摘要：
Potassium (K) plays a pivotal role in influencing the structure and function of soil microbial communities, thereby influencing soil multifunctionality. Researches on various fertilization practices for Phoebe bournei has primarily focused on microbial communities. However, the mechanism of functional potential of microbe in mediating the influence of K on soil multifunctionality remains insufficiently elucidated. Here, the experiment included five K additions (CK, 0 g; K1, 60 g; K2, 120 g; K3, 180 g; and K4, 240 g per plant) in P. bournei young plantations via 16S rRNA sequencing and quantitative microbial element cycling (QMEC) smart chip technology to investigate the impacts of K additions on rhizosphere soil bacterial community attributes, nutrient cycling genes (carbon, nitrogen, phosphorus), and soil multifunctionality. K additions decreased bacterial diversity, while enhancing the abundance of genes involved in C degradation, including those related to labile and recalcitrant C, as well as N cycling, P cycling, and soil multifunctionality. Comparatively, K1 and K2 additions had slight effects on soil multifunctionality, bacterial communities and the abundance of C, N and P cycling genes. PLS-PM results demonstrated that K additions improve soil multifunctionality indirectly by altering bacterial community structure and network complexity, as well as the functional potential linked to N and P cycling. Additionally, soil abiotic factors are the was the core predictor for maintaining soil multifunctionality. All in all, soil properties and bacterial functional attributes together drive soil multifunctionality in response to K additions. These findings highlight that adequate K fertilizer may maintain soil multifunctionality, and regulate nutrient cycling and bacterial functions in P. bournei young plantations.
Potassium (K) plays a pivotal role in influencing the structure and function of soil microbial communities, thereby influencing soil multifunctionality. Researches on various fertilization practices for Phoebe bournei has primarily focused on microbial communities. However, the mechanism of functional potential of microbe in mediating the influence of K on soil multifunctionality remains insufficiently elucidated. Here, the experiment included five K additions (CK, 0 g; K1, 60 g; K2, 120 g; K3, 180 g; and K4, 240 g per plant) in P. bournei young plantations via 16S rRNA sequencing and quantitative microbial element cycling (QMEC) smart chip technology to investigate the impacts of K additions on rhizosphere soil bacterial community attributes, nutrient cycling genes (carbon, nitrogen, phosphorus), and soil multifunctionality. K additions decreased bacterial diversity, while enhancing the abundance of genes involved in C degradation, including those related to labile and recalcitrant C, as well as N cycling, P cycling, and soil multifunctionality. Comparatively, K1 and K2 additions had slight effects on soil multifunctionality, bacterial communities and the abundance of C, N and P cycling genes. PLS-PM results demonstrated that K additions improve soil multifunctionality indirectly by altering bacterial community structure and network complexity, as well as the functional potential linked to N and P cycling. Additionally, soil abiotic factors are the was the core predictor for maintaining soil multifunctionality. All in all, soil properties and bacterial functional attributes together drive soil multifunctionality in response to K additions. These findings highlight that adequate K fertilizer may maintain soil multifunctionality, and regulate nutrient cycling and bacterial functions in P. bournei young plantations.
期刊：
Ecology and Evolution,2025年15(3):e71034- ISSN：2045-7758
通讯作者：
Huashun Xiao
作者机构：
[Shisheng Long; Xuefeng He; Siqi Zeng; Huashun Xiao] Faculty of Forestry, Central South University of Forestry and Technology, Changsha, Hunan, China
通讯机构：
[Huashun Xiao] F;Faculty of Forestry, Central South University of Forestry and Technology, Changsha, Hunan, China
关键词：
basal area;canopy closure;maximum density line;self‐regulatory;self‐thinning
摘要：
The self-thinning rule in forest stands is fundamental to the development of density management strategies, as it determines the maximum stand density achievable for a given tree size. Accurate modeling of the maximum density line is crucial, but selecting representative data points for this purpose remains a challenge. Using 18 years of data from five Cunninghamia lanceolata plantations with varying initial planting densities, this study investigated whether relationships between mean tree basal area (g) and height (H) can reveal forest developmental stages and identify when stands begin self-thinning and reach maximum density. Our results showed a significant linear relationship (p < 0.05) between g and H after self-thinning was established, supporting the presence of self-regulatory growth mechanisms. These findings enabled the development of a novel sample selection method for constructing more accurate maximum density line models, outperforming traditional methods that rely on arbitrary thresholds. Additionally, we derived formulas to describe total stand basal area (G (1.0)) during different growth stages, revealing positive correlations with mean height during early growth and negative correlations with mean diameter during self-thinning. This research advances the understanding of self-thinning dynamics and provides practical tools for improving density management in plantation forestry.
