作者： Yang, Mi;Hu, Xiao;Ouyang, Xiang;Sajjad, Muhammad;Ma, Xiaoling*;...

期刊： Brazilian Journal of Botany,2021年44(2):391-400 ISSN：0100-8404

通讯作者： Ma, Xiaoling;Yuan, Deyi

作者机构： [Ma, Xiaoling; Yuan, Deyi; Yang, Mi; Ma, XL; Hu, Xiao] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Peoples R China.;[Ma, Xiaoling; Yuan, Deyi; Yang, Mi; Hu, Xiao] South Univ Forestry & Technol, Forestry Minist, Key Lab Nonwood Forest Prod, Changsha 410004, Peoples R China.;[Ouyang, Xiang] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China.;[Sajjad, Muhammad] COMSATS Univ Islamabad CUI, Dept Biosci, Pk Rd, Islamabad 45550, Pakistan.

通讯机构： [Ma, XL; Yuan, DY] C;[Yuan, Deyi] S;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Peoples R China.;South Univ Forestry & Technol, Forestry Minist, Key Lab Nonwood Forest Prod, Changsha 410004, Peoples R China.

关键词： CoIDL gene family;Expression analysis;Identification

摘要： The INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) controls floral organ abscission in plants. IDA belongs to IDA-LIKE (IDL) gene family that is involved in regulation of Arabidopsis development. Herein, we identified three genes, CoIDA1, CoIDA2 and CoIDA3 in Camellia oleifera (Camellia oleifera Abel. cv. Huashuo) and suggested their involvement in the regulation of fruits abscission. The full-length cDNA sequences of CoIDA1, CoIDA2 and CoIDA3 were of 207 bp, 276 bp and 273 bp, encoding proteins of 68, 91 and 90 amino acids, respectively. These CoIDA genes were single exon genes (SEGs) with a conserved extended PIP motif (EPIP) at C-terminal that has been implicated to play an important role in governing protein function for enhanced flower abortion rate. The highest expression of CoIDA1 was in young peduncles and the lowest in young fruits. However, the highest expressions of CoIDA2 and CoIDA3 were both in young roots, and the lowest in young fruits. The expressions of CoIDA1 and CoIDA2 significantly increased in abscission zones (AZs) of both abnormal fruits (AF) and ethephon treated fruits (ETH-F) with respect to normal fruits (NF), which suggest that CoIDA1 and CoIDA2 genes are related to fruits abscission in C. oleifera. This study provided a preliminary understanding about CoIDA genes which could lead to their detailed functional analysis and utilization for improving C. oleifera yield potential.

语种： 英文

作者： 沈植国;孙萌;袁德义;程建明;丁鑫;...

期刊： 园艺学报,2021年47(12):2349-2361 ISSN：0513-353X

通讯作者： SHANG, Z.

作者机构： [袁德义] College of Forestry, Central South University of Forestry and Technology, Changsha, 410004, China;[尚忠海; 丁鑫; 孙萌] Henan Academy of Forestry, Zhengzhou, 450008, China;[程建明] Henan Kalefu Horticulture Limited Company, Zhengzhou, 450000, China;[沈植国] College of Forestry, Central South University of Forestry and Technology, Changsha, 410004, China, Henan Academy of Forestry, Zhengzhou, 450008, China

通讯机构： [SHANG, Z.] H;Henan Academy of ForestryChina

关键词： 蜡梅科;嫩梢;挥发性成分;顶空固相微萃取技术（HS–SPME）;气质联用（GC–MS）

摘要： 采用顶空固相微萃取(HS-SPME)与气质联用(GC-MS)分析技术,对蜡梅科6种植物挥发性成分进行检测,并通过共有成分分析、主成分分析、聚类分析,与药用价值较高的山蜡梅主要挥发性成分进行对比.检测结果表明:蜡梅'鸿运'(Chimonanthus praecox 'Hongyun')、'金玉'(Ch.praecox'Jinyu')和'鄢陵素心'(Ch.praecox 'Yanlingsuxin'),山蜡梅(Ch.nitens)、夏蜡梅(Calycanthus chinensis)及美国蜡梅(Ca.floridus)嫩梢挥发性成分种类分别为47、48、44、44、48和46种,共包含141种成分;蜡梅科6种植物共有挥发性成分以烯烃类、醇类物质为主,'鸿运''金玉''鄢陵素心'蜡梅、夏蜡梅和美国蜡梅与山蜡梅共有挥发成分分别为15、18、10、14和20种;PCA分析和聚类分析结果表明:夏蜡梅单独为一类,'鸿运''金玉''鄢陵素心'蜡梅为一类,山蜡梅和美国蜡梅为一类;'鸿运''金玉''鄢陵素心'蜡梅、夏蜡梅和美国蜡梅与山蜡梅的主要挥发性成分对比分析表明:桉叶油醇、对薄荷-1(7),3-二烯为主要差异成分.以上结果表明,6个蜡梅科植物资源嫩梢挥发性成分组成丰富,但种(品系)间存在一定的差异,均具有较大的药用开发潜力.

语种： 中文

作者： Li, Yanmin;Ye, Tianwen;Han, Chunxia;Ye, Zhihua;Zhang, Jian;...

