油茶柱头和花柱的显微与超微结构特征
作者：
高超;杨瑞;郭其强;袁德义
期刊：
林业科学研究 ,2019年32(1):1-7 ISSN：1001-1498
通讯作者：
Yuan, D.-Y.
作者机构：
[郭其强] Institute for Forest Resources & Environment of Guizhou, Guizhou University, Guiyang, Guizhou 550025, China;[杨瑞] College of Forestry, Guizhou University, Guiyang, Guizhou 550025, China;[袁德义] Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education and the Key Laboratory of Non-Wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha, Hunan 410004, China;[高超] Institute for Forest Resources & Environment of Guizhou, Guizhou University, Guiyang, Guizhou 550025, China, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education and the Key Laboratory of Non-Wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
通讯机构：
[Yuan, D.-Y.] K;Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education and the Key Laboratory of Non-Wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha, Hunan, China
关键词：
油茶;柱头;花柱;显微结构;超微结构
摘要：
[目的]从显微和超微层面探究油茶柱头和花柱的结构特征,可为探讨油茶自交不亲和性的细胞学机制提供理论依据,同时也为油茶杂交育种工作奠定基础。[方法]以开花当天的雌蕊为试验材料,利用细胞学方法研究油茶柱头和花柱的显微及超微结构特征。[结果]油茶雌蕊具完整的柱头、花柱和子房结构,湿性柱头其表面密布乳突细胞,排列紧密,细胞质浓厚,细胞器丰富,细胞间具有丰富的分泌物。基本组织细胞内含物和细胞器较少,细胞壁薄且壁外无分泌物。整个花柱由3 5根独立花柱组成,上端约2 /3部分相互分离形成离生区,从2 /3处开始到花柱基部相互连接形成合生区。每根花柱中空,由外表皮、基本组织和花柱道内表皮构成。花柱通道细胞外充满大量分泌物,该细胞比基本组织细胞大而规则、细胞质浓厚,具有腺质细胞的特点。花柱道从上到下贴合逐渐紧密,花柱基部的各花柱道之间相互连通,形成狭小的缝隙。[结论]本研究分析了油茶柱头和花柱的显微及超微结构特征,探明柱头乳突和花柱通道细胞为分泌型细胞,基本组织细胞结构单一,从柱头到子房花柱道逐渐变窄,在基部相互连通形成狭小的缝隙,花柱通道细胞从上至下壁外分泌物和细胞内细胞质浓度、细胞器数量与种类具有逐渐增加的趋势和特点。
语种：
中文
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Structural characteristics of the mature embryo sac of Camellia oleifera
作者：
Gao, Chao;Yang, Rui;Yuan, Deyi*
期刊：
Nordic Journal of Botany ,2018年36(6):njb-01673- ISSN：0107-055X
通讯作者：
Yuan, Deyi
作者机构：
[Gao, Chao] Guizhou Univ, Inst Forest Resources & Environm Guizhou, Guiyang, Guizhou, Peoples R China.;[Yang, Rui] Guizhou Univ, Coll Forestry, Guiyang, Guizhou, Peoples R China.;[Yuan, Deyi] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Hunan, Peoples R China.;[Yuan, Deyi] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod, Forestry Minist, Changsha, Hunan, Peoples R China.
通讯机构：
[Yuan, Deyi] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod, Forestry Minist, Changsha, Hunan, Peoples R China.
关键词：
ovule;embryo sac;female germ unit;three-dimensional structure;ultrastructure;confocal laser scanning microscope
摘要：
The ovule is the most important reproductive organ in the pistil of phanerogamae. Camellia oleifera (Theaceae) is an important woody plant producing edible oil in southern China, and its embryo sac structure has a positive effect on seed breeding. In this study, the microstructure, ultrastructure and three-dimensional structure of the ovule and embryo sac of C. oleifera were observed and described based on a combination of advanced microscopy techniques (SEM, TEM, CLSM). The ovule comprises the inner and outer integument. Large quantities of secretions in the micropylar canal exit and may participate in the guidance of the entry of the pollen tube into the embryo sac. The synergids have a dense cytoplasm, abundant organelles, and strong polarity. Little cytoplasm is present in the egg cell, yet there are many vacuoles. The center of the cell is taken up by a large vacuole, and the cytoplasm is pushed towards the edges to form obvious cytoplasmic cords. The two polar nuclei are large and conspicuous. The antipodal cells degenerate to fulfill a nutritional function.
语种：
英文
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Enhancing freezing tolerance of Brassica napus L. by overexpression of a stearoyl-acyl carrier protein desaturase gene (SAD) from Sapium sebiferum (L.) Roxb.
作者：
Peng, Dan;Zhou, Bo* ;Jiang, Yueqiao;Tan, XiaoFeng;Yuan, DeYi;...
期刊：
Plant Science ,2018年272:32-41 ISSN：0168-9452
通讯作者：
Zhou, Bo
作者机构：
[Zhou, Bo; Jiang, Yueqiao; Peng, Dan] Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Cent Cultivat & Utilizat Nonwo, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Peng, Dan] Forestry Biotechnol Hunan Key Labs, Changsha 410018, Hunan, Peoples R China.
通讯机构：
[Zhou, Bo] C;Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410018, Hunan, Peoples R China.
