作者： Gao, Xiaolei;Yang, Ying;Ye, Jiawei;Xiong, Huan;Yuan, Deyi;...

期刊： Agronomy,2024年14(3):585- ISSN：2073-4395

通讯作者： Zou, F

作者机构： [Gao, Xiaolei; Xiong, Huan; Yang, Ying; Ye, Jiawei; Zou, Feng; Yuan, Deyi] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Peoples R China.;[Gao, Xiaolei; Xiong, Huan; Yang, Ying; Ye, Jiawei; Zou, Feng; Yuan, Deyi] Cent South Univ Forestry & Technol, Hunan Key Lab Coll & Univ Oil Tea Breeding, Changsha 410004, Peoples R China.

通讯机构： [Zou, F ] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Hunan Key Lab Coll & Univ Oil Tea Breeding, Changsha 410004, Peoples R China.

关键词： cytoplasmic male sterility;petaloid anther;calcium homeostasis;ROS metabolism;tapetal PCD;gene expression;Camellia oleifera

摘要： Cytoplasmic male sterility (CMS) plays a crucial role in the utilization of heterosis. The petaloid anther abortion in oil tea (Camellia oleifera Abel.) constitutes a CMS phenomenon, which is of great value for the hybrid breeding of oil tea. However, as the mechanism of its CMS is still poorly understood, it is necessary to study the cytology and physiological characteristics of anther abortion. In this study, a C. oleifera cultivar, Huashuo (HS), and its petalized CMS mutant (HSP) were used as materials to explore this mechanism. Compared with HS, cytological analysis demonstrated that HSP showed early-onset tapetum programmed cell death (PCD) and an organelle disorder phenotype during the tetrad stage. In HSP, anthers exhibited elevated levels of calcium deposition in anther wall tissues, tapetum layers, and microspores, and yet calcium accumulation was abnormal at the later stage. The contents of hydrogen peroxide and MDA in HSP anthers were higher, and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were lower than those of HS, which resulted in an excessive accumulation of reactive oxygen species (ROS). Real-time quantitative PCR confirmed that the transcription levels of CoPOD and CoCAT genes encoding key antioxidant enzymes in HSP were downregulated compared with HS in early pollen development; the gene CoCPK, which encodes a calcium-dependent protein kinase associated with antioxidase, was upregulated during the critical period. Thus, we suggest that excessive ROS as a signal breaks the balance of the antioxidant system, and along with an abnormal distribution of calcium ions, leads to the early initiation of PCD in the tapetum, and ultimately leads to pollen abortion for HSP. These results lay a cytological and physiological foundation for further studies on the CMS mechanism, and provide information for breeding male-sterile lines of C. oleifera.

语种： 英文

作者： Zhou, Xuan;Wang, Lu;Yin, Qian;Liu, Xinghui;Masabni, Joseph;...

期刊： Forests,2023年14(10):2057- ISSN：1999-4907

通讯作者： Zou, F

作者机构： [Xiong, Huan; Yin, Qian; Liu, Xinghui; Wang, Lu; Zou, Feng; Yuan, Deyi; Zhou, Xuan] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Peoples R China.;[Masabni, Joseph] Texas A&M Agri Life Res & Extens Ctr, 17360 Coit Rd, Dallas, TX 75252 USA.

通讯机构： [Zou, F ] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Peoples R China.

关键词： Castanea mollissima;floral morphology;phytohormone;RAN-seq;flower differentiation

摘要： Castanea mollissima is an important monoecious fruit crop with high economic and ecological value in China. However, its yield is restricted by an imbalanced ratio of male and female flowers for chestnut production. To address this issue, we examined the morphology of bisexual flower organs, measured the levels of endogenous hormones in the flowers, profiled gene expression related to plant hormone biosynthesis and signaling pathways and transcription factors, and investigated the effects of exogenous jasmonic acid (JA) and zeatin (ZT) hormone application on flower development in C. mollissima ‘Tanqiao’. Morphological studies indicated that the development of male and female flowers can be divided into nine and eight stages, respectively. Male flowers contained higher levels of gibberellic acid (GA3) and abscisic acid (ABA) than female flowers, whereas female flowers had higher levels of JA and ZT. The analysis of the Kyoto encyclopedia of genes and genomes (KEGG) pathways revealed that the major significant enrichment pathways of differentially expressed genes (DEGs) consisted of plant hormone signal transduction and zeatin biosynthesis. Through time-series analyses, we screened 3 genes related to jasmonic acid biosynthesis and signal transduction and 21 genes related to zeatin biosynthesis and transduction. Among these genes, only the gene family LOG, related to zeatin biosynthesis, was highly expressed in female flowers. This result indicated that LOG may be the core gene hormone family involved in regulating female flower development. However, a weighted gene co-expression network analysis (WGCNA) suggested that IDD7 was the core gene involved in regulating female flower development. The results of exogenous hormone application indicated that zeatin could greatly increase the quantity of fertile female flowers, but JA was not significant. These findings demonstrated that zeatin and transcription factors were crucial regulators in the formation of female flowers in C. mollissima.

语种： 英文

作者： 周璇;周乃富;杨莹;熊欢;袁德义;...

