摘要：
本发明公开一种脂肪酸去饱和酶基因及其在检测和培育高亚麻酸油茶的应用。所述基因的CDS序列如SEQ ID No：3-5任一所示。其表达水平与油茶种仁亚麻酸含量的高低呈正相关关系。本发明中两种等位变异在CoFAD7的CDS序列830位置对应的碱基分别是G和G/A。首先以油茶cDNA为模板,利用引物对SEQ ID No：8和SEQ ID No：9进行PCR扩增,经Sanger测序检测,可方便有效地实现CoFAD7基因不同等位变异在油茶种质资源以及育种后代中的鉴定。本发明通过常规的PCR和Sanger测序就可以完成,操作方便,且稳定可行。
期刊：
Journal of Environmental Management,2024年351:119688 ISSN：0301-4797
通讯作者：
Zhang, C;Yan, WD
作者机构：
[Ye, Zhencheng; Zhang, Chao; Wang, Wancai; Zhang, C; Li, Jing] Northwest A&F Univ, Key Lab Soil Eros & Dryland Farming Loess Plateau, Yangling 712100, Peoples R China.;[Liu, Guobin; Zhang, Chao] Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.;[Wang, Zhaoyang; Wu, Qixiao; He, Guoqin] Cent South Univ Forestry & Technol, Bangor Coll China Joint Unit Bangor Univ, Changsha 410004, Hunan, Peoples R China.;[Yan, Wende] Cent South Univ Forestry & Technol, Natl Engn Lab Appl Technol Forestry & Ecol South C, Changsha 410004, Peoples R China.
通讯机构：
[Zhang, C ] N;[Yan, WD ] C;Northwest A&F Univ, Key Lab Soil Eros & Dryland Farming Loess Plateau, Yangling 712100, Peoples R China.;Cent South Univ Forestry & Technol, Natl Engn Lab Appl Technol Forestry & Ecol South C, Changsha 410004, Peoples R China.
关键词：
Abundant taxa;Agricultural ecosystem;Greenhouse gas emissions;Multinutrient cycling;Rare taxa
摘要：
The field practices, including irrigation and fertilization, strongly affect greenhouse gas emissions and soil nutrient cycling from agriculture. Understanding the underlying mechanism of greenhouse gas emissions, soil nutrient cycling, and their impact factors (fungal diversity, network characteristics, soil pH, salt, and moisture) is essential for efficiently managing global greenhouse gas mitigation and agricultural production. By considering abundant and rare taxa, we determine the identities and relative importance of ecological processes that modulate the fungal communities and identify whether they are crucial contributors to soil nutrient cycling and greenhouse gas emissions. The research is based on a 4-year field fertigation experiment with low (300kg/ha P(2)O(5) with 150kg/ha urea) and high (600kg/ha P(2)O(5) with 300kg/ha urea) fertilization level and three irrigation levels, that is, low (200mm), medium (300mm), and high (400mm). The α-diversity (richness and Shannon index) of fungal subcommunities was significantly higher under medium irrigation (300mm) and low fertilization (300kg/ha P(2)O(5) with 150kg/ha urea) than under other treatments. Intermediate irrigation with low fertilization treatment yielded the most significant higher multinutrient cycling index and the lowest CO(2) and CH(4) emissions. The null model indicated that abundant taxa are mainly regulated by stochastic processes (dispersal limitation), and rare taxa are mainly regulated by environmental selection, especially by soil salinity. The co-occurrence network of rare taxa explained the changes in the entire fungal network stability. The abundant taxa played vital roles in regulating soil nutrient status, owing to the stronger association between their network and multinutrient cycling index. Furthermore, we have confirmed that soil moisture and fungal network stability are crucial factors affecting greenhouse gas emissions. Together, these results provide a deep understanding of the mechanisms that reveal fungal community assembly and soil fungal-driven variations in nutrient status and network stability, link fungal network characteristics to ecosystem functions, and reveal the factors that influence greenhouse gas emissions.