关键词：
Freezing tolerance;Sapium sebiferum (L.) Roxb.;Stearoyl-acyl carrier protein desaturase;Unsaturated fatty acid
摘要：
Sapium sebiferum (L.) Roxb. is an important woody oil tree and traditional herbal medicine in China. Stearoyl-acyl carrier protein desaturase (SAD) is a dehydrogenase enzyme that plays a key role in the transformation of saturated fatty acids into unsaturated fatty acids in oil; these fatty acids greatly influence the freezing tolerance of plants. However, it remains unclear whether freezing tolerance can be regulated by the expression level of SsSAD in S. sebiferum L. Our research indicated that SsSAD expression in S. sebiferum L. increased under freezing stress. To further confirm this result, we constructed a pEGAD-SsSAD vector and transformed it into B. napus L. W10 by Agrobacterium tumefaciens-mediated transformation. Transgenic plants that overexpressed the SsSAD gene exhibited significantly higher linoleic (18:2) and linolenic acid (18:3) content and advanced freezing tolerance. These results suggest that SsSAD overexpression in B. napus L. can increase the content of polyunsaturated fatty acids (PUFAs) such as linoleic (18:2) and linolenic acid (18:3), which are likely pivotal in improving freezing tolerance in B. napus L. plants. Thus, SsSAD overexpression could be useful in the production of freeze-tolerant varieties of B. napus L. © 2018 Elsevier B.V.
语种：
英文
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Increasing branch and seed yield through heterologous expression of the novel rice S-acyl transferase gene OsPAT15 in Brassica napus L.
作者：
Peng, Dan;Tan, Xiaofeng;Zhang, Lin;Yuan, Deyi;Lin, Jianzhong;...
期刊：
Breeding Science ,2018年68(3):326-335 ISSN：1344-7610
通讯作者：
Zhou, Bo
作者机构：
[Zhou, Bo; Jiang, Yueqiao; Peng, Dan] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Tree, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Cent Cultivat & Utilizat Nonwo, Changsha 410004, Hunan, Peoples R China.;[Zhou, Bo; Liu, Xuanming; Lin, Jianzhong] Hunan Univ, Key Lab Plant Funct Genom Dev & Regulat, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Zhou, Bo] C;[Zhou, Bo] H;[Zhou, Bo] F;Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;Hunan Univ, Key Lab Plant Funct Genom Dev & Regulat, Changsha 410082, Hunan, Peoples R China.
关键词：
branching;seed yield;OsPAT15;B. napus L.;sulfur acyl transferase
摘要：
Branching is a predominant element in the plant architecture of Brassica napus L. and represents an important determinant of seed yield. OsPAT15 (OsDHHC1), a novel DHHC-type zinc finger protein gene, was reported to regulate rice plant architecture by altering the tillering. However, whether heterologous overexpression of the OsPAT15 gene from the monocot rice into the dicot B. napus L. would have the same effect on branching or seed yield is unknown. In this study, the DHHC-type zinc finger protein gene OsPAT15 was determined to have sulfur acyl transferase activity in the akr1Δ yeast mutant in a complementation experiment. Heterologously expressing OsPAT15 transgenic B. napus L. plants were obtained using the Agrobacterium-mediated floral-dip transformation method. As anticipated, OsPAT15 transgenic plants exhibited branching and seed yield. Compared with non-transgenic plants, OsPAT15 transgenic plants had increased primary branches (1.58–1.76-fold) and siliques (1.86–1.89-fold), resulting in a significant increase in seed yield (around 2.39– 2.51-fold). Therefore, overexpression of the sulfur acyl transferase gene OsPAT15 in B. napus L. could be used to increase seed yield and produce excellent varieties. © 2018, Japanese Society of Breeding. All rights reserved.
语种：
英文
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Characteristics of Developmental Differences between Fertile and Aborted Ovules in Camellia oleifera
作者：
Gao, Chao;Yang, Rui;Yuan, Deyi*
期刊：
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE ,2017年142(5):330-336 ISSN：0003-1062
通讯作者：
Yuan, Deyi
作者机构：
[Gao, Chao] Guizhou Univ, Inst Forest Resources & Environm Guizhou, Guiyang 550025, Guizhou, Peoples R China.;[Yang, Rui] Guizhou Univ, Coll Forestry, Guiyang 550025, Guizhou, Peoples R China.;[Yuan, Deyi] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod, Forestry Minist, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Yuan, Deyi] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod, Forestry Minist, Changsha 410004, Hunan, Peoples R China.
关键词：
abortion;embryo sac;embryology;plant reproductive biology;seed
摘要：
Camellia oleifera is an important woody edible oil plant in southern China. In this study, the developmental differences in ovules at different positions in the ovary of C. oleifera were observed. The developmental type and characteristics of aborted ovules, ratios of normal and aborted ovules, and their developmental differences after flowering were examined. Ovules near the stylar end and in the middle exhibit normal development and are able to form embryo sacs; lower ovules near the pedicel end are usually aborted. The proportion of abortion of four examined cultivars ranges from 10.2% to 33.3%. Aborted ovules can be divided into four categories: 1) nascent egg apparatus lacking distinguishable cells; 2) completely absent egg apparatus structure consisting of flocculent tissue; 3) lack of tissue, comprising only integument cells; and 4) the inner integument not constituting a micropyle channel, with incomplete egg apparatus development and generating abnormal ovules. At 120 days after pollination (DAP), significant distinguishable size differences were found between fertile and aborted ovules; aborted ovules ceased growth at 180 DAP. On fruit maturation, aborted seeds were still attached to the placenta. © 2017, American Society for Horticultural Science. All rights reserved.