期刊： 中南林业科技大学学报,2023年43(12):56-66 ISSN：1673-923X

作者机构： 中南林业科技大学 经济林培育与保护教育部重点实验室,湖南 长沙 410004;[周乃富] 湖南省水资源研究和利用合作中心,湖南 长沙 410031;[ZOU Feng; 袁德义; 周璇; 杨莹; 熊欢] 中南林业科技大学

关键词： 板栗;雌花;雄花;转录组测序;差异表达基因(DEGs)

摘要： [目的]板栗Castanea mollisima为我国重要的木本粮食树种,其果实营养价值极高.花发育是板栗产量形成的重要基础.因此,研究板栗雌雄花不同发育时期的基因表达变化,挖掘板栗花发育关键基因,对进一步完善成花机理具有重要的意义.[方法]以不同时期'檀桥'板栗雌雄花为材料,采用石蜡切片技术观察雌雄花发育进程,利用转录组测序、生物信息学分析以及荧光定量PCR等方法,筛选'檀桥'板栗雌雄花发育的相关差异基因.[结果]细胞学观察雌花为雌蕊原基分化期和开放期,雄花为花药发育期和开放期.转录组测序共得到 90.16 Gb Clean Data,雌花不同发育时期共获得 1 094 个DEGs,其中 600 个上调,494 个下调;雄花不同发育时期共获得 3 407 个DEGs,其中 1 847 个上调,1 560 个下调;韦恩图显示两组间共有 663 个DEGs.雌花有 3 260 条DEGs注释到GO数据库中,分为细胞组分、分子功能及生物学过程 3 大类 42 条通路;雄花注释到 10 196 条DEGs分为 3 大类 47 条通路.KEGG富集分析显示雌雄花两组共有的差异基因显著富集在植物昼夜节律计划、淀粉和蔗糖代谢、类胡萝卜素生物合成代谢通路上;共筛选出 69 个植物昼夜节律计划、淀粉和蔗糖代谢以及激素信号转导相关差异表达基因.此外,WGCNA分析得到了 2 个hub基因BAM1、SAMT.[结论]光周期以及糖类化合物、激素信号转导相关基因PIF3、BAM1、SAUR等可能是'檀桥'板栗成花关键基因.这些结果为深入研究'檀桥'板栗花发育的分子机理奠定了理论基础.

语种： 中文

作者： 戴伟红;邹锋;江盈;左荣花;田诗义;...

期刊： 江西农业大学学报,2023年45(02):311-321 ISSN：1000-2286

作者机构： [戴伟红; 邹锋; 江盈; 左荣花; 田诗义; 殷慧敏; 熊欢] 中南林业科技大学林学院/经济林培育与保护教育部重点实验室

关键词： 板栗;幼苗;外生菌根真菌;光合作用;养分含量

摘要： 【目的】研究接种4种外生菌根真菌对‘檀桥’板栗（Castanea mollissima ‘Tanqiao’）幼苗生长的影响，为板栗菌根化育苗技术提供科学依据。【方法】以‘檀桥’板栗家系苗为材料，采用经课题组前期分离纯化和分子鉴定所获得的中华豆马勃（Pisolithus orientalis LY-8）、橙黄硬皮马勃（Scleroderma citrinum LY-9）、硬皮马勃属未知种1（Scleroderma sp. 1,LY-17-2）和未知种2（Scleroderma sp. 2,MB）进行接种，比较了接种和未接种‘檀桥’板栗幼苗的菌根侵染率、生长指标（苗高、地径、生物量、根系）、光合指标及氮磷钾养分含量的差异。【结果】（1）接种4个月后，4种外生菌根真菌均能成功侵染‘檀桥’板栗，接种LY-17-2的侵染率显著高于其他3种真菌，达86.40%。（2）接种4种菌根真菌均能显著提高‘檀桥’板栗幼苗苗高、地径、生物量、根系构型指标和苗木质量指数（SQI）。其中，接种LY-17-2的总生物量显著高于LY-9，达12.61 g/株；接种LY-8的幼苗总根长、表面积、体积、根尖数显著高于接种其他3种真菌的幼苗；接种LY-17-2和LY-8的SQI值（1.44和1.43）显著高于未接种的（0.76）。（3）接种LY-17-2的幼苗净光合速率和水分利用率最大，分别是6.51μmol/（m~2·s）和9.47μmol/mmol；接种LY-8的幼苗叶片叶绿素总含量显著高于接种其他3种真菌；接种4种菌根真菌均显著促进了‘檀桥’板栗幼苗在根、茎、叶中N、P含量的积累。【结论】接种4种外生菌根真菌均能扩大幼苗根系，促进植株对养分的积累，提高光合利用率，促进‘檀桥’板栗幼苗的生长。经综合比较，接种LY-8和LY-17-2对‘檀桥’板栗幼苗促生效果最好。

语种： 中文

作者： 陈雅;袁德义;李葵;杨颖慧;金亦佳;...

期刊： 江西农业大学学报,2020年42(1):118-126 ISSN：1000-2286

作者机构： 中南林业科技大学林学院;[邹锋; 袁德义; 林铭浩; 杨颖慧; 金亦佳; 陈雅] 经济林育种与栽培国家林业局重点实验室;湖南省怀化市林业科学研究所;[李葵] 怀化市林业科学研究所

