摘要：
Creation of eco-friendly and high-performance nanocomposites has become one of the most widely concerned focuses. Regenerated cellulose aerogels (RCAs), the typical green sustainable 3D cellulose products, have numerous merits including large surface area, high porosity, low density, high mechanical strength, 3D network structure and abundant oxygen-containing groups, which make them ideal candidates as green matrices to support various active nanomaterials for the development of novel functional nanocomposites. Therefore, RCAs open up a new promising avenue to create novel enticing materials with desired and tunable properties. Also, it is of great significance to search exact modification or adulteration technologies to create RCAs-based nanocomposites for advanced applications. In addition, to make RCAs more applicable and valuable, a deep understanding of the relationship between the structure (mainly dependent on the dissolution, regeneration and drying techniques) and property of RCAs is also necessary. Here, this review article highlights recent advances in the field of RCAs-based functional nanocomposites. The synthetic processes and mechanisms and representative physicochemical properties are also emphasized. We hope that this review work could play a certain guiding role for the study and creation of green RCAs-based functional materials and stimulate a wider range of studies and collaborations, leading to significant progress in this area.
摘要：
In this report, self-sterility in Camellia oleifera was explored by comparing structural and statistical characteristics following self-pollination (SP) and cross-pollination (CP). Although slightly delayed pollen germination and pollen tube growth in selfed ovaries compared to crossed ovaries was observed, there was no significant difference in the percentages of pollen that germinated and pollen tubes that grew to the base of the style. There was also no difference in morphological structure after the two pollination treatments. However, the proportions of ovule penetration and double fertilization in selfed ovules were significantly lower than in crossed ovules, indicating that a prezygotic late-acting self-incompatible mechanism may exist in C. oleifera. Callose deposition was observed in selfed abortive ovules, but not in normal. Ovules did not show differences in anatomic structure during embryonic development, whereas significant differences were observed in the final fruit and seed set. In addition, aborted ovules in selfed ovaries occurred within 35 days after SP and prior to zygote division. However, this process did not occur continuously throughout the life cycle, and no zygotes were observed in the selfed abortive ovules. These results indicated that the self-sterility in C. oleifera may be caused by prezygotic late-acting self-incompatibility (LSI).
摘要：
<jats:sec><jats:label /><jats:p>Small ubiquitin‐related modifier‐1 (<jats:styled-content style="fixed-case">SUMO</jats:styled-content>‐1) modification has been implicated in many important cellular processes, including cell cycle progression, apoptosis, cellular proliferation, and development, but its role in all‐<jats:italic>trans</jats:italic>‐retinoic acid (<jats:styled-content style="fixed-case">ATRA</jats:styled-content>)‐induced differentiation processes of cancer cells remains unclear. Here, we report for the first time that <jats:styled-content style="fixed-case">ATRA</jats:styled-content>‐induced differentiation of leukemia and osteosarcoma is accompanied by a decrease in the level of <jats:styled-content style="fixed-case">SUMO</jats:styled-content>‐1 protein. Our results also demonstrated that depletion or inhibition of <jats:styled-content style="fixed-case">SUMO</jats:styled-content>‐1 blocks <jats:styled-content style="fixed-case">ATRA</jats:styled-content>‐induced differentiation, suggesting that <jats:styled-content style="fixed-case">SUMO</jats:styled-content>‐1 is critical for the differentiation effect of <jats:styled-content style="fixed-case">ATRA</jats:styled-content>. Further studies indicated that <jats:styled-content style="fixed-case">SUMO</jats:styled-content>‐1‐promoted <jats:styled-content style="fixed-case">ATRA</jats:styled-content>‐induced differentiation might be associated with the stabilization of retinoic acid receptorα (<jats:styled-content style="fixed-case">RAR</jats:styled-content>α), protecting it from degradation. Moreover, our results suggested that Lys399 is a major site for <jats:styled-content