作者： Yang, Xue-Mei;Hui, Yu;Zhao, Lv-Quan;Zhu, Dao-Hong;Zeng, Yang;...

期刊： Insects,2021年12(11) ISSN：2075-4450

通讯作者： Yang, Xiao-Hui

作者机构： [Yang, Xue-Mei; Yang, Xiao-Hui; Hui, Yu] Hunan Normal Univ, Key Lab Prot Chem & Dev Biol Fish, Educ Minist China, State Key Lab Dev Biol Freshwater Fish,Coll Life, Changsha 410081, Peoples R China.;[Zhao, Lv-Quan] Nanjing Forestry Univ, Coll Forestry, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China.;[Zhu, Dao-Hong; Zeng, Yang] Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Peoples R China.

通讯机构： [Yang, Xiao-Hui] H;Hunan Normal Univ, Key Lab Prot Chem & Dev Biol Fish, Educ Minist China, State Key Lab Dev Biol Freshwater Fish,Coll Life, Changsha 410081, Peoples R China.

关键词： Auxin;Bacterial community;Cytokinin;Gall wasp;Insect galls;Lithocarpus glaber;Lithosaphonecrus arcoverti-cus

摘要： Simple Summary:& nbsp;Insect galls are characterized by high concentrations of auxins and cytokinins. We calculated the correlation between the concentrations of indoleacetic acid (IAA), trans-zeatin riboside (tZR) and isopentenyladenine (iP) and the bacterial community structure of Lithosaphonecrus arcoverticus galls. Our results indicated the concentrations of IAA, tZR and iP were positively correlated with the bacterial community structure of L. arcoverticus galls. We suggest the high concentrations of IAA, tZR and iP may affect the bacterial community structure of L. arcoverticus galls.& nbsp;Insect galls are the abnormal growth of plant tissues induced by a wide variety of galling insects and characterized by high concentrations of auxins and cytokinins. It remains unclear whether the auxins and cytokinins affect the bacterial community structure of insect galls. We determined the concentrations of indoleacetic acid (IAA) as an example of auxin, trans-zeatin riboside (tZR) and isopentenyladenine (iP) as cytokinins in Lithosaphonecrus arcoverticus (Hymenoptera: Cynipidae) galls and the galled twigs of Lithocarpus glaber (Fagaceae) using liquid chromatography-tandem mass spectrometry. Moreover, for the first time, we compared the bacterial community structure of L. arcoverticus galls and galled twigs by high-throughput sequencing, and calculated the Spearman correlation and associated degree of significance between the IAA, tZR and iP concentrations and the bacterial community structure. Our results indicated the concentrations of IAA, tZR and iP were higher in L. arcoverticus galls than in galled twigs, and positively correlated with the bacterial community structure of L. arcoverticus galls. We suggest the high concentrations of IAA, tZR and iP may affect the bacterial community structure of L. arcoverticus galls.

语种： 英文

作者： Hou, Han-Qing;Zhao, Gao-Zhi;Su, Cheng-Yuan;Zhu, Dao-Hong*

期刊： Entomologia Experimentalis et Applicata,2020年168(10):752-765 ISSN：0013-8703

通讯作者： Zhu, Dao-Hong

作者机构： [Hou, Han-Qing; Zhao, Gao-Zhi; Zhu, Dao-Hong; Su, Cheng-Yuan] Cent South Univ Forestry& Technol CSUFT, Coll Life Sci & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zhu, Dao-Hong] C;Cent South Univ Forestry& Technol CSUFT, Coll Life Sci & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

关键词： parasitoid;Wolbachia;MLST;horizontal transmission;recombination;multiple infections;Oriental chestnut gall wasp;Hymenoptera;Cynipidae;Fagaceae;Castanea

摘要： The Oriental chestnut gall wasp, Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae), is a global invasive pest that causes serious damage to almost all chestnut species belonging to the Castanea genus (Fagaceae). Dryocosmus zhuili Liu et Zhu is a recently described sibling species of D. kuriphilus, which induces galls on Castanea henryi (Skan) Rehd. et Wils. There are many indigenous parasitoid species in China which play an important role in the natural regulation of their population dynamics. Wolbachia is a maternally inherited α-proteobacterium widely found in arthropods. This study screened for the presence of Wolbachia in the two chestnut gall wasps and in six parasitoid species from 12 populations, to investigate the prevalence patterns of Wolbachia in the chestnut gall wasp-parasitoid communities. We found that D. zhuili and four parasitoid species were infected with Wolbachia; among them, all individuals of the two populations of Megastigmus sp. had multiple Wolbachia infections. By using multilocus sequence types to characterize bacterial strains, three new sequence types were identified. The Wolbachia strains infecting D. zhuili (ST-507), Torymus sinensis Kamijo (ST-508), and Sycophila variegata (Curtis) (ST-508) belonged to supergroup A, whereas the Wolbachia strain infecting Megastigmus nipponicus Kamijo (ST-503) belonged to supergroup B. Our results also suggested that horizontal transmission of Wolbachia occurs between chestnut gall wasps and their parasitoids. Moreover, multiple Wolbachia infections of Megastigmus sp. may be due to gene recombination and horizontal transmission. © 2020 The Netherlands Entomological Society