关键词： 油茶;花粉活力;FDA荧光染色法;贮藏条件

摘要： 【目的】为筛选“三华系列”(‘华硕’、‘华金’、‘华鑫’)花粉活力适宜的检测方法及贮藏条件。【方法】研究采用FDA荧光染色法,I_2-KI法以及离体萌发法研究不同贮藏温度(室温,4 ℃,-30 ℃,-80 ℃)条件下“三华系列”(‘华硕’、‘华金’、‘华鑫’)的花粉活力。【结果】(1)3种检测方法所得“新鲜花粉”活力值各不相同,其中I_2-KI法检测花粉无活力,采用FDA荧光染色法和离体萌发法测定的花粉生活力值差异显著。‘华鑫’的新鲜花粉活力最高,‘华硕’次之,‘华金’花粉活力最低;(2)低温有利于花粉贮藏,花粉活力随贮藏时间的延长总体呈下降趋势。“三华系列”花粉品种间的适宜贮藏温度基本无显著差异,室温条件下,“三华系列”花粉活力下降速度最快,贮藏时间最短。室温下贮藏至13周后,“三华系列”花粉完全失去活力。4 ℃贮藏条件下,贮藏至第5周时,“三华系列”花粉活力40%~70%;-80 ℃适宜于花粉的长期贮藏(26周),其贮藏效果优于-30 ℃,贮藏至第26周时花粉活力在30%~40%,近似为-30 ℃贮藏条件下的2倍,花粉活力;(3)在通过对FDA荧光染色法与离体培养基法两种花粉活力检测方法进行相关性分析,发现FDA荧光染色法在4种不同贮藏温度下的测定结果与离体培养法有极显著强相关,相关系数大于0.9。【结论】I_2-KI法不适宜作为油茶花粉活力检测方法,而FDA荧光染色法可作为跟踪和测定油茶花粉活力变化的方法来表征油茶花粉潜在活力,是开展授粉工作前快速检测花粉活力的好方法;低温有利于油茶花粉的贮藏,短期内(5周)贮藏“三华系列”花粉最适宜条件是4 ℃低温贮藏,“三华系列”花粉活力在40%~70%;长期(26周)贮藏“三华系列”花粉的最适宜条件是-80 ℃低温冷冻贮藏,花粉活力在30%~40%。

语种： 中文

作者： Chen, Wangzun;He, Libing;Tian, Shiyi;Masabni, Joseph;Zhang, Riqing;...

期刊： Agronomy,2020年10(2):288- ISSN：2073-4395

通讯作者： Zou, Feng;Yuan, Deyi

作者机构： [Chen, Wangzun; He, Libing; Tian, Shiyi; Zhang, Riqing; Zou, Feng; Yuan, Deyi] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Peoples R China.;[Masabni, Joseph] Texas A&M Univ Syst, Texas A&M AgriLife Extens, 17360 Coit Rd, Dallas, TX 75252 USA.

通讯机构： [Zou, F; Yuan, DY] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Peoples R China.

关键词： chestnut burrs;cattle farming waste;waste-to-resource;composting;spicular structure rapid degradation;fluorescence parameters;plant biomass;circular economy;sustainable agriculture

摘要： In China, chestnut burrs (CB) are produced at a rate of a million tons per year as the major byproduct of chestnut orchards. It is necessary to utilize the chestnut forest green waste and convert it into a valuable seedling media for the sustainable cultivation of chestnut seedlings. In this study, we composted CB with two waste products of cattle farming, namely cow manure (CM) and bovine bone (BM). We also evaluated the potential of CB compost application in chestnut forest sustainability. Results indicated that the best combination was the addition of 15% BM and 55% CM. This combination significantly improved the composting environment by increasing pH, enhancing phosphorus concentration and mineral elements such as Ca, Na, Mg and Zn, and shortened the composting period to 38 days. This combination also resulted in the highest content of citric acid-P (109.20 times than the control treatment) and the lowest content of NH4 +–N (0.28 times than control treatment) indicating a better N and P structure of the final compost product. This combination achieved a greater degradation rate of CB cellulose (61.45%), hemicellulose (37.87%), and a more significant degradation of outer epidermis structure. When CB compost was used as a growing media, a significant decrease in photosynthesis stress of chestnut seedlings was observed, which was mainly manifested as a decrease in photochemical quenching (qP) and an increase of the maximum efficiency of PSII photochemistry under dark-adaption (Fv/Fm). Addition of 10% CB compost (in volume basis) is suggested, which resulted in the tallest chestnut seedlings (59.83 cm) with a stem diameter of 0.91 cm after six months of growth. In summary, this research provides an environmentally friendly strategy for chestnut orchard sustainability: rapid composting of CB, then immediate application as a high-quality substrate for chestnut seedlings. © 2020 by the authors.

语种： 英文

作者： Zou Feng;Zhang Xu-hui;Yuan De-yi*;Zhu Zhou-jun;Tan Lu-man;...

期刊： 光谱学与光谱分析,2019年39(1):286-291 ISSN：1000-0593

通讯作者： Yuan De-yi

作者机构： [Zou Feng; Tan Lu-man; Liu Dong-ming; Yuan De-yi; Zhang Xu-hui; Zhu Zhou-jun] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Yuan De-yi] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Hunan, Peoples R China.

