摘要：
The INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) controls floral organ abscission in plants. IDA belongs to IDA-LIKE (IDL) gene family that is involved in regulation of Arabidopsis development. Herein, we identified three genes, CoIDA1, CoIDA2 and CoIDA3 in Camellia oleifera (Camellia oleifera Abel. cv. Huashuo) and suggested their involvement in the regulation of fruits abscission. The full-length cDNA sequences of CoIDA1, CoIDA2 and CoIDA3 were of 207 bp, 276 bp and 273 bp, encoding proteins of 68, 91 and 90 amino acids, respectively. These CoIDA genes were single exon genes (SEGs) with a conserved extended PIP motif (EPIP) at C-terminal that has been implicated to play an important role in governing protein function for enhanced flower abortion rate. The highest expression of CoIDA1 was in young peduncles and the lowest in young fruits. However, the highest expressions of CoIDA2 and CoIDA3 were both in young roots, and the lowest in young fruits. The expressions of CoIDA1 and CoIDA2 significantly increased in abscission zones (AZs) of both abnormal fruits (AF) and ethephon treated fruits (ETH-F) with respect to normal fruits (NF), which suggest that CoIDA1 and CoIDA2 genes are related to fruits abscission in C. oleifera. This study provided a preliminary understanding about CoIDA genes which could lead to their detailed functional analysis and utilization for improving C. oleifera yield potential.
作者机构：
[袁德义] College of Forestry, Central South University of Forestry and Technology, Changsha, 410004, China;[尚忠海; 丁鑫; 孙萌] Henan Academy of Forestry, Zhengzhou, 450008, China;[程建明] Henan Kalefu Horticulture Limited Company, Zhengzhou, 450000, China;[沈植国] College of Forestry, Central South University of Forestry and Technology, Changsha, 410004, China, Henan Academy of Forestry, Zhengzhou, 450008, China
通讯机构：
[SHANG, Z.] H;Henan Academy of ForestryChina
摘要：
Camellia oleifera Abel., belonging to the genus Camellia of Theaceae, has been widely used as a cooking oil, lubricant, and in cosmetics. Because of complicated polyploidization and large genomes, reference genome information is still lacking. Systematic characterization of gene models based on transcriptome data is a fast and economical approach for C. oleifera. Pacific Biosciences single-molecule long-read isoform sequencing (Iso-Seq) and Illumina RNA-Seq combined with gas chromatography were performed for exploration of oil biosynthesis, accumulation, and comprehensive transcriptome analysis in C. oleifera seeds at five different developmental stages. We report the first full-length transcriptome data set of C. oleifera seeds comprising 40,143 deredundant high-quality isoforms. Among these isoforms, 37,982 were functionally annotated, and 271 (2.43%) belonged to fatty acid metabolism. A total of 8,344 full-length unique transcript models were obtained, and 8,151 (97.69%) of them produced more than two isoforms, suggesting a high degree of transcriptome complexity in C. oleifera seeds. A total of 783 alternative splicing (AS) events were identified, among which the retained intron was the most abundant. We also obtained 1,910 long noncoding RNAs (lncRNAs) and found that AS events occurred in these lncRNAs. Potential transcript variants of genes involved in oil biosynthesis were also investigated. After performing weighted correlation network analysis, we found seven "gene modules" and hub genes for each module showing a significant association with oil content. The series test of clusters classified these modules into four significant profiles based on gene expression patterns. Protein-protein interaction network analysis showed that upregulated WRI1 interacted with 17 genes encoding the enzymes playing key roles in oil synthesis. MYB and ZIP transcriptional factors also showed significant interactions with key genes involved in oil synthesis. Collectively, our data advance the knowledge of RNA isoform diversity in seeds at different developmental stages and provide a rich resource for functional studies on oil synthesis in C. oleifera.
作者机构：
[李华威; 袁德义] College of Forestry, Central South University of Forestry and Technology, Changsha, 410004, China;[王丽媛; 刁松锋; 韩卫娟; 买旖旎; 索玉静; 傅建敏; 孙鹏] Non-Timber Forest Research and Development Center, China Paulownia Research Center, State Forestry Administration, Chinese Academy of Forestry, Zhengzhou, 450003, China
摘要：
Genetic transformation of plants offers the possibility of functional characterization of individual genes and the improvement of plant traits. Development of novel transformation vectors is essential to improve plant genetic transformation technologies for various applications. Here, we present the development of a Gateway-compatible two-component expression vector system for Agrobacterium-mediated plant transformation. The expression system contains two independent plasmid vector sets, the activator vector and the reporter vector, based on the concept of the GAL4/UAS trans-activation system. The activator vector expresses a modified GAL4 protein (GAL4-VP16) under the control of specific promoter. The GAL4-VP16 protein targets the UAS in the reporter vector and subsequently activates reporter gene expression. Both the activator and reporter vectors contain the Gateway recombination cassette, which can be rapidly and efficiently replaced by any specific promoter and reporter gene of interest, to facilitate gene cloning procedures. The efficiency of the activator-reporter expression system has been assessed using agroinfiltration mediated transient expression assay in Nicotiana benthamiana and stable transgenic expression in Arabidopsis thaliana. The reporter genes were highly expressed with precise tissue-specific and subcellular localization. This Gateway-compatible two-component expression vector system will be a useful tool for advancing plant gene engineering.
作者机构：
[郭其强] Institute for Forest Resources & Environment of Guizhou, Guizhou University, Guiyang, Guizhou 550025, China;[杨瑞] College of Forestry, Guizhou University, Guiyang, Guizhou 550025, China;[袁德义] Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education and the Key Laboratory of Non-Wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha, Hunan 410004, China;[高超] Institute for Forest Resources & Environment of Guizhou, Guizhou University, Guiyang, Guizhou 550025, China, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education and the Key Laboratory of Non-Wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
通讯机构：
[Yuan, D.-Y.] K;Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education and the Key Laboratory of Non-Wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha, Hunan, China
摘要：
The ovule is the most important reproductive organ in the pistil of phanerogamae. Camellia oleifera (Theaceae) is an important woody plant producing edible oil in southern China, and its embryo sac structure has a positive effect on seed breeding. In this study, the microstructure, ultrastructure and three-dimensional structure of the ovule and embryo sac of C. oleifera were observed and described based on a combination of advanced microscopy techniques (SEM, TEM, CLSM). The ovule comprises the inner and outer integument. Large quantities of secretions in the micropylar canal exit and may participate in the guidance of the entry of the pollen tube into the embryo sac. The synergids have a dense cytoplasm, abundant organelles, and strong polarity. Little cytoplasm is present in the egg cell, yet there are many vacuoles. The center of the cell is taken up by a large vacuole, and the cytoplasm is pushed towards the edges to form obvious cytoplasmic cords. The two polar nuclei are large and conspicuous. The antipodal cells degenerate to fulfill a nutritional function.
通讯机构：
Key Laboratory of Cultivation and Protection for Non-wood Forest Trees of Ministry of Education, Central South University of Forestry and Technology, Changsha, China
通讯机构：
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education, The Key Laboratory of Non-Wood Forest Products of Forestry Ministry, Central South University of Forestry and Technology, Changsha, China
期刊：
The Open Biotechnology Journal,2017年11(1):9 -15 ISSN：1874-0707
通讯作者：
Yuan, D.
作者机构：
The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan 410004, China;Chinese Academy of Forestry, Beijing, 100091, China;Texas A&M AgriLife Research Center at El Paso, Texas A&M University System, 1380 A&M Circle, El Paso, TX 79927, United States
通讯机构：
The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan, China