语种：
英文
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修剪强度对锥栗叶片生理及产量的影响
作者：
王刚;袁德义;邹锋;熊欢;朱周俊;...
期刊：
植物生理与分子生物学学报 ,2017年53(2):264-272 ISSN：1671-3877
通讯作者：
Yuan, D.-Y.
作者机构：
[王刚; 袁德义; 邹锋; 熊欢; 朱周俊; 刘智强; 欧阳芬] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室, 长沙, 410004
通讯机构：
Key Laboratory of Cultivation and Protection for Non-wood Forest Trees of Ministry of Education, Central South University of Forestry and Technology, Changsha, China
关键词：
锥栗;修剪;叶片质量;光合特性;产量
摘要：
为探究锥栗树体合理的修剪强度,以7年生'华栗3号'锥栗为试材,通过轻修剪(15%修剪量)、中修剪(30%修剪量)、重修剪(45%修剪量)和不修剪(对照)处理,研究了不同修剪强度对锥栗叶片质量、矿质元素含量和光合作用以及产量的影响。结果表明,不同修剪强度对锥栗不同冠层叶片质量影响不同,其中中修剪综合效果最好,在冠层上部,其单叶面积最大为72.92 cm~2,比对照增加了18.38%;在树冠中部和下部,叶片厚度均显著大于轻修剪和对照处理;中修剪的冠层下部叶片SPAD值分别较重修剪、轻修剪和对照增加4.27%、8.05%和8.29%。中修剪处理显著提高了叶片中N、P、K和Ca含量,但中修剪与重修剪Ca含量差异不显著,重修剪叶片Ca含量最高为35.56 mg·g~(-1)。中修剪的冠层上部叶片的净光合速率为12.85 μmol·m~(-2)·s~(-1)、蒸腾速率为3.75 mmol·m~(-2)·s~(-1)和气孔导度为0.18 mmol·m~(-2)·s~(-1),都是最高,较对照分别增加了20.3%、12.3%和20.0%;重修剪冠层上部胞间CO_2浓度较对照处理降低了2.32%。锥栗不同修剪强度的光合参数差异显著,中修剪锥栗树体叶片的最大光合速率、光饱和点和羧化效率显著高于其他处理,而光补偿点和暗呼吸速率显著低于其他处理。中修剪处理锥栗单株产量最高为10.25 kg,分别较重修剪、轻修剪与对照提高了9.58%、10.96%和21.61%,而重修剪处理单果重最高为7.76 g。综上,中修剪较其他处理能显著提高锥栗叶片质量与光合能力及产量,为锥栗结果期适宜的修剪强度。
语种：
中文
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修剪强度对锥栗冠层光照分布与产量及品质的影响
作者：
王刚;袁德义;邹锋;熊欢;朱周俊;...
期刊：
果树学报 ,2017年34(3):329-336 ISSN：1009-9980
作者机构：
[王刚; 袁德义; 邹锋; 熊欢; 朱周俊; 刘智强; 欧阳芬] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室, 长沙, 410004
关键词：
锥栗;修剪;相对光照强度;产量;品质
摘要：
【目的】研究锥栗不同修剪强度的冠层内相对光照强度分布与果实产量及品质特点,探究锥栗结果期适宜的修剪强度。【方法】以7 a生'华栗3号'锥栗为试材,应用树冠分格方法,测定了锥栗不同修剪强度冠层内的相对光照强度分布、单株产量和果实品质等指标。【结果】重修剪、中修剪和轻修剪与对照相比均明显改善树冠内的光照条件,重修剪、中修剪、轻修剪冠层内相对光照强度大于30%的分别占86.11%、80.56%和75%;不同修剪强度处理中,中修剪单株产量最高,为10.25 kg,其次是轻修剪和重修剪,分别为9.29 kg和9.40 kg,而对照产量最少,为8.47 kg;重修剪在树冠中层和上层的单粒质量和淀粉含量显著高于轻修剪和对照,且含水量显著低于轻修剪和对照,中修剪和重修剪的可溶性糖和还原性糖含量显著高于轻修剪和对照。【结论】综合产量和品质两方面分析,中修剪为锥栗结果期较理想的修剪强度。
语种：
中文
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锥栗花序分化发育过程中的生理生化变化
作者：
范晓明;袁德义;李建中;田晓明;熊欢;...
期刊：
植物生理与分子生物学学报 ,2017年53(12):2206-2214 ISSN：1671-3877
通讯作者：
Yuan, D.-Y.