关键词： 全自动间断化学分析仪;原子吸收光谱法;锥栗;花粉直感;矿质元素;主成分分析

摘要： 为探讨锥栗不同授粉组合果实中矿质元素是否也存在花粉直感效应, 应用全自动间断化学分析仪和原子吸收光谱法测定了“华栗1号”、 “华栗2号”、 “华栗3号”和“黄榛”四个锥栗主栽品种自交、 异交以及自然授粉的子代坚果中主要矿质元素的含量。 结果表明, 锥栗不同授粉组合坚果的N, P, K, Ca, Mg, Fe, Zn和Mn八种矿质元素含量差异较为显著, 其矿质元素具有明显的花粉直感效应, 尤其是铁、 锌元素受花粉直感作用的影响较大, “华栗2号”ד黄榛”授粉组合果实铁、 锌元素含量最高, 分别为162.13和41.79 μg·g-1; “黄榛”ד华栗1号”授粉组合果实中Mn元素含量最高为165.67 μg·g-1, 为锰微肥的利用提供了参考; 通过主成分分析, 评价出19个组合中在矿质元素方面表现最优的授粉组合为“华栗2号”ד黄榛”。 该研究结果可为锥栗生产上合理配置授粉树和改善果实品质提供科学的依据。 To elucidate the xenia effects of mineral elements on different pollination combinations in Castanea henryi, using the chinquapin cultivars “Huali No.1”, “Huali No.2”, “Huali No.3” and “Huangzhen” as materials, we investigated the xenia effects of mineral elements in C. henryi by auto dicsrete analyzers and atomic absorption spectrometry. Twenty combinations of self-, cross-, and natural pollination were undertaken. The results revealed that eight mineral elements of Castanea henryi seeds were significant differences. Xenia obviously has its mineral elements, especially iron and znic. The fruit of “Huali No.2”דHuangzhen” showed the highest iron and zinc content, which were 162.13 and 41.79 μg·g-1, respectively. The fruit of “Huangzhen”דHuali No.1” showed an increased manganese content of 165.67 μg·g-1, which provides a reference for the utilization of this variety as a manganese fertilizer.Through principal component analysis, the best combination was “Huali No.2”דHuangzhen” in the 19 combinations. The results can give a basis for planting design of varieties and improving fruit quality in C. henryi.

语种： 中文

作者： 李艳丽;熊欢;彭小博;邹锋;袁德义

期刊： 西南农业学报,2019年32(4):792-797 ISSN：1001-4829

作者机构： [邹锋; 袁德义; 彭小博; 李艳丽; 熊欢] 中南林业科技大学

关键词： 锥栗;嫁接苗;实生苗;叶片解剖结构;光合特性

摘要： 【目的】以2年生锥栗嫁接苗与实生苗为试材,比较分析2种苗木在叶片生长状况、形态结构及光合特性上的差异,为锥栗的良种苗木繁育提供参考.【方法】本实验主要采用Li-6400便携式光合仪测定法和石蜡切片法.【结果】①嫁接苗与实生苗的净光合速率( P_(max) ) 、光补偿点( LCP) 、光饱和点( LSP) 、表现量子效率( AQY)及叶绿素( a + b)含量( C_c )等光合指标的差异显著( P < 0.05),嫁接苗的Cc为( 4.35 ± 0.47) mg·dm~(-2) >实生苗( 2.43 ± 0.48) mg·dm~(-2),P_(max)为( 6.77 ± 0.01) μmol·m~(-2)·s~(-1) >实生苗( 5.13 ± 0.002) μmol·m~(-2)·s~(-1).②叶片的生长指标,嫁接苗的叶面积和叶干物质量较高、比叶面积较低,嫁接苗叶面积为( 44.23 ± 5.78) cm~2是实生苗( 24.55 ± 4.57 cm~2 )的1.80倍.③与实生苗相比,嫁接苗的叶片厚度、主脉厚度、表皮厚度、栅栏组织厚度、海绵组织厚度、下表皮气孔密度以及叶脉密度等结构指标均较高.其中,嫁接苗的栅栏组织厚度、主脉厚度及下表皮气孔密度分别高出实生苗35 %、38 %和50 %.【结论】锥栗苗木的光合能力与叶片结构特征关系密切,嫁接苗叶片的生长状况优于实生苗,对光的适应性和光合利用能力强于实生苗.

语种： 中文

作者： 朱周俊;袁德义;邹锋;杨华;范晓明;...

期刊： 食品科学,2019年40(2):165-170 ISSN：1002-6630

作者机构： [邹锋; 朱周俊; 肖诗鑫; 范晓明; 袁德义] 经济林培育与保护教育部重点实验室;湖北省襄阳市保康县公共检验检测中心;中南林业科技大学林学院;[杨华] 保康县公共检验检测中心;[邹昕芸; 李欣; 李果] 中南林业科技大学

关键词： 锥栗;矿质元素;因子分析;聚类分析

摘要： 为探明锥栗种仁中矿质元素含量特征规律,以30种主要锥栗农家种种仁为试材,采用自动间断分析仪与原子吸收光谱法测定N、P、K、Ca、Mg、Fe、Mn、Zn和Cu 9种矿质元素指标,运用相关性分析、因子分析和聚类分析对其矿质元素含量进行分析.结果 表明,Kolmogorov-Smirnov检验表明9种矿质元素含量数据服从正态分布,9种矿质元素平均含量顺序为N (6 873.74 mg/kg) ＞K (4 402.32 mg/kg) ＞P(1 619.16mg/kg)＞Ca (471.18mg/kg) ＞Mg (394.59mg/kg) ＞Mn (115.80mg/kg) ＞Fe (16.22mg/kg) ＞Zn (8.68 mg/kg) ＞Cu(7.59 mg/kg),大量元素中N、K、P与Ca、Mg存在显著差异(P＜0.05),微量元素中Mn与Fe、Zn、Cu差异显著(P＜0.05),变异系数范围为8.29％～54.43％;相关性分析表明各元素之间存在着复杂的关联性;因子分析结果表明,N、P、Mn、K、Cu和Fe是锥栗的特征元素,提取的6个公因子累计方差贡献率为90.572％,第1公因子方差贡献率为22.400％,主要综合了N、P和Mn 3种元素的信息,第2公因子方差贡献率为15.572％,主要综合了K和Cu 2种元素的信息,第3公因子方差贡献率为14.701％,与Fe有关,第4公因子方差贡献率为14.614％,与Mg有关,第5公因子方差贡献率为11.936％,与Ca有关,第6公因子方差贡献率为11.349％,与Zn有关,综合得分排名前5名依次为蔓榛、长芒仔、中尖嘴、材榛和小尖嘴;从元素含量角度进行聚类分析,30种锥栗农家种可分为6类.本研究结果可为进一步开展锥栗营养功能评价、锥栗育种亲本选择和锥栗食品开发等提供基本参考数据.