摘要：
As an excellent variety of Camphora glanduliferum, Honganzhang has high essential oil content and can be used as industrial and pharmaceutical raw materials to extract essential oil, which has high development value. In this study, fresh branches and leaves of C. glanduliferum 'Honganzhang' were used as materials. After extracting C. glanduliferum 'Honganzhang' essential oils (CGHEO) by steam distillation, gas chromatography-mass spectrometry (GC-MS) was used to analyze its chemical components. On this basis, the antibacterial effect of CGHEO and its potential physiological mechanism were further explored. The results showed that the main components of CGHEO were Cineole (46.57%), alpha-terpineol (9.93%), Sabinene (8.02%), beta-terpinene (6.04%), and alpha-pinene (4.49%), these compounds can be used in medicine, food and other industries. In terms of antibacterial effect, CGHEO has significant antibacterial effects on three Gram-negative bacteria (Escherichia coli, Salmonella enteritidis, and Pseudomonas aeruginosa), and two Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus). CGHEO can reduce Na+K+-ATPase activity and SDH activity and further interfere with the operation of the bacterial respiratory chain, thereby achieving the effect of inhibiting bacterial reproduction. Based on the main chemical components contained in C. glanduliferum 'Honganzhang' and its remarkable antibacterial effect, this study is expected to provide a theoretical basis for the wide application of C. glanduliferum 'Honganzhang' in multiple fields such as medicine, health care, food, and industry.
通讯机构：
[He, JN ] C;Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China.
关键词：
M. Alternifolia;Provide shade;Photosynthesis;Chlorophyll;Endogenous hormone;Paraffin section
摘要：
In light of the detrimental effects of global warming, exacerbated by the intense sunlight and high temperatures prevalent during summer in southern China, on the growth of Melaleuca alternifolia (M. alternifolia) seedlings, this study sets out to examine the impact of various shading treatments on the growth and physiological attributes of M. alternifolia seedlings, with the aim of determining the optimum shading level. The seedling phase is of paramount importance in the growth cycle and regeneration process of M. alternifolia, as both excessive and insufficient light exposure can negatively influence seedling development. Four shading regimes (CK: no shading; C1: 35-45% shading; C2: 45-50% shading; C3: 60-70% shading) were imposed on M. alternifolia seedlings, following which observations were conducted after a 35-day period. The results reveal that the different shading treatments had a significant effect on the growth and physiological indicators of the seedlings, with the moderate shading treatment (C2) proving to be the most advantageous for seedling growth. Under the C2 treatment, an increase in chlorophyll content was observed in the leaves of the seedlings, accompanied by a decrease in malondialdehyde (MDA) content. Concurrently, there was a reduction in the activities of antioxidant enzymes, namely superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Furthermore, the levels of abscisic acid (ABA) and gibberellic acid (GA3) declined, while the concentrations of auxin (IAA), salicylic acid (SA), and cytokinin (CTK) ascended. Leaf anatomical analysis disclosed that, in comparison to the control group, the thickness of the lower epidermis and palisade tissue in the leaves of the seedlings subjected to the C2 treatment exhibited a significant reduction. Additionally, an increase in the number of spongy tissue cells was noted, along with a marked increase in total leaf thickness. Consequently, cultivating M. alternifolia seedlings under shading conditions ranging from 45 to 50% is deemed more favorable. The findings of this study offer novel insights into the adaptive responses of M. alternifolia seedlings to varying light intensities and provide theoretical guidance for the cultivation and management of M. alternifolia seedlings in the southern regions of China.
作者机构：
[Lu, Mengqi; Xiao, Zhun; Lu, Kaizheng; Zeng, Yanling; Wang, Jinfeng; Zhou, Junqin; Tan, Xiaofeng; Gu, Yiyang; Zeng, YL] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Peoples R China.;[Xiao, Zhun] Changsha Social Work Coll, Sch Foreign Languages, Changsha 410004, Peoples R China.;[Zeng, Yanling; Tan, Xiaofeng; Zeng, YL] Cent South Univ Forestry & Technol, Coll Forestry, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Peoples R China.;[Lu, Mengqi; Xiao, Zhun; Lu, Kaizheng; Zeng, Yanling; Wang, Jinfeng; Zhou, Junqin; Tan, Xiaofeng; Gu, Yiyang; Zeng, YL] Cent South Univ Forestry & Technol, Camellia Oil Tree Res Acad, Changsha 410004, Peoples R China.;[Lu, Mengqi] Hunan Acad Forestry, Changsha 410004, Peoples R China.
通讯机构：
[Tan, XF ; Zeng, YL] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Coll Forestry, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Camellia Oil Tree Res Acad, Changsha 410004, Peoples R China.
关键词：
Camellia Oleifera;Camellia lanceoleosa;Mitochondrial genome;De novo assembly;Phylogenetic analysis
摘要：
Camellia-oil trees are economically valuable, oil-rich species within the genus Camellia, family Theaceae. Among these species, C. oleifera, a member of Section Oleifera in the genus, is the most extensively cultivated in China. In this study, we assembled the mitochondrial genomes (mitogenomes) of two Camellia species, namely C. oleifera and C. lanceoleosa. These two species are closely related and belong to the same genus and section, with C. oleifera being hexaploid and C. lanceoleosa being diploid. The mitogenome of C. oleifera is comprised of 1,039,838 base pairs (bp), and C. lanceoleosa is comprised of 934,155bp. Both genomes exhibit a multipartite genome structure, which is supported by our PCR experiments. We conducted codon usage and RNA editing site analysis on these two mitogenomes, which showed highly consistent results. However, analysis of repetitive sequences and mitochondrial plastid sequences (MTPTs) revealed differences between the two mitogenomes. Phylogenetic analysis indicated that these two species clustered together, suggesting a close evolutionary relationship. The collinearity analysis results showed extensive genome rearrangements in the mitogenomes of Camellia species. We successfully assembled the mitogenomes of C. oleifera and C. lanceoleosa, marking a significant advancement in understanding camellia-oil tree mitogenomes. Unlike circular mitogenomes reported before, our research confirms multiple-branched configurations in these two species. This sheds light on mitogenome structural complexities and contributes to our understanding of evolutionary processes. Additionally, these results enrich Camellia genetic resources and expand our knowledge of mitogenome variation.
