作者机构：
[Zhang, Donglin; Jin, Xiaoling; He, Ping; Hu, Xijun] Cent S Univ Forestry & Technol, Coll Landscape Architecture, Changsha 410004, Hunan, Peoples R China.;[Sun, Youping] Hunan Acad Forestry, Changsha 410004, Hunan, Peoples R China.;[Zhang, Donglin; Sun, Youping] Univ Maine, Dept Plant Soil & Environm Sci, Orono, ME 04469 USA.;[Jin, Xiaoling] Cent S Univ Forestry & Technol, Coll Landscape Architecture, S Shaoshan Rd 498, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Jin, Xiaoling] C;Cent S Univ Forestry & Technol, Coll Landscape Architecture, S Shaoshan Rd 498, Changsha 410004, Hunan, Peoples R China.
关键词：
6-Benzylaminopurine;callus;indole-3-butyric acid;alpha-naphthaleneacetic acid;woody plant medium
摘要：
Zelkova sinica Schneid. is a popular landscape plant in China because of its wide adaptation, strong disease resistance, large crown, and beautiful fall color. Immature embryos from Z. sinica seeds were cultured on woody plant medium (WPM) supplemented with 4.5 mu M 6-Benzylaminopurine (BA) and 5.4 mu M alpha-naphthaleneacetic acid (NAA) to induce callus, and 60% of immature embryos formed callus. The cream-white, friable, nodular callus with proembryogenic structures was then cultured on WPM containing 5.4 mu M NAA in combination with 9.0 or 11.2 mu M BA to regenerate shoots; approximately five shoots per explant were induced on 70% callus. Shoots were rooted on WPM containing 0.5 mu M indole-3-butyric acid (IBA), on which mu M 62.3% shoots developed roots with an average of 4.2 roots per shoot at 4 weeks. The regenerated plantlets were acclimatized and transplanted into the field. This protocol could be used for mass production for field plantation, genetic improvement, and germplasm exchange of Z sinica.