期刊： Euphytica,2021年217(2):1-12 ISSN：0014-2336

通讯作者： Xiao, Shixin;Yuan, Deyi

作者机构： [Xiao, Shixin; Yuan, Deyi; Li, Yanmin; Ye, Tianwen; Han, Chunxia; Ye, Zhihua] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;[Zhang, Jian] Xinyang Agr & Forestry Univ, Xinyang 464000, Henan, Peoples R China.

通讯机构： [Xiao, SX; Yuan, DY] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.

关键词： Oil-tea camellia;Interspecific hybridization;Ploidy;Meiosis;Pollen

摘要： Oil-tea (Camellia oleifera) is an important woody plant species producing edible oil in southern China, which shows complicated cytogenetics and intraspecific polyploidy. In order to explore the ploidy variation in interspecific hybridization of oil-tea Camellia, the ploidy of ‘Huashuo’, ‘Youza 2’ and 18 superior hybrids of ‘Youza 2’ × ‘Huashuo’ were identified by flow cytometry and chromosome counting. C.oleifera variety ‘Huashuo’ was hexaploid (2n=90), and Camellia yuhsienensis superior plant ‘Youza 2’ was aneuploid based on hexaploid (2n =91). There are not only hexaploid but also aneuploid in the ‘Huashuo’ × ‘Youza 2’ hybrids. Among the offspring plants, there are 11 plants each with chromosome number of 90, 6 plants each with 91, and 1 plants each with 92. To elucidate the causes of ploidy variation in above findings, the pollen characteristics and chromosomal behavior in meiosis of ‘Huashuo’ were examined by microscopy. The chromosome pairing configuration of meiosis terminal stage of microsporocytes of ‘Huashuo’ was 43 II + 2 IV. In all stages of meiosis, numerous abnormal behaviors were found, such as precocious chromosome migration, free chromosomes, lagging chromosomes and micronucleus. The abnormal rates of metaphase I, anaphase I, metaphase II and anaphase II were 67.5%, 88.1%, 58.4% and 78.9%, respectively. In the tetrad stage, abnormal phenomena such as dyads, triads, hexads and micronucleus were observed, with the frequency of 1.4%, 1.0%, 1.0% and 4.2%, respectively. The pollen diameter of ‘Huashuo’ varied widely (16.92–79.13μm), suggesting a wide range of ploidy level of pollen grains. From pollen diameter distribution, we concluded that ‘Huashuo’ produced a small number of unreduced pollen grains. These findings are valuable for cytogenetics research and selection strategy of ploidy breeding in C. oleifera. © 2021, The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature.

语种： 英文

作者： Xiong, Huan;Yuan, De-Yi;Chen, Ya;Niu, Genhua;Zou, Feng*

期刊： BANGLADESH JOURNAL OF BOTANY,2020年49(1):39-46 ISSN：0253-5416

通讯作者： Zou, Feng

作者机构： [Xiong, Huan; Yuan, De-Yi; Chen, Ya; Niu, Genhua; Zou, Feng] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod Forestry Minist, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zou, Feng] C;Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod Forestry Minist, Changsha 410004, Hunan, Peoples R China.

关键词： Chestnut;Colpus length;Equatorial width;Pollen axis length;Pollen morphology

摘要： The morphological characteristics of pollen grains of 16 cultivars belonging to two species of the genus Castanea (Castanea mollissima and C. henryi) collected from southern China were observed by using scanning electron microscope (SEM). The length of polar axis (PL), the equatorial diameter (ED), the length of colpus (CL) of grain, P/E ratio (polar axis to equatorial diameter), P/C ratio (polar axis to colpus length), and C/E ratio (colpus length to equatorial diameter) were measured and their variations were compared among studied taxa. The result of this study indicated that chestnut pollen grain was a tricolporate in aperture type, and aperprolate type in equatorial view. The morphological characteristics were mainly represented by PL, ED and CL as revealed by principal components analysis, which accounted for 99.56% of the total variations. The cluster analysis by the UPGMA tree divided the taxa into three groups and showed a great morphological affinity among the 16 cultivars. © 2020 Bangladesh Botanical Society. All rights reserved.

语种： 英文

作者： 无;袁德义;范晓明;郭素娟;陈辉;...

期刊： 中国科技成果,2020年(22):79-80 ISSN：1009-5659

作者机构： 中南林业科技大学;北京林业大学;中国林业科学研究院亚热带林业研究所;西北农林科技大学;福建农林大学

关键词： 木本粮食;铁杆庄稼;锥栗;高效生产;板栗;山区农民;我国林业

摘要： 板栗(C.mollissima Bl.)和锥栗(C.henryi Rehd.et Wils.)是我国重要的木本粮食树种,自古被视为"木本粮食""铁杆庄稼",在我国林业生产、经济建设和人们日常生活中占有十分重要的地位.目前我国有26个省(市)有栗栽培,栽培总面积2,700万亩,年产198.82万吨,占世界栗总产量的84%,全国栗从业人员达到500万人.栗已成为我国部分山区农民的重要收入来源,也是居民消费的重要木本粮食.

语种： 中文

作者： Gong, Wenfang;Song, Qiling;Ji, Ke;Gong, ShouFu;Wang, Lingkai;...

期刊： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2020年68(49):14670-14683 ISSN：0021-8561

通讯作者： Yuan, Deyi

作者机构： [Song, Qiling; Gong, Wenfang; Wang, Lingkai; Chen, Le; Yuan, Deyi; Ji, Ke; Gong, ShouFu] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;[Song, Qiling; Gong, Wenfang; Wang, Lingkai; Chen, Le; Yuan, Deyi; Ji, Ke; Gong, ShouFu] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod, Forestry Minist, Changsha 410004, Hunan, Peoples R China.;[Zhang, Jian] Xinyang Agr & Forestry Univ, Xinyang 464000, Henan, Peoples R China.

