历史,种植面积不断增长,至2010年底,我国桉树人工林面积超过368万hm~2(谢耀坚,2011) 。

Based on the relevant properties of EST-SSR gene function, and the high polymorphism and potential regulatory function of Genomic-SSR, the polymorphism effect and transferability of EST-SSR and genomic-SSR of eucalypt trees was comparatively studied in this article. A genomic DNA pooling method was developed to rapidly screen eucalypt SSR primers. A total of 340 pairs of eucalypt SSR primers were screened from 395 pairs designed by optimizing SSR-PCR system, and the screening ratio was 86.06%. Genomic-SSR and EST-SSR screened 204 pairs （ratio ...