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Development of a Gateway-compatible two-component expression vector system for plants.

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成果类型:
期刊论文
作者:
Li, Ning;Yuan, Deyi;Huang, Li-Jun*
通讯作者:
Huang, Li-Jun
作者机构:
[Li, Ning; Huang, Li-Jun; Yuan, Deyi] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.
通讯机构:
[Huang, Li-Jun] C
Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.
语种:
英文
关键词:
Agro-infiltration;Binary vector;GAL4-VP16/UAS;Tissue-specific expression;Transgenic plant
期刊:
Transgenic Research
ISSN:
0962-8819
年:
2019
卷:
28
期:
5-6
页码:
561-572
基金类别:
Special Fund for Forestry Scientific Research in the Public Interest [201504705]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31901345]
机构署名:
本校为第一且通讯机构
院系归属:
林学院
摘要:
Genetic transformation of plants offers the possibility of functional characterization of individual genes and the improvement of plant traits. Development of novel transformation vectors is essential to improve plant genetic transformation technologies for various applications. Here, we present the development of a Gateway-compatible two-component expression vector system for Agrobacterium-mediated plant transformation. The expression system contains two independent plasmid vector sets, the activator vector and the reporter vector, based on the concept of the GAL4/UAS trans-activation system....

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