通讯机构： [Yuan, Deyi] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod, Forestry Minist, Changsha 410004, Hunan, Peoples R China.

关键词： Biochemistry;Biosynthesis;Fatty acids;Gas chromatography;Gene expression;Oils and fats;Proteins;RNA;Alternative splicing;Camellia oleifera seeds;Fatty acid metabolism;Gene expression patterns;Protein-protein interaction networks;Transcriptional factors;Transcriptome analysis;Weighted correlation;Petroleum prospecting;plant protein;transcriptome;vegetable oil;alternative RNA splicing;Camellia;chemistry;gene expression profiling;genetics;metabolism;plant seed;Alternative Splicing;Camellia;Gene Expression Profiling;Plant Oils;Plant Proteins;Seeds;Transcriptome

摘要： Camellia oleifera Abel., belonging to the genus Camellia of Theaceae, has been widely used as a cooking oil, lubricant, and in cosmetics. Because of complicated polyploidization and large genomes, reference genome information is still lacking. Systematic characterization of gene models based on transcriptome data is a fast and economical approach for C. oleifera. Pacific Biosciences single-molecule long-read isoform sequencing (Iso-Seq) and Illumina RNA-Seq combined with gas chromatography were performed for exploration of oil biosynthesis, accumulation, and comprehensive transcriptome analysis in C. oleifera seeds at five different developmental stages. We report the first full-length transcriptome data set of C. oleifera seeds comprising 40,143 deredundant high-quality isoforms. Among these isoforms, 37,982 were functionally annotated, and 271 (2.43%) belonged to fatty acid metabolism. A total of 8,344 full-length unique transcript models were obtained, and 8,151 (97.69%) of them produced more than two isoforms, suggesting a high degree of transcriptome complexity in C. oleifera seeds. A total of 783 alternative splicing (AS) events were identified, among which the retained intron was the most abundant. We also obtained 1,910 long noncoding RNAs (lncRNAs) and found that AS events occurred in these lncRNAs. Potential transcript variants of genes involved in oil biosynthesis were also investigated. After performing weighted correlation network analysis, we found seven ""gene modules""and hub genes for each module showing a significant association with oil content. The series test of clusters classified these modules into four significant profiles based on gene expression patterns. Protein-protein interaction network analysis showed that upregulated WRI1 interacted with 17 genes encoding the enzymes playing key roles in oil synthesis. MYB and ZIP transcriptional factors also showed significant interactions with key genes involved in oil synthesis. Collectively, our data advance the knowledge of RNA isoform diversity in seeds at different developmental stages and provide a rich resource for functional studies on oil synthesis in C. oleifera. © 2020 American Chemical Society.

语种： 英文

作者： Chen, Wangzun;He, Libing;Tian, Shiyi;Masabni, Joseph;Zhang, Riqing;...

期刊： Agronomy,2020年10(2):288- ISSN：2073-4395

通讯作者： Zou, Feng;Yuan, Deyi

作者机构： [Chen, Wangzun; He, Libing; Tian, Shiyi; Zhang, Riqing; Zou, Feng; Yuan, Deyi] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Peoples R China.;[Masabni, Joseph] Texas A&M Univ Syst, Texas A&M AgriLife Extens, 17360 Coit Rd, Dallas, TX 75252 USA.

通讯机构： [Zou, F; Yuan, DY] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Peoples R China.

关键词： chestnut burrs;cattle farming waste;waste-to-resource;composting;spicular structure rapid degradation;fluorescence parameters;plant biomass;circular economy;sustainable agriculture

摘要： In China, chestnut burrs (CB) are produced at a rate of a million tons per year as the major byproduct of chestnut orchards. It is necessary to utilize the chestnut forest green waste and convert it into a valuable seedling media for the sustainable cultivation of chestnut seedlings. In this study, we composted CB with two waste products of cattle farming, namely cow manure (CM) and bovine bone (BM). We also evaluated the potential of CB compost application in chestnut forest sustainability. Results indicated that the best combination was the addition of 15% BM and 55% CM. This combination significantly improved the composting environment by increasing pH, enhancing phosphorus concentration and mineral elements such as Ca, Na, Mg and Zn, and shortened the composting period to 38 days. This combination also resulted in the highest content of citric acid-P (109.20 times than the control treatment) and the lowest content of NH4 +–N (0.28 times than control treatment) indicating a better N and P structure of the final compost product. This combination achieved a greater degradation rate of CB cellulose (61.45%), hemicellulose (37.87%), and a more significant degradation of outer epidermis structure. When CB compost was used as a growing media, a significant decrease in photosynthesis stress of chestnut seedlings was observed, which was mainly manifested as a decrease in photochemical quenching (qP) and an increase of the maximum efficiency of PSII photochemistry under dark-adaption (Fv/Fm). Addition of 10% CB compost (in volume basis) is suggested, which resulted in the tallest chestnut seedlings (59.83 cm) with a stem diameter of 0.91 cm after six months of growth. In summary, this research provides an environmentally friendly strategy for chestnut orchard sustainability: rapid composting of CB, then immediate application as a high-quality substrate for chestnut seedlings. © 2020 by the authors.

