Some natural ingredients, extracted from animals, plants and microorganisms, have diversity in structure and bio-activity, and play an important role in developing new medicine and medicine lead compounds. Newly-emerging separation technologies will change the unfavorable situation where traditional ones are of low efficiency and high cost. In order to provide some references for development and application of active constituents from natural products, the research progress of extraction and separation technologies of active constituents from natural products at home and abroad were reviewed, ...