期刊论文

Establishment of regeneration system for mature embryo in Verniciafordii

中文

经济林研究

1003-8981

2012

30

4

119-122

10.14067/j.cnki.1003-8981.2012.04.036

国家林业公益性行业科研专项（201204403,200904023） 湖南省自然科学基金项目（10JJ4022）

本校为第一机构

为了建立快速有效的油桐再生体系,以“葡萄桐”种胚为试材,研究了贮藏方式、消毒时间、培养基及培养条件对油桐种胚成苗的影响.结果表明:种子沙藏与在自然条件下贮藏对油桐种胚成苗的影响无明显差异；种胚的最佳消毒处理为0.1％的升汞浸泡3 min;将种胚接种在1/2MS+IAA 0.5 mg/L的培养基中成苗率最高,为98％;在培养基中加入6-BA易使种胚产生大量的愈伤组织,不利于油桐胚再生体系的建立；培养基的最适pH值为5.5；与遮光处理相比,光照能缩短种胚再生体系建立的周期.

In order to establish quickly and efficiently regeneration system in Verniciafordii, using embryos of ＂Putaotong＂ as materials, effects of storage method, sterilizing time, culture mediums and culture conditions on regeneration system in V. fordii were studied. The results show that no significant difference is presented between sand storage and natural conditions on establishment of regeneration system for embryo in Efordii. The optimal disinfection time of embryos is 3 minutes by 0.1% mercuric chloride. The appropriate medium of embryo is 1...