语种： 英文

作者： Zou Feng;Zhang Xu-hui;Yuan De-yi*;Zhu Zhou-jun;Tan Lu-man;...

期刊： 光谱学与光谱分析,2019年39(1):286-291 ISSN：1000-0593

通讯作者： Yuan De-yi

作者机构： [Zou Feng; Tan Lu-man; Liu Dong-ming; Yuan De-yi; Zhang Xu-hui; Zhu Zhou-jun] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Yuan De-yi] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Hunan, Peoples R China.

关键词： 全自动间断化学分析仪;原子吸收光谱法;锥栗;花粉直感;矿质元素;主成分分析

摘要： 为探讨锥栗不同授粉组合果实中矿质元素是否也存在花粉直感效应, 应用全自动间断化学分析仪和原子吸收光谱法测定了“华栗1号”、 “华栗2号”、 “华栗3号”和“黄榛”四个锥栗主栽品种自交、 异交以及自然授粉的子代坚果中主要矿质元素的含量。 结果表明, 锥栗不同授粉组合坚果的N, P, K, Ca, Mg, Fe, Zn和Mn八种矿质元素含量差异较为显著, 其矿质元素具有明显的花粉直感效应, 尤其是铁、 锌元素受花粉直感作用的影响较大, “华栗2号”ד黄榛”授粉组合果实铁、 锌元素含量最高, 分别为162.13和41.79 μg·g-1; “黄榛”ד华栗1号”授粉组合果实中Mn元素含量最高为165.67 μg·g-1, 为锰微肥的利用提供了参考; 通过主成分分析, 评价出19个组合中在矿质元素方面表现最优的授粉组合为“华栗2号”ד黄榛”。 该研究结果可为锥栗生产上合理配置授粉树和改善果实品质提供科学的依据。 To elucidate the xenia effects of mineral elements on different pollination combinations in Castanea henryi, using the chinquapin cultivars “Huali No.1”, “Huali No.2”, “Huali No.3” and “Huangzhen” as materials, we investigated the xenia effects of mineral elements in C. henryi by auto dicsrete analyzers and atomic absorption spectrometry. Twenty combinations of self-, cross-, and natural pollination were undertaken. The results revealed that eight mineral elements of Castanea henryi seeds were significant differences. Xenia obviously has its mineral elements, especially iron and znic. The fruit of “Huali No.2”דHuangzhen” showed the highest iron and zinc content, which were 162.13 and 41.79 μg·g-1, respectively. The fruit of “Huangzhen”דHuali No.1” showed an increased manganese content of 165.67 μg·g-1, which provides a reference for the utilization of this variety as a manganese fertilizer.Through principal component analysis, the best combination was “Huali No.2”דHuangzhen” in the 19 combinations. The results can give a basis for planting design of varieties and improving fruit quality in C. henryi.

语种： 中文

作者： 李华威;王丽媛;孙鹏;索玉静;韩卫娟;...

期刊： 园艺学报,2019年46(10):1897-1906 ISSN：0513-353X

通讯作者： Yuan, D.

作者机构： [李华威; 袁德义] College of Forestry, Central South University of Forestry and Technology, Changsha, 410004, China;[王丽媛; 刁松锋; 韩卫娟; 买旖旎; 索玉静; 傅建敏; 孙鹏] Non-Timber Forest Research and Development Center, China Paulownia Research Center, State Forestry Administration, Chinese Academy of Forestry, Zhengzhou, 450003, China

通讯机构： [Yuan, D.] C;College of Forestry, China

关键词： 柿;雄全同株;雄花;两性花;雌蕊;雄蕊;形态学

摘要： 在龙岩野柿子代群体中发现一株雄全同株个体‘龙岩野柿1号’,全株3朵合生聚伞形花序的中间1朵为两性花的概率大于80％,两侧分别为完全雄花.以‘龙岩野柿1号’的雄花和两性花为材料,采用常规石蜡切片法对其雌、雄蕊发育进行观察.结果 表明:其两性花雌、雄蕊原基均发育正常,雄花雌蕊原基发育中无子房功能性组织产生;两性花发育早于雄花.雄花和两性花的花药4室,花药壁发育为双子叶型,由表皮、药室内壁、中层和腺质绒毡层组成;四分体为四面体型和对称型,减数分裂为同时型;成熟花粉为2-细胞型,3条萌发沟;单核靠边期存在少量畸形花粉粒和空花粉粒;不同药室及同一药室的小孢子发育存在不同步现象.两性花子房8室,中轴胎座,倒生胚珠,双珠被,薄珠心,单孢原细胞;大孢子四分体直线排列,功能大孢子位于合顶端,蓼型胚囊.

语种： 中文

作者： Xiong, Huan;Chen, Ping;Zhu, Zhoujun;Chen, Ya;Zou, Feng*;...

期刊： HORTSCIENCE,2019年54(7):1149-1155 ISSN：0018-5345

通讯作者： Zou, Feng;Yuan, Deyi

作者机构： [Xiong, Huan; Chen, Ping; Zhu, Zhoujun; Chen, Ya; Zou, Feng; Yuan, Deyi] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod State Forestry Adm, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zou, F; Yuan, DY] C;Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod State Forestry Adm, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.