语种： 英文

作者： Xie, Yi-Fei;Wang, Xiu-Dan;Zhong, Wu-Hong;Zhu, Dao-Hong*;He, Zhen*

期刊： Frontiers in Physiology,2020年11:532176 ISSN：1664-042X

通讯作者： He, Zhen;Zhu, Dao-Hong

作者机构： [Xie, Yi-Fei; Zhong, Wu-Hong; He, Zhen] Hunan Acad Forestry, Changsha, Peoples R China.;[Xie, Yi-Fei; Zhu, Dao-Hong; Wang, Xiu-Dan] Cent South Univ Forestry & Technol, Coll Life Sci, Changsha, Peoples R China.

通讯机构： [He, Zhen] H;[Zhu, Dao-Hong] C;Hunan Acad Forestry, Changsha, Peoples R China.;Cent South Univ Forestry & Technol, Coll Life Sci, Changsha, Peoples R China.

关键词： differentially expressed gene;entomopathogenic nematode;heat shock response;Steinernema carpocapsae;transcriptome

摘要： The entomopathogenic nematode Steinernema carpocapsae has been used for control of soil insects. However, S. carpocapse is sensitive to environmental factors, particularly temperature. We studied an S. carpocapse group that was shocked with high temperature. We also studied the transcriptome-level responses associated with temperature stress using a BGIseq sequencing platform. We de novo assembled the reads from the treatment and control groups into one transcriptome consisting of 43.9 and 42.9 million clean reads, respectively. Based on the genome database, we aligned the clean reads to the Nr, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases and analyzed the differentially expressed genes (DEGs). Compared with the control, the heat-shocked group had significant differential expression of the heat shock protein (HSP) family, antioxidase [glutathione S-transferases (GSTs) and superoxide dismutase (SOD)], monooxygenase (P450), and transcription factor genes (DAF-16 and DAF-2). These DEGs were demonstrated to be part of the Longevity pathway and insulin/insulin-like signaling pathway. The results revealed the potential mechanisms, at the transcriptional level, of S. carpocapsae under thermal stress. © Copyright © 2020 Xie, Wang, Zhong, Zhu and He.

语种： 英文

作者： Yang, Xiao-Hui*;Li, Xiang-Mei;Zhu, Dao-Hong

期刊： ECOLOGICAL ENTOMOLOGY,2020年45(5):945-954 ISSN：0307-6946

通讯作者： Yang, Xiao-Hui

作者机构： [Yang, Xiao-Hui; Li, Xiang-Mei] Hunan Normal Univ, Life Sci, Changsha, Peoples R China.;[Zhu, Dao-Hong] Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha, Peoples R China.;[Yang, Xiao-Hui] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Peoples R China.

通讯机构： [Yang, Xiao-Hui] H;Hunan Normal Univ, Coll Life Sci, Changsha 410081, Peoples R China.

关键词： insect gall;nutrition hypothesis;amino acid;Andricus mukaigawae

摘要： 1. Insect galls are abnormal plant growths that develop in response to a stimulus provided by a galling insect. The nutrition hypothesis suggests that the concentrations of nutritive compounds in galls are changed to provide optimum nutrition for the larvae and adults of galling insects. 2. To test the nutrition hypothesis, we determined the concentrations of 20 free amino acids in galls and in galled and ungalled twigs of Quercus fabri during the larval and adult stages of Andricus mukaigawae using high-performance liquid chromatography with ultraviolet–visible detection. 3. At the larval stage, the concentrations of 12 out of 20 amino acids in A. mukaigawae galls were significantly higher than those in galled and ungalled twigs. Asparagine and tryptophan were the most abundant nonessential and essential amino acids, respectively, in A. mukaigawae galls. 4. At the adult stage, the concentrations of most amino acids, except proline, were significantly lower in A. mukaigawae galls than in galled and ungalled twigs. The A. mukaigawae adults may not manipulate amino acid levels because the adults do not feed on galls. The decrease of amino acid levels in adult galls may be viewed as a depletion. 5. The composition of free amino acids in A. mukaigawae galls was significantly different from the composition in galled and ungalled twigs in both the larval and adult stages. 6. Our results may support the nutrition hypothesis. We suggest that a high concentration of proline in A. mukaigawae galls may protect larvae and adults from plant defense responses. © 2020 The Royal Entomological Society

语种： 英文

作者： Zeng, Yang;Zhou, Feng-Hao;Zhu, Dao-Hong*

期刊： Scientific Reports,2018年8(1):9695 ISSN：2045-2322

通讯作者： Zhu, Dao-Hong

作者机构： [Zhou, Feng-Hao; Zhu, Dao-Hong; Zeng, Yang] Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zhu, Dao-Hong] C;Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

摘要： Sexual selection allows male individuals to adopt different evolutionary strategies in mating system. In this study, we determined whether dominance affected reproductive fitness of male crickets Velarifictorus aspersus during both pre-copulatory and post-copulatory selection when we excluded male-male competition. The results showed that females mated more often with male winners only during the first 2 h after a fight when male winners were more likely to produce courtship songs than losers. However, females did not retain the attached spermatophores of male winners longer than those of male losers, and the fecundity and fertilization success also did not differ significantly between females mated different times with male winners and losers. Instead, the fertilization success was positively correlated with male body weight. These results suggest that a recent wining experience increases reproductive fitness of males during pre-copulatory selection, but females may prefer larger males rather than winners during post-copulatory selection. The incoordination between pre- and post-copulatory selection may allow males to adopt different evolutionary strategies in mating system.

语种： 英文

作者： Zhao, Lv-Quan;Chai, Huai-lin;Zhu, Dao-Hong*

期刊： Evolutionary Biology,2017年44(1):91-99 ISSN：0071-3260

通讯作者： Zhu, Dao-Hong

作者机构： [Zhu, Dao-Hong; Chai, Huai-lin; Zhao, Lv-Quan] Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha, Hunan, Peoples R China.;[Zhao, Lv-Quan] Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Coll Forestry, Nanjing, Jiangsu, Peoples R China.

通讯机构： [Zhu, Dao-Hong] C;Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha, Hunan, Peoples R China.

关键词： Velarifictorus ornatus;Wing polymorphism;Males;Trade-off;Fertilization success

摘要： A trade-off between flight capability and reproduction is well known in adult females of the wing-dimorphic cricket Velarifictorus ornatus, but it is not clear whether such a trade-off exists in adult males of the species. In the present study, we investigated sexual maturation time, mating frequency, and the fertilization success of spermatophores after sequential mating in long-winged (LW) and short-winged (SW) adult males of V. ornatus to evaluate the potential reproductive advantage of the SW over the LW male morph. We found that the SW males of V. ornatus attained sexual maturity earlier and produced heavier spermatophores during the early stage after adult emergence than their LW counterparts. Additionally, within a 24-h mating period, the SW males showed a higher mating frequency, greater spermatophore weight, and shorter intermating time interval compared with their LW counterparts. Although females copulated with the two male morphs produced eggs of similar size, fertilization success by SW males was significantly higher than by the LW males. These results provide support for a trade-off between dispersal capability and reproduction success in wing-dimorphic males of V. ornatus. © 2016, Springer Science+Business Media New York.

语种： 英文

作者： Zheng, Yan;Zhou, Feng-Hao;Kang, Wei-Nan;Zhu, Dao-Hong*

期刊： ECOLOGICAL ENTOMOLOGY,2017年42(4):500-505 ISSN：0307-6946

通讯作者： Zhu, Dao-Hong

作者机构： [Zheng, Yan; Zhou, Feng-Hao; Kang, Wei-Nan; Zhu, Dao-Hong] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zhu, Dao-Hong] C;Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

关键词： Cricket;crowding;development;feeding behaviour;survivorship;unfertilised eggs

摘要： 1. Female insects of many species lay both fertilised and unfertilised eggs, with the latter accounting for up to 50% of the total number laid. These unfertilised eggs do not hatch and so their relevance is unclear. 2. In the present study, it was found that nymphs of the cricket Velarifictorus aspersus ate unfertilised eggs regardless of whether there was other food available. This provision enabled nymphs to develop successfully when no other food was provided, and to gain additional body mass when other food was available under crowded conditions. These results suggest that unfertilised eggs have an important role in the survival and development of nymphs. 3. Nymphs preferred to eat unfertilised rather than fertilised eggs, suggesting that they may have the ability to distinguish between these two types of eggs. 4. Crowding promoted feeding on unfertilised eggs and accelerate nymphal development, suggesting that greater food consumption accounts for faster growth under grouped conditions. © 2017 The Royal Entomological Society

语种： 英文

作者： 杨筱慧;李香妹;朱道弘

期刊： 华中昆虫研究,2017年(00):254-254

作者机构： 湖南师范大学生命科学学院,长沙410081;中南林业科技大学生命科学学院,长沙410004

会议名称： 华中三省昆虫学会2017年学术年会

会议时间： 2017-11-03

会议地点： 河南信阳

会议论文集名称： 华中三省昆虫学会2017年学术年会论文集

关键词： 虫瘿;营养假说;氨基酸;向川安瘿蜂

摘要： 虫瘿是植物响应致瘿昆虫刺激而异常生长形成的非正常组织。营养假说认为致瘿昆虫诱导虫瘿营养物质的含量高于植物未成瘿组织以满足自身营养需求。瘿蜂(膜翅目,瘿蜂科)幼虫生活于虫瘿的虫室内,以虫瘿为食,而成虫一般不取食,于虫瘿咬破一个羽化口而逃离。为验证营养假说,我们使用液相色谱首次测定了向川安瘿蜂无性世代幼虫和成虫期虫瘿、成瘿枝和未成瘿枝的20种游离氨基酸含量,包括10种非必需和10种必需氨基酸。结果表明,对于游离氨基酸总量,幼虫期虫瘿显著高于成瘿和未成瘿枝,成虫期则相反,且幼虫期虫瘿显著高于成虫期虫瘿。对于必需氨基酸总量,在幼虫和成虫期,虫瘿显著低于成瘿和未成瘿枝,且幼虫和成虫期虫瘿无显著差异。对于不同氨基酸含量,幼虫和成虫期虫瘿的脯氨酸含量均显著高于成瘿和未成瘿枝,但成虫期仅脯氨酸1种,而幼虫期虫瘿有12种氨基酸含量显著高于未成瘿枝和成瘿枝,其中,天冬酰胺和色氨酸分别是含量最高的非必需和必需氨基酸。对于氨基酸组分,主成分分析表明,在幼虫和成虫期,虫瘿的氨基酸组分均与成瘿和未成瘿枝不同,而成瘿和未成瘿枝的组分相似。笔者的研究结果支持营养假说,向川安瘿蜂无性世代的幼虫可能通过取食改变了虫瘿的游离氨基酸组分,诱导虫瘿的游离氨基酸总量及多数氨基酸含量,特别是与氮运输相关的天冬酰胺含量显著高于植物组织,这有利于满足幼虫的营养需求。成虫可能通过咬破虫瘿改变了虫瘿的游离氨基酸组分,但成虫期虫瘿游离氨基酸总量和多数氨基酸含量显著低于植物组织,这可能与成虫不取食虫瘿有关。需要指出的是,虫瘿较高含量的氨基酸不仅有利于向川安瘿蜂幼虫的营养需求,也可能有利于幼虫和成虫抵御植物的防御反应,如脯氨酸有利于清除植物防御反应产生的活性氧。

语种： 中文

作者： Zhao, Lv-Quan;Liao, Hong-Yu;Zeng, Yang;Wu, Hong-Jun;Zhu, Dao-Hong*

期刊： Entomologia Experimentalis et Applicata,2017年163(1):35-42 ISSN：0013-8703

通讯作者： Zhu, Dao-Hong

作者机构： [Zhao, Lv-Quan] Nanjing Forestry Univ, Coll Forestry, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Jiangsu, Peoples R China.;[Zhu, Dao-Hong; Wu, Hong-Jun; Liao, Hong-Yu; Zeng, Yang] Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zhu, Dao-Hong] C;Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

关键词： wing polymorphism;resource allocation;Orthoptera;Gryllidae;trade-off;food conversion;conversion efficiency;lipase;amylase;trypsin-like activity

摘要： Velarifictorus ornatus (Shiraki) (Orthoptera: Gryllidae) display distinct wing variation, and a physiological trade-off between reproduction and flight muscles has been observed in long-winged (LW) and short-winged (SW) females. To understand the physiological basis of this trade-off, we investigated the difference in the food digestion capability and digestive enzyme activity between LW and SW females. We found that the efficiency of conversion of digested food into body matter of SW females was significantly higher than that of the LW females, although there was no difference in the approximate digestibility between LW and SW females during the first 12 days after the adult emergence. Similarly, growth and relative growth were significantly higher in SW females. The food consumption of SW females was significantly higher than that of the LW females only in the first 6 days after the adult emergence, suggesting enhanced ovary development in SW females is probably because of the elevated efficiency of conversion of digested food into body matter coupled with greater consumption in the early stage after the adult emergence. Trypsin-like activity was higher in SW females than in LW females, whereas no differences in the fresh weight of the midguts (including content) were observed between LW and SW females at 1, 3, or 5 h after refeeding after starvation for 18 h. In contrast, amylase activity varied significantly depending on time lapse after refeeding, being significantly higher in LW females than in SW females at 1 h after refeeding, but significantly lower in LW females than in SW females at 3 and 5 h after refeeding. The lipase activity displayed a similar trend in both LW and SW females after refeeding, with an initial decline followed by a slight increase; and no difference was observed between LW and SW females at any stage after refeeding.

语种： 英文

作者： Zhao, Lv-Quan;Chai, Huai-Lin;Wu, Hong-Jun;Zhu, Dao-Hong*

期刊： EUROPEAN JOURNAL OF ENTOMOLOGY,2017年114:267-274 ISSN：1210-5759

通讯作者： Zhu, Dao-Hong

作者机构： [Zhao, Lv-Quan] Nanjing Forestry Univ, Coll Forestry, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing, Jiangsu, Peoples R China.;[Zhu, Dao-Hong; Wu, Hong-Jun; Chai, Huai-Lin] Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha, Hunan, Peoples R China.

通讯机构： [Zhu, Dao-Hong] C;Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha, Hunan, Peoples R China.

关键词： Gryllidae;Mating competition;Orthoptera;Sing song;Trade-off;Velarifictorus ornatus;Wing polymorphism

摘要： The trade-offbetween flight capability and reproduction is well known in adult males of insects with wing dimorphism but the reproductive advantage of short-winged (SW) males over long-winged (LW) males appears to vary across insect taxa. In the present study, we determined the difference in the mating ability of SW and LW males of Velarifictorus ornatus (Orthoptera: Gryllidae) in order to evaluate whether the SW male morph has a reproductive advantage. We found that the choice of a mate depended on the female. Compared with LW males, SW males had an obvious mating advantage when both SW and LW males courted females simultaneously, and that dealation significantly enhanced the mating ability of LW males. Losing the ability to produce songs reduced the mating advantage of SW males, thereby indicating that the greater mating advantage of SW males was related to the attractiveness of the song. In addition, the difference in the mating ability of LW and SW males was not related to body size or age. These results indicate that SW males of V. ornatus have a mating advantage over their LW counterparts because their underdeveloped flight muscles allow them to devote more resources to reproduction. © Institute of Entomology, Biology Centre, Czech Academy of Sciences, České Budějovice.

语种： 英文

作者： Zeng, Yang;Zhu, Dao-Hong*;Kang, Wei-Nan

期刊： Behavioral Ecology and Sociobiology,2016年70(3):429-435 ISSN：0340-5443

通讯作者： Zhu, Dao-Hong

作者机构： [Zhu, Dao-Hong; Kang, Wei-Nan; Zeng, Yang] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zhu, Dao-Hong] C;Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

关键词： Fighting;Resource;Cricket;Wing dimorphism;Life history trade-off

摘要： Because a trade-off between reproduction and dispersal has been commonly found in wing-dimorphic insects, males of different wing morphs may have a different demand on a mate or a territory. We hypothesized that males of wing-dimorphic insects may vary in fighting success over these two resources. To test this hypothesis, we compared morphological traits linked to fighting ability between long-winged (LW) and short-winged (SW) male Velarifictorus aspersus crickets (Gryllidae) and investigated their fighting success over a burrow or a mate. SW males gained more biomass than did LW males after molting to adulthood, indicating that morphological traits that can influence fighting ability differed between wing-dimorphic male crickets (Gryllidae). SW males won significantly more fights than did LW males when fighting for a mate. However, LW males were more aggressive than SW males when intruding the burrow of another male, and the winning probability of LW intruders was significantly greater than that of SW defenders. Fighting outcome significantly influenced the ownership of resources, and winning could help crickets (Gryllidae) become more successful in acquisition of the target resource. Our results suggested that trade-offs between life-history traits result in the differentiation of fighting strategies, such that both winged forms could succeed with their particular life-history strategy. © 2016, Springer-Verlag Berlin Heidelberg.

语种： 英文

作者： 朱锡勇;曾杨;朱道弘

期刊： 生命科学研究,2016年20(6):530-534 ISSN：1007-7847

作者机构： [曾杨; 朱道弘; 朱锡勇] 中南林业科技大学生命科学与技术学院,中国湖南长沙,410004

关键词： 丝带凤蝶;翅;尾突;红斑;季节多型

摘要： 丝带凤蝶(Sericinus montelus Gray)是一种具有较高观赏价值的昆虫,存在季节多型现象。为明确其季节多型的科学划分依据,利用自动计算机辅助设计软件对丝带凤蝶长沙种群野外成虫的翅面积、尾突长度及面积、翅红斑面积进行测量,分析各世代成虫上述特征的差异性。结果显示,春型成虫的翅面积、尾突长度和相对面积均显著低于夏型成虫,而其前翅红斑相对面积显著大于夏型成虫。另外,春型雄成虫的后翅反面总红斑及臀角区红斑的相对面积显著高于夏型雄成虫,但是雌成虫并无显著性差异。以上结果表明丝带凤蝶的季节多型现象存在性间差异;前、后翅面积,后翅尾突长度及相对面积,前翅红斑相对面积是区分丝带凤蝶不同季节型的主要指标;而后翅反面红斑相对面积及臀角区红斑相对面积则是区分雄成虫季节多型的重要指标。

语种： 中文

作者： Zeng, Yang;Zhu, Dao-Hong*

期刊： ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA,2015年108(6):1053-1059 ISSN：0013-8746

通讯作者： Zhu, Dao-Hong

作者机构： [Zhu, Dao-Hong; Zeng, Yang] Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zhu, Dao-Hong] C;Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

关键词： hormone manipulation;hemolymph;JH III titer;Velarifictorus aspersus;wing polymorphism

摘要： The classical model of juvenile hormone (JH)-mediated wing polymorphism was proposed in the early 1960s, and numerous studies have tested this model using exogenous hormone treatment. However, this indirect method may yield misleading results, necessitating direct quantification of JH titers in insects. In this study, the effects of JH application on wing development and comparisons of hemolymph JH titers between presumptive long-winged (LW) and short-winged (SW) nymphs in the cricket Velarifictorus aspersus (Walker) are investigated. The results show that treatment of presumptive LW nymphs with high-dose JH III significantly decreases the percentage of macropterous individuals during the penultimate and first half of the last stadia, indicating that exogenous JH III can suppress wing development in V. aspersus and that the last two stadia are critical periods for wing determination. However, application of precocene to presumptive SW nymphs does not induce macropterizing effects, and JH III titers are not significantly higher in presumptive SW females than in presumptive LW females during the critical period of wing development. Therefore, this model may not be applicable to this species and the brachypterizing effects associated with JH application may be induced by affecting other innate factors rather than by direct JH titers in hemolymph.

语种： 英文

作者： Zhu, Dao-Hong;Liu, Zhiwei*;Lu, Peng-Fei;Yang, Xiao-Hui;Su, Cheng-Yuan;...

期刊： Journal of Insect Science,2015年15(1) ISSN：0970-3837

通讯作者： Liu, Zhiwei

作者机构： [Lu, Peng-Fei; Zhu, Dao-Hong; Yang, Xiao-Hui; Su, Cheng-Yuan] Cent South Univ Forestry & Technol, Coll Life Sci, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.;[Liu, Zhiwei] Eastern Illinois Univ, Dept Biol Sci, Charleston, IL 61920 USA.;[Liu, Zhiwei] Field Museum Nat Hist, Integrat Res Ctr, Chicago, IL 60605 USA.;[Liu, Peter] Eastern Illinois Univ, Sch Technol, Charleston, IL 61920 USA.

通讯机构： [Liu, Zhiwei] E;Eastern Illinois Univ, Dept Biol Sci, Charleston, IL 61920 USA.

关键词： Dryocosmus zhuili;chestnut;gall wasp;new species;southeastern China

摘要： A new gall wasp species, Dryocosmus zhuili Liu et Zhu, is herein described from the southeastern Fujian province of China. The new species induces galls on trees of Henry's chestnut, Castanea henryi, which is also a native host for the notorious Oriental chestnut gall wasp (OCGW, Dryocosmus kuriphilus Yasumatsu). D. zhuili overlaps with OCGW in emergence time and induces galls morphologically similar to that of OCGW on similar plant parts. In a previous study, we reported considerable divergence between mtDNA CO1 (mitochondrial DNA Cytochrome c oxidase subunit I) sequences of these wasps and the true OCGW wasps and suggested the existence of a cryptic species. Herein, we confirm the identity of the new species based on morphological and biological differences and provide a formal description. Although the new species is relatively easily separated from OCGW on basis of morphology, field identification involving the two species can still be problematic because of their small body size, highly similar gall morphology, and other life history traits. We further discussed the potential of the new species to be a pest for the chestnut industry and the consequences of accidental introduction of this species into nonnative areas, especially with regard to the bisexual reproduction mode of the new species in contrast to the parthenogenetic reproduction mode of OCGW. © The Author 2015.

语种： 英文

作者： Zhao, Lu-Quan;Zhu, Dao-Hong*

期刊： Journal of Insect Science,2014年14(1):117 ISSN：0970-3837

通讯作者： Zhu, Dao-Hong

作者机构： [Zhao, Lu-Quan; Zhu, Dao-Hong] Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.;[Zhu, Dao-Hong] Hunan First Normal Univ, Zool Lab, Changsha 410205, Hunan, Peoples R China.

通讯机构： [Zhu, Dao-Hong] C;Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

关键词： Gryllidae;density;injury;photoperiod;wing dimorphism

摘要： The effects of environmental factors and appendage injury on the wing variation in Velarifictorus ornatus (Shiraki) (Orthoptera: Gryllidae) were investigated. The percentage of micropters was more than 95% when the nymphs were reared at constant photoperiods, and changing photoperiod did not affect wing variation in V. ornatus at 25 or 30°C. In the crowding experiment, the percentage of macropters was only 11.2% when the nymphs were reared separately at 25°C. In contrast, the percentage of macropters was significantly higher when the rearing density was increased to two nymphs per container and lower when the rearing density was increased to five or 10 nymphs per container. These results indicate that low and high rearing densities induce micropters, but intermediate rearing density stimulates the formation of macropters. Meanwhile, severance of appendages, such as antennae, femora, and tibiae, in the nymph stage exerted a micropterizing effect. The period sensitive to such stresses ranged from 35 to 60 days of nymph development.

语种： 英文

作者： Zeng, Yang;Zhu, Dao-Hong*;Zhao, Lu-Quan

期刊： Evolutionary Biology,2014年41(3):397-403 ISSN：0071-3260

通讯作者： Zhu, Dao-Hong

作者机构： [Zhao, Lu-Quan; Zhu, Dao-Hong; Zeng, Yang] Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zhu, Dao-Hong] C;Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

关键词： Migration;Reproductive development;Trade-off;Dealation;Food consumption

摘要： Flight dimorphism has been considered to result from a balance of costs and benefits between flight capability and reproduction. The validity of the hypothesis, however, has been challenged recently. In this study, we examined the effect of flight time on trade-off between flight capability and reproductive development in Velarifictorus aspersus and we found that flight of 5 min did not promote reproductive development of long-winged (LW) adults, but flight of 30, 60, or 120 min could promote reproductive development both in female and male crickets. The results indicate that flight time may serve as a signal for LW V. aspersus to switch from migration to reproduction, and trade-off between flight ability and reproduction may be attenuated when flight time reaches a critical threshold. In addition, rapid reproductive development occurred before dealation when LW insects were allowed to fly for 30 min, which indicates that flight may influence reproductive development directly. Food consumption of short-winged adults was significantly higher than that of unflown LW adults or LW adults with 5 min flight, but similar to that of LW adults with 30, 60, or 120 min flight, suggesting that difference of reproductive development may be positively correlated with their food consumption. © 2014 Springer Science+Business Media New York.

语种： 英文

作者： Zeng, Yang;Zhu, Dao-Hong*

期刊： ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA,2014年107(6):1066-1071 ISSN：0013-8746

通讯作者： Zhu, Dao-Hong

作者机构： [Zhu, Dao-Hong; Zeng, Yang] Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zhu, Dao-Hong] C;Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

关键词： Latitudinal trend;Photoperiodic response;Velarifictorus micado

摘要： We examined whether body size, development time, and wing dimorphism are affected by latitude in the cricket, Velarifictorus micado (Saussure). We found that the number of days required for nymphal development decreased with increasing latitude, suggesting that shorter periods of warm temperatures in the north resulted in faster development. Body size varied between populations, and followed the converse to Bergmann's rule, indicating that limited development time at higher latitudes resulted in smaller body size. Wing dimorphism in V. micado was influenced by both photoperiod and latitude. A higher proportion of macropters occurred under longer photoperiod conditions, suggesting that wing dimorphism in V. micado was the result of seasonal adaptation. The critical photoperiod was shorter for the southern populations than that for the northern populations, and the incidence of macroptery decreased with increasing latitude, suggesting that wing dimorphism in V. micado correlated with climatic variation. © 2014 Entomological Society of America.

语种： 英文

作者： Wu, H. J.;Zhu, D. H.*;Zeng, Y.;Zhao, L. Q.;Sun, G. X.

期刊： ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA,2014年107(1):113-118 ISSN：0013-8746

通讯作者： Zhu, D. H.

作者机构： [Zhao, L. Q.; Wu, H. J.; Zhu, D. H.; Zeng, Y.; Sun, G. X.] Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zhu, D. H.] C;Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

关键词： crowding;appendage damage;sensitive stage;wing dimorphism

摘要： In wing dimorphic cricket Velarifictorus micado Saussure, group rearing could induce macropters, but rearing density had a nonlinear effect on the incidence of macroptery. Frequency of macropters increased along with rearing density under low-density conditions, but it decreased gradually under high densities, especially for the females. This result indicates that flight ability is required to escape from crowded conditions, but there is a factor that would suppress wing development under high-population density in V. micado. Investigation of body injury showed that incidence of body injury increased along with rearing density, especially for the antenna. Removal of antennas or hindlegs in the penultimate nymphal stage exerted a strong micropterizing effect. These results suggest that brachypterizing effect of high density may be related with body injury. Removed antennas at different instars, brachypterizing effect was only significant in sixth instar (penultimate instar) for males, and sixth and seventh instar (last instar) for females, which suggests that penultimate and last instars may be sensitive stages in winglength development to the effect of body injury in V. micado © 2014 Entomological Society of America.

语种： 英文

作者： Zhu, Dao-Hong*;Zhao, Qin;Tanaka, Seiji

期刊： ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA,2013年106(1):66-71 ISSN：0013-8746

通讯作者： Zhu, Dao-Hong

作者机构： [Zhu, Dao-Hong; Zhao, Qin] Cent S Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.;[Zhu, Dao-Hong] Hunan First Normal Univ, Zool Lab, Changsha 410205, Hunan, Peoples R China.;[Tanaka, Seiji] Natl Inst Agrobiol Sci Ohwashi, Locust Res Lab, Tsukuba, Ibaraki 3058634, Japan.

通讯机构： [Zhu, Dao-Hong] C;Cent S Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha 410004, Hunan, Peoples R China.

关键词： accessory gland;mating behavior;preovipositional period;fecundity;hatchability

摘要： Mating not only enables the female to acquire spermatozoa from the male, but may also enhance female fecundity and induce egg laying in many insect species. The general consensus is that the increased fecundity that results from mating is because of substances obtained from the male reproductive accessory gland at the time of mating. To understand better the effects of mating on reproduction, we compared the longevity, fecundity, and hatchability of eggs laid by females of a tropical strain of Locusta migratoria Linné that had been subject to different mating treatments. Females that were paired with males from which the testes, accessory glands, testes and accessory glands, or genitalia were extirpated did not differ significantly from females mated with intact males in some reproductive parameters. Their reproductive rates up to 92%, preovipositional periods were around 24 d and ovipositional intervals were around 5 d. They laid ≈6 egg pods during their adult lives and produced ≈50 eggs per egg pod. However, isolated females or females physically separated from a male in the same cage only 41.2 or 48.0% laid eggs, over 80 d in preovipositional periods and nearly 8 d in ovipositional interval; they only laid 1.3 or 1.1 egg pods throughout its life span and produced ≈30 eggs per egg pod, which were significantly different with the value for females mated with operated and intact males. The hatchability of eggs laid by females paired with the surgically altered males (around 20%) were significantly lower than those laid by females mated with intact males (83.2% in mean), but significantly higher than those laid by parthenogenic females or females physically separated from a male in the same cage (3.2 or 4.4%, respectively). The results suggested that, in addition to the transfer of spermatozoa and accessory gland secretions, physical contact between males and females, especially mounting behavior, might also promote female oviposition, increasing fecundity and egg hatchability. © 2013 Entomological Society of America. © 2013 Entomological Society of America.

语种： 英文

作者： Yang, Xiao-Hui;Zhu, Dao-Hong*;Liu, Zhiwei;Zhao, Ling;Su, Cheng-Yuan

期刊： PLOS ONE,2013年8(11):e78970 ISSN：1932-6203

通讯作者： Zhu, Dao-Hong

作者机构： [Zhao, Ling; Yang, Xiao-Hui; Zhu, Dao-Hong; Su, Cheng-Yuan] Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha, Hunan, Peoples R China.;[Liu, Zhiwei] Eastern Illinois Univ, Dept Biol Sci, Charleston, IL 61920 USA.

通讯机构： [Zhu, Dao-Hong] C;Cent South Univ Forestry & Technol, Lab Insect Behav & Evolutionary Ecol, Changsha, Hunan, Peoples R China.

关键词： Wolbachia;Parasitic diseases;Burrowing;Insects;Larvae;Polymerase chain reaction;Phylogenetic analysis;Nematode infections

摘要： Wolbachia are maternally inherited endosymbiotic bacteria of arthropods and nematodes. In arthropods, they manipulate the reproduction of their hosts to facilitate their own spread in host populations, causing cytoplasmic incompatibility, parthenogenesis induction, feminization of genetic males and male-killing. In this study, we investigated Wolbachia infection and studied wsp (Wolbachia surface protein) sequences in three wasp species associated with the unisexual galls of A. mukaigawae with the aim of determining the transmission mode and the reason for multiple infections of Wolbachia. Frequency of Wolbachia infected populations for A. mukaigawae, Synergus japonicus (inquiline), and Torymus sp. (parasitoid) was 75%, 100%, and 100%, respectively. Multiple Wolbachia infections were detected in A. mukaigawae and S. japonicus, with 5 and 8 Wolbachia strains, respectively. The two host species shared 5 Wolbachia strains and were infected by identical strains in several locations, indicating horizontal transmission of Wolbachia. The transmission potentially takes place through gall tissues, which the larvae of both wasps feed on. Furthermore, three recombination events of Wolbachia were observed: the strains W8, W2 and W6 apparently have derived from W3 and W5a, W6 and W7, W4 and W9, respectively. W8 and W2 and their respective parental strains were detected in S. japonicus. W6 was detected with only one parent (W4) in S. japonicus; W9 was detected in Torymus sp., suggesting horizontal transmission between hosts and parasitoids. In conclusion, our research supports earlier studies that horizontal transmission of Wolbachia, a symbiont of the Rickettsiales order, may be plant-mediated or take place between hosts and parasitoids. Our research provides novel molecular evidence for multiple recombination events of Wolbachia in gall wasp communities. We suggest that genomic recombination and potential plant-mediated horizontal transmission may be attributable to the high levels of multiple Wolbachia infections observed in A. mukaigawae and S. japonicus.

语种： 英文