通讯机构：
[Tang, T ] C;Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China.;Natl Forestry & Grassland Adm Forest Resources Man, Key Lab, Changsha 410004, Peoples R China.
关键词：
Muntiacus reevesi;suitability habitat;south china tiger;MaxEnt;climate change
摘要：
Climate change and human disturbance are critical factors affecting the habitat distribution of wild animals, with implications for management strategies such as protecting migration corridors, habitat restoration, and species conservation. In the Hupingshan National Nature Reserve (NNR), Reeve's muntjac (Muntiacus reevesi) is a key prey species for the South China tiger (Panthera tigris amoyensis), which is extinct in the wild and targeted for reintroduction by the Chinese government. Thus, understanding the habitat distribution and abundance of Reeve's muntjac is essential to ensure the survival and sustainability of reintroduced tiger populations. Despite significant conservation efforts, the impacts of climate change and human disturbance on Reeve's muntjac habitat distribution in Hupingshan NNR remain unclear, though these factors could necessitate adaptive tiger management strategies due to shifts in prey abundance. In this study, we employed an optimized MaxEnt model to assess current habitat distribution and identify key environmental variables influencing the habitat distribution of Reeve's muntjac. Assuming non-climatic environmental factors will remain constant over the next century, we projected future habitat distribution under two shared socioeconomic pathways (SSP126 and SSP585) for the mid-century (2050s) and the late-century (2090s). Comparative analyses of current and projected habitat areas revealed potential impacts of climate change on this species. MaxEnt outputs classified habitat suitability into high, medium, and low levels. Results showed that climatic and human disturbance factors contributed 35.2% and 49.4% to habitat suitability, respectively. Under the SSP126 scenario, habitats with decreased suitability covered 0 km(2) in the 2050s, expanding slightly to 4.2 km(2) in the 2090s, while those with increased suitability spanned 491.1 km(2) (2050s) and 463.2 km(2) (2090s). Under the SSP585 scenario, habitats with decreased suitability covered 10.2 km(2) in the 2050s and 431.8 km(2) in the 2090s. Habitats with increased suitability were comparatively smaller under SSP585, covering 162.0 km(2) (2050s) and 1.1 km(2) (2090s). These findings suggest that while mid-century climate projections (SSP126 and SSP585) may support Reeve's muntjac's survival, habitat loss is projected by 2090s (SSP126). Thus, future climate change may lead to decreased habitat suitability and increased fragmentation, raising extinction risks for Reeves's muntjac. Mitigating these effects could involve establishing migration corridors, minimizing human disturbances, and potentially supplementing prey populations with captive-bred prey. Such measures are essential to support the South China tiger reintroduction plan and help ensure that prey availability remains sufficient for sustaining reintroduced tiger populations.
摘要：
BACKGROUND: Camellia oleifera Abel. is one of the four major woody oil species whose seeds produce high-grade edible oil. In recent years, the planting area of Camellia oleifera is increasing in China. However, in the process of cultivation, due to the high fruit load, the Camellia oleifera tree has small fruit and poor quality. In previous studies, we explored the optimal leaf-fruit ratio. In this study, the changes of molecular regulation mechanism of Camellia oleifera under different leaf-fruit ratios were revealed by combining physiological indexes with transcriptome data. RESULT: The physiological results showed that the content of MDA and starch in leaves increased significantly with the decrease in the leaf-to-fruit ratio. The results of transcriptome showed that there was a close relationship between leaf-fruit ratio and phenylpropanoid biosynthesis pathway. With the decrease of leaf-fruit ratio, the expression of genes related to lignin and flavonoid biosynthesis increased significantly, which promoted the synthesis of lignin and flavonoid. CONCLUSIONS: Combining physiological indicators and transcriptomics, we demonstrated that leaf-fruit ratio can significantly affect the normal growth of plants. When the fruit load is too high, the fruit as a 'sink' will consume a large amount of nutrients in the plant body and promote the biosynthesis of lignin and flavonoids in the body. The results provide a more reliable scientific basis for the study of cultivation and management of Camellia oleifera.