语种： 中文

作者： Xiong, Huan;Masabni, Joseph;Zou, Feng*;Yuan, Deyi*

期刊： International Journal of Agriculture and Biology,2019年22(3):420-426 ISSN：1560-8530

通讯作者： Zou, Feng;Yuan, Deyi

作者机构： [Xiong, Huan; Zou, Feng; Yuan, Deyi] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;[Masabni, Joseph] Texas A&M Univ Syst, Texas A&M AgriLife Extens, 1710 FM 3053 N, Overton, TX 75684 USA.

通讯机构： [Zou, F; Yuan, DY] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.

关键词： Cuttings;Fruiting body development;Fungi;Parasitic;Saprophytic

摘要： A new fungal strain was isolated from a fruiting body of Ganoderma Karst. grown in Chongyi county, Jiangxi Province, China. Morphological characterization and ITS rDNA sequence analysis suggested that the strain belongs to Ganoderma lucidum Karst., and we designated it as strain Gl8. Methods for culturing Gl8 and Castanea henryi (Skan) Rehd. Et Wils cuttings were established, and the ability of C. henryi cuttings serve as host for G. lucidum was studied in vitro. Ten days after inoculation, Gl8 grew freely on the living roots of C. henryi and the co-cultured mycelium formed fruiting body primordium, primitive stalk and primitive cap compared to free living mycelia. In inoculated cutting roots, transmission electron microscopy (TEM) showed that hyphae colonized the epidermal and cortical cells which retained their intact cell wall, cytoplasm, and organelles. There was no cell disintegration observed, and no hyphae were found in the apoplastic spaces. No mature fruiting bodies were observed in co-cultured and free-living mycelium at the end of the trial. TEM also showed that co-cultured roots had obvious cytoclasis. Our results indicated that Gl8 could inoculate living C. henryi roots and live endophytically in early stages, and C. henryi could promote Gl8 differentiation into the primordium stage of fruiting body development. © 2019 Friends Science Publishers © 2019 Friends Science Publishers

语种： 英文

作者： 王刚;袁德义;邹锋;熊欢;朱周俊;...

期刊： 植物生理与分子生物学学报,2017年53(2):264-272 ISSN：1671-3877

通讯作者： Yuan, D.-Y.

作者机构： [王刚; 袁德义; 邹锋; 熊欢; 朱周俊; 刘智强; 欧阳芬] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室, 长沙, 410004

通讯机构： Key Laboratory of Cultivation and Protection for Non-wood Forest Trees of Ministry of Education, Central South University of Forestry and Technology, Changsha, China

关键词： 锥栗;修剪;叶片质量;光合特性;产量

摘要： 为探究锥栗树体合理的修剪强度,以7年生'华栗3号'锥栗为试材,通过轻修剪(15%修剪量)、中修剪(30%修剪量)、重修剪(45%修剪量)和不修剪(对照)处理,研究了不同修剪强度对锥栗叶片质量、矿质元素含量和光合作用以及产量的影响。结果表明,不同修剪强度对锥栗不同冠层叶片质量影响不同,其中中修剪综合效果最好,在冠层上部,其单叶面积最大为72.92 cm~2,比对照增加了18.38%;在树冠中部和下部,叶片厚度均显著大于轻修剪和对照处理;中修剪的冠层下部叶片SPAD值分别较重修剪、轻修剪和对照增加4.27%、8.05%和8.29%。中修剪处理显著提高了叶片中N、P、K和Ca含量,但中修剪与重修剪Ca含量差异不显著,重修剪叶片Ca含量最高为35.56 mg·g~(-1)。中修剪的冠层上部叶片的净光合速率为12.85 μmol·m~(-2)·s~(-1)、蒸腾速率为3.75 mmol·m~(-2)·s~(-1)和气孔导度为0.18 mmol·m~(-2)·s~(-1),都是最高,较对照分别增加了20.3%、12.3%和20.0%;重修剪冠层上部胞间CO_2浓度较对照处理降低了2.32%。锥栗不同修剪强度的光合参数差异显著,中修剪锥栗树体叶片的最大光合速率、光饱和点和羧化效率显著高于其他处理,而光补偿点和暗呼吸速率显著低于其他处理。中修剪处理锥栗单株产量最高为10.25 kg,分别较重修剪、轻修剪与对照提高了9.58%、10.96%和21.61%,而重修剪处理单果重最高为7.76 g。综上,中修剪较其他处理能显著提高锥栗叶片质量与光合能力及产量,为锥栗结果期适宜的修剪强度。

语种： 中文

作者： 王刚;袁德义;邹锋;熊欢;朱周俊;...

期刊： 果树学报,2017年34(3):329-336 ISSN：1009-9980

作者机构： [王刚; 袁德义; 邹锋; 熊欢; 朱周俊; 刘智强; 欧阳芬] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室, 长沙, 410004

关键词： 锥栗;修剪;相对光照强度;产量;品质

摘要： 【目的】研究锥栗不同修剪强度的冠层内相对光照强度分布与果实产量及品质特点,探究锥栗结果期适宜的修剪强度。【方法】以7 a生'华栗3号'锥栗为试材,应用树冠分格方法,测定了锥栗不同修剪强度冠层内的相对光照强度分布、单株产量和果实品质等指标。【结果】重修剪、中修剪和轻修剪与对照相比均明显改善树冠内的光照条件,重修剪、中修剪、轻修剪冠层内相对光照强度大于30%的分别占86.11%、80.56%和75%;不同修剪强度处理中,中修剪单株产量最高,为10.25 kg,其次是轻修剪和重修剪,分别为9.29 kg和9.40 kg,而对照产量最少,为8.47 kg;重修剪在树冠中层和上层的单粒质量和淀粉含量显著高于轻修剪和对照,且含水量显著低于轻修剪和对照,中修剪和重修剪的可溶性糖和还原性糖含量显著高于轻修剪和对照。【结论】综合产量和品质两方面分析,中修剪为锥栗结果期较理想的修剪强度。

语种： 中文

作者： Feng Zou;Jinghua Duan;Huan Xiong;Deyi Yuan;Lin Zhang;...

