摘要：
【目的】针对湖南资兴铅锌矿污染问题,筛选本土耐性菌株用作生物修复。【方法】供试菌株J3筛选自湖南资兴铅锌矿区的尾砂矿矿渣,利用正交实验分析其生长菌株及干菌体最佳吸附条件以优化其吸附效果,同时对相关数据进行动力学拟合以探讨其吸附机理,最后结合形态观察和18S r RNA基因序列分析对其进行分类鉴定。【结果】在最佳条件下,J3生长菌株对Pb~(2+)和Zn~(2+)的去除率分别为92.2%和87.7%;干菌体对Pb~(2+)和Zn~(2+)去除率分别为72.6%和23.8%,反应动力学模型研究表明,生长菌株吸附过程中吸附速率受重金属浓度影响,对Zn~(2+)吸附主要为颗粒内扩散作用;干菌体对Pb~(2+)吸附推测为膜扩散和颗粒内扩散作用,而对Zn~(2+)的吸附则由膜扩散控制。根据形态特征和系统发育分析,J3初步鉴定为虫生轮枝菌。【结论】生长菌株吸附效果好于干菌体,二级动力学方程拟合结果可为生物吸附反应器的设计提供理论参考和实践指导。
作者机构：
[陈全超; 耿勤; 李科林; 冉宇光; 张平; 廖柏寒] Institute of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, China
通讯机构：
[Zhang, P.] I;Institute of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha, China
作者机构：
[张平; 李科林; Qiu Y.; 廖柏寒] Institute of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
通讯机构：
[Zhang, P.] I;Institute of Environmental Science and Engineering, Central South University of Forestry and Technology, China
通讯机构：
[Wu, Xiaofu] C;Cent South Univ Forestry & Technol, Res Ctr Environm Sci & Engn, Changsha 410004, Hunan, Peoples R China.
关键词：
vertical-flow constructed wetlands;plant intercropping model;warm seasonal plant model
摘要：
Subtropical climatic conditions can contribute to the death of the aerial parts of constructed wetland plants in winter. This presents a barrier to the widespread application of constructed wetland and is an issue that urgently needs to be solved. Three contrasting experiments, the plant-intercropping model (A), the warm-seasonal plant model (B), and the non-plant model (C), were studied in terms of their efficiency in removing pollutants, and the change in root structure of plants in the plant-intercropping model within the vertical-flow constructed wetlands. The results indicate that model A was able to solve the aforementioned problem. Overall, average removal rates of three pollutants (CODCr, total nitrogen (TN) and total phosphorous (TP)) using model A were significantly higher than those obtained using models B and C (P<0.01). Moreover, no significant differences in removal rates of the three pollutants were detected between A and B during the higher temperature part of the year (P > 0.05). Conversely, removal rates of the three pollutants were found to be significantly higher using model A than those observed using model B during the lower temperature part of the year (P<0.01). Furthermore, the morphologies and internal structures of plant roots further demonstrate that numerous white roots, whose distribution in soil was generally shallow, extend further under model A. The roots of these aquatic plants have an aerenchyma structure composed of parenchyma cells, therefore, roots of the cold-seasonal plants with major growth advantages used in A were capable of creating a more favorable vertical-flow constructed wetlands mediamicroenvironment. In conclusion, the plant-intercropping model (A) is more suitable for use in the cold environment experienced by constructed wetland during winter.
作者机构：
[朱健] Institute of Environmental Science and Engineering Research, Central South University of Forestry and Technology, Changsha 410004, China. zhujian198312@163.com;[吴庆定] Institute of Processing Technologies of Materials, Central South University of Forestry and Technology, Changsha 410004, China;[李科林; 张伟丽; 王平; 雷明婧] Institute of Environmental Science and Engineering Research, Central South University of Forestry and Technology, Changsha 410004, China
会议名称：
Global Conference on Civil, Structural and Environmental Engineering / 3rd International Symp on Multi-field Coupling Theory of Rock and Soil Media and its Applications
会议时间：
OCT 20-21, 2012
会议地点：
China Three Gorges Univ, Yichang, PEOPLES R CHINA