摘要：
Liquid crystal monomers (LCMs), the integral components in the manufacture of digital displays, have engendered environmental concerns due to extensive utilization and intensive emission. Despite their prevalence and ecotoxicity, the LCM impacts on plant growth and agricultural yield remain inadequately understood. In this study, we investigated the specific response mechanisms of tobacco, a pivotal agricultural crop and model plant, to four representative LCMs (2OdF3B, 5CB, 4PiMeOP, 2BzoCP) through integrative molecular and physiological approaches. The findings reveal specific impacts, with 4PiMeOP exerting the most pronounced effects, followed by 2BzoCP, 5CB, and 2OdF3B. LCM exposure disrupts the photosynthetic apparatus, exacerbating reactive oxygen species (ROS) levels in leaves, which in turn triggers the upregulation of antioxidative enzymes and the synthesis of antioxidant substances. Additionally, LCMs strongly stimulate the expression of genes involved in abscisic acid (ABA) biosynthesis and signalling pathways. The AI-assisted meta-analysis implicates ABA as a critical regulator in the tobacco response to LCMs. Notably, exogenous application of ABA alleviates LCM-induced toxicities, highlighting the pivotal role of ABA in stress amelioration. Our study provides novel insights into the toxicity and tolerance mechanisms of LCMs in plants, shedding light on both their harmful effects on the ecosystems and potential adaptation responses. This is crucial to develop sustainable agricultural systems by reducing the negative environmental impacts caused by emerging organic pollutants.
期刊：
Journal of Medical Primatology,2025年54(2):e70003- ISSN：0047-2565
通讯作者：
Xiang, ZF
作者机构：
[Chen, Luwei; Zaman, Muhammad; Chen, Mei; Xiang, Zuofu; Wei, Fangyi] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.;[Yao, Hui] Key Lab Conservat Biol Shennongjia Golden Monkey, Shennongjia, Peoples R China.
通讯机构：
[Xiang, ZF ] C;Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.
关键词：
age classes;deaths;endemic;gender;physical wounds;sign of diseases
摘要：
BACKGROUND: Globally, approximately 60% of primate taxa are in danger of extinction and the population of 75% of primate species are declining in their natural habitats. The Endangered R. roxellana hubeiensis monkeys in Shennongjia National Park face threats due to their limited genetic diversity and restricted habitat. METHOD: Our study aimed to assess the health of these monkeys through focal animal sampling observations of 90 individuals from June 2023 to July 2024. Additionally, we compared the prevalence of health concerns on days with and without snow. We identified health issues in 32 individuals (46%), including wounds, signs suggesting disease, and deaths. RESULTS: Nine lactating females one juvenile and one infant had eye injuries. We noticed three females and one male exhibiting symptoms of eye diseases. We observed that one adult male and three females showed signs of respiratory disease, while three females exhibited signs of skin disease. We found that one adult male and three females indicated signs of respiratory disease and three females showed signs of skin disease. We found that seven monkeys of different ages, including adults, juveniles and infants, died from injuries, diseases and unknown causes. Respiratory and skin disease symptoms were more common on snowy days than on non-snowy days. Additionally, physical and eye injuries were also more prevalent on snowy days. CONCLUSIONS: In general, our study investigated the health status of Shennongjia golden snub-nosed monkeys and found signs of disease, wounds, and death. Our study also highlighted health issues in monkeys, which can help guide conservation efforts to ensure their survival in their natural habitats.
期刊：
Land Degradation & Development,2025年 ISSN：1085-3278
通讯作者：
Yan, WD;Dang, Peng;Dang, P
作者机构：
[Lei, Junjie; Yan, Wende] Cent South Univ Forestry & Technol, Coll Life & Environm Sci, Changsha, Peoples R China.;[Lei, Junjie; Yan, Wende; Dang, Peng; Dang, P] Natl Engn Lab Appl Technol Forestry & Ecol South C, Changsha, Peoples R China.;[Peng, Yuanying] Lewis Univ, Coll Arts & Sci, Romeoville, IL USA.;[Gao, Xiaoqian; Dang, Peng; Dang, P] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.;[Chen, Xiaoyong] Governors State Univ, Coll Arts & Sci, University Pk, IL USA.
通讯机构：
[Dang, P ; Yan, WD ] C;[Dang, P] N;Cent South Univ Forestry & Technol, Coll Life & Environm Sci, Changsha, Peoples R China.;Natl Engn Lab Appl Technol Forestry & Ecol South C, Changsha, Peoples R China.;Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.
关键词：
available nitrogen;karst ecosystem;microbial necromass;nitrogen sequestration;plantation
摘要：
Microbial necromass nitrogen (MNN) is increasingly recognized as a major source of soil N, playing a crucial role in N sequestration and sustaining N balance. However, quantitative data on the contribution of MNN to total N and its relationship with soil multifunctionality (SMF) in karst plantation ecosystems remain lacking. In this study, MNN (measured via amino sugar analysis) and SMF (calculated using the mean value method) were determined across five plantations and unafforested land (control) in the Wuling Mountains of subtropical China. The soil MNN content ranged from 1.02 to 1.67 g kg −1 , contributing 53.8% to 75.4% of the total N, with Cinnamomum camphora pure forest exhibiting the highest values among all stand types and control. The afforestation of Cinnamomum camphora enhanced soil nutrient provision, microbial activity, and biomass, increasing SMF by 54% compared to the control. The soil functional parameters were generally higher in the organic horizon than in the topsoil and subsoil, and the contribution of MNN to total N was greater in the subsoil. Enzyme stoichiometry ratios indicated that microbial P limitation was prevalent in both plantation and control soils, with MNN content positively correlated with C/P and N/P ratios ( p < 0.001). Increases in MNN were closely associated with SMF, encompassing nutrient provision, microbial activity, and biomass, with available N (AN) being a key predictor of MNN accumulation. The partial least squares path model revealed that nutrient provision contributed positively to MNN accumulation, and AN had a direct effect path coefficient of 0.72 on it ( p < 0.01). This work underscored the critical role of MNN in contributing to N sequestration and its potential to support ecosystem functioning in the karst region.