作者机构：
[Li, He; Zhu, Yuanye; Ma, Mengting] College of Forestry, Central South University of Forestry and Technology, Changsha, China;[Li, He; Zhu, Yuanye; Ma, Mengting] Key Laboratory of National Forestry, Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Changsha, China;[Li, He; Zhu, Yuanye; Ma, Mengting] Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Changsha, China;[Li, He; Zhu, Yuanye; Ma, Mengting] Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Changsha, China
关键词：
Colletotrichum fructicola;carbendazim;mechanism of resistance;β-tubulin isotypes
摘要：
Colletotrichum fructicola is the major pathogen of anthracnose in tea-oil trees in China. Control of anthracnose in tea-oil trees mainly depends on the application of chemical fungicides such as carbendazim. However, the current sensitivity of C. fructicola isolates in tea-oil trees to carbendazim has not been reported. Here, we tested the sensitivity of 121 C. fructicola isolates collected from Guangdong, Guangxi, Guizhou, Hainan, Hunan, Jiangsu, and Jiangxi provinces in China to carbendazim. One hundred and ten isolates were sensitive to carbendazim, and 11 isolates were highly resistant to carbendazim. The growth rates, morphology, and pathogenicity of three resistant isolates were identical to those of three sensitive isolates, which indicates that these resistant isolates could form a resistant population under carbendazim application. These results suggest that carbendazim should not be the sole fungicide in control of anthracnose in tea-oil trees; other fungicides with different mechanisms of action or mixtures of fungicides could be considered. In addition, bioinformatics analysis identified two β-tubulin isotypes in C. fructicola: Cfβ(1)tub and Cfβ(2)tub. E198A mutation was discovered in the Cfβ(2)tub of three carbendazim-resistant isolates. We also investigated the functional roles of two β-tubulin isotypes. CfΔβ(1)tub exhibited slightly increased sensitivity to carbendazim and normal phenotypes. Surprisingly, CfΔβ(2)tub was highly resistant to carbendazim and showed a seriously decreased growth rate, conidial production, pathogenicity, and abnormal hyphae morphology. Promoter replacement mutant CfΔβ(2)-2×β(1) showed partly restored phenotypes, but it was still highly resistant to carbendazim, which suggests that Cfβ(1)tub and Cfβ(2)tub are functionally interchangeable to a certain degree.
作者机构：
[Liu, Falin; Liu, FL; Chen, Hao] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.
通讯机构：
[Liu, FL ] C;Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.
关键词：
Fire disturbance;Ecosystem recovery;Subtropical climate;Prescribed burning
摘要：
Soil water repellency (SWR) is a physical phenomenon whereby the surface of soil particles cannot be, or resists being, moistened by water. The recovery process of fire-induced SWR can provide important evidence for ecosystem resilience, but most existing studies ignored this process. Prescribed burning is a widely used method to reduce the risk of fire and fuel. However, prescribed fires can increase SWR, reducing infiltration and increasing overland flow and subsequent soil erosion. In order to understand the ecosystem resilience process from fire-induced SWR, soil samples were collected before and after prescribed burning. Four different forest types in the Hunan province of China were studied. Soil samples at four different soil depths (0-5, 6-10, 11-15, and >15 cm) and four time intervals (3, 30, 180, and 360 d) after burning at different intensities were collected. No significant difference in SWR was found among the four forest types, but the SWR of soil in plantations was stronger than that in secondary forests before fire. Most soil samples showed slightly increased SWR after burning, SWR increasing with fire intensity. Surface soil (0-5 cm) SWR showed greater sensitivity to fire disturbance than subsurface soil (>5 cm). SWR had a smaller scope of influence and shorter recovery period in burned secondary forests. Although SWR recovered over time, recovery was slower with increasing fire intensity. SWR always recovered to pre-fire levels given sufficient time. Our study revealed the recovery process of SWR, and explored the temporal and spatial mechanisms of forest ecosystem recovery after prescribed burning.
期刊：
Journal of Luminescence,2024年268:120434 ISSN：0022-2313
通讯作者：
Zhang, XM
作者机构：
[Zhang, XM; Long, Fenzhong; Wu, Pianpian; Fu, Jiawei; Zhang, Xinmin] Cent South Univ Forestry & Technol, Sch Mat Sci & Engn, Hunan Prov Key Lab Mat Surface & Interface Sci & T, Changsha 410004, Peoples R China.
通讯机构：
[Zhang, XM ] C;Cent South Univ Forestry & Technol, Sch Mat Sci & Engn, Hunan Prov Key Lab Mat Surface & Interface Sci & T, Changsha 410004, Peoples R China.
