Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education, The Key Laboratory of Non-Wood Forest Products of Forestry Ministry, Central South University of Forestry and Technology, Changsha, China
The internal structure of mature embryo sac in Camellia oleifera was observed using confocal laser scanning microscope, based on choosing and utilizing the optimized staining and clearing experimental methods. The experiment methods, the samples were stained with 1% eosin solution for 1 h and treated with methyl salicylate transparent material for 5 d for embryo sac observation, was better than others. The embryo sac clearly showde cell configuration. According to the optimized experimental methods, the three-dimentional structure of C. oleifera embryo sac was reconstructed for the first time....
