期刊论文

中文

微生物学通报

0253-2654

2015

42

7

1224-1233

10.13344/j.microbiol.china.140709

(No.2012BAC09B03):国家“十二五”科技支撑计划项目 (No.2012GS430203):国家“十二五”科技惠民计划项目 (No.10k082):湖南省高校科技创新平台开放基金项目 (No.2310006):湖南省环境科学与工程重点学科建设项目

本校为第一机构

【目的】针对湖南资兴铅锌矿污染问题,筛选本土耐性菌株用作生物修复。【方法】供试菌株J3筛选自湖南资兴铅锌矿区的尾砂矿矿渣,利用正交实验分析其生长菌株及干菌体最佳吸附条件以优化其吸附效果,同时对相关数据进行动力学拟合以探讨其吸附机理,最后结合形态观察和18S r RNA基因序列分析对其进行分类鉴定。【结果】在最佳条件下,J3生长菌株对Pb~(2+)和Zn~(2+)的去除率分别为92.2%和87.7%;干菌体对Pb~(2+)和Zn~(2+)去除率分别为72.6%和23.8%,反应动力学模型研究表明,生长菌株吸附过程中吸附速率受重金属浓度影响,对Zn~(2+)吸附主要为颗粒内扩散作用;干菌体对Pb~(2+)吸附推测为膜扩散和颗粒内扩散...

[Objective] The aim of this study is to screen lead-zinc tolerant fungus for the bioremediation of heavy metal pollution in Zixing, Hunan. [Methods] The fungus J3 which was isolated from the local tailing of lead-zinc deposit was used. Orthogonal test was carried out for studying adsorption conditions optimization and biosorption mechanism was discussed by kinetics simulation. And morphological characteristics and 18S rRNA gene sequence analysis were used for identification. [Resultsl Under the optimal conditions, the results showed that the re...