style="fixed-case">SUMO</jats:styled-content>‐1 conjugation of <jats:styled-content style="fixed-case">RAR</jats:styled-content>α. We also found that <jats:styled-content style="fixed-case">RAR</jats:styled-content>α enhanced the transcription of its target genes, which might also contribute to the enhanced differentiating effects of <jats:styled-content style="fixed-case">ATRA</jats:styled-content>; however, mutation of Lys399 of <jats:styled-content style="fixed-case">RAR</jats:styled-content>α inhibits the extents of both <jats:styled-content style="fixed-case">SUMO</jats:styled-content>‐1 modification and <jats:styled-content style="fixed-case">ATRA</jats:styled-content>‐induced differentiation. Together, these results indicate that <jats:styled-content style="fixed-case">SUMO</jats:styled-content>‐1 modification of <jats:styled-content style="fixed-case">RAR</jats:styled-content>α is a potent mechanism for balancing proliferation and differentiation by controlling the stability of <jats:styled-content style="fixed-case">RAR</jats:styled-content>α in cancer cells. <jats:styled-content style="fixed-case">SUMO</jats:styled-content>‐1 modification may thus serve an important role in controlling <jats:styled-content style="fixed-case">ATRA</jats:styled-content>‐induced cell differentiation in cancers.</jats:p></jats:sec><jats:sec><jats:title>Structured digital abstract</jats:title><jats:p>
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<jats:list-item><jats:p><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.uniprot.org/uniprot/P63165">SUMO1</jats:ext-link>
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.ebi.ac.uk/ontology-lookup/?termId=MI:0915">physically interacts</jats:ext-link> with <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.uniprot.org/uniprot/P10276">RAR-alpha</jats:ext-link> by <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.ebi.ac.uk/ontology-lookup/?termId=MI:0006">anti bait coip</jats:ext-link> (<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.ebi.ac.uk/intact/interaction/EBI-9520843">1</jats:ext-link>, <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.ebi.ac.uk/intact/interaction/EBI-9520836">2</jats:ext-link>, <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.ebi.ac.uk/intact/interaction/EBI-9521217">3</jats:ext-link>)</jats:p></jats:list-item>
<jats:list-item><jats:p><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.uniprot.org/uniprot/P63165">SUMO-1</jats:ext-link><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.ebi.ac.uk/ontology-lookup/?termId=MI:0915">physically interacts</jats:ext-link> with <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.uniprot.org/uniprot/P10276">RAR-alpha</jats:ext-link> by <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.ebi.ac.uk/ontology-lookup/?termId=MI:0007">anti tag coimmunoprecipitation</jats:ext-link> (<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.ebi.ac.uk/intact/interaction/EBI-9521201">View interaction</jats:ext-link>)</jats:p></jats:list-item>
<jats:list-item><jats:p><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.uniprot.org/uniprot/P63165">SUMO1</jats:ext-link> and <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.uniprot.org/uniprot/P10276">RAR-alpha</jats:ext-link><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.ebi.ac.uk/ontology-lookup/?termId=MI:0403">colocalize</jats:ext-link> by <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.ebi.ac.uk/ontology-lookup/?termId=MI:0416">fluorescence microscopy</jats:ext-link> (<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="http://www.ebi.ac.uk/intact/interaction/EBI-9520823">View interaction</jats:ext-link>)</jats:p></jats:list-item>
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作者：
Zhang, Ligang;Masset, Patrick J.;Cao, Fuyong;Meng, Fangui;Liu, Libin*;...
期刊：
Journal of Alloys and Compounds,2011年509(9):3822-3831 ISSN：0925-8388
通讯作者：
Liu, Libin
作者机构：
[Cao, Fuyong; Jin, Zhanpeng; Liu, Libin; Zhang, Ligang] Cent S Univ, Sch Mat Sci & Engn, Changsha 410083, Hunan, Peoples R China.;[Masset, Patrick J.; Zhang, Ligang] Freiberg Univ Min & Technol, Ctr Innovat Competence Virtuhcon, D-09596 Freiberg, Germany.;[Jin, Zhanpeng; Liu, Libin; Zhang, Ligang] Cent S Univ, Sci Ctr Phase Diagram & Mat Design & Manufacture, Changsha 410083, Hunan, Peoples R China.;[Meng, Fangui] Cent S Univ Forestry & Technol, Sch Mat Sci & Engn, Changsha 410004, Hunan, Peoples R China.;[Liu, Libin] Cent S Univ, Sch Mat Sci & Engn, Lushan St 105, Changsha 410083, Hunan, Peoples R China.