作者机构：
中南林业科技大学, 经济林培育与保护教育部重点实验室;[范晓明; 袁德义] 经济林育种与栽培国家林业局重点实验室, 长沙, 410004;江西黎川沙塘隘木材检查站, 江西, 抚州, 344611;湖南省森林植物园, 长沙, 410116;中南林业科技大学, 经济林育种与栽培国家林业局重点实验室, 长沙, 410004
通讯机构：
Key Laboratory of Non-Wood Forest Product of State Forestry Administration, Central South University of Forestry and Technology, Changsha, China
关键词：
锥栗;花序;性别分化;生理生化变化
摘要：
为了探讨锥栗花序分化发育的营养生理基础,为人工调控锥栗雌雄花比提供理论依据,以‘华栗4号'锥栗为试材,采用石蜡切片法和田间观测法,明确花序分化特点,对比分析锥栗两性花序和雄花序各时期生理生化特征。结果表明:锥栗雄花序具有芽内分化特点,分化发育时间长达11个月;而锥栗两性花序为芽外分化,分化时间集中在4~5月。锥栗两性花序可溶性糖含量、可溶性蛋白质含量和C/N在大多数时期均显著高于雄花序, SOD、POD和CAT活性在性别分化时期(时期II)均达到最高值, N、P、K、Ca、Mn和Ze含量与雄花序在大多数时期存在显著性差异。锥栗花序分化与物质能量代谢和矿质元素积累等生理生化因子密切相关,且相关因子之间存在协同或者拮抗作用;花性别分化时期是两性花序分化生理生化因子变化的关键时期;高水平的碳水化合物、可溶性蛋白、P、Ca、Zn以及C/N有利于锥栗两性花序的分化。
语种：
中文
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油茶胚囊观察体系优化及三维结构重建
作者：
高超;袁德义
期刊：
植物生理与分子生物学学报 ,2017年53(6):1104-1108 ISSN：1671-3877
通讯作者：
Yuan, D.-Y.
作者机构：
贵州大学, 贵州省森林资源与环境研究中心, 经济林培育与保护教育部重点实验室;[高超] 经济林育种与栽培国家林业局重点实验室, 贵阳, 550025;中南林业科技大学, 经济林培育与保护教育部重点实验室;[袁德义] 经济林育种与栽培国家林业局重点实验室, 长沙, 410004;[高超] 贵州大学, 贵州省森林资源与环境研究中心, 经济林培育与保护教育部重点实验室
通讯机构：
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education, The Key Laboratory of Non-Wood Forest Products of Forestry Ministry, Central South University of Forestry and Technology, Changsha, China
关键词：
油茶;胚囊;三维结构;激光共聚焦扫描显微镜
摘要：
筛选并利用优化的染色和透明实验方法,结合激光共聚焦扫描显微镜观察油茶成熟胚囊内部结构。结果表明,采用1%曙红染色1 h并用水杨酸甲酯透明5 d是观察油茶成熟胚囊较好的实验处理方案,胚囊内细胞轮廓清晰,对所需观察的目标有很好的表现力。基于该优化体系,构建了油茶胚囊的三维结构,为油茶及山茶属植物胚囊发育、受精机理等方面的研究提供了更加快速、高效的观察方法。
语种：
中文
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Comprehensive Transcriptome Analysis of Phytohormone Biosynthesis and Signaling Genes in the Flowers of Chinese Chinquapin (Castanea henryi)
作者：
Fan, Xiaoming;Yuan, Deyi* ;Tian, Xiaoming;Zhu, Zhoujun;Liu, Meilan;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2017年65(47):10332-10349 ISSN：0021-8561
通讯作者：
Yuan, Deyi;Cao, Heping
作者机构：
[Zhu, Zhoujun; Liu, Meilan; Yuan, Deyi; Fan, Xiaoming] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;[Zhu, Zhoujun; Liu, Meilan; Yuan, Deyi; Fan, Xiaoming] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Hunan, Peoples R China.;[Tian, Xiaoming] Hunan Forest Bot Garden, Changsha 410116, Hunan, Peoples R China.;[Cao, Heping] ARS, USDA, Southern Reg Res Ctr, New Orleans, LA 70124 USA.
通讯机构：
[Yuan, Deyi] C;[Cao, Heping] A;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Hunan, Peoples R China.;ARS, USDA, Southern Reg Res Ctr, New Orleans, LA 70124 USA.
关键词：
Castanea Miller;flower development;hormone;RNA-Seq;sex differentiation;transcriptome
摘要：
Chinese chinquapin (Castanea henryi) nut provides a rich source of starch and nutrients as food and feed, but its yield is restricted by a low ratio of female to male flowers. Little is known about the developmental programs underlying sex differentiation of the flowers. To investigate the involvement of phytohormones during sex differentiation, we described the morphology of male and female floral organs and the cytology of flower sex differentiation, analyzed endogenous levels of indole-3-acetic acid (IAA), gibberellins (GAs), cytokinins (CKs), and abscisic acid (ABA) in the flowers, investigated the effects of exogenous hormones on flower development, and evaluated the expression profiles of genes related to biosyntheses and signaling pathways of these four hormones using RNA-Seq combined with qPCR. Morphological results showed that the flowers consisted of unisexual and bisexual catkins, and could be divided into four developmental stages. HPLC results showed that CK accumulated much more in the female flowers than that in the male flowers, GA and ABA showed the opposite results, while IAA did not show a tendency. The effects of exogenous hormones on sex differentiation were consistent with those of endogenous hormones. RNA-Seq combined with qPCR analyses suggest that several genes may play key roles in hormone biosynthesis and sex differentiation. This study presents the first comprehensive report of phytohormone biosynthesis and signaling during sex differentiation of C. henryi, which should provide a foundation for further mechanistic studies of sex differentiation in Castanea Miller species and other nonmodel plants.<br/> ©2017 American Chemical Society.