关键词： Anther development;Anther vascular bundle;Cytoplasmic male sterility;Oil tea;Tapetum

摘要： Camellia oleifera is an important woody tree species in China that produces edible oil. Although sterile male C. oleifera plants play an important role in hybrid breeding, the possible cytological characteristics of pollen abortion remain unknown. To characterize the pollen abortion process, a genic petaloid-type sterile male C. oleifera ‘X1' plant was investigated using a cytological method. The results showed that in malefertile plants, the anthers were full and butterfly shaped, the pollen viability was as high as 97.5%, and the development of the tapetum and anther vascular bundles was normal. However, in male-sterile C. oleifera ‘X1', petaloidy in the anther was observed, and the pollen vitality was as low as 4.5%. Pollen abortion in sterile C. oleifera ‘X1' anthers occurred from the microspore stage to the mature pollen period. Further cytological analyses revealed an abnormally enlarged tapetum and retarded tapetum degeneration, suggesting that insufficient nutrients were provided for microspore development. Moreover, the anther vascular bundles displayed hyperplasia, and the pollen sac area became increasingly smaller, causing most anthers to be sterile and to have few pollen grains. Taken together, the results indicate that petaloid-type male sterility in C. oleifera may be attributed to abnormal development of the tapetum and anther vascular bundles. The findings clarify the pollen abortion period and the cytological characteristics of petaloid-type cytoplasmic male sterility in C. oleifera, and lay a solid foundation for the male sterile line in C. oleifera hybrid breeding. © 2019, American Society for Horticultural Science. All rights reserved.

语种： 英文

作者： 李艳丽;熊欢;彭小博;邹锋;袁德义

期刊： 西南农业学报,2019年32(4):792-797 ISSN：1001-4829

作者机构： [邹锋; 袁德义; 彭小博; 李艳丽; 熊欢] 中南林业科技大学

关键词： 锥栗;嫁接苗;实生苗;叶片解剖结构;光合特性

摘要： 【目的】以2年生锥栗嫁接苗与实生苗为试材,比较分析2种苗木在叶片生长状况、形态结构及光合特性上的差异,为锥栗的良种苗木繁育提供参考.【方法】本实验主要采用Li-6400便携式光合仪测定法和石蜡切片法.【结果】①嫁接苗与实生苗的净光合速率( P_(max) ) 、光补偿点( LCP) 、光饱和点( LSP) 、表现量子效率( AQY)及叶绿素( a + b)含量( C_c )等光合指标的差异显著( P < 0.05),嫁接苗的Cc为( 4.35 ± 0.47) mg·dm~(-2) >实生苗( 2.43 ± 0.48) mg·dm~(-2),P_(max)为( 6.77 ± 0.01) μmol·m~(-2)·s~(-1) >实生苗( 5.13 ± 0.002) μmol·m~(-2)·s~(-1).②叶片的生长指标,嫁接苗的叶面积和叶干物质量较高、比叶面积较低,嫁接苗叶面积为( 44.23 ± 5.78) cm~2是实生苗( 24.55 ± 4.57 cm~2 )的1.80倍.③与实生苗相比,嫁接苗的叶片厚度、主脉厚度、表皮厚度、栅栏组织厚度、海绵组织厚度、下表皮气孔密度以及叶脉密度等结构指标均较高.其中,嫁接苗的栅栏组织厚度、主脉厚度及下表皮气孔密度分别高出实生苗35 %、38 %和50 %.【结论】锥栗苗木的光合能力与叶片结构特征关系密切,嫁接苗叶片的生长状况优于实生苗,对光的适应性和光合利用能力强于实生苗.

语种： 中文

作者： 朱周俊;袁德义;邹锋;杨华;范晓明;...

期刊： 食品科学,2019年40(2):165-170 ISSN：1002-6630

作者机构： [邹锋; 朱周俊; 肖诗鑫; 范晓明; 袁德义] 经济林培育与保护教育部重点实验室;湖北省襄阳市保康县公共检验检测中心;中南林业科技大学林学院;[杨华] 保康县公共检验检测中心;[邹昕芸; 李欣; 李果] 中南林业科技大学

关键词： 锥栗;矿质元素;因子分析;聚类分析

摘要： 为探明锥栗种仁中矿质元素含量特征规律,以30种主要锥栗农家种种仁为试材,采用自动间断分析仪与原子吸收光谱法测定N、P、K、Ca、Mg、Fe、Mn、Zn和Cu 9种矿质元素指标,运用相关性分析、因子分析和聚类分析对其矿质元素含量进行分析.结果 表明,Kolmogorov-Smirnov检验表明9种矿质元素含量数据服从正态分布,9种矿质元素平均含量顺序为N (6 873.74 mg/kg) ＞K (4 402.32 mg/kg) ＞P(1 619.16mg/kg)＞Ca (471.18mg/kg) ＞Mg (394.59mg/kg) ＞Mn (115.80mg/kg) ＞Fe (16.22mg/kg) ＞Zn (8.68 mg/kg) ＞Cu(7.59 mg/kg),大量元素中N、K、P与Ca、Mg存在显著差异(P＜0.05),微量元素中Mn与Fe、Zn、Cu差异显著(P＜0.05),变异系数范围为8.29％～54.43％;相关性分析表明各元素之间存在着复杂的关联性;因子分析结果表明,N、P、Mn、K、Cu和Fe是锥栗的特征元素,提取的6个公因子累计方差贡献率为90.572％,第1公因子方差贡献率为22.400％,主要综合了N、P和Mn 3种元素的信息,第2公因子方差贡献率为15.572％,主要综合了K和Cu 2种元素的信息,第3公因子方差贡献率为14.701％,与Fe有关,第4公因子方差贡献率为14.614％,与Mg有关,第5公因子方差贡献率为11.936％,与Ca有关,第6公因子方差贡献率为11.349％,与Zn有关,综合得分排名前5名依次为蔓榛、长芒仔、中尖嘴、材榛和小尖嘴;从元素含量角度进行聚类分析,30种锥栗农家种可分为6类.本研究结果可为进一步开展锥栗营养功能评价、锥栗育种亲本选择和锥栗食品开发等提供基本参考数据.