作者机构：
[Chen, Caihong; Luo, Ziyi; She, Jiyun; Wang, Yamin] Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China.;[Tong, Wenfu] Cent South Univ Forestry & Technol, Engn Res Ctr Forestry Equipment Hunan Prov, Changsha 410004, Peoples R China.;[Guo, Zexin] Cent South Univ Forestry & Technol, Coll Comp & Math, Changsha 410004, Peoples R China.
通讯机构：
[Chen, CH ] C;Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China.
关键词：
land use;carbon stocks;multi-scenario predictions;multiscale geographically weighted regression (MGWR);Changde
摘要：
In the context of the worldwide attention on climate change, examining how land use relates to the carbon sink functions of regions is essential. This research innovatively utilizes the 2000-2020 land use data of Changde City, integrating the PLUS and InVEST models to analyze spatiotemporal changes and predict scenarios. It also combines the parameter geodetector and multiscale geographically weighted regression model to dissect driving factor distributions and mechanisms, capture interactions and multiscale impacts, uncover underlying laws, pioneer new paths for similar studies, and support regional ecological sustainability. The results show that from 2000-2020, forest and arable land areas declined while construction land expanded, leading to a yij1,172,200-ton carbon storage reduction in Changde City. Carbon storage decreased under natural development and arable land protection scenarios but increased in the ecological scenario. The main drivers of carbon storage in Changde City are the DEM, slope, and annual average temperature, with their interactions enhancing spatial heterogeneity. Human activities, especially in mountains and urbanizing regions, negatively impact carbon storage. This study aids in optimizing land resource allocation, improving land use efficiency, and promoting coordinated and sustainable development in Changde City's ecological, economic, and social systems.
摘要：
Previous research has consistently shown that high-fat diet (HFD) consumption can lead to the development of colonic inflammation. Neohesperidin (NHP), a naturally occurring flavanone glycoside in citrus fruits, has anti-inflammatory properties. However, the efficacy and mechanism of NHP in countering prolonged HFD-induced inflammation remains unclear. In this study, rats on HFD were intragastrically administered (i.g.) with NHP for 12 consecutive weeks. Results indicate that this natural compound is effective in reducing colorectal inflammation at doses of 40-80 mg/kg body weight (BW) by i.g. administration, with significant decreases in inflammation markers such as TNF-α and IL-1β levels. It also improved intestinal mucosal tissue integrity and reduced HFD-stimulated colorectal inflammation via the JAK2/STAT3 pathway. Furthermore, intestinal microbiota sequencing results show that NHP intervention significantly downregulated the Firmicutes/Bacteroidetes ratio. This ratio is closely related to the preventive role in the context of glycolipid metabolism disorder. Compared with fecal cultures of rats from the HFD group, after 48 h in vitro fermentation, those from the NHP group had distinct microbiota composition and notably higher concentrations of SCFAs. Collectively, these observations suggest that 80 mg/kg BW NHP possesses biological activities in downregulating HFD-induced colorectal inflammation by regulating intestinal flora and promoting SCFAs formation.
作者机构：
[Zheng, Wei; Wu, Jinshui] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Inst Subtrop Agr, Changsha 410125, Peoples R China.;[Zheng, Wei; Wu, Jinshui] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Zheng, Wei; Yan, Wende] Natl Engn Lab Appl Technol Forestry & Ecol South C, Changsha 410004, Peoples R China.;[Zheng, Wei; Yan, Wende] Cent South Univ Forestry & Technol, Fac Life & Environm Sci, Changsha 410004, Peoples R China.;[Dang, Peng; Dang, P] Cent South Univ Forestry & Technol, Fac Forestry, Changsha 410004, Peoples R China.
通讯机构：
[Dang, P ] C;[Yan, WD ] N;Natl Engn Lab Appl Technol Forestry & Ecol South C, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Fac Life & Environm Sci, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Fac Forestry, Changsha 410004, Peoples R China.