语种：
英文
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Flower Bud Differentiation and Development of ‘Jinsi No.4’ Jujube ( Mill.) in Hunan Province of Southern China
作者：
Feng Zou;Jinghua Duan;Huan Xiong;Deyi Yuan;Lin Zhang;...
期刊：
The Open Biotechnology Journal ,2017年11(1):9 -15 ISSN：1874-0707
通讯作者：
Yuan, D.
作者机构：
The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan 410004, China;Chinese Academy of Forestry, Beijing, 100091, China;Texas A&M AgriLife Research Center at El Paso, Texas A&M University System, 1380 A&M Circle, El Paso, TX 79927, United States
通讯机构：
The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan, China
关键词：
Bud differentiation;Floral organ;fruit crop;Ziziphus jujuba ‘Jinsi No.4’.
摘要：
Ziziphus jujuba Mill. is one of the most important fruit crops and has been cultivated in China for more than 4000 years. Z. jujuba fruit is rich in nutritional and medicinal values. Compared to other wood fruits, Z. jujuba is unique in its flowering and fruiting characteristics. Floral buds differentiation and formation of Z. jujuba is an essential process that affects yield. Z. jujuba ‘Jinsi No.4' blooms profusely, yet its final yield is low. In this study, the floral bud differentiation and development of ‘Jinsi No.4' were examined by paraffin section. Results showed that the floral buds of ‘Jinsi No.4' differentiated in the current year and started from early April. The duration of a single flower differentiation was short, taking only 7 days for maturation of flowers buds. Floral bud differentiation of Jinsi No.4' can be divided into six stages, i.e., pre-differentiation, initial differentiation, sepal differentiation, petal differentiation, stamen differentiation, and pistil differentiation. Flower development experienced seven stages, i.e., alabastrum, alabastrum break, sepal flattening, petal flattening, stamen flattening, filament withering, and ovule swelling. Dysplasia was observed in some floral organs in Z. jujuba Jinsi No.4', suggesting that the dysplasia of floral organs may be one of the main reasons for low yields. Our findings on flower bud development in ‘Jinsi No.4' will contribute to its production and flowering management in Hunan area of southern China. © 2017 Zou et al.
语种：
英文
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海南油茶5个无性系光合特性比较研究
作者：
袁军;袁德义;谭晓风;邹锋;肖诗鑫
期刊：
中南林业科技大学学报 ,2017年37(5):1-6 ISSN：1673-923X
作者机构：
[袁军; 袁德义; 谭晓风; 邹锋; 肖诗鑫] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室, 湖南, 长沙, 410004
关键词：
油茶;净光合速率;光合特性;日变化
摘要：
为了研究海南油茶光合效率的高低及不同无性系之间光合特性的差异,筛选高光效的海南油茶无性系,以初步筛选的5个油茶优良无性系‘海南2号’、‘海南6号’、‘海南9号’、‘海南18号’和‘海南19号’为试材,利用Li-6400xt便携式光合作用测定系统,测定了其光合作用日变化及光合-光响应曲线和光合-CO_2响应曲线,结果表明:‘海南2号’的净光合速率日变化呈单峰曲线,其它4个油茶无性系的净光合速率日变化呈双峰曲线;‘海南18号’的蒸腾速率呈单峰曲线,其它4个油茶无性系的蒸腾速率呈双峰曲线,峰值分别出现在9:00和15:00; ‘海南18号’和‘海南2号’气孔导度的日变化呈单峰曲线,‘海南9号’、‘海南19号’及‘海南6号’的日变化与P_n的日变化相一致;‘海南2号’的光饱和点和光补偿点最低,'海南19号’的光饱和点和光补偿点最高;5个油茶无性系的CO_2补偿点较高,具有C_3植物的光合特性;5个无性系P_n日变化的平均值由高到低顺序为‘海南19号,> ‘海南18号,> ‘海南9号,> ‘海南2号,〉‘海南6号,;相关分析表明,影响P_n的主要生理生态指标大小顺序依次为气孔导度 > 光合有效辐射〉蒸腾速率〉胞间CO_2浓度。
语种：
中文
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Increasing seed oil content and altering oil quality of Brassica napus L. by over-expression of diacylglycerol acyltransferase 1 (SsDGAT1) from Sapium sebiferum (L.) Roxb.
作者：
Peng, Dan;Zhang, Lin;Tan, Xiaofeng;Yuan, Deyi;Liu, Xuanming;...
期刊：
Molecular Breeding ,2016年36(10):1-14 ISSN：1380-3743
通讯作者：
Zhou, Bo
作者机构：
[Zhou, Bo; Peng, Dan] Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Cent Cultivat & Utilizat Nonwo, Changsha 410018, Hunan, Peoples R China.;[Liu, Xuanming] Hunan Univ, Key Lab Plant Funct Genom Dev & Regulat, Changsha 410082, Hunan, Peoples R China.;[Zhou, Bo; Peng, Dan] Forestry Biotechnol Hunan Key Labs, Changsha 410018, Hunan, Peoples R China.
