关键词:
全自动间断化学分析仪, 原子吸收光谱法, 油茶, 授粉受精, 矿质元素, Auto discrete analyzers, Atomic absorption spectrophotometer, Camellia oleifera, Pollination and fertilization, Mineral elements
摘要:
为探明油茶授粉受精期的营养元素需求规律, 应用全自动间断化学分析仪与原子吸收光谱法测定了授粉受精期的油茶子房主要矿质元素的含量, 并且对各矿质元素含量在自交和异交情况下的变化进行了研究分析。 结果表明: 在油茶授粉受精过程中, 九种矿质营养元素含量呈“S”或“W”型曲线变化。 N, K, Zn, Cu, Ca, Mn元素含量变化在自交和异交情况下都呈“S”型曲线, N含量在自花授粉20 d达到最高, 为3.445 8 mg·g-1; K含量在异花授粉20 d达到最高, 为6.275 5 mg·g-1; Zn含量在自花授粉10 d达到最高, 为0.070 5 mg·g-1; Cu含量在异花授粉5 d到最高, 为0.061 0 mg·g-1; Ca含量在异花授粉15d达到最高, 为3.714 5 mg·g-1; Mn含量在自花授粉30 d达到最高, 为2.161 5 mg·g-1。 Fe, P, Mg元素含量变化自交时呈“S”, 异交时呈“W”型曲线, Fe含量在自花授粉10 d达到最高, 为0.453 0 mg·g-1; P含量在自花授粉20 d达到最高, 为6.731 8 mg·g-1; Mg含量在自花授粉25 d达到最高, 为2.724 0 mg·g-1。 研究结果可为油茶花期喷施叶面肥, 提高油茶结实率和产量提供了一定的资料参考。 In order to elucidate the nutrition of Camellia oleifera at pollination and fertilization stages, the contents of mineral elements were determined by auto discrete analyzers and atomic absorption spectrophotometer, and the change in the contents of mineral elements was studied and analysed under the condition of self- and cross-pollination. The results are showed that nine kinds of mineral elements contents were of “S” or “W” type curve changes at the pollination and fertilization stages of Camellia oleifera. N, K, Zn, Cu, Ca, Mn element content changes showed “S” curve under the self- and out-crossing, the content of N reaching the highest was 3.445 8 mg·g-1 in self-pollination of 20 d; K content reaching the highest at the cross-pollination 20 d was 6.275 5 mg·g-1; Zn content in self-pollination of 10 d reaching the highest was 0.070 5 mg·g-1; Cu content in the cross-pollination of 5 d up to the highest was 0.061 0 mg·g-1; Ca content in the cross-pollination of 15 d up to the highest was 3.714 5 mg·g-1; the content of Mn reaching the highest in self-pollination 30 d was 2.161 5 mg·g-1. Fe, P, Mg element content changes was of “S” type curve in selfing and was of “W” type curve in outcrossing, Fe content in the self-pollination 10 d up to the highest was 0.453 0 mg·g-1; P content in self-pollination of 20 d reaching the highest was 6.731 8 mg·g-1; the content of Mg up to the highest in self-pollination 25 d was 2.724 0 mg·g-1. The results can be used as a reference for spraying foliar fertilizer, and improving seed setting rate and yield in Camellia oleifera.
作者:
Cao, Heping*;Zhang, Lin;Tan, Xiaofeng;Long, Hongxu;Shockey, Jay M.
期刊:
PLOS ONE,2014年9(2):e88409 ISSN:1932-6203
通讯作者:
Cao, Heping
作者机构:
[Shockey, Jay M.; Cao, Heping] ARS, USDA, So Reg Res Ctr, Commod Utilizat Res Unit, New Orleans, LA 70130 USA.;[Long, Hongxu; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha, Hunan, Peoples R China.
通讯机构:
[Cao, Heping] A;ARS, USDA, So Reg Res Ctr, Commod Utilizat Res Unit, New Orleans, LA 70130 USA.
摘要:
Triacylglycerols (TAG) are the major molecules of energy storage in eukaryotes. TAG are packed in subcellular structures called oil bodies or lipid droplets. Oleosins (OLE) are the major proteins in plant oil bodies. Multiple isoforms of OLE are present in plants such as tung tree (Vernicia fordii), whose seeds are rich in novel TAG with a wide range of industrial applications. The objectives of this study were to identify OLE genes, classify OLE proteins and analyze OLE gene expression in tung trees. We identified five tung tree OLE genes coding for small hydrophobic proteins. Genome-wide phylogenetic analysis and multiple sequence alignment demonstrated that the five tung OLE genes represented the five OLE subfamilies and all contained the "proline knot" motif (PX5SPX3P) shared among 65 OLE from 19 tree species, including the sequenced genomes of Prunus persica (peach), Populus trichocarpa (poplar), Ricinus communis (castor bean), Theobroma cacao (cacao) and Vitis vinifera (grapevine). Tung OLE1, OLE2 and OLE3 belong to the S type and OLE4 and OLE5 belong to the SM type of Arabidopsis OLE. TaqMan and SYBR Green qPCR methods were used to study the differential expression of OLE genes in tung tree tissues. Expression results demonstrated that 1) All five OLE genes were expressed in developing tung seeds, leaves and flowers; 2) OLE mRNA levels were much higher in seeds than leaves or flowers; 3) OLE1, OLE2 and OLE3 genes were expressed in tung seeds at much higher levels than OLE4 and OLE5 genes; 4) OLE mRNA levels rapidly increased during seed development; and 5) OLE gene expression was well-coordinated with tung oil accumulation in the seeds. These results suggest that tung OLE genes 1-3 probably play major roles in tung oil accumulation and/or oil body development. Therefore, they might be preferred targets for tung oil engineering in transgenic plants.
期刊:
Advance Journal of Food Science and Technology,2013年5(12):1590-1595 ISSN:2042-4868
通讯作者:
Yuan, D.-Y.
作者机构:
[Lin Zhang; Xiao-Feng Tan; Feng Zou; De-Yi Yuan] The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China;[Lin Zhang] Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT 06269, United States;[Jing-Hua Duan] Non-timber Forest Research and Development Center of Chinese Academy of Forestry, Zhengzhou, 450003, China
通讯机构:
[Yuan, D.-Y.] T;The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, China
通讯机构:
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, China
作者机构:
Key Lab. of Non-wood Forest Product of Forestry Ministry, College of Forestry, Central South University of Forestry and Technology, Changsha city, 410004, China;Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, Liaoning, China
通讯机构:
Key Lab. of Non-wood Forest Product of Forestry Ministry, College of Forestry, Central South University of Forestry and Technology, China
关键词:
Environmental impacts;Gametophytic self-incompatibility;Pear;Rapid amplification of cDNA ends;S 29 -RNase