语种： 中文

作者： Xiong, Huan;Zou, Feng*;Yuan, Deyi*;Tan, Xiaofeng;Yuan, Jun;...

期刊： International Journal of Agriculture and Biology,2019年21(4):819-826 ISSN：1560-8530

通讯作者： Zou, Feng;Yuan, Deyi

作者机构： [Xiong, Huan; Yuan, Jun; Zou, Feng; Yuan, Deyi; Liao, Ting; Tan, Xiaofeng] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;[Niu, Genhua] Texas A&M Univ Syst, Texas AgriLife Res El Paso, 1380 A&M Circle, El Paso, TX 79927 USA.

通讯机构： [Zou, F; Yuan, DY] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.

关键词： Camellia grijsii;Fruit set;Ovarian self-incompatibility;Ovule abortion

摘要： Camellia grijsii Hance is one of the most important woody edible oil tree species in Southern China; however, it often has a low fruit set rate. To elucidate the causes of poor fruit set in C. grijsii, self-pollination (SP) with C. grijsii and cross-pollination (CP) of C. grijsii x C. villosa tests were conducted. Pollen germination and pollen tube growth into pistils, and early ovule development after SP and CP, were examined using a paraffin section and fluorescence microscopy. The fruit set percentage in SP and CP was also investigated. The results showed that pollen germinated normally on the stigma, and the pollen tubes both reached the style base after SP and CP, but the growth rates of pollen differed significantly between SP and CP, being faster for CP. The pollen tubes arrived at the style base 48 h after SP, but only 24 h after CP. No barriers to SP acted at the stigmatic or stylar level in C. grijsii; however, SP pollen tubes stopped at the upside of the ovary at 72 h due to the presence of ovarian self-incompatibility (OSI). There was also no callose deposition in the ovules at 84 h after SP. The inability of SP pollen tubes to penetrate the ovule and the absence of a mature embryo sac in the ovule were the critical factors that led to ovule abortion. Fruit set following SP (2.1%) was consistently and significantly lower than that obtained from CP (72.9%). Thus, we conclude that the presence of strong OSI gives rise to ovule abortion and is the main cause of the poor fruit set in C. grijsii. (C) 2019 Friends Science Publishers

语种： 英文

作者： Xiong, Huan;Zou, Feng*;Guo, Sujuan;Yuan, Deyi;Niu, Genhua

期刊： JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE,2019年144(3):172-181 ISSN：0003-1062

通讯作者： Zou, Feng

作者机构： [Xiong, Huan; Zou, Feng; Yuan, Deyi] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;[Guo, Sujuan] Beijing Forestry Univ, Beijing 100083, Peoples R China.;[Niu, Genhua] Texas A&M Univ Syst, Texas AgriLife Res El Paso, 1380 A&M Circle, El Paso, TX 79927 USA.

通讯机构： [Zou, Feng] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.

关键词： abnormal pollen tubes;double fertilization;ovule aborted;ovary

摘要： Chinese chestnut (Castanea mollissima), which is native to China, has been cultivated as a nontimber forest tree species for 4000 years. This species has been found to display self-sterility, which results in a significantly lower seed set following self-pollination (SP) compared with that following cross-pollination (CP). Self-sterility can be induced by prezygotic or postzygotic late-acting self-incompatibility (LSI) or early-acting inbreeding depression (EID). To elucidate the causes of self-sterility in chestnut, we investigated pollen–pistil interactions, fertilization, and early ovule development following SP and CP by using a paraffin section technique and fluorescence microscopy. Thefruit set percentage and seed characteristics also were evaluated among different pollination treatments. The results show that there were no significant differences in pollen tube behavior following SP vs. CP, regardless of the stigmaticor stylar level. Double fertilization was significantly greater following CP (18.09%) than SP (2.58%). The significantly lower percentages of ovule penetration and double fertilization in the selfed vs. crossed ovules support a prezygotic LSI mechanism in C. mollissima. The fruit set resulting from chase-pollination (CHP; 53.85% to 63.64%) was greater than that resulting from SP (12.12% to 14.00%). In addition, the distribution of aborted seed sizes after SP showed a widely clumped pattern. Abortion occurred at different stages during seed development rather than at a uniform stage, which supported the idea that EID was operating in C. mollissima. Levels of self-sterility in the chinese chestnut trees ranged from 88.2% to 90.5%. Thus, partial prezygotic LSI and EID contributed to self-sterility in the C. mollissima ‘Yanshanzaofeng’, with prezygotic LSI rejecting part of the self-pollen in the ovary and EID aborting part of the self-fertilized seeds. © 2019 Journal of the American Society for Horticultural Science. All rights reserved.