通讯机构：
[Zhou, Bo] F;Forestry Biotechnol Hunan Key Labs, Changsha 410018, Hunan, Peoples R China.
关键词：
Sapium sebiferum L.;SsDGAT1;Clone;Function;Brassica napus L.;Oleic acid content
摘要：
Sapium sebiferum (L.) Roxb. [S. sebiferum] is one of the most important woody oil trees and traditional herbal medicines in China. Diacylglycerol acyltransferases (DGATs) esterify sn-1,2-diacylglycerol with a long-chain fatty acyl-CoA acting as a key enzyme at the last and rate-limiting step of triacylglycerol (TAG) biosynthesis in eukaryotic organisms. Although at least 61 DGAT1 sequences from 48 organisms have been identified, until now there have been almost no reports on the DGAT1 gene in S. sebiferum (SsDGAT1). In an attempt to clarify SsDGAT1’s function, we cloned the CDS (coding sequence) of SsDGAT1 by RACE (rapid amplification of cDNA ends) technology. The full-length CDS of SsDGAT1 contains 1524 bp, encoding a protein of 507 amino acids. Even recombinant SsDGAT1 was able to restore TAG biosynthesis in the yeast strain S. cerevisiae H1246 TAG-deficient mutant. Moreover, when SsDGAT1 was placed under the control of 35S promoter in Brassica napus L. W10, the total oil content of transgenic rape plants showed an increase of 12.3–14.7 % compared with the wild type. In addition, the transgenic rapeseed with heterologous expression of SsDGAT1 showed an alteration in seed oil composition overall: a significant decrease in oleic acid levels but a tendency towards an increase in linolenic acid levels. The result confirmed that SsDGAT1 may be involved in flux control of oil biosynthesis and could be used specifically to manipulate and improve oil content and composition in plants. These experimental findings suggest that we might be able to develop a plant high in industrial oils by over-expression of SsDGAT1 in S. sebiferum. © 2016, Springer Science+Business Media Dordrecht.
语种：
英文
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海南油茶优良单株果实经济性状综合评价与筛选
作者：
王碧芳;邹锋;袁德义;袁军;肖诗鑫;...
期刊：
福建农林大学学报(自然科学版) ,2016年45(2):156-161 ISSN：1671-5470
作者机构：
[王碧芳; 邹锋; 袁德义; 袁军; 肖诗鑫; 高超; 李泽; 彭小博] 中南林业科技大学林学院, 经济林培育与保护湖南省省部共建教育部重点实验室, 湖南, 长沙, 410004
关键词：
海南油茶;优良单株;果实经济性状;主成分分析
摘要：
对海南50个不同油茶优良单株的果实和大陆油茶‘华金’(对照)的果实经济性状指标进行测定,并采用SPSS19.0软件对其进行主成分分析.结果表明:各优株及其对照的果实经济性状的变异程度显著不同,变异系数6.73%~46.92%;用主成分抽提出5个特征值>1的主成分,累积贡献率为81.844%;二维排序图揭示了不同优良单株前3个主成分(油品质因子、果实大小因子和出籽率因子)的分布情况;50份海南油茶果实经济性状综合评价排名前10位分别为:TCPX-7、CMJL-6、QZWL-3、CMJL-4、QZCZ-3、QZCZ-4、CMZX-1、TCPX-3、QZWL-2和TCNK-1,而对照‘华金’排名21名.本研究找出了海南油茶果实经济性状评价的5个主成分,揭示了海南油茶不同优良单株的茶油品质差异.
语种：
中文
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Overexpression of SsDGAT2 from Sapium sebiferum (L.) Roxb Increases Seed Oleic Acid Level in Arabidopsis
作者：
Wang, YiQiang;Peng, Dan;Zhang, Lin;Tan, XiaoFeng;Yuan, DeYi;...
期刊：
Plant Molecular Biology Reporter ,2016年34(3):638-648 ISSN：0735-9640
通讯作者：
Zhou, Bo
作者机构：
[Zhou, Bo; Wang, YiQiang; Peng, Dan] Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410004, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Peng, Dan; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Peng, Dan; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Tree, Changsha 410004, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Ctr Cultivat & Utilizat Nonwoo, Changsha 410004, Hunan, Peoples R China.;[Liu, XuanMing] Hunan Univ, Key Lab Plant Funct Gnomon Dev & Regulat, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Zhou, Bo] C;Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Tree, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Collaborat Innovat Ctr Cultivat & Utilizat Nonwoo, Changsha 410004, Hunan, Peoples R China.
