期刊论文

中文

中南林业科技大学学报

1673-923X

2012

32

2

89-94

10.14067/j.cnki.1673-923x.2012.02.017

国家林业公益性行业科研专项（200904024） 中南林业科技大学大学生创新性实践教学项目

本校为第一机构

以云实当年生嫩叶为试材,设计正交试验优化脱分化和再分化培养基配方,获得体细胞胚并诱导不定芽;筛选最佳生根培养基,获得完整无菌植株并移栽成活。试验结果表明,最佳诱导愈伤组织培养基为:1/2MS+TDZ 0.5 mg.L~(-1)+IBA0.3 mg.L~(-1),且该培养基也适合云实叶片愈伤组织再分化出芽;添加0.1%活性碳能抑制褐化,同时促进体细胞胚发生和不定芽产生;1/2MS+NAA 0.1 mg.L~(-1)最适宜云实无菌苗生根,其根系数量质量均优于其他生根培养基组合;移栽采用蛭石∶珍珠岩∶草炭灰(1∶1∶1)基质,成活率达85%。

By taking one-year young leaves of Caesalpinia decapetala as testing materials, an orthogonal test was designed to optimize dedifferentiation and re-differentiation medium formula, obtain somatic embryos and induce adventitious buds. The best rooting medium was screened out and the sterile plants were transplanted. The results show that the best induction medium was 1/2 MS ＋ TDZ 0.5 mg·L -1 ＋IBA0.3 mg·L -1 , the medium was also suitable for differentiating Caesalpinia callus. Adding 0.1% activated carbon not only inhibited browning but also...