语种： 英文

作者： Li, Ning;Yuan, Deyi;Huang, Li-Jun*

期刊： Transgenic Research,2019年28(5-6):561-572 ISSN：0962-8819

通讯作者： Huang, Li-Jun

作者机构： [Li, Ning; Huang, Li-Jun; Yuan, Deyi] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Huang, Li-Jun] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.

关键词： Agro-infiltration;Binary vector;GAL4-VP16/UAS;Tissue-specific expression;Transgenic plant

摘要： Genetic transformation of plants offers the possibility of functional characterization of individual genes and the improvement of plant traits. Development of novel transformation vectors is essential to improve plant genetic transformation technologies for various applications. Here, we present the development of a Gateway-compatible two-component expression vector system for Agrobacterium-mediated plant transformation. The expression system contains two independent plasmid vector sets, the activator vector and the reporter vector, based on the concept of the GAL4/UAS trans-activation system. The activator vector expresses a modified GAL4 protein (GAL4-VP16) under the control of specific promoter. The GAL4-VP16 protein targets the UAS in the reporter vector and subsequently activates reporter gene expression. Both the activator and reporter vectors contain the Gateway recombination cassette, which can be rapidly and efficiently replaced by any specific promoter and reporter gene of interest, to facilitate gene cloning procedures. The efficiency of the activator-reporter expression system has been assessed using agroinfiltration mediated transient expression assay in Nicotiana benthamiana and stable transgenic expression in Arabidopsis thaliana. The reporter genes were highly expressed with precise tissue-specific and subcellular localization. This Gateway-compatible two-component expression vector system will be a useful tool for advancing plant gene engineering.

语种： 英文

作者： 高超;杨瑞;郭其强;袁德义

期刊： 林业科学研究,2019年32(1):1-7 ISSN：1001-1498

通讯作者： Yuan, D.-Y.

作者机构： [郭其强] Institute for Forest Resources & Environment of Guizhou, Guizhou University, Guiyang, Guizhou 550025, China;[杨瑞] College of Forestry, Guizhou University, Guiyang, Guizhou 550025, China;[袁德义] Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education and the Key Laboratory of Non-Wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha, Hunan 410004, China;[高超] Institute for Forest Resources & Environment of Guizhou, Guizhou University, Guiyang, Guizhou 550025, China, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education and the Key Laboratory of Non-Wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha, Hunan 410004, China

通讯机构： [Yuan, D.-Y.] K;Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education and the Key Laboratory of Non-Wood Forest Products of State Forestry Administration, China

关键词： 油茶;柱头;花柱;显微结构;超微结构

摘要： [目的]从显微和超微层面探究油茶柱头和花柱的结构特征,可为探讨油茶自交不亲和性的细胞学机制提供理论依据,同时也为油茶杂交育种工作奠定基础。[方法]以开花当天的雌蕊为试验材料,利用细胞学方法研究油茶柱头和花柱的显微及超微结构特征。[结果]油茶雌蕊具完整的柱头、花柱和子房结构,湿性柱头其表面密布乳突细胞,排列紧密,细胞质浓厚,细胞器丰富,细胞间具有丰富的分泌物。基本组织细胞内含物和细胞器较少,细胞壁薄且壁外无分泌物。整个花柱由3 5根独立花柱组成,上端约2 /3部分相互分离形成离生区,从2 /3处开始到花柱基部相互连接形成合生区。每根花柱中空,由外表皮、基本组织和花柱道内表皮构成。花柱通道细胞外充满大量分泌物,该细胞比基本组织细胞大而规则、细胞质浓厚,具有腺质细胞的特点。花柱道从上到下贴合逐渐紧密,花柱基部的各花柱道之间相互连通,形成狭小的缝隙。[结论]本研究分析了油茶柱头和花柱的显微及超微结构特征,探明柱头乳突和花柱通道细胞为分泌型细胞,基本组织细胞结构单一,从柱头到子房花柱道逐渐变窄,在基部相互连通形成狭小的缝隙,花柱通道细胞从上至下壁外分泌物和细胞内细胞质浓度、细胞器数量与种类具有逐渐增加的趋势和特点。

语种： 中文

作者： 沈植国;岳长平;孙萌;袁德义;程建明

期刊： 园艺学报,2019年(S02):2904-2905 ISSN：0513-353X

作者机构： 1. 中南林业科技大学林学院;2. 河南省林业科学研究院;3. 鄢陵县林业科学研究所河

关键词： 蜡梅;实生选育;品种

摘要： ‘豫素1号’蜡梅是实生选育出的优良新品种。花碗形,花径中等,花被片深黄,香味浓郁,以短枝成花为主。耐旱耐瘠薄,抗寒耐修剪,既能用于城市园林绿化,也可用于家庭园艺插花。

语种： 中文

作者： Gao, Chao;Yang, Rui;Yuan, Deyi*

期刊： Nordic Journal of Botany,2018年36(6):njb-01673- ISSN：0107-055X

通讯作者： Yuan, Deyi

作者机构： [Gao, Chao] Guizhou Univ, Inst Forest Resources & Environm Guizhou, Guiyang, Guizhou, Peoples R China.;[Yang, Rui] Guizhou Univ, Coll Forestry, Guiyang, Guizhou, Peoples R China.;[Yuan, Deyi] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Hunan, Peoples R China.;[Yuan, Deyi] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod, Forestry Minist, Changsha, Hunan, Peoples R China.

通讯机构： [Yuan, Deyi] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod, Forestry Minist, Changsha, Hunan, Peoples R China.