关键词：
S. sebiferum (L.) Roxb;SsDGAT2;Clone;Function;Arabidopsis;Oleic acid content
摘要：
Sapium sebiferum (L.) Roxb is one of the most important oil trees in China. Diacylglycerol acyltransferases (DGATs) esterify sn-1, 2-diacylglycerol with a long-chain fatty acyl-CoA, the last step and the rate-limiting step of triacylglycerol (TAG) biosynthesis in prokaryotic and eukaryotic organisms. At least 74 DGAT2 sequences from 61 organisms have been identified, but the SsDGAT2 gene had not been reported to date. To clarify the function of SsDGAT2, we cloned the CDS (rapid amplification of cDNA end) of SsDGAT2 by RACE technology. The full-length CDS of SsDGAT2 contains 1011 bp and encodes a protein of 336 amino acids. Recombinant SsDGAT2 restored TAG biosynthesis to the yeast strain Saccharomyces cerevisiae H1246 TAG-deficient mutant and preferentially incorporated unsaturated C18 fatty acids into lipids. To investigate the biotechnological potential of SsDGAT2, it was expressed under the control of the 35S promoter in Arabidopsis Col-4. The oleic acid content increased by 50 % in transgenic plants relative to the control. The results indicated that most of the oleic acid increase was at the expense of linolenic acid (18:3) content, which suggests that high-oleic-acid-content seeds can be created by the overexpression of SsDGAT2 in S. sebiferum (L.) Roxb.
语种：
英文
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锥栗芽苗砧嫁接技术
作者：
谭露曼;袁德义;张党权;熊欢;刘冬明;...
期刊：
经济林研究 ,2016年34(3):153-157 ISSN：1003-8981
作者机构：
中南林业科技大学林学院;中南林业科技大学经济林育种与栽培国家林业局重点实验室;中南林业科技大学经济林培育与保护省部共建教育部重点实验室;中南林业科技大学经济林培育与利用湖南省2011协同创新中心;[朱周俊; 袁德义; 谭露曼; 熊欢; 张旭辉; 刘冬明; 张党权] 中南林业科技大学
关键词：
锥栗;芽苗砧嫁接;嫁接时期;砧木年龄;封口材料与封口方式
摘要：
为了提高锥栗芽苗砧嫁接成活率，以野生锥栗种子作为砧木，以‘华栗3号’当年生营养枝作为接穗，采用嫩枝劈接法，研究4个嫁接时期（4月20日、5月10日、6月1日、7月10日）、5种日龄砧木（20、30、45、60、90 d）和封口材料及封口方式（铝箔、嫁接膜封顶、嫁接膜不封顶）对锥栗芽苗砧嫁接成活率的影响。结果表明：封口材料与封口方式对嫁接成活率存在较大影响，不同的封口材料与封口方式处理的嫁接苗成活率从高到低依次为：嫁接膜封顶、铝箔、嫁接膜不封顶；6月1日为最佳嫁接时期，嫁接膜封顶处理的嫁接成活率达64.00%；砧木年龄过小（20 d）或过大（90 d）均对嫁接成活不利，当砧木日龄为45 d 时，嫁接成活率最高，为69.33%。
语种：
中文
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锥栗花粉直感效应研究
作者：
张旭辉;袁德义;邹锋;范晓明;唐静;...
期刊：
园艺学报 ,2016年43(1):61-70 ISSN：0513-353X
通讯作者：
Yuan, D.-Y.
作者机构：
[张旭辉; 袁德义; 邹锋; 范晓明; 唐静; 朱周俊] 中南林业科技大学, 经济林育种与栽培国家林业局重点实验室;[张旭辉; 袁德义; 邹锋; 范晓明; 唐静; 朱周俊] 经济林培育与保护教育部重点实验室, 长沙, 410004
通讯机构：
[Yuan, D.-Y.] K;Key Laboratory of Non-Wood Forest Product of State Forestry Administration, China
关键词：
锥栗;授粉;坐果率;花粉直感;平均隶属度法
摘要：
为研究锥栗花粉直感效应,选用‘华栗1号'、‘华栗2号'、‘华栗3号'和‘黄榛'4个品种为试材,进行自交、异交授粉试验,以自然授粉作为对照,共20个授粉组合,分析不同授粉组合的果实成熟期、结实率及果实品质等,结果表明:锥栗在果实成熟期、坐果率、结实率、果实大小、可溶性糖、脂肪、蛋白质、直链淀粉和维生素C等方面表现明显的花粉直感效应,而在出籽率、总淀粉含量和含水量等方面无明显花粉直感效应;通过平均隶属函数法分析20个授粉组合,‘华栗2号'ב华栗3号'得分最高,‘黄榛'自交得分最低。因此,‘华栗2号'ב华栗3号'为首选授粉组合。
语种：
中文
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硼锌微肥施用下枣果实综合品质的评价
作者：
韩志强;袁德义;陈文涛;李承想;谢凯瑶
期刊：
南京林业大学学报(自然科学版) ,2015年39(2):40-46 ISSN：1000-2006
作者机构：
[袁德义; 陈文涛; 韩志强; 谢凯瑶; 李承想] 中南林业科技大学
关键词：
枣;硼锌微肥;果实品质;因子分析;聚类分析
摘要：
为了对硼锌微肥单施及配施下枣(Ziziphus jujuba Mill.)果实综合品质进行评价,并确定适宜的施用量,以'金丝4号'枣果实为试材,通过硼锌微肥单施及配施共16种处理,将各处理下果实品质性状指标数据通过隶属函数转化后进行因子分析与聚类分析。结果表明:①隶属函数法转化后的数据经因子分析后,抽取出5个特征根>1的公因子,累计贡献率为80. 559%,将因子得分与相应权重之积累加得到综合分值并进行排序可知,适当施用硼锌微肥可以提高枣果实的综合品质,硼锌微肥单施或者配施的量较大对枣果实综合品质的提高作用不显著甚至会降低枣果实综合品质。②利用聚类分析将果实品质数据进行聚类可知,当硼肥单施时,以22. 5 kg/ hm~2标准按穴均施对枣果实综合品质的提高作用显著,减少或者增加用量对枣果实综合品质没有明显影响;当锌肥单施时,以不大于22. 5 kg/ hm~2标准按穴均施对枣果实综合品质的提高作用显著;硼锌微肥配施时,以15 kg/ hm~2或者22. 5 kg/ hm~2标准等量按穴均施对枣果实品质的提高作用最为显著。③硼锌微肥以15 kg/ hm~2标准按1∶1比例配施对枣果实综合品质的提高作用最明显,并可以最大限度地减少施用量。
语种：
中文
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A cytological study of anther and pollen development in Camellia oleifera
作者：
Gao, C.;Yuan, D. Y.* ;Wang, B. F.;Yang, Y.;Liu, D. M.;...