通讯机构：
[Liu, Libin] C;Cent S Univ, Sch Mat Sci & Engn, Lushan St 105, Changsha 410083, Hunan, Peoples R China.
关键词：
Al-Cu-Er system;Annealed alloys;As cast alloy;As-cast;CALPHAD;CALPHAD method;Energy dispersive X ray spectroscopy;Phase relationships;Solidification simulation;Ternary phase;Alloys;Erbium;Phase diagrams;Phase equilibria;Scanning electron microscopy;Solidification;X ray diffraction;X ray diffraction analysis;X ray spectroscopy;Aluminum
摘要：
Cyclophilins (CyPs) are ubiquitous proteins and involved in protein folding and stress response due to peptidyl-prolyl cis-trans isomerase (PPIase) activity. In this study, a 975-base pair (bp) full-length complementary DNA (cDNA) gene, Camellia oleifera CyP (CoCyP, GenBank access no. FJ377540), was isolated from the conducted cDNA library of C. oleifera seeds. This cDNA encodes a polypeptide of 207 amino acids, which has an endoplasmic- reticulum-localized signal sequence at its N-terminus, but not the typical signal sequence at its C-terminus. Moreover, its putative amino acid sequence shares highest identity with those of Arabidopsis thaliana CyPB (84%) and Triticum aestivum CyPB (81%), and possesses a highly conserved central core domain including cyclosporin A (CsA)-binding sites, the tetrapeptide Ala-Ala-Pro-Ala-binding sites, residues required for PPIase catalysis and an insertion of seven amino acids of unknown function. Collectively, it is suggested that the CoCyP protein belongs to CyPB and has PPIase activity. An approximately 21 kDa protein was expressed via the recombinant pET-30b(+)/CoCyP in Escherichia coli. In this study, we tentatively put forward to the hypothesis that the gene expressing during the peak of seed lipid biosynthesis, might correlate with the caused harmful products in the lipid biosynthesis process, and protect cells against reactive oxygen species (ROS) damage; thus it may be crucial during lipid biosynthesis and stress responsiveness.
摘要：
Gametophytic self-incompatibility (GSI) is a genetically controlled mechanism to prevent inbreeding and promote out-crossing. Pyrus pyrifolia is a commercially important fruit tree that exhibits GSI. It is necessary to identify S-genotypes of cultivars for determination of cross-compatible combination prior to performing pear plantation and breeding programs. In this study, an important local cultivar 'Huobali' extensively used as parent in pear breeding programs, and its three progenies 'Mantianhong', 'Hongsucui' and 'Meirensu' were used for S-genotyping analysis by PCR-based molecular method. Agarose gel electrophoresis showed that two fragments were produced in each of the three progenies, while three in the 'Huobali'. After restriction digestion and sequencing analysis, the S-alleles in the four cultivars were determined. The allele of 345 bp from 'Huobali' was determined as a novel S-RNase allele that was tentatively denominated as S<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">44</sub>-RNase. RT-PCR revealed that the S<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">44</sub>-RNase was expressed specifically in the styles, which is consistent with the expression pattern of S-RNases. Comparison of genomic and cDNA sequences revealed an intron of 148 bp for the S<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">44</sub>-RNase. At the amino acid level, it shared 65% to 93% similarity with other Maloideae S-RNases. This study will be helpful in pear production and breeding programs.
会议名称：
2009 3rd International Conference on Bioinformatics and Biomedical Engineering
会议时间：
June 2009
会议地点：
Beijing, China
会议论文集名称：
2009 3rd International Conference on Bioinformatics and Biomedical Engineering
关键词：
Chinese white pear;gametophytic self-incompatibility;rapid amplification of cDNA ends;S-20-RNase;three-dimension (3D) structure
摘要：
To elucidate the function of S-allele and the possible molecular mechanism of gametophytic self-incompatibility (GSI) in Chinese white pear (Pyrus bretschneideri Rehd.), full length cDNA encoding S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">20</sub>
-RNase was cloned by rapid amplification of cDNA ends (RACE) approach from the cultivar 'Donghuang' (S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">8</sub>
S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">20</sub>
), and analyzed by bioinformatics method. S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">20</sub>
-RNase displayed typical sequence features of pear S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">20</sub>
-RNases, i.e. five conserved regions and a hypervariable (HV) region. The predicted three-dimension (3D) structure of the S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">20</sub>
-RNase was similar to that of 53-RNase of Japanese pear (P. pyrifolia), indicating that the S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">20</sub>
-RNase gene behaves as RNase rejecting pollen tube growth with the same S-haplotype as S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub>
-RNase does in GSI response. At the amino acid level, S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">20</sub>
-RNase showed 31.5% to 90.8% similarities with other rosaceous S-RNases. Phylogenetic analysis revealed that rosaceous S-RNases occurred before divergence of species, but after divergence of subfamilies Maloideae and Amygdaloideae.