关键词：
Nitrogen availability and cycling;Chinese fir plantations;MAT and MAP;Climatic gradient;Subtropical region
摘要：
Nitrogen (N) plays a vital role in the biogeochemical cycles of terrestrial ecosystems under the global climate change context. However, the effects of climate variables on soil N availability and cycling in Chinese fir plantations remain poorly understood. In this study, we evaluated how changes in mean annual temperature (MAT) and precipitation (MAP) affect soil N availability and cycling through field survey experiments conducted in Chinese fir plantation ecosystems across subtropical China. Our findings revealed that the increase in MAT and MAP from north to south across subtropical China resulted in higher soil organic matter inputs and changes in soil properties. These shifts, accompanied by a decrease in soil pH, drove alterations in soil N fractions, sources, and demands, particularly nitrate-N (NO 3 – ) and ammonia-N (NH 4 + ). Specifically, soil N availability increased (NO 3 – : NH 4 + > 1) in regions with the highest MAT and MAP, and N cycling strategy shifted from slow-closed acquiring to fast-open cycling. Mechanism model analyses indicated that MAT and MAP impact soil N availability and cycling by directly and indirectly altering soil properties and carbon fractions, as well as affecting the conversion between N fractions. Collectively, this study underscores the importance of MAT and MAP in shaping soil N dynamics by influencing soil variables in Chinese fir plantations and provides theoretical guidance for the sustainable management of future artificial forests. Furthermore, future research should further investigate the role of soil pH in regulating soil nutrient cycling under global climate change context.
Nitrogen (N) plays a vital role in the biogeochemical cycles of terrestrial ecosystems under the global climate change context. However, the effects of climate variables on soil N availability and cycling in Chinese fir plantations remain poorly understood. In this study, we evaluated how changes in mean annual temperature (MAT) and precipitation (MAP) affect soil N availability and cycling through field survey experiments conducted in Chinese fir plantation ecosystems across subtropical China. Our findings revealed that the increase in MAT and MAP from north to south across subtropical China resulted in higher soil organic matter inputs and changes in soil properties. These shifts, accompanied by a decrease in soil pH, drove alterations in soil N fractions, sources, and demands, particularly nitrate-N (NO 3 – ) and ammonia-N (NH 4 + ). Specifically, soil N availability increased (NO 3 – : NH 4 + > 1) in regions with the highest MAT and MAP, and N cycling strategy shifted from slow-closed acquiring to fast-open cycling. Mechanism model analyses indicated that MAT and MAP impact soil N availability and cycling by directly and indirectly altering soil properties and carbon fractions, as well as affecting the conversion between N fractions. Collectively, this study underscores the importance of MAT and MAP in shaping soil N dynamics by influencing soil variables in Chinese fir plantations and provides theoretical guidance for the sustainable management of future artificial forests. Furthermore, future research should further investigate the role of soil pH in regulating soil nutrient cycling under global climate change context.
作者机构：
[Qiu, Qi; Wu, Juntao; Wu, Guolong; Yuan, Deyi; Fan, Xiaoming] Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan Province, China;[Qiu, Qi; Wu, Juntao; Wu, Guolong; Yuan, Deyi; Fan, Xiaoming] Key Laboratory of Non-Wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha, Hunan, China;[Tian, Xiaoming] Hunan Botanical Garden, Changsha, China
摘要：
BACKGROUND: Many plants, including those commonly found in the Fagaceae family, produce more flowers and ovules than mature fruits and seeds. In Castanea henryi, an ovary contains 16-24 ovules, but only one develops into a seed. The other ovules abort or otherwise fail to fully develop, but the reason for this is unknown. Such a strict reproductive screening mechanism is rare in plants. METHODS: In this study, controlled pollination scheme were adopted, and conventional paraffin embedding and semi-thin sectioning techniques, followed by microscopy, were used for cytological studies of ovule development in C. henryi. RESULTS: Pollination affected not only the process of ovule development, but also the proportion of ovules that formed mature embryo sacs. Approximately 53.53% of the ovules in the pollinated treatment developed normally, while only 16.55% of the ovules in the unpollinated treatment developed into mature embryo sacs with a seven-cell, eight-nucleated structure. Failure to form mature embryo sacs and the abnormal divisions of the zygote, respectively, were the reasons for the pre- and post-fertilization ovule failures. Our findings not only provide basic information on the reproductive biology and also information on seed production of C. henryi.
通讯机构：
[Chen, HG; Zou, JJ ] H;Hubei Univ Sci & Technol, Natl Forestry & Grassland Adm Engn Res Ctr Osmanth, Xianning, Peoples R China.;Hubei Univ Sci & Technol, Osmanthus Innovat Ctr Natl Engn Res Ctr Floricultu, Xianning, Peoples R China.;Xianning Res Acad Ind Technol Osmanthus Fragrans, Res Ctr Osmanthus Fragrans, Xianning, Peoples R China.