期刊：
Genetics and Molecular Research ,2015年14(3):8755-8765 ISSN：1676-5680
通讯作者：
Yuan, D. Y.
作者机构：
[Yang, Y.; Liu, D. M.; Gao, C.; Wang, B. F.; Yuan, D. Y.; Han, Z. Q.] Cent South Univ Forestry & Technol, Forestry Minist, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ,Key Lab Nonwood Forest Prod, Changsha, Hunan, Peoples R China.
通讯机构：
[Yuan, D. Y.] C;Cent South Univ Forestry & Technol, Forestry Minist, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ,Key Lab Nonwood Forest Prod, Changsha, Hunan, Peoples R China.
关键词：
Angiosperms;Camellia oleifera;Male gametophyte;Microsporogenesis;Pollen;Reproduction
摘要：
The plant species Camellia oleifera is an important producer of edible oil in China. However, it suffers from a low fruit-setting rate. This study used high resolution scanning electron microscopy of semi-thin tissue sections to investigate anther development and pollen formation, and to determine whether problems in forming functional pollen cause low seed setting rates. During anther development, cell sizes within the epidermis and end othecium gradually increase, and at the beginning of the microsporocyte phase, the cells become highly vacuolated. The cell walls of the inner wall cells thicken in a ribbon pattern during the later part of the two-cell pollen stage. Middle layer cells become flattened during the later part of the secondary sporogenous cell stage. A tapetum also forms at this stage; the secondary sporogenous cells begin to degrade at the early microspore stage and completely disappear by the time the pollen is mature, thus forming a glandular tapetum. In microsporocyte, cytokinesis occurs simultaneously with microsporocyte meiosis, and a tetrahedral arrangement is present in tetrads. Reproductive cells begin to form at the later stage of microsporogenesis; the nuclei of the reproductive cells are enlarged as the reproductive cells begin to move away from the pollen wall. Two-cell pollen is first formed after reproductive cells have left the pollen wall, and the number of inclusions in the pollen subsequently increases. The pollen matures for 5-8 days before flowering occurs. No pollen abortion phenomena were observed, suggesting that anther development in C. oleifera is normal and that functional pollen are formed. © FUNPEC-RP.
语种：
英文
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生草栽培对南方鲜食枣园土壤理化性质的影响
作者：
李承想;袁德义;韩志强;张琳;陈文涛
期刊：
经济林研究 ,2015年(4):70-74 ISSN：1003-8981
作者机构：
中南林业科技大学 经济林育种与栽培国家林业局重点实验室，湖南长沙 410004;中南林业科技大学 经济林培育与保护省部共建教育部重点实验室，湖南长沙 410004;中南林业科技大学 经济林培育与利用湖南省2011协同创新中心，湖南长沙 410004;[袁德义; 陈文涛; 韩志强; 张琳; 李承想] 中南林业科技大学
关键词：
生草栽培;鲜食枣园;土壤;理化性质
摘要：
为探寻适宜南方鲜食枣园生草栽培的草种，以白三叶、1年生黑麦草和多年生黑麦草为试材，在鲜食枣园分区分别进行生草栽培试验，通过与清耕区的对比，研究不同处理对土壤理化性质的影响。结果表明：①白三叶生草栽培条件下枣园0～15、15～30、30～45 cm的土壤含水量分别比对照高出37.5%、22.4%、15.6%；夏季白三叶生草栽培区地表和20 cm土层的温度分别比对照降低了4.8和6.4℃，降温保墒效果优于其它处理。②与对照相比，白三叶生草栽培区的土壤容重降低，土壤总孔隙度大幅增加，各处理的土壤总孔隙度由高到低依次为白三叶、多年生黑麦草、1年生黑麦草、对照；③生草区0～15 cm土层土壤养分含量都比对照高，各处理土壤有机质、全磷、全钾、速效氮含量增加幅度由高到低依次为白三叶、多年生黑麦草、1年生黑麦草、对照，各处理土壤全氮、速效磷、速效钾含量增幅由高到低依次为白三叶、1年生黑麦草、多年生黑麦草、对照。
语种：
中文
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