The Akebia trifoliata seed oil nanoemulsion was prepared by high-pressure homogenization. The oil phase was Akebia trifo-liata seed oil, the emulsifier was Tween-80, and the co-emulsifier was propylene glycol. The emulsion size, Zeta potential, and polydis-persity index (PDI) were used as the standards to design a single-factor experiment to optimize the emulsion formulation and preparation process. It was found that the optimal preparation formula was that the mass fraction of Akebia trifoliata seed oil, Tween-80, and propylene glycol were 10%...