Chloroplasts, essential to the photosynthetic process, are pivotal in orchestrating the development and growth of plants. The pyruvate dehydrogenase complex (PDC) serves as a link between the citric acid cycle and glycolysis, supplying energy and carbon skeletons vital for plant metabolism. However, the involvement of the PDC in chloroplast development is not yet fully elucidated. Here, we provide evidence for the role of the PDC E1 component β subunit (PDC-E1β) in tobacco ( Nicotiana tabacum L.) chloroplast development and amino acid metabol...