期刊论文

Cloning and expression analysis of Camellia oleifera transporter gene ABCB26

Tan, X.-F.

中文

植物生理与分子生物学学报

1671-3877

2019

55

11

1664-1672

10.13592/j.cnki.ppj.2019.0332

国家自然科学基金(31730016)~~

本校为第一且通讯机构

以油茶(Camellia oleifera) ‘华硕'品种为研究对象,通过RT-PCR克隆得到腺苷三磷酸结合盒转运蛋白ABCB基因家族的一个成员,命名为CoABCB26。该基因的开放阅读框(ORF)为1 491 bp,共编码496个氨基酸。序列比对发现,油茶CoABCB26基因与茶树(Camellia sinensis)的CsABCB26基因亲缘关系最近,相似性达99%,且具有ABC转运蛋白3个特殊结构域: Walker A结构域、ABC标签序列和Walker B结构域。CoABCB26转运蛋白为稳定的疏水性蛋白,相对分子质量为55.88 kDa,理论等电点为8.69,具有2个跨膜结构域,但不具有信号肽;其二级结构主要由α螺旋和无规卷曲两种元件构成。实时定量PCR结果表明, CoABCB26基因在芽和花粉...

A member of ATP binding cassette transporter named CoABCB26 which belonged to ABCB gene family was cloned by RT-PCR from Camellia oleifera cultivar 'Huashuo'. The ORF of the gene was 1 491 bp and encoded 496 amino acids. Sequence alignment revealed that CoABCB26 gene of Camellia oleifera had the nerest genetic relationship with CsABCB26 gene of Camellia sinensis and shared 99% homology with it. There were three characteristic domains of ABC transporter: Walker A domain, ABC signature sequence and Walker B domain. CoABCB26 transporter was a stab...