作者机构：
[林森; 李科林] School of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha;410004, China;[肖谋良; 魏亮; 刘守龙; 祝贞科; 葛体达; 吴金水] Key Laboratory of Subtropical Agro-Ecological Processes, Institute of Subtropical Agro-Ecology, Chinese Academy of Sciences, Changsha;410125, China;[江家彬] College of Agriculture, Guizhou University, Guiyang
作者机构：
[柏菁; 李奕霏; 李科林; 彭佩钦] College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha;410004, China;[刘守龙; 祝贞科; 葛体达; 吴金水] Key Laboratory of Agro-Ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha;410125, China;[吴小红] College of Life Science and Technology, Central South University of Forestry and Technology, Changsha
摘要：
Verticillium insectorum J3 was isolated from a local lead-zinc deposit tailing, and its biosorption characteristics and reaction to the toxicities of different Pb(II) and Zn(II) concentrations were investigated. SEM, FTIR, a pH test and a desorption experiment were carried out to identify a possible mechanism. The biosorption of J3 presented an inhibition trend at low concentrations (25-75 mg L-1) and promotion at high concentrations (100-300 mg L-1). J3 absorbed Pb(II) prior to Zn(II) and produced alkaline substances, while mycelial and pellet morphology modifications were important for the removal of Pb(II) and Zn(II) under different stressful conditions (SEM results). Both intracellular accumulation and extracellular absorption may contribute to the removal of Pb(II) at lower concentrations (25-50 mg L-1), although mainly extracellular biosorption occurred at higher concentrations (75-300 mg L-1). However, Zn(II) bioaccumulation occurred at all concentrations assayed. Verticillium insectorum J3 may have evolved active defenses to alleviate the toxicity of heavy metals and proved to be a highly efficient biosorbent, especially for Pb(II) at high concentrations. This study is a useful reference for the development of biotreatment technologies to mitigate heavy metal waste.
摘要：
In this study, Salix matsudana activated carbon (SAC) was prepared by phosphoric acid activation, and the adsorption characteristics of Cd(II) and Pb(II) on SAC in single-and double-component solutions were investigated. In both systems, the adsorption capacities of both ions on SAC increased with the increasing initial pH value and temperature in the solutions, and the adsorption equilibrium was approached at 10 min. The adsorption process was spontaneous, endothermic, and depicted well by the pseudo-second-order adsorption model, and the equilibrium adsorption fitted reasonably well with the Langmuir isotherm. The maximum adsorption capacity (Q(m)) of Cd(II) and Pb (II) was 58.48 and 59.01 mg/g, respectively, in the single-element systems. However, it reduced to 25.32 and 31.09 mg/g, respectively, in the double-element system. The physicochemical property analysis showed that the specific surface area, total pore volume, and average pore diameter of SAC was 435.65 m(2)/g, 35.68 mL/g, and 3.86 nm, respectively. The SAC contained groups of -OH, C=O, and P=O. Results suggest that SAC had a good performance for the adsorption of Cd(II) and Pb(II) from solution, and the adsorption selectivity sequence was Pb(II) > Cd(II).
作者机构：
[舒艳; 李芸; 李科林; 汤春芳] College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, China
通讯机构：
[Tang, C.] C;College of Environmental Science and Engineering, China
作者机构：
[宋金风; 李科林; 汤春芳; 舒艳; 李冰] College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, China
通讯机构：
[Tang, C.] C;College of Environmental Science and Engineering, China
摘要：
【目的】针对湖南资兴铅锌矿污染问题,筛选本土耐性菌株用作生物修复。【方法】供试菌株J3筛选自湖南资兴铅锌矿区的尾砂矿矿渣,利用正交实验分析其生长菌株及干菌体最佳吸附条件以优化其吸附效果,同时对相关数据进行动力学拟合以探讨其吸附机理,最后结合形态观察和18S r RNA基因序列分析对其进行分类鉴定。【结果】在最佳条件下,J3生长菌株对Pb~(2+)和Zn~(2+)的去除率分别为92.2%和87.7%;干菌体对Pb~(2+)和Zn~(2+)去除率分别为72.6%和23.8%,反应动力学模型研究表明,生长菌株吸附过程中吸附速率受重金属浓度影响,对Zn~(2+)吸附主要为颗粒内扩散作用;干菌体对Pb~(2+)吸附推测为膜扩散和颗粒内扩散作用,而对Zn~(2+)的吸附则由膜扩散控制。根据形态特征和系统发育分析,J3初步鉴定为虫生轮枝菌。【结论】生长菌株吸附效果好于干菌体,二级动力学方程拟合结果可为生物吸附反应器的设计提供理论参考和实践指导。
作者机构：
[陈全超; 耿勤; 李科林; 冉宇光; 张平; 廖柏寒] Institute of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, China
通讯机构：
[Zhang, P.] I;Institute of Environmental Science and Engineering, China