关键词：
Osmanthus fragrans;reference lncRNAs;flowering stage;abiotic stress;hormone treatment;metal ion treatment
摘要：
INTODUCTION: Osmanthus fragrans, a well-regarded traditional flower in China, holds extensive applications in gardening, food, cosmetics, and traditional Chinese medicine. Despite its importance, research on long non-coding RNAs (lncRNAs) in O. fragrans has been constrained by the absence of reliable reference genes (RGs). METHODS: We employed five distinct algorithms, i.e., delta-Ct, NormFinder, geNorm, BestKeeper, and RefFinder, to evaluate the expression stability of 17 candidate RGs across various experimental conditions. RESULTS AND DISCUSSION: The results indicated the most stable RG combinations under different conditions as follows: cold stress: lnc00249739 and lnc00042194; drought stress: lnc00042194 and lnc00174850; salt stress: lnc00239991 and lnc00042194; abiotic stress: lnc00239991, lnc00042194, lnc00067193, and lnc00265419; ABA treatment: lnc00239991 and 18S; MeJA treatment: lnc00265419 and lnc00249739; ethephon treatment: lnc00229717 and lnc00044331; hormone treatments: lnc00265419 and lnc00239991; Al(3+) treatment: lnc00087780 and lnc00265419; Cu(2+) treatment: lnc00067193 and 18S; Fe(2+) treatment: lnc00229717 and ACT7; metal ion treatment: lnc00239991 and lnc00067193; flowering stage: lnc00229717 and RAN1; different tissues: lnc00239991, lnc00042194, lnc00067193, TUA5, UBQ4, and RAN1; and across all samples: lnc00239991, lnc00042194, lnc00265419 and UBQ4. The reliability of these selected RGs was further validated by analyzing the expression patterns of lnc00003036, lnc00126603, and lnc00250780. This study represents the first comprehensive evaluation of lncRNA RG stability in O. fragrans, significantly enhancing the accuracy and depth of lncRNA expression analyses in this species, contributing to advancements in plant stress resistance breeding and improving environmental adaptability.
期刊：
International Journal of Biological Macromolecules,2025年289:138988 ISSN：0141-8130
通讯作者：
Li, H
作者机构：
[Li, He; Li, H] Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China.;Minist Educ, Key Lab Nonwood Forest Cultivat & Conservat, Changsha 410004, Peoples R China.;Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Peoples R China.
通讯机构：
[Li, H ] C;Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China.
关键词：
Small GTPase;Vesicle trafficking;Protein-protein interaction;Pathogenicity;C. fructicola
摘要：
Small GTPase of the Rab family functions as molecular switch in vesicle trafficking, regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). In our ongoing efforts to study the pathogenesis of Colletotrichum fructicola, the causal agent of anthracnose in edible-oil plant Camellia oleifera, we identified CfRab6 as the Rab GTPase and characterized its roles in C. fructicola. Consistent with our hypothesis, targeted gene deletion revealed that the ΔCfrab6 mutant displays defects in vesicle trafficking, including endocytosis and autophagy. These combined effects led to the impairments in growth, conidia, and pathogenicity. Moreover, we demonstrated the critical importance of the GDP/GTP motifs are crucial for the normal function of CfRab6. Additionally, our findings demonstrated that CfRic1 and CfRgp1 act as conserved GEFs for CfRab6, supported by their interactions with CfRab6 and the partial restoration of the active GTP-bound CfRab6, which alleviated phenotypic defects in the ΔCfric1 and ΔCfrgp1 mutants. In conclusion, our study sheds new light on the significance of CfRab6-mediated vesicle trafficking in the physiology and pathogenicity of C. fructicola, which might offer new potential targets for the management of anthracnose disease.
Small GTPase of the Rab family functions as molecular switch in vesicle trafficking, regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). In our ongoing efforts to study the pathogenesis of Colletotrichum fructicola, the causal agent of anthracnose in edible-oil plant Camellia oleifera, we identified CfRab6 as the Rab GTPase and characterized its roles in C. fructicola. Consistent with our hypothesis, targeted gene deletion revealed that the ΔCfrab6 mutant displays defects in vesicle trafficking, including endocytosis and autophagy. These combined effects led to the impairments in growth, conidia, and pathogenicity. Moreover, we demonstrated the critical importance of the GDP/GTP motifs are crucial for the normal function of CfRab6. Additionally, our findings demonstrated that CfRic1 and CfRgp1 act as conserved GEFs for CfRab6, supported by their interactions with CfRab6 and the partial restoration of the active GTP-bound CfRab6, which alleviated phenotypic defects in the ΔCfric1 and ΔCfrgp1 mutants. In conclusion, our study sheds new light on the significance of CfRab6-mediated vesicle trafficking in the physiology and pathogenicity of C. fructicola, which might offer new potential targets for the management of anthracnose disease.
摘要：
The degree of lignification in the spring shoots of Camellia oleifera significantly affects the quality of grafted scions. However, the regulatory mechanisms of lignification are poorly understood. This study explored the effects of exogenous gibberellin A 3 (GA 3 ) on lignification in the spring shoots of C. oleifera . Five-year-old C. oleifera (cultivar, Huaxin) were subjected to treatments with different concentrations of GA 3 and uniconazole. The GA effect was assessed in terms of the morphology, physiology, and molecular levels. The results indicate that exogenous GA 3 markedly decreased lignin content. Transcriptomic and metabolomic analyses revealed that exogenous GA 3 inhibited the expression of genes associated with the cytokinin signaling pathway, specifically downregulating histidine kinases ( HK3 , HK4 , and HK2 ) and response regulators ( RR23 , RR26 , and RR4 ), as well as genes involved in lignin synthesis, including hydroxycinnamoyltransferase ( HCT ), ferulate 5-hydroxylase ( F5H ), and peroxidase ( PER ). The concentration of intermediates involved in monolignol synthesis, such as sinapaldehyde, was altered in response to GA treatment. These findings revealed that the application of GA 3 may be an efficient strategy to regulate the spring shoot growth of C. oleifera , hence enhancing scion quality.
The degree of lignification in the spring shoots of Camellia oleifera significantly affects the quality of grafted scions. However, the regulatory mechanisms of lignification are poorly understood. This study explored the effects of exogenous gibberellin A 3 (GA 3 ) on lignification in the spring shoots of C. oleifera . Five-year-old C. oleifera (cultivar, Huaxin) were subjected to treatments with different concentrations of GA 3 and uniconazole. The GA effect was assessed in terms of the morphology, physiology, and molecular levels. The results indicate that exogenous GA 3 markedly decreased lignin content. Transcriptomic and metabolomic analyses revealed that exogenous GA 3 inhibited the expression of genes associated with the cytokinin signaling pathway, specifically downregulating histidine kinases ( HK3 , HK4 , and HK2 ) and response regulators ( RR23 , RR26 , and RR4 ), as well as genes involved in lignin synthesis, including hydroxycinnamoyltransferase ( HCT ), ferulate 5-hydroxylase ( F5H ), and peroxidase ( PER ). The concentration of intermediates involved in monolignol synthesis, such as sinapaldehyde, was altered in response to GA treatment. These findings revealed that the application of GA 3 may be an efficient strategy to regulate the spring shoot growth of C. oleifera , hence enhancing scion quality.
作者机构：
[Chen, Yi; Su, Qianqian; Zhang, Chen; Xiang, Zuofu; Liu, Yang] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Hunan, Peoples R China.;[Guo, Cheng; Yu, Yang; Chen, Yixin] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Changsha, Hunan, Peoples R China.;[Yue, Ling] Panzhihua Anim Dis Prevent & Control Ctr, Panzhihua, Sichuan, Peoples R China.
通讯机构：
[Su, QQ; Xiang, ZF ] C;Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Hunan, Peoples R China.
摘要：
The unique environment of the Qinghai-Tibetan Plateau provides a great opportunity to study how primate intestinal microorganisms adapt to ecosystems. The 16S rRNA gene amplicon and metagenome analysis were conducted to investigate the correlation between gut microbiota in primates and other sympatric animal species living between 3600 and 4500 m asl. Results showed that within the same geographical environment, Macaca mulatta and Rhinopithecus bieti exhibited a gut microbiome composition similar to that of Tibetan people, influenced by genetic evolution of host, while significantly differing from other distantly related animals. The gut microbiota of plateau species has developed similar strategies to facilitate their hosts’ adaptation to specific environments, including broadening its dietary niche and enhancing energy absorption. These findings will enhance our comprehension of the significance of primate gut microbiota in adapting to specific habitats. Analyses of gut microbiomes from sympatric animals of the Qinghai-Tibetan Plateau and comparison with Tibetan human gut microbiomes reveals the impact of host phylogeny on gut microbiota composition.
作者机构：
[Xiong, Chi] Guangxi Zhuang Autonomous Reg & Chinese Acad Sci, Guangxi Inst Bot, Guangxi Key Lab Plant Conservat & Restorat Ecol Ka, Guilin 541006, Peoples R China.;[Yang, Yu-xin; Sun, Fu-zhang] Guangxi Normal Univ, Coll Life Sci, Guilin 541006, Guangxi, Peoples R China.;[Wu, Lei] Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China.
通讯机构：
[Wu, L ] C;Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China.
关键词：
Hechi;flora of Guangxi;Karst cave;new taxon;Rubiaceae;Spiradiclis
摘要：
A new species of Rubiaceae, Spiradiclis hechiensis, is described and illustrated from a cave in the Karst region of Northwest Guangxi, China. Morphologically, it is related to S. pauciflora in the shape and color of the corolla. It can be easily distinguished by the shape of leaf blades, bracts, and calyx lobes, the number of secondary veins and flowers, as well as the size of capsules and seeds. At present, only one population has been confirmed to have approximately 50-100 mature individuals from the type locality. According to the IUCN Red List Categories and Criteria, it is provisionally assessed as Critically Endangered [CR B1+2ab (iii)].
摘要：
(1) Background: As population growth accelerates, unsustainable practices such as excessive cutting and burning of desert plants in the transition zones between deserts and oases have led to widespread vegetation loss. (2) Methods: The experiment was conducted in the oasis transition zone on the southern edge of the Taklamakan Desert from 2010 to 2023 year. Among the treatments included a control group (CK), cutting in spring (CS), cutting in fall (CF), burning in spring (BS), and flood water irrigation (FI). We used high-throughput sequencing to determine soil microbial composition and diversity and routine laboratory methods to determine soil physical and chemical properties and enzyme activities. (3) Results: No significant differences in bacterial alpha diversity (Chao1, Dominance, Observed_features, Pielou_e, Shannon, and Simpson) across the different long-term disturbance patterns. In fungi, the CK treatment showed significantly higher Chao1, Shannon, and Observed_features indices compared to BS and FI. Principal component analysis revealed a substantial reduction in bacterial community diversity in BS compared to FI, while fungal communities were lower in CK and CS compared to BS, CF, and FI; (4) Conclusions: Soil moisture content, electrical conductivity, organic carbon, and the activity of the enzyme cellobiohydrolase as key factors shaping the bacterial community. For fungi, organic carbon and the beta-1,4-glucosidase enzyme were the main drivers.