期刊： The Open Biotechnology Journal,2017年11(1):9 -15 ISSN：1874-0707

通讯作者： Yuan, D.

作者机构： The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan 410004, China;Chinese Academy of Forestry, Beijing, 100091, China;Texas A&M AgriLife Research Center at El Paso, Texas A&M University System, 1380 A&M Circle, El Paso, TX 79927, United States

通讯机构： The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan, China

关键词： Bud differentiation;Floral organ;fruit crop;Ziziphus jujuba ‘Jinsi No.4’.

摘要： Ziziphus jujuba Mill. is one of the most important fruit crops and has been cultivated in China for more than 4000 years. Z. jujuba fruit is rich in nutritional and medicinal values. Compared to other wood fruits, Z. jujuba is unique in its flowering and fruiting characteristics. Floral buds differentiation and formation of Z. jujuba is an essential process that affects yield. Z. jujuba ‘Jinsi No.4' blooms profusely, yet its final yield is low. In this study, the floral bud differentiation and development of ‘Jinsi No.4' were examined by paraffin section. Results showed that the floral buds of ‘Jinsi No.4' differentiated in the current year and started from early April. The duration of a single flower differentiation was short, taking only 7 days for maturation of flowers buds. Floral bud differentiation of Jinsi No.4' can be divided into six stages, i.e., pre-differentiation, initial differentiation, sepal differentiation, petal differentiation, stamen differentiation, and pistil differentiation. Flower development experienced seven stages, i.e., alabastrum, alabastrum break, sepal flattening, petal flattening, stamen flattening, filament withering, and ovule swelling. Dysplasia was observed in some floral organs in Z. jujuba Jinsi No.4', suggesting that the dysplasia of floral organs may be one of the main reasons for low yields. Our findings on flower bud development in ‘Jinsi No.4' will contribute to its production and flowering management in Hunan area of southern China. © 2017 Zou et al.

语种： 英文

作者： 袁军;袁德义;谭晓风;邹锋;肖诗鑫

期刊： 中南林业科技大学学报,2017年37(5):1-6 ISSN：1673-923X

作者机构： [袁军; 袁德义; 谭晓风; 邹锋; 肖诗鑫] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室, 湖南, 长沙, 410004

关键词： 油茶;净光合速率;光合特性;日变化

摘要： 为了研究海南油茶光合效率的高低及不同无性系之间光合特性的差异,筛选高光效的海南油茶无性系,以初步筛选的5个油茶优良无性系‘海南2号’、‘海南6号’、‘海南9号’、‘海南18号’和‘海南19号’为试材,利用Li-6400xt便携式光合作用测定系统,测定了其光合作用日变化及光合-光响应曲线和光合-CO_2响应曲线,结果表明:‘海南2号’的净光合速率日变化呈单峰曲线,其它4个油茶无性系的净光合速率日变化呈双峰曲线;‘海南18号’的蒸腾速率呈单峰曲线,其它4个油茶无性系的蒸腾速率呈双峰曲线,峰值分别出现在9:00和15:00; ‘海南18号’和‘海南2号’气孔导度的日变化呈单峰曲线,‘海南9号’、‘海南19号’及‘海南6号’的日变化与P_n的日变化相一致;‘海南2号’的光饱和点和光补偿点最低,'海南19号’的光饱和点和光补偿点最高;5个油茶无性系的CO_2补偿点较高,具有C_3植物的光合特性;5个无性系P_n日变化的平均值由高到低顺序为‘海南19号,> ‘海南18号,> ‘海南9号,> ‘海南2号,〉‘海南6号,;相关分析表明,影响P_n的主要生理生态指标大小顺序依次为气孔导度 > 光合有效辐射〉蒸腾速率〉胞间CO_2浓度。

语种： 中文

作者： 王碧芳;邹锋;袁德义;袁军;肖诗鑫;...

期刊： 福建农林大学学报(自然科学版),2016年45(2):156-161 ISSN：1671-5470

作者机构： [王碧芳; 邹锋; 袁德义; 袁军; 肖诗鑫; 高超; 李泽; 彭小博] 中南林业科技大学林学院, 经济林培育与保护湖南省省部共建教育部重点实验室, 湖南, 长沙, 410004

关键词： 海南油茶;优良单株;果实经济性状;主成分分析

摘要： 对海南50个不同油茶优良单株的果实和大陆油茶‘华金’(对照)的果实经济性状指标进行测定,并采用SPSS19.0软件对其进行主成分分析.结果表明:各优株及其对照的果实经济性状的变异程度显著不同,变异系数6.73%~46.92%;用主成分抽提出5个特征值>1的主成分,累积贡献率为81.844%;二维排序图揭示了不同优良单株前3个主成分(油品质因子、果实大小因子和出籽率因子)的分布情况;50份海南油茶果实经济性状综合评价排名前10位分别为:TCPX-7、CMJL-6、QZWL-3、CMJL-4、QZCZ-3、QZCZ-4、CMZX-1、TCPX-3、QZWL-2和TCNK-1,而对照‘华金’排名21名.本研究找出了海南油茶果实经济性状评价的5个主成分,揭示了海南油茶不同优良单株的茶油品质差异.