关键词：
Chloroborate;Phosphor;Site occupation;White LED
摘要：
Investigation on Ce3+ ions occupying different cation sites of a specific compound is of great significance for exploring multicolor phosphors. Herein, we report the luminescent properties of Ce3+ in chloroborate Ca2BO3Cl host. Two kinds of Ce3+ f -d transitions have been observed via adjusting the doping concentration. The assignment for different Ce3+ sites to certain cation sites is corroborated by structural analysis (XRD), fluorescence spectroscopy (PL) and decay kinetic analysis. Information on the two Ce3+ sites' wavelengths of the five 5d bands in the Ca2BO3Cl host lattice together with ecfs, ec, D and Delta S are obtained. Moreover, with the increasing of Ce3+ doping concentration, the emission peak shows an obviously redshift, and the emission color changes from dark blue to blue. The tuning luminescence properties are attributed to the energy transfer from Ce II to Ce I. The optical properties of the as-fabricated white light-emitting diode (LED) are studied. However, the optical properties of current studied Ce3+ doped Ca2BO3Cl is not good enough to be a candidate for near-UV chip excited phosphor-converted white LED.
作者：
Long Liang;Ting Wu;Kuizhong Shen;Guigan Fang;Yiqiang Wu;...
期刊：
Cellulose,2024年:1-14 ISSN：0969-0239
通讯作者：
Kuizhong Shen<&wdkj&>Guigan Fang
作者机构：
[Long Liang; Ting Wu; Kuizhong Shen; Guigan Fang; Shanming Han; Mengke Zhao] Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Key Lab. of Biomass Energy and Material, Jiangsu Province;Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration, National Engineering Lab. for Biomass Chemical Utilization, Nanjing, China;State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China;Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, China;[Yiqiang Wu] School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, China
通讯机构：
[Kuizhong Shen; Guigan Fang] I;Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Key Lab. of Biomass Energy and Material, Jiangsu Province;Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration, National Engineering Lab. for Biomass Chemical Utilization, Nanjing, China<&wdkj&>Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, China<&wdkj&>Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Key Lab. of Biomass Energy and Material, Jiangsu Province;Key Lab. of Chemical Engineering of Forest Products, National Forestry and Grassland Administration, National Engineering Lab. for Biomass Chemical Utilization, Nanjing, China<&wdkj&>Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, China
摘要：
In chemi-mechanical pulping process, selective fiber separation strategies directly affect pulp property. The secondary wall (SW) separation strategy and middle lamella (ML) separation strategy expose more chemical structures from polysaccharides and lignin on the pulp fiber surface, respectively. Hydrogen-containing groups in these chemical structures generate characteristic spectral changes in response to water perturbation, which makes it possible for surface property analysis of pulp fibers based on near-infrared spectroscopy (NIR). Here, two-dimensional correlation spectroscopy (2DCOS) was obtained by the dynamic NIR spectra of pulp fibers with various equilibrium moisture content (EMC). The 2DCOS-NIR can provide richer structural and distributional information about hydrogen-containing groups which characterize the differences in surface chemical properties due to selective separation of the fibers. In synchronous map, the fibers separated at SW have significant water perturbation-induced spectral changes at the bands due to polysaccharides, and the fibers separated at ML have characteristic spectral changes at the bands due to hydrogen-containing groups from lignin. Furthermore, the 2DCOS spectral features can also accurately reflect the variation in the distribution of hydrogen bonding on the fiber surface under different refining conditions, which directly affects the strength properties of pulp fibers. The PLSR model based on 2DCOS spectral features exhibits excellent and robust predictive performance for internal bond strength with RMSEp of 7.17 J/m2, R of 0.9378 and RPD value of 4.11.
摘要：
Transformation of biomass wastes or residues into functional carbon materials is one of the main approaches to improve their added value and utilization rate. In this work, an example of discarded bamboo fibers was used to fabricate trimetallic functionalized 1D carbon composite with excellent electromagnetic wave (EW) absorption performances, via a simple solvothermal-carbonization method. When the thickness is below 2.0 mm, the composite has a minimum reflection loss (RLmin) of -61.6 dB and a specific RL value of -253 dB mg-1 mm-1, as well as an effective absorption band of 5.4 GHz at a low mass loading of 15 wt%. Such good performances are attributed to the synergetic effect of dielectric loss from bamboo fiber carbon and magnetic loss from Fe-Co-Ni interaction that greatly improved the impedance matching. In addition, bamboo fibers with rough surface could endow ample core-shell FeCoNi nanoparticles to uniform embed, which brings large number of interfaces and defects for the composite to enhance the electromagnetic loss capacity. This work provides a trimetallic compositing idea and a sustainable design approach for using biomass wastes to produce stable and efficient EW absorption materials.
