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Overexpression of SsDGAT2 from Sapium sebiferum (L.) Roxb Increases Seed Oleic Acid Level in Arabidopsis

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成果类型:
期刊论文
作者:
Wang, YiQiang;Peng, Dan;Zhang, Lin;Tan, XiaoFeng;Yuan, DeYi;...
通讯作者:
Zhou, Bo
作者机构:
[Zhou, Bo; Wang, YiQiang; Peng, Dan] Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410004, Hunan, Peoples R China.
[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Peng, Dan; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.
[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Peng, Dan; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Tree, Changsha 410004, Hunan, Peoples R China.
[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Ctr Cultivat & Utilizat Nonwoo, Changsha 410004, Hunan, Peoples R China.
[Liu, XuanMing] Hunan Univ, Key Lab Plant Funct Gnomon Dev & Regulat, Changsha 410082, Hunan, Peoples R China.
通讯机构:
[Zhou, Bo] C
Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410004, Hunan, Peoples R China.
Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.
Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Tree, Changsha 410004, Hunan, Peoples R China.
Cent South Univ Forestry & Technol, Collaborat Innovat Ctr Cultivat & Utilizat Nonwoo, Changsha 410004, Hunan, Peoples R China.
语种:
英文
关键词:
S. sebiferum (L.) Roxb;SsDGAT2;Clone;Function;Arabidopsis;Oleic acid content
期刊:
Plant Molecular Biology Reporter
ISSN:
0735-9640
年:
2016
卷:
34
期:
3
页码:
638-648
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31200516]; Outstanding Youth Scientific Research Foundation by the Hunan Education Department [13B150]; Hunan Province Outstanding Youth Teacher Cultivation Project [2014gg04]; Youth Scientific Research Foundation of Central South University of Forestry Technology [QJ2011046B]; Foundation of the Project for Talents Introduction of the Central South University of Forestry Technology [104-0182]
机构署名:
本校为第一且通讯机构
院系归属:
林学院
生命科学与技术学院
摘要:
Sapium sebiferum (L.) Roxb is one of the most important oil trees in China. Diacylglycerol acyltransferases (DGATs) esterify sn-1, 2-diacylglycerol with a long-chain fatty acyl-CoA, the last step and the rate-limiting step of triacylglycerol (TAG) biosynthesis in prokaryotic and eukaryotic organisms. At least 74 DGAT2 sequences from 61 organisms have been identified, but the SsDGAT2 gene had not been reported to date. To clarify the function of SsDGAT2, we cloned the CDS (rapid amplification of cDNA end) of SsDGAT2 by RACE technology. The full-length CDS of SsDGAT2 contains 1011 bp and encodes...

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