The Method of Screening and Identification of Transcription Factor in Klebsiella.

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成果类型：

期刊论文

作者：

Wu, Qiang;Liu, Gao-Qiang;Ma, Jiang-Shan;Li, Qiang

作者机构：

[Wu, Qiang] College of Food and Chemical Engineering, Shaoyang University, Shaoyang, China. qiangwusmile@163.com

[Li, Qiang; Ma, Jiang-Shan; Liu, Gao-Qiang; Wu, Qiang] Hunan Provincial Key Laboratory of Forestry Biotechnology &

[Wu, Qiang] International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha, China. qiangwusmile@163.com

[Wu, Qiang] Microbial Variety Creation Center, National Laboratory of Yuelushan Seed Industry, Changsha, China. qiangwusmile@163.com

[Li, Qiang; Ma, Jiang-Shan; Liu, Gao-Qiang] International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha, China

语种：

英文

关键词：

DNA pull-down;EMSA;Gene knockout;Transcription factor

期刊：

Methods in Molecular Biology

ISSN：

1064-3745

年：

2023

卷：

2594

页码：

13-28

DOI：

10.1007/978-1-0716-2815-7_2

机构署名：

本校为其他机构

院系归属：

生命科学与技术学院

摘要：

This protocol describes a method for verifying the specific transcription factor regulating glycerol dehydratase (GDH) expression in Klebsiella. DNA pull-down accompanied with mass spectrometry is used to screen and identify the transcription factor interacting with the promoter region of the key gene in Klebsiella. EMSA method is used to validate the specific binding of the transcription factor to the promoter region in vitro. In addition, the target DNA fragments are constructed by fusion PCR to prepare competent cells from Klebsiella for electrical transformation and further transformed to ...

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The Method of Screening and Identification of Transcription Factor in Klebsiella.

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