会议名称：
2009 3rd International Conference on Bioinformatics and Biomedical Engineering
会议时间：
June 2009
会议地点：
Beijing, China
会议论文集名称：
2009 3rd International Conference on Bioinformatics and Biomedical Engineering
关键词：
Chinese sand pear;Gametophytic self-incompatibility;S-genotype;S-haplotype;SFBB gene
摘要：
Gametophytic self-incompatibility (GSI) is a genetically controlled mechanism to prevent inbreeding and promote out-crossing. It is controlled by a single multi-allelic S- locus which contains two separate genes: one stylar S-RNase gene and one pollen S-gene. In gametophytic self-incompatible Japanese pear (Pyrus pyrifolia Nakai.), SFBB-gamma genes have been identified as good candidates for pollen S-genes. To investigate sequence characters of the possible pollen S-gene in Chinese sand pear, degenerate primers PSFBG-F and PSFBG-R were designed for cloning the SFBB-gamma homologies in eight Chinese sand pear cultivars. By genomic PCR, sequencing and sequence analysis, a total of nine distinct sequences were identified which displayed the basic structural characterization of SFBISLF genes, i.e. an F-box motif and four variable regions. Of the nine sequences, five equaled to reported Japanese pear SFBB-gamma genes (S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub>
, S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub>
, S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sub>
, S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">5</sub>
and S
<sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">7</sub>
) and the other four were considered to be new ones which were named SFBB12-gamma, SFBB13-gamma, SFBBlS-gamma and SFBB22-gamma. The four new SFBB-gamma genes were expressed specifically in pollen grains. At the deduced amino acid level, they shared 20.4% to 97.7% similarities with other SFB/SLFs of rosaceous plants. The four SFBB-gamma genes cloned in this work will be useful for studying the interaction between SFBs and S-RNases, and clarifying the mechanism of GSI at the molecular level in Chinese pear.
摘要：
S-RNase encoded by gametophytically self-incompatible S-gene in plant pistil is an enzyme which causes pollen mRNA or rRNA of plants with identical genotypes to be degraded, resulting in self-incompatibility in plants. The fruiting percentage is less than 10% when cultivars with the same S-gene pollinate each other, but it reaches 60-100% in cultivars with different S-gene. China is rich in species or cultivars in pears (Pyrus spp.), and there are more than 500 cultivars only in Pyrus pyrifolia. However, the genetic resources and S genotypes are not yet clear in those cultivars. In this paper, 48 cultivars of P. pyrifolia in China were studied by PCR-RFLP with specific primers of S-gene, i.e., 'FTQQYQ' and 'anti-IIWPNV'. However, by this method, seven fragments in five cultivars, i.e., 'Huanghua', 'Guiguan', 'Changxixue', 'Maogong' and 'Taihuang' pears, could not be identified. These fragments were reclaimed, sequenced and compared with the data of GenBank. Seven new alleles of S-RNase were obtained from those cultivars, and parts of their sequences were registered in GenBank. They are S11-RNase, S12-RNase, S 13-RNase, S14-RNase, S15-RNase, S 16-RNase and S25-RNase, respectively. The partial sequences of the seven genes are highly homological with that of S 1-10-RNase gene, and the homology of hyper variable region (HV) of the seven alleles is less than 80%, demonstrating that HV region is the specific segment in which S-RNase recognizes pollen. The results from experiments of controlled cross in different cultivars also corroborate the validity of aforementioned facts.