关键词： ovule;embryo sac;female germ unit;three-dimensional structure;ultrastructure;confocal laser scanning microscope

摘要： The ovule is the most important reproductive organ in the pistil of phanerogamae. Camellia oleifera (Theaceae) is an important woody plant producing edible oil in southern China, and its embryo sac structure has a positive effect on seed breeding. In this study, the microstructure, ultrastructure and three-dimensional structure of the ovule and embryo sac of C. oleifera were observed and described based on a combination of advanced microscopy techniques (SEM, TEM, CLSM). The ovule comprises the inner and outer integument. Large quantities of secretions in the micropylar canal exit and may participate in the guidance of the entry of the pollen tube into the embryo sac. The synergids have a dense cytoplasm, abundant organelles, and strong polarity. Little cytoplasm is present in the egg cell, yet there are many vacuoles. The center of the cell is taken up by a large vacuole, and the cytoplasm is pushed towards the edges to form obvious cytoplasmic cords. The two polar nuclei are large and conspicuous. The antipodal cells degenerate to fulfill a nutritional function. © 2018 The Authors

语种： 英文

作者： Peng, Dan;Tan, Xiaofeng;Zhang, Lin;Yuan, Deyi;Lin, Jianzhong;...

期刊： Breeding Science,2018年68(3):326-335 ISSN：1344-7610

通讯作者： Zhou, Bo

作者机构： [Zhou, Bo; Jiang, Yueqiao; Peng, Dan] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Tree, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Cent Cultivat & Utilizat Nonwo, Changsha 410004, Hunan, Peoples R China.;[Zhou, Bo; Liu, Xuanming; Lin, Jianzhong] Hunan Univ, Key Lab Plant Funct Genom Dev & Regulat, Changsha 410082, Hunan, Peoples R China.

通讯机构： [Zhou, Bo] C;[Zhou, Bo] H;[Zhou, Bo] F;Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;Hunan Univ, Key Lab Plant Funct Genom Dev & Regulat, Changsha 410082, Hunan, Peoples R China.

关键词： branching;seed yield;OsPAT15;B. napus L.;sulfur acyl transferase

摘要： Branching is a predominant element in the plant architecture of Brassica napus L. and represents an important determinant of seed yield. OsPAT15 (OsDHHC1), a novel DHHC-type zinc finger protein gene, was reported to regulate rice plant architecture by altering the tillering. However, whether heterologous overexpression of the OsPAT15 gene from the monocot rice into the dicot B. napus L. would have the same effect on branching or seed yield is unknown. In this study, the DHHC-type zinc finger protein gene OsPAT15 was determined to have sulfur acyl transferase activity in the akr1Δ yeast mutant in a complementation experiment. Heterologously expressing OsPAT15 transgenic B. napus L. plants were obtained using the Agrobacterium-mediated floral-dip transformation method. As anticipated, OsPAT15 transgenic plants exhibited branching and seed yield. Compared with non-transgenic plants, OsPAT15 transgenic plants had increased primary branches (1.58–1.76-fold) and siliques (1.86–1.89-fold), resulting in a significant increase in seed yield (around 2.39– 2.51-fold). Therefore, overexpression of the sulfur acyl transferase gene OsPAT15 in B. napus L. could be used to increase seed yield and produce excellent varieties. © 2018, Japanese Society of Breeding. All rights reserved.

语种： 英文

作者： Peng, Dan;Zhou, Bo*;Jiang, Yueqiao;Tan, XiaoFeng;Yuan, DeYi;...

期刊： Plant Science,2018年272:32-41 ISSN：0168-9452

通讯作者： Zhou, Bo

作者机构： [Zhou, Bo; Jiang, Yueqiao; Peng, Dan] Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Cent Cultivat & Utilizat Nonwo, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Peng, Dan] Forestry Biotechnol Hunan Key Labs, Changsha 410018, Hunan, Peoples R China.

通讯机构： [Zhou, Bo] C;Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410018, Hunan, Peoples R China.

关键词： Freezing tolerance;Sapium sebiferum (L.) Roxb.;Stearoyl-acyl carrier protein desaturase;Unsaturated fatty acid

摘要： Sapium sebiferum (L.) Roxb. is an important woody oil tree and traditional herbal medicine in China. Stearoyl-acyl carrier protein desaturase (SAD) is a dehydrogenase enzyme that plays a key role in the transformation of saturated fatty acids into unsaturated fatty acids in oil; these fatty acids greatly influence the freezing tolerance of plants. However, it remains unclear whether freezing tolerance can be regulated by the expression level of SsSAD in S. sebiferum L. Our research indicated that SsSAD expression in S. sebiferum L. increased under freezing stress. To further confirm this result, we constructed a pEGAD-SsSAD vector and transformed it into B. napus L. W10 by Agrobacterium tumefaciens-mediated transformation. Transgenic plants that overexpressed the SsSAD gene exhibited significantly higher linoleic (18:2) and linolenic acid (18:3) content and advanced freezing tolerance. These results suggest that SsSAD overexpression in B. napus L. can increase the content of polyunsaturated fatty acids (PUFAs) such as linoleic (18:2) and linolenic acid (18:3), which are likely pivotal in improving freezing tolerance in B. napus L. plants. Thus, SsSAD overexpression could be useful in the production of freeze-tolerant varieties of B. napus L. © 2018 Elsevier B.V.

语种： 英文