语种： 中文

作者： Feng, Yan-zhi;Wang, Lu;Fu, Jian-min;Wuyun, Ta-na;Du, Hong-yan;...

期刊： GENES & GENOMICS,2016年38(3):275-283 ISSN：1976-9571

通讯作者： Li, Fang-dong

作者机构： [Feng, Yan-zhi; Fu, Jian-min; Wuyun, Ta-na; Wang, Lu; Du, Hong-yan; Li, Fang-dong] State Forestry Adm, Paulownia Res & Dev Ctr, Zhengzhou 450003, Henan, Peoples R China.;[Feng, Yan-zhi; Fu, Jian-min; Wuyun, Ta-na; Wang, Lu; Du, Hong-yan; Li, Fang-dong] CAF, Nontimber Forestry Res & Dev Ctr, Zhengzhou 450003, Henan, Peoples R China.;[Zou, Feng; Tan, Xiao-feng] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Li, Fang-dong] S;[Li, Fang-dong] C;State Forestry Adm, Paulownia Res & Dev Ctr, Zhengzhou 450003, Henan, Peoples R China.;CAF, Nontimber Forestry Res & Dev Ctr, Zhengzhou 450003, Henan, Peoples R China.

关键词： Eucommia ulmoides;Fatty acid biosynthesis;Quantitative real-time PCR (qPCR);RNA-Seq;Unigene

摘要： Eucommia ulmoides is a focus of considerable attention because it is used to produce gutta-percha latex and raw materials for traditional Chinese medicine. The species has also been widely planted for vegetable oil production due to the high content of alpha-linolenic acid (ALA) in the seeds. However, little was known about fatty acid biosynthesis in the seeds. Here, we present transcriptome analyses that identified genes related to the fatty acid biosynthesis pathways in the seeds collected 70 and 160 days after flowering (DAF) from two varieties: ‘Huazhong No. 6’ (ALA content of 38.7 %) and ‘Huazhong No. 10’ (ALA content of 67.6 %). De novo assembly generated 96,469 unigenes with an average length of 690 bp. Among them, 11,260 unigenes identified by functional annotation against the Kyoto Encyclopedia of Genes and Genomes database were mapped to 117 metabolic pathways. 65 unigenes involved in fatty acid biosynthesis include 5 coding for 3-oxoacyl-ACP reductase, 2 coding for β-hydroxyacyl-ACP dehydratase, 10 coding for β-ketoacyl-ACP synthase II, and 2 coding for enoyl-ACP reductase I. Expression of the nine differentially expressed unigenes coding for major enzymes in fatty acid biosynthesis was validated by real-time fluorescent quantitative PCR (qPCR) using RNA from six seed stages (70, 88, 106, 124, 142, and 160 DAF) in ‘Huazhong No. 10’. The qPCR analysis showed that expression of these 9 unigenes all changed significantly at 88 DAF. This study provides a large-scale transcriptome annotation of E. ulmoides, which is fundamental information for further characterizing gene functionals involved in the process of seed development. © 2016, The Genetics Society of Korea and Springer-Science and Media.

语种： 英文

作者： Xiong, H.;Zou, F.*;Yuan, D.;Zhang, X.;Tan, X.

期刊： New Zealand Journal of Crop and Horticultural Science,2016年44(3):192-202 ISSN：0114-0671

通讯作者： Zou, F.

作者机构： [Xiong, H.; Zou, F.; Zhang, X.; Yuan, D.; Tan, X.] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod, State Forestry Adm, Changsha, Hunan, Peoples R China.;[Xiong, H.; Zou, F.; Zhang, X.; Yuan, D.; Tan, X.] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha, Hunan, Peoples R China.;[Xiong, H.; Zou, F.; Zhang, X.; Yuan, D.; Tan, X.] Cent South Univ Forestry & Technol, Cooperat Innovat Ctr Cultivat & Utilisat Nonwood, Changsha, Hunan, Peoples R China.

通讯机构： [Zou, F.] C;Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Prod, State Forestry Adm, Changsha, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Cooperat Innovat Ctr Cultivat & Utilisat Nonwood, Changsha, Hunan, Peoples R China.

关键词： Culture medium;feijoa (Acca sellowiana cv. Unique);orthogonal design;pollen vigour;pollen tube growth

摘要： Feijoa suffers from high rates of flower and fruit abortion, and widely varying fruit characteristics, which are directly related to pollination problems. However, efficient pollination mostly depends on pollen vigour. Thus, methods to improve pollen vigour have become an important focus for both breeders and growers. An orthogonal design method (L9[3]4) was used to compare the effectiveness of key factors (e.g. concentrations of sucrose, H3BO3, CaCl2 and GA3) to determine pollen vigour, and to optimise the culture medium for in vitro pollen germination. The four factors that influenced pollen germination in order of significance are as follows: GA3 > CaCl2 > H3BO3 > sucrose. These variables influenced pollen tube growth in the following order: CaCl2 > H3BO3 > sucrose > GA3. The optimal culture medium for promoting pollen vigour was 10% sucrose, 1.95 × 10–3 mol/L H3BO3, 4 × 10–4 mol/L CaCl2 and 80 mg/L GA3. © 2016 The Royal Society of New Zealand.

语种： 英文

作者： 张旭辉;袁德义;邹锋;范晓明;唐静;...

期刊： 园艺学报,2016年43(1):61-70 ISSN：0513-353X

通讯作者： Yuan, D.-Y.

作者机构： [张旭辉; 袁德义; 邹锋; 范晓明; 唐静; 朱周俊] 中南林业科技大学, 经济林育种与栽培国家林业局重点实验室;[张旭辉; 袁德义; 邹锋; 范晓明; 唐静; 朱周俊] 经济林培育与保护教育部重点实验室, 长沙, 410004

通讯机构： [Yuan, D.-Y.] K;Key Laboratory of Non-Wood Forest Product of State Forestry Administration, China

关键词： 锥栗;授粉;坐果率;花粉直感;平均隶属度法

摘要： 为研究锥栗花粉直感效应,选用‘华栗1号'、‘华栗2号'、‘华栗3号'和‘黄榛'4个品种为试材,进行自交、异交授粉试验,以自然授粉作为对照,共20个授粉组合,分析不同授粉组合的果实成熟期、结实率及果实品质等,结果表明:锥栗在果实成熟期、坐果率、结实率、果实大小、可溶性糖、脂肪、蛋白质、直链淀粉和维生素C等方面表现明显的花粉直感效应,而在出籽率、总淀粉含量和含水量等方面无明显花粉直感效应;通过平均隶属函数法分析20个授粉组合,‘华栗2号'ב华栗3号'得分最高,‘黄榛'自交得分最低。因此,‘华栗2号'ב华栗3号'为首选授粉组合。

语种： 中文

作者： Feng Zou;Su-Juan Guo;Huan Xiong;Jing-Jing Peng;Li-Yuan Wang;...

期刊： Advance Journal of Food Science and Technology,2015年8(11):796-801 ISSN：2042-4868

通讯作者： Guo, Su-Juan

作者机构： [Xiong H.] Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China;[Wang L.-Y.; Wang J.; Peng J.-J.; Xu C.; Guo S.-J.] Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China;[Zou F.] Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China, Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China

通讯机构： [Guo, S.-J.] K;Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China

关键词： Castanea mollissima blume;Endogenous hormone;Ovary;Pollination

摘要： Chinese chestnut trees spread all over the world produce nut and chestnuts that have considerable economical value. Ovary development is an important step in nut production. Normal ovary development probably requires the coordinated action of plant hormones. Endogenous hormone status of chestnut tree, particularly IAA, IBA, GA3 and ZT, influences chestnut ovary development as well as nut yield. Changes in endogenous hormones during ovary development in chestnut cultivar 'Yanshanzaofeng' have not been thoroughly investigated. In this study, cultivar 'Yanshanzaofeng' and 'Dabanhong' were used as materials. About 50~100 pollinated female inflorescences were picked every five days (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 days, respectively) to determine the IAA, ABA, GA3 and ZT levels by HPLC. The results showed that there were differences between self-and crosspollination endogenous hormone change rules during the development of ovary. The IAA and GA3 contents in ovary of self-pollination were significantly higher than cross-pollination during pollination and fertilization period, which suggested that lower IAA and GA3 acts as a positive regulator of fertilization. In the first 40 DAP, ZT level in ovaries of self-and cross-pollination almost have the same change rule, but changed dramatically in the last 10 DAP, which suggested that ZT serves as a positive regulator for young fruit. ABA level in ovary of self-pollination was significantly higher than cross-pollination in the first 30 DAP. It seems that high ABA level was probably related with self-incompatibility in chestnut. The findings of this study could lay the scientific basis for spraying GA3, IAA and ZT during ovary development and may have the potential to improve nut yield. © Maxwell Scientific Organization, 2015.

语种： 英文

作者： Feng Zou;Su-juan Guo;Huan Xiong;Jin Wang;Xiao-na Zhang;...

期刊： Advance Journal of Food Science and Technology,2015年8(3):157-162 ISSN：2042-4868

通讯作者： Guo, Su-juan

作者机构： [Xiong H.] Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China;[Peng Y.-Q.; Zhang X.-N.; Peng J.-J.; Wang J.; Guo S.-J.] Key Laboratories for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China;[Zou F.] Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China, Key Laboratories for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China

通讯机构： [Guo, S.-J.] K;Key Laboratories for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China

关键词： Castanea mollissima blume;Nutrition;Ovary;Pollination

摘要： Chinese chestnut (Castanea mollissima Blume) has noteworthy ecological, economic and cultural importance in the Northern Hemisphere. The low yield of chestnut often affect the economic efficiency. Ovary development is an important step in nut production. Changes in nutrient contents during ovary development in chestnut cultivar 'Yanshanzaofeng' have not been thoroughly investigated. In this study, cultivar 'Yanshanzaofeng' and 'Dabanhong' were used as material. About 50~100 pollinated female inflorescences were picked every five days (5, 10, 15, 20, 25, 30, 35, 40, 45, 50 days) to determine N, P, K, fat, total soluble sugar, crude protein and starch contents. The results indicated that the contents of total soluble sugar, starch and fat increased constantly in ovaries after self-and cross-pollination, but protein, N and K contents first increased in 20 DAP (day after pollination) and after that decreased in the stage of young fruit development. The changes of P has two peak values, one was in 40 DAP and the other was in 50 DAP. P and crude protein were not significantly after pollination treatments. However, N, sugar, starch, fat and K were significantly higher in cross-pollination treatment it seems that these nutrient has a decisive role during ovary development in chestnut. The characteristics of these nutrition changes provide a basis information for spraying N, P, K etc during ovary development and may have the potential to improve nut yield. © Maxwell Scientific Organization, 2015